Isolated nucleic acid molecules encoding human transport proteins

ABSTRACT

The present invention provides amino acid sequences of peptides that are encoded by genes within the human genome, the transporter peptides of the present invention. The present invention specifically provides isolated peptide and nucleic acid molecules, methods of identifying orthologs and paralogs of the transporter peptides, and methods of identifying modulators of the transporter peptides.

RELATED APPLICATIONS

[0001] The present application claims priority to provisional application U.S. Serial No. UNKNOWN filed Feb. 26, 2000 (Atty. Docket CL001149-PROV).

FIELD OF THE INVENTION

[0002] The present invention is in the field of transporter proteins that are related to the synaptic vesicle protein subfamily, recombinant DNA molecules, and protein production. The present invention specifically provides novel peptides and proteins that effect ligand transport and nucleic acid molecules encoding such peptide and protein molecules, all of which are useful in the development of human therapeutics and diagnostic compositions and methods.

BACKGROUND OF THE INVENTION

[0003] Transporters

[0004] Transporter proteins regulate many different functions of a cell, including cell proliferation, differentiation, and signaling processes, by regulating the flow of molecules such as ions and macromolecules, into and out of cells. Transporters are found in the plasma membranes of virtually every cell in eukaryotic organisms. Transporters mediate a variety of cellular functions including regulation of membrane potentials and absorption and secretion of molecules and ion across cell membranes. When present in intracellular membranes of the Golgi apparatus and endocytic vesicles, transporters, such as chloride channels, also regulate organelle pH. For a review, see Greger, R. (1988) Annu. Rev. Physiol. 50:111-122.

[0005] Transporters are generally classified by structure and the type of mode of action. In addition, transporters are sometimes classified by the molecule type that is transported, for example, sugar transporters, chlorine channels, potassium channels, etc. There may be many classes of channels for transporting a single type of molecule (a detailed review of channel types can be found at Alexander, S. P. H. and J. A. Peters: Receptor and transporter nomenclature supplement. Trends Pharmacol. Sci., Elsevier, pp. 65-68 (1997) and http://www-biology.ucsd.edu/˜msaier/transport/titlepage2.html.

[0006] The following general classification scheme is known in the art and is followed in the present discoveries.

[0007] Channel-type transporters. Transmembrane channel proteins of this class are ubiquitously found in the membranes of all types of organisms from bacteria to higher eukaryotes. Transport systems of this type catalyze facilitated diffusion (by an energy-independent process) by passage through a transmembrane aqueous pore or channel without evidence for a carrier-mediated mechanism. These channel proteins usually consist largely of a-helical spanners, although b-strands may also be present and may even comprise the channel. However, outer membrane porin-type channel proteins are excluded from this class and are instead included in class 9.

[0008] Carrier-type transporters. Transport systems are included in this class if they utilize a carrier-mediated process to catalyze uniport (a single species is transported by facilitated diffusion), antiport (two or more species are transported in opposite directions in a tightly coupled process, not coupled to a direct form of energy other than chemiosmotic energy) and/or symport (two or more species are transported together in the same direction in a tightly coupled process, not coupled to a direct form of energy other than chemiosmotic energy).

[0009] Pyrophosphate bond hydrolysis-driven active transporters. Transport systems are included in this class if they hydrolyze pyrophosphate or the terminal pyrophosphate bond in ATP or another nucleoside triphosphate to drive the active uptake and/or extrusion of a solute or solutes. The transport protein may or may not be transiently phosphorylated, but the substrate is not phosphorylated.

[0010] PEP-dependent, phosphoryl transfer-driven group translocators. Transport systems of the bacterial phosphoenolpyruvate:sugar phosphotransferase system are included in this class. The product of the reaction, derived from extracellular sugar, is a cytoplasmic sugar-phosphate.

[0011] Decarboxylation-driven active transporters. Transport systems that drive solute (e.g., ion) uptake or extrusion by decarboxylation of a cytoplasmic substrate are included in this class.

[0012] Oxidoreduction-driven active transporters. Transport systems that drive transport of a solute (e.g., an ion) energized by the flow of electrons from a reduced substrate to an oxidized substrate are included in this class.

[0013] Light-driven active transporters. Transport systems that utilize light energy to drive transport of a solute (e.g., an ion) are included in this class.

[0014] Mechanically-driven active transporters. Transport systems are included in this class if they drive movement of a cell or organelle by allowing the flow of ions (or other solutes) through the membrane down their electrochemical gradients.

[0015] Outer-membrane porins (of b-structure). These proteins form transmembrane pores or channels that usually allow the energy independent passage of solutes across a membrane. The transmembrane portions of these proteins consist exclusively of b-strands that form a b-barrel. These porin-type proteins are found in the outer membranes of Gram-negative bacteria, mitochondria and eukaryotic plastids.

[0016] Methyltransferase-driven active transporters. A single characterized protein currently falls into this category, the Na+-transporting methyltetrahydromethanopterin:coenzyme M methyltransferase.

[0017] Non-ribosome-synthesized channel-forming peptides or peptide-like molecules. These molecules, usually chains of L- and D-amino acids as well as other small molecular building blocks such as lactate, form oligomeric transmembrane ion channels. Voltage may induce channel formation by promoting assembly of the transmembrane channel. These peptides are often made by bacteria and fungi as agents of biological warfare.

[0018] Non-Proteinaceous Transport Complexes. Ion conducting substances in biological membranes that do not consist of or are not derived from proteins or peptides fall into this category.

[0019] Functionally characterized transporters for which sequence data are lacking. Transporters of particular physiological significance will be included in this category even though a family assignment cannot be made.

[0020] Putative transporters in which no family member is an established transporter. Putative transport protein families are grouped under this number and will either be classified elsewhere when the transport function of a member becomes established, or will be eliminated from the TC classification system if the proposed transport function is disproven. These families include a member or members for which a transport function has been suggested, but evidence for such a function is not yet compelling.

[0021] Auxiliary transport proteins. Proteins that in some way facilitate transport across one or more biological membranes but do not themselves participate directly in transport are included in this class. These proteins always function in conjunction with one or more transport proteins. They may provide a function connected with energy coupling to transport, play a structural role in complex formation or serve a regulatory function.

[0022] Transporters of unknown classification. Transport protein families of unknown classification are grouped under this number and will be classified elsewhere when the transport process and energy coupling mechanism are characterized. These families include at least one member for which a transport function has been established, but either the mode of transport or the energy coupling mechanism is not known.

[0023] Ion channels

[0024] An important type of transporter is the ion channel. Ion channels regulate many different cell proliferation, differentiation, and signaling processes by regulating the flow of ions into and out of cells. Ion channels are found in the plasma membranes of virtually every cell in eukaryotic organisms. Ion channels mediate a variety of cellular functions including regulation of membrane potentials and absorption and secretion of ion across epithelial membranes. When present in intracellular membranes of the Golgi apparatus and endocytic vesicles, ion channels, such as chloride channels, also regulate organelle pH. For a review, see Greger, R. (1988) Annu. Rev. Physiol. 50:111-122.

[0025] Ion channels are generally classified by structure and the type of mode of action. For example, extracellular ligand gated channels (ELGS) are comprised of five polypeptide subunits, with each subunit having 4 membrane spanning domains, and are activated by the binding of an extracellular ligand to the channel. In addition, channels are sometimes classified by the ion type that is transported, for example, chlorine channels, potassium channels, etc. There may be many classes of channels for transporting a single type of ion (a detailed review of channel types can be found at Alexander, S. P. H. and J. A. Peters (1997). Receptor and ion channel nomenclature supplement. Trends Pharmacol. Sci., Elsevier, pp. 65-68 and http://www-biology.ucsd.edu/˜msaier/transport/toc.html.

[0026] There are many types of ion channels based on structure. For example, many ion channels fall within one of the following groups: extracellular ligand-gated channels (ELG), intracellular ligand-gated channels (ILG), inward rectifying channels (INR), intercellular (gap junction) channels, and voltage gated channels (VIC). There are additionally recognized other channel families based on ion-type transported, cellular location and drug sensitivity. Detailed information on each of these, their activity, ligand type, ion type, disease association, drugability, and other information pertinent to the present invention, is well known in the art.

[0027] Extracellular ligand-gated channels, ELGs, are generally comprised of five polypeptide subunits, Unwin, N. (1993), Cell 72: 31-41; Unwin, N. (1995), Nature 373: 37-43; Hucho, F., et al., (1996) J. Neurochem. 66: 1781-1792; Hucho, F., et al., (1996) Eur. J. Biochem. 239: 539-557; Alexander, S. P. H. and J. A. Peters (1997), Trends Pharmacol. Sci., Elsevier, pp. 4-6; 36-40; 42-44; and Xue, H. (1998) J. Mol. Evol. 47: 323-333. Each subunit has 4 membrane spanning regions: this serves as a means of identifying other members of the ELG family of proteins. ELG bind a ligand and in response modulate the flow of ions. Examples of ELG include most members of the neurotransmitter-receptor family of proteins, e.g., GABAI receptors. Other members of this family of ion channels include glycine receptors, ryandyne receptors, and ligand gated calcium channels.

[0028] The Voltage-gated Ion Channel (VIC) Superfamily

[0029] Proteins of the VIC family are ion-selective channel proteins found in a wide range of bacteria, archaea and eukaryotes Hille, B. (1992), Chapter 9: Structure of channel proteins; Chapter 20: Evolution and diversity. In: Ionic Channels of Excitable Membranes, 2nd Ed., Sinaur Assoc. Inc., Pubs., Sunderland, Mass.; Sigworth, F. J. (1993), Quart. Rev. Biophys. 27: 1-40; Salkoff, L. and T. Jegla (1995), Neuron 15: 489-492; Alexander, S. P. H. et al., (1997), Trends Pharmacol. Sci., Elsevier, pp. 76-84; Jan, L. Y. et al., (1997), Annu. Rev. Neurosci. 20: 91-123; Doyle, D. A, et al., (1998) Science 280: 69-77; Terlau, H. and W. Stühmer (1998), Naturwissenschaften 85: 437-444. They are often homo- or heterooligomeric structures with several dissimilar subunits (e.g., a1-a2-d-b Ca²⁺ channels, ab₁b₂ Na⁺ channels or (a)₄-b K⁺ channels), but the channel and the primary receptor is usually associated with the a (or a1) subunit. Functionally characterized members are specific for K⁺, Na⁺ or Ca²⁺. The K⁺ channels usually consist of homotetrameric structures with each a-subunit possessing six transmembrane spanners (TMSs). The a1 and a subunits of the Ca²⁺ and Na⁺ channels, respectively, are about four times as large and possess 4 units, each with 6 TMSs separated by a hydrophilic loop, for a total of 24 TMSs. These large channel proteins form heterotetra-unit structures equivalent to the homotetrameric structures of most K⁺ channels. All four units of the Ca²⁺ and Na⁺ channels are homologous to the single unit in the homotetrameric K⁺ channels. Ion flux via the eukaryotic channels is generally controlled by the transmembrane electrical potential (hence the designation, voltage-sensitive) although some are controlled by ligand or receptor binding.

[0030] Several putative K⁺-selective channel proteins of the VIC family have been identified in prokaryotes. The structure of one of them, the KcsA K⁺ channel of Streptomyces lividans, has been solved to 3.2 Å resolution. The protein possesses four identical subunits, each with two transmembrane helices, arranged in the shape of an inverted teepee or cone. The cone cradles the “selectivity filter” P domain in its outer end. The narrow selectivity filter is only 12 Å long, whereas the remainder of the channel is wider and lined with hydrophobic residues. A large water-filled cavity and helix dipoles stabilize K⁺ in the pore. The selectivity filter has two bound K⁺ ions about 7.5 Å apart from each other. Ion conduction is proposed to result from a balance of electrostatic attractive and repulsive forces.

[0031] In eukaryotes, each VIC family channel type has several subtypes based on pharmacological and electrophysiological data. Thus, there are five types of Ca²⁺ channels (L, N, P, Q and T). There are at least ten types of K⁺ channels, each responding in different ways to different stimuli: voltage-sensitive [Ka, Kv, Kvr, Kvs and Ksr], Ca²⁺-sensitive [BK_(Ca), IK_(Ca) and SK_(Ca)] and receptor-coupled [K_(M) and K_(ACh)]. There are at least six types of Na⁺ channels (I, II, III, μ1, H1 and PN3). Tetrameric channels from both prokaryotic and eukaryotic organisms are known in which each a-subunit possesses 2 TMSs rather than 6, and these two TMSs are homologous to TMSs 5 and 6 of the six TMS unit found in the voltage-sensitive channel proteins. KcsA of S. lividans is an example of such a 2 TMS channel protein. These channels may include the K_(Na) (Na⁺-activated) and K_(Vol) (cell volume-sensitive) K⁺ channels, as well as distantly related channels such as the Tok1 K⁺ channel of yeast, the TWIK-1 inward rectifier K⁺ channel of the mouse and the TREK-1 K⁺ channel of the mouse. Because of insufficient sequence similarity with proteins of the VIC family, inward rectifier K⁺ IRK channels (ATP-regulated; G-protein-activated) which possess a P domain and two flanking TMSs are placed in a distinct family. However, substantial sequence similarity in the P region suggests that they are homologous. The b, g and d subunits of VIC family members, when present, frequently play regulatory roles in channel activation/deactivation.

[0032] The Epithelial Na⁺ Channel (ENaC) Family

[0033] The ENaC family consists of over twenty-four sequenced proteins (Canessa, C. M., et al., (1994), Nature 367: 463-467, Le, T. and M. H. Saier, Jr. (1996), Mol. Membr. Biol. 13: 149-157; Garty, H. and L. G. Palmer (1997), Physiol. Rev. 77: 359-396; Waldmann, R., et al., (1997), Nature 386: 173-177; Darboux, I., et al., (1998), J. Biol. Chem. 273: 9424-9429; Firsov, D., et al., (1998), EMBO J. 17: 344-352; Horisberger, J. -D. (1998). Curr. Opin. Struc. Biol. 10: 443-449). All are from animals with no recognizable homologues in other eukaryotes or bacteria. The vertebrate ENaC proteins from epithelial cells cluster tightly together on the phylogenetic tree: voltage-insensitive ENaC homologues are also found in the brain. Eleven sequenced C. elegans proteins, including the degenerins, are distantly related to the vertebrate proteins as well as to each other. At least some of these proteins form part of a mechano-transducing complex for touch sensitivity. The homologous Helix aspersa (FMRF-amide)-activated Na⁺ channel is the first peptide neurotransmitter-gated ionotropic receptor to be sequenced.

[0034] Protein members of this family all exhibit the same apparent topology, each with N- and C-termini on the inside of the cell, two amphipathic transmembrane spanning segments, and a large extracellular loop. The extracellular domains contain numerous highly conserved cysteine residues. They are proposed to serve a receptor function.

[0035] Mammalian ENaC is important for the maintenance of Na⁺ balance and the regulation of blood pressure. Three homologous ENaC subunits, alpha, beta, and gamma, have been shown to assemble to form the highly Na⁺-selective channel. The stoichiometry of the three subunits is alpha₂, betal, gammal in a heterotetrameric architecture.

[0036] The Glutamate-gated Ion Channel (GIC) Family of Neurotransmitter Receptors

[0037] Members of the GIC family are heteropentameric complexes in which each of the 5 subunits is of 800-1000 amino acyl residues in length (Nakanishi, N., et al, (1990), Neuron 5: 569-581; Unwin, N. (1993), Cell 72: 31-41; Alexander, S. P. H. and J. A. Peters (1997) Trends Pharmacol. Sci., Elsevier, pp. 36-40). These subunits may span the membrane three or five times as putative a-helices with the N-termini (the glutamate-binding domains) localized extracellularly and the C-termini localized cytoplasmically. They may be distantly related to the ligand-gated ion channels, and if so, they may possess substantial b-structure in their transmembrane regions. However, homology between these two families cannot be established on the basis of sequence comparisons alone. The subunits fall into six subfamilies: a, b, g, d, e and z.

[0038] The GIC channels are divided into three types: (1) a-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA)-, (2) kainate- and (3) N-methyl-D-aspartate (NMDA)-selective glutamate receptors. Subunits of the AMPA and kainate classes exhibit 35-40% identity with each other while subunits of the NMDA receptors exhibit 22-24% identity with the former subunits. They possess large N-terminal, extracellular glutamate-binding domains that are homologous to the periplasmic glutamine and glutamate receptors of ABC-type uptake permeases of Gram-negative bacteria. All known members of the GIC family are from animals. The different channel (receptor) types exhibit distinct ion selectivities and conductance properties. The NMDA-selective large conductance channels are highly permeable to monovalent cations and Ca²⁺. The AMPA- and kainate-selective ion channels are permeable primarily to monovalent cations with only low permeability to Ca²⁺.

[0039] The Chloride Channel (ClC) Family

[0040] The ClC family is a large family consisting of dozens of sequenced proteins derived from Gram-negative and Gram-positive bacteria, cyanobacteria, archaea, yeast, plants and animals (Steinmeyer, K., et al., (1991), Nature 354: 301-304; Uchida, S., et al., (1993), J. Biol. Chem. 268: 3821-3824; Huang, M. -E., et al., (1994), J. Mol. Biol. 242: 595-598; Kawasaki, M., et al, (1994), Neuron 12: 597-604; Fisher, W. E., et al., (1995), Genomics. 29:598-606; and Foskett, J. K. (1998), Annu. Rev. Physiol. 60: 689-717). These proteins are essentially ubiquitous, although they are not encoded within genomes of Haemophilus influenzae, Mycoplasma genitalium, and Mycoplasma pneumoniae. Sequenced proteins vary in size from 395 amino acyl residues (M. jannaschii) to 988 residues (man). Several organisms contain multiple ClC family paralogues. For example, Synechocystis has two paralogues, one of 451 residues in length and the other of 899 residues. Arabidopsis thaliana has at least four sequenced paralogues, (775-792 residues), humans also have at least five paralogues (820-988 residues), and C. elegans also has at least five (810-950 residues). There are nine known members in mammals, and mutations in three of the corresponding genes cause human diseases. E. coli, Methanococcus jannaschii and Saccharomyces cerevisiae only have one ClC family member each. With the exception of the larger Synechocystis paralogue, all bacterial proteins are small (395-492 residues) while all eukaryotic proteins are larger (687-988 residues). These proteins exhibit 10-12 putative transmembrane a-helical spanners (TMSs) and appear to be present in the membrane as homodimers. While one member of the family, Torpedo ClC-O, has been reported to have two channels, one per subunit, others are believed to have just one.

[0041] All functionally characterized members of the ClC family transport chloride, some in a voltage-regulated process. These channels serve a variety of physiological functions (cell volume regulation; membrane potential stabilization; signal transduction; transepithelial transport, etc.). Different homologues in humans exhibit differing anion selectivities, i.e., ClC4 and ClC5 share a NO3⁻>Cl⁻>Br⁻>I⁻ conductance sequence, while ClC3 has an I⁻>Cl⁻ selectivity. The ClC4 and ClC5 channels and others exhibit outward rectifying currents with currents only at voltages more positive than +20 mV.

[0042] Animal Inward Rectifier K⁺ Channel (IRK-C) Family

[0043] IRK channels possess the “minimal channel-forming structure” with only a P domain, characteristic of the channel proteins of the VIC family, and two flanking transmembrane spanners (Shuck, M. E., et al., (1994), J. Biol. Chem. 269: 24261-24270; Ashen, M. D., et al., (1995), Am. J. Physiol. 268: H506-H511; Salkoff, L. and T. Jegla (1995), Neuron 15: 489-492; Aguilar-Bryan, L., et al., (1998), Physiol. Rev. 78: 227-245; Ruknudin, A., et al., (1998), J. Biol. Chem. 273: 14165-14171). They may exist in the membrane as homo- or heterooligomers. They have a greater tendency to let K⁺ flow into the cell than out. Voltage-dependence may be regulated by external K⁺, by internal Mg²⁺, by internal ATP and/or by G-proteins. The P domains of IRK channels exhibit limited sequence similarity to those of the VIC family, but this sequence similarity is insufficient to establish homology. Inward rectifiers play a role in setting cellular membrane potentials, and the closing of these channels upon depolarization permits the occurrence of long duration action potentials with a plateau phase. Inward rectifiers lack the intrinsic voltage sensing helices found in VIC family channels. In a few cases, those of Kir1.1a and Kir6.2, for example, direct interaction with a member of the ABC superfamily has been proposed to confer unique functional and regulatory properties to the heteromeric complex, including sensitivity to ATP. The SUR1 sulfonylurea receptor (spQ09428) is the ABC protein that regulates the Kir6.2 channel in response to ATP, and CFTR may regulate Kir1.1a. Mutations in SUR1 are the cause of familial persistent hyperinsulinemic hypoglycemia in infancy (PHHI), an autosomal recessive disorder characterized by unregulated insulin secretion in the pancreas.

[0044] ATP-gated Cation Channel (ACC) Family

[0045] Members of the ACC family (also called P2X receptors) respond to ATP, a functional neurotransmitter released by exocytosis from many types of neurons (North, R. A. (1996), Curr. Opin. Cell Biol. 8: 474-483; Soto, F., M. Garcia-Guzman and W. Stühmer (1997), J. Membr. Biol. 160: 91-100). They have been placed into seven groups (P2X₁-P2X₇) based on their pharmacological properties. These channels, which function at neuron-neuron and neuron-smooth muscle junctions, may play roles in the control of blood pressure and pain sensation. They may also function in lymphocyte and platelet physiology. They are found only in animals.

[0046] The proteins of the ACC family are quite similar in sequence (>35% identity), but they possess 380-1000 amino acyl residues per subunit with variability in length localized primarily to the C-terminal domains. They possess two transmembrane spanners, one about 30-50 residues from their N-termini, the other near residues 320-340. The extracellular receptor domains between these two spanners (of about 270 residues) are well conserved with numerous conserved glycyl and cysteyl residues. The hydrophilic C-termini vary in length from 25 to 240 residues. They resemble the topologically similar epithelial Na⁺ channel (ENaC) proteins in possessing (a) N- and C-termini localized intracellularly, (b) two putative transmembrane spanners, (c) a large extracellular loop domain, and (d) many conserved extracellular cysteyl residues. ACC family members are, however, not demonstrably homologous with them. ACC channels are probably hetero- or homomultimers and transport small monovalent cations (Me⁺). Some also transport Ca²⁺; a few also transport small metabolites.

[0047] The Ryanodine-Inositol 1,4,5-triphosphate Receptor Ca²⁺ Channel (RIR-CaC) Family

[0048] Ryanodine (Ry)-sensitive and inositol 1,4,5-triphosphate (IP3)-sensitive Ca² ⁺-release channels function in the release of Ca²⁺ from intracellular storage sites in animal cells and thereby regulate various Ca²⁺-dependent physiological processes (Hasan, G. et al., (1992) Development 116: 967-975; Michikawa, T., et al., (1994), J. Biol. Chem. 269: 9184-9189; Tunwell, R. E. A., (1996), Biochem. J. 318: 477-487; Lee, A. G. (1996) Biomembranes, Vol. 6, Transmembrane Receptors and Channels (A. G. Lee, ed.), JAI Press, Denver, CO., pp 291-326; Mikoshiba, K., et al., (1996) J. Biochem. Biomem. 6: 273-289). Ry receptors occur primarily in muscle cell sarcoplasmic reticular (SR) membranes, and IP3 receptors occur primarily in brain cell endoplasmic reticular (ER) membranes where they effect release of Ca²⁺ into the cytoplasm upon activation (opening) of the channel.

[0049] The Ry receptors are activated as a result of the activity of dihydropyridine-sensitive Ca²⁺ channels. The latter are members of the voltage-sensitive ion channel (VIC) family. Dihydropyridine-sensitive channels are present in the T-tubular systems of muscle tissues.

[0050] Ry receptors are homotetrameric complexes with each subunit exhibiting a molecular size of over 500,000 daltons (about 5,000 amino acyl residues). They possess C-terminal domains with six putative transmembrane a-helical spanners (TMSs). Putative pore-forming sequences occur between the fifth and sixth TMSs as suggested for members of the VIC family. The large N-terminal hydrophilic domains and the small C-terminal hydrophilic domains are localized to the cytoplasm. Low resolution 3-dimensional structural data are available. Mammals possess at least three isoforms that probably arose by gene duplication and divergence before divergence of the mammalian species. Homologues are present in humans and Caenorabditis elegans.

[0051] IP₃ receptors resemble Ry receptors in many respects. (1) They are homotetrameric complexes with each subunit exhibiting a molecular size of over 300,000 daltons (about 2,700 amino acyl residues). (2) They possess C-terminal channel domains that are homologous to those of the Ry receptors. (3) The channel domains possess six putative TMSs and a putative channel lining region between TMSs 5 and 6. (4) Both the large N-terminal domains and the smaller C-terminal tails face the cytoplasm. (5) They possess covalently linked carbohydrate on extracytoplasmic loops of the channel domains. (6) They have three currently recognized isoforms (types 1, 2, and 3) in mammals which are subject to differential regulation and have different tissue distributions.

[0052] IP₃ receptors possess three domains: N-terminal IP₃-binding domains, central coupling or regulatory domains and C-terminal channel domains. Channels are activated by IP₃ binding, and like the Ry receptors, the activities of the IP₃ receptor channels are regulated by phosphorylation of the regulatory domains, catalyzed by various protein kinases. They predominate in the endoplasmic reticular membranes of various cell types in the brain but have also been found in the plasma membranes of some nerve cells derived from a variety of tissues.

[0053] The channel domains of the Ry and IP₃ receptors comprise a coherent family that in spite of apparent structural similarities, do not show appreciable sequence similarity of the proteins of the VIC family. The Ry receptors and the IP₃ receptors cluster separately on the RIR-CaC family tree. They both have homologues in Drosophila. Based on the phylogenetic tree for the family, the family probably evolved in the following sequence: (1) A gene duplication event occurred that gave rise to Ry and IP₃ receptors in invertebrates. (2) Vertebrates evolved from invertebrates. (3) The three isoforms of each receptor arose as a result of two distinct gene duplication events. (4) These isoforms were transmitted to mammals before divergence of the mammalian species.

[0054] The Organellar Chloride Channel (0-ClC) Family

[0055] Proteins of the O-ClC family are voltage-sensitive chloride channels found in intracellular membranes but not the plasma membranes of animal cells (Landry, D, et al., (1993), J. Biol. Chem. 268: 14948-14955; Valenzuela, Set al., (1997), J. Biol. Chem. 272: 12575-12582; and Duncan, R. R., et al., (1997), J. Biol. Chem. 272: 23880-23886).

[0056] They are found in human nuclear membranes, and the bovine protein targets to the microsomes, but not the plasma membrane, when expressed in Xenopus laevis oocytes. These proteins are thought to function in the regulation of the membrane potential and in transepithelial ion absorption and secretion in the kidney. They possess two putative transmembrane a-helical spanners (TMSs) with cytoplasmic N- and C-termini and a large luminal loop that may be glycosylated. The bovine protein is 437 amino acyl residues in length and has the two putative TMSs at positions 223-239 and 367-385. The human nuclear protein is much smaller (241 residues). A C. elegans homologue is 260 residues long.

[0057] Synaptic Vesicle Proteins

[0058] The novel human protein, and encoding gene, provided by the present invention is related to the synaptic vesicle protein family of transporter proteins. The protein of the present invention shows the highest degree of similarity to rat synaptic vesicle protein 2C (SV2C), which is associated with synaptic vesicles and thought to be important for transport of neurotransmitters across membranes. The protein of the present invention is also similar to a synaptic vesicle protein known as SVOP (see Janz et al., J. Neurosci. 18 (22), 9269-9281 (1998)). SV2C is highly glycosylated, whereas SVOP is not. SVOP is expressed in brain and endocrine cells where it is localized to synaptic vesicles and microvesicles. Furthermore, SVOP is expressed in all regions of the brain, with large pyramidal neurons of the cerebral cortex being the site of highest expression. It is thought that SVOP is important for uptake of a novel component of synaptic vesicles (Janz et al., J. Neurosci. 18 (22), 9269-9281 (1998)).

[0059] Due to their importance in neural physiology, particularly in regulating transport at synaptic vesicles, novel human synaptic vesicle proteins/genes, such as provided by the present invention, are valuable as potential targets for the development of therapeutics to treat neurological diseases/disorders, as well as other diseases/disorders. Furthermore, SNPs in synaptic vesicle protein genes, such as provided by the present invention, may serve as valuable markers for the diagnosis, prognosis, prevention, and/or treatment of such diseases/disorders.

[0060] Using the information provided by the present invention, reagents such as probes/primers for detecting the SNPs or the expression of the protein/gene provided herein may be readily developed and, if desired, incorporated into kit formats such as nucleic acid arrays, primer extension reactions coupled with mass spec detection (for SNP detection), or TaqMan PCR assays (Applied Biosystems, Foster City, Calif.).

[0061] Transporter proteins, particularly members of the synaptic vesicle protein subfamily, are a major target for drug action and development. Accordingly, it is valuable to the field of pharmaceutical development to identify and characterize previously unknown transport proteins. The present invention advances the state of the art by providing previously unidentified human transport proteins.

SUMMARY OF THE INVENTION

[0062] The present invention is based in part on the identification of amino acid sequences of human transporter peptides and proteins that are related to the synaptic vesicle protein subfamily, as well as allelic variants and other mammalian orthologs thereof. These unique peptide sequences, and nucleic acid sequences that encode these peptides, can be used as models for the development of human therapeutic targets, aid in the identification of therapeutic proteins, and serve as targets for the development of human therapeutic agents that modulate transporter activity in cells and tissues that express the transporter. Experimental data as provided in FIG. 1 indicates expression in brain and muscle.

DESCRIPTION OF THE FIGURE SHEETS

[0063]FIG. 1 provides the nucleotide sequence of a cDNA molecule that encodes the transporter protein of the present invention. (SEQ ID NO:1) In addition structure and functional information is provided, such as ATG start, stop and tissue distribution, where available, that allows one to readily determine specific uses of inventions based on this molecular sequence. Experimental data as provided in FIG. 1 indicates expression in brain and muscle.

[0064]FIG. 2 provides the predicted amino acid sequence of the transporter of the present invention. (SEQ ID NO:2) In addition structure and functional information such as protein family, function, and modification sites is provided where available, allowing one to readily determine specific uses of inventions based on this molecular sequence.

[0065]FIG. 3 provides genomic sequences that span the gene encoding the transporter protein of the present invention. (SEQ ID NO:3) In addition structure and functional information, such as intron/exon structure, promoter location, etc., is provided where available, allowing one to readily determine specific uses of inventions based on this molecular sequence. As illustrated in FIG. 3, SNPs were identified at 269 different nucleotide positions.

DETAILED DESCRIPTION OF THE INVENTION

[0066] General Description

[0067] The present invention is based on the sequencing of the human genome. During the sequencing and assembly of the human genome, analysis of the sequence information revealed previously unidentified fragments of the human genome that encode peptides that share structural and/or sequence homology to protein/peptide/domains identified and characterized within the art as being a transporter protein or part of a transporter protein and are related to the synaptic vesicle protein subfamily. Utilizing these sequences, additional genomic sequences were assembled and transcript and/or cDNA sequences were isolated and characterized. Based on this analysis, the present invention provides amino acid sequences of human transporter peptides and proteins that are related to the synaptic vesicle protein subfamily, nucleic acid sequences in the form of transcript sequences, cDNA sequences and/or genomic sequences that encode these transporter peptides and proteins, nucleic acid variation (allelic information), tissue distribution of expression, and information about the closest art known protein/peptide/domain that has structural or sequence homology to the transporter of the present invention.

[0068] In addition to being previously unknown, the peptides that are provided in the present invention are selected based on their ability to be used for the development of commercially important products and services. Specifically, the present peptides are selected based on homology and/or structural relatedness to known transporter proteins of the synaptic vesicle protein subfamily and the expression pattern observed. Experimental data as provided in FIG. 1 indicates expression in brain and muscle.. The art has clearly established the commercial importance of members of this family of proteins and proteins that have expression patterns similar to that of the present gene. Some of the more specific features of the peptides of the present invention, and the uses thereof, are described herein, particularly in the Background of the Invention and in the annotation provided in the Figures, and/or are known within the art for each of the known synaptic vesicle protein family or subfamily of transporter proteins.

[0069] Specific Embodiments

[0070] Peptide Molecules

[0071] The present invention provides nucleic acid sequences that encode protein molecules that have been identified as being members of the transporter family of proteins and are related to the synaptic vesicle protein subfamily (protein sequences are provided in FIG. 2, transcript/cDNA sequences are provided in FIGS. 1 and genomic sequences are provided in FIG. 3). The peptide sequences provided in FIG. 2, as well as the obvious variants described herein, particularly allelic variants as identified herein and using the information in FIG. 3, will be referred herein as the transporter peptides of the present invention, transporter peptides, or peptides/proteins of the present invention.

[0072] The present invention provides isolated peptide and protein molecules that consist of, consist essentially of, or comprising the amino acid sequences of the transporter peptides disclosed in the FIG. 2, (encoded by the nucleic acid molecule shown in FIG. 1, transcript/cDNA or FIG. 3, genomic sequence), as well as all obvious variants of these peptides that are within the art to make and use. Some of these variants are described in detail below.

[0073] As used herein, a peptide is said to be “isolated” or “purified” when it is substantially free of cellular material or free of chemical precursors or other chemicals. The peptides of the present invention can be purified to homogeneity or other degrees of purity. The level of purification will be based on the intended use. The critical feature is that the preparation allows for the desired function of the peptide, even if in the presence of considerable amounts of other components (the features of an isolated nucleic acid molecule is discussed below).

[0074] In some uses, “substantially free of cellular material” includes preparations of the peptide having less than about 30% (by dry weight) other proteins (i.e., contaminating protein), less than about 20% other proteins, less than about 10% other proteins, or less than about 5% other proteins. When the peptide is recombinantly produced, it can also be substantially free of culture medium, i.e., culture medium represents less than about 20% of the volume of the protein preparation.

[0075] The language “substantially free of chemical precursors or other chemicals” includes preparations of the peptide in which it is separated from chemical precursors or other chemicals that are involved in its synthesis. In one embodiment, the language “substantially free of chemical precursors or other chemicals” includes preparations of the transporter peptide having less than about 30% (by dry weight) chemical precursors or other chemicals, less than about 20% chemical precursors or other chemicals, less than about 10% chemical precursors or other chemicals, or less than about 5% chemical precursors or other chemicals.

[0076] The isolated transporter peptide can be purified from cells that naturally express it, purified from cells that have been altered to express it (recombinant), or synthesized using known protein synthesis methods. Experimental data as provided in FIG. 1 indicates expression in brain and muscle. For example, a nucleic acid molecule encoding the transporter peptide is cloned into an expression vector, the expression vector introduced into a host cell and the protein expressed in the host cell. The protein can then be isolated from the cells by an appropriate purification scheme using standard protein purification techniques. Many of these techniques are described in detail below.

[0077] Accordingly, the present invention provides proteins that consist of the amino acid sequences provided in FIG. 2 (SEQ ID NO:2), for example, proteins encoded by the transcript/cDNA nucleic acid sequences shown in FIG. 1 (SEQ ID NO:1) and the genomic sequences provided in FIG. 3 (SEQ ID NO:3). The amino acid sequence of such a protein is provided in FIG. 2. A protein consists of an amino acid sequence when the amino acid sequence is the final amino acid sequence of the protein.

[0078] The present invention further provides proteins that consist essentially of the amino acid sequences provided in FIG. 2 (SEQ ID NO:2), for example, proteins encoded by the transcript/cDNA nucleic acid sequences shown in FIG. 1 (SEQ ID NO:1) and the genomic sequences provided in FIG. 3 (SEQ ID NO:3). A protein consists essentially of an amino acid sequence when such an amino acid sequence is present with only a few additional amino acid residues, for example from about 1 to about 100 or so additional residues, typically from 1 to about 20 additional residues in the final protein.

[0079] The present invention further provides proteins that comprise the amino acid sequences provided in FIG. 2 (SEQ ID NO:2), for example, proteins encoded by the transcript/cDNA nucleic acid sequences shown in FIG. 1 (SEQ ID NO:1) and the genomic sequences provided in FIG. 3 (SEQ ID NO:3). A protein comprises an amino acid sequence when the amino acid sequence is at least part of the final amino acid sequence of the protein. In such a fashion, the protein can be only the peptide or have additional amino acid molecules, such as amino acid residues (contiguous encoded sequence) that are naturally associated with it or heterologous amino acid residues/peptide sequences. Such a protein can have a few additional amino acid residues or can comprise several hundred or more additional amino acids. The preferred classes of proteins that are comprised of the transporter peptides of the present invention are the naturally occurring mature proteins. A brief description of how various types of these proteins can be made/isolated is provided below.

[0080] The transporter peptides of the present invention can be attached to heterologous sequences to form chimeric or fusion proteins. Such chimeric and fusion proteins comprise a transporter peptide operatively linked to a heterologous protein having an amino acid sequence not substantially homologous to the transporter peptide. “Operatively linked” indicates that the transporter peptide and the heterologous protein are fused in-frame. The heterologous protein can be fused to the N-terminus or C-terminus of the transporter peptide.

[0081] In some uses, the fusion protein does not affect the activity of the transporter peptide per se. For example, the fusion protein can include, but is not limited to, enzymatic fusion proteins, for example beta-galactosidase fusions, yeast two-hybrid GAL fusions, poly-His fusions, MYC-tagged, HI-tagged and Ig fusions. Such fusion proteins, particularly poly-His fusions, can facilitate the purification of recombinant transporter peptide. In certain host cells (e.g., mammalian host cells), expression and/or secretion of a protein can be increased by using a heterologous signal sequence.

[0082] A chimeric or fusion protein can be produced by standard recombinant DNA techniques. For example, DNA fragments coding for the different protein sequences are ligated together in-frame in accordance with conventional techniques. In another embodiment, the fusion gene can be synthesized by conventional techniques including automated DNA synthesizers. Alternatively, PCR amplification of gene fragments can be carried out using anchor primers which give rise to complementary overhangs between two consecutive gene fragments which can subsequently be annealed and re-amplified to generate a chimeric gene sequence (see Ausubel et al., Current Protocols in Molecular Biology, 1992). Moreover, many expression vectors are commercially available that already encode a fusion moiety (e.g., a GST protein). A transporter peptide-encoding nucleic acid can be cloned into such an expression vector such that the fusion moiety is linked in-frame to the transporter peptide.

[0083] As mentioned above, the present invention also provides and enables obvious variants of the amino acid sequence of the proteins of the present invention, such as naturally occurring mature forms of the peptide, allelic/sequence variants of the peptides, non-naturally occurring recombinantly derived variants of the peptides, and orthologs and paralogs of the peptides. Such variants can readily be generated using art-known techniques in the fields of recombinant nucleic acid technology and protein biochemistry. It is understood, however, that variants exclude any amino acid sequences disclosed prior to the invention.

[0084] Such variants can readily be identified/made using molecular techniques and the sequence information disclosed herein. Further, such variants can readily be distinguished from other peptides based on sequence and/or structural homology to the transporter peptides of the present invention. The degree of homology/identity present will be based primarily on whether the peptide is a functional variant or non-functional variant, the amount of divergence present in the paralog family and the evolutionary distance between the orthologs.

[0085] To determine the percent identity of two amino acid sequences or two nucleic acid sequences, the sequences are aligned for optimal comparison purposes (e.g., gaps can be introduced in one or both of a first and a second amino acid or nucleic acid sequence for optimal alignment and non-homologous sequences can be disregarded for comparison purposes). In a preferred embodiment, at least 30%, 40%, 50%, 60%, 70%, 80%, or 90% or more of a reference sequence is aligned for comparison purposes. The amino acid residues or nucleotides at corresponding amino acid positions or nucleotide positions are then compared. When a position in the first sequence is occupied by the same amino acid residue or nucleotide as the corresponding position in the second sequence, then the molecules are identical at that position (as used herein amino acid or nucleic acid “identity” is equivalent to amino acid or nucleic acid “homology”). The percent identity between the two sequences is a function of the number of identical positions shared by the sequences, taking into account the number of gaps, and the length of each gap, which need to be introduced for optimal alignment of the two sequences.

[0086] The comparison of sequences and determination of percent identity and similarity between two sequences can be accomplished using a mathematical algorithm. (Computational Molecular Biology, Lesk, A. M., ed., Oxford University Press, New York, 1988; Biocomputing: Informatics and Genome Projects, Smith, D. W., ed., Academic Press, New York, 1993; Computer Analysis of sequence Data, Part 1, Griffin, A. M., and Griffin, H. G., eds., Humana Press, New Jersey, 1994; Sequence Analysis in Molecular Biology, von Heinje, G., Academic Press, 1987; and Sequence Analysis Primer, Gribskov, M. and Devereux, J., eds., M Stockton Press, New York, 1991). In a preferred embodiment, the percent identity between two amino acid sequences is determined using the Needleman and Wunsch (J. Mol. Biol. (48):444-453 (1970)) algorithm which has been incorporated into the GAP program in the GCG software package (available at http://www.gcg.com), using either a Blossom 62 matrix or a PAM250 matrix, and a gap weight of 16, 14, 12, 10, 8, 6, or 4 and a length weight of 1, 2, 3, 4, 5, or 6. In yet another preferred embodiment, the percent identity between two nucleotide sequences is determined using the GAP program in the GCG software package (Devereux, J., et al., Nucleic Acids Res. 12(1):387 (1984)) (available at http://www.gcg.com), using a NWSgapdna.CMP matrix and a gap weight of 40, 50, 60, 70, or 80 and a length weight of 1, 2, 3, 4, 5, or 6. In another embodiment, the percent identity between two amino acid or nucleotide sequences is determined using the algorithm of E. Myers and W. Miller (CABIOS, 4:11-17 (1989)) which has been incorporated into the ALIGN program (version 2.0), using a PAM120 weight residue table, a gap length penalty of 12 and a gap penalty of 4.

[0087] The nucleic acid and protein sequences of the present invention can further be used as a “query sequence” to perform a search against sequence databases to, for example, identify other family members or related sequences. Such searches can be performed using the NBLAST and XBLAST programs (version 2.0) of Altschul, et al. (J. Mol. Biol. 215:403-10 (1990)). BLAST nucleotide searches can be performed with the NBLAST program, score=100, wordlength 12 to obtain nucleotide sequences homologous to the nucleic acid molecules of the invention. BLAST protein searches can be performed with the XBLAST program, score=50, wordlength=3 to obtain amino acid sequences homologous to the proteins of the invention. To obtain gapped alignments for comparison purposes, Gapped BLAST can be utilized as described in Altschul et al. (Nucleic Acids Res. 25(17):3389-3402 (1997)). When utilizing BLAST and gapped BLAST programs, the default parameters of the respective programs (e.g., XBLAST and NBLAST) can be used.

[0088] Full-length pre-processed forms, as well as mature processed forms, of proteins that comprise one of the peptides of the present invention can readily be identified as having complete sequence identity to one of the transporter peptides of the present invention as well as being encoded by the same genetic locus as the transporter peptide provided herein. The gene encoding the novel transporter protein of the present invention is located on a genome component that has been mapped to human chromosome 5 (as indicated in FIG. 3), which is supported by multiple lines of evidence, such as STS and BAC map data.

[0089] Allelic variants of a transporter peptide can readily be identified as being a human protein having a high degree (significant) of sequence homology/identity to at least a portion of the transporter peptide as well as being encoded by the same genetic locus as the transporter peptide provided herein. Genetic locus can readily be determined based on the genomic information provided in FIG. 3, such as the genomic sequence mapped to the reference human. The gene encoding the novel transporter protein of the present invention is located on a genome component that has been mapped to human chromosome 5 (as indicated in FIG. 3), which is supported by multiple lines of evidence, such as STS and BAC map data. As used herein, two proteins (or a region of the proteins) have significant homology when the amino acid sequences are typically at least about 70-80%, 80-90%, and more typically at least about 90-95% or more homologous. A significantly homologous amino acid sequence, according to the present invention, will be encoded by a nucleic acid sequence that will hybridize to a transporter peptide encoding nucleic acid molecule under stringent conditions as more fully described below.

[0090]FIG. 3 provides information on SNPs that have been found in the gene encoding the transporter protein of the present invention. SNPs were identified at 269 different nucleotide positions, including non-synonymous coding SNPs at positions 166328, 169076, 171899, and 171919. Changes in the amino acid sequence caused by these SNPs is indicated in FIG. 3 and can readily be determined using the universal genetic code and the protein sequence provided in FIG. 2 as a reference. Some of the SNPs that are located outside the ORF and in introns may affect gene transcription.

[0091] Paralogs of a transporter peptide can readily be identified as having some degree of significant sequence homology/identity to at least a portion of the transporter peptide, as being encoded by a gene from humans, and as having similar activity or function. Two proteins will typically be considered paralogs when the amino acid sequences are typically at least about 60% or greater, and more typically at least about 70% or greater homology through a given region or domain. Such paralogs will be encoded by a nucleic acid sequence that will hybridize to a transporter peptide encoding nucleic acid molecule under moderate to stringent conditions as more fully described below.

[0092] Orthologs of a transporter peptide can readily be identified as having some degree of significant sequence homology/identity to at least a portion of the transporter peptide as well as being encoded by a gene from another organism. Preferred orthologs will be isolated from mammals, preferably primates, for the development of human therapeutic targets and agents. Such orthologs will be encoded by a nucleic acid sequence that will hybridize to a transporter peptide encoding nucleic acid molecule under moderate to stringent conditions, as more fully described below, depending on the degree of relatedness of the two organisms yielding the proteins.

[0093] Non-naturally occurring variants of the transporter peptides of the present invention can readily be generated using recombinant techniques. Such variants include, but are not limited to deletions, additions and substitutions in the amino acid sequence of the transporter peptide. For example, one class of substitutions are conserved amino acid substitution. Such substitutions are those that substitute a given amino acid in a transporter peptide by another amino acid of like characteristics. Typically seen as conservative substitutions are the replacements, one for another, among the aliphatic amino acids Ala, Val, Leu, and Ile; interchange of the hydroxyl residues Ser and Thr; exchange of the acidic residues Asp and Glu; substitution between the amide residues Asn and Gln; exchange of the basic residues Lys and Arg; and replacements among the aromatic residues Phe and Tyr. Guidance concerning which amino acid changes are likely to be phenotypically silent are found in Bowie et al., Science 247:1306-1310 (1990).

[0094] Variant transporter peptides can be fully functional or can lack function in one or more activities, e.g. ability to bind ligand, ability to transport ligand, ability to mediate signaling, etc. Fully functional variants typically contain only conservative variation or variation in non-critical residues or in non-critical regions. FIG. 2 provides the result of protein analysis and can be used to identify critical domains/regions. Functional variants can also contain substitution of similar amino acids that result in no change or an insignificant change in function. Alternatively, such substitutions may positively or negatively affect function to some degree.

[0095] Non-functional variants typically contain one or more non-conservative amino acid substitutions, deletions, insertions, inversions, or truncation or a substitution, insertion, inversion, or deletion in a critical residue or critical region.

[0096] Amino acids that are essential for function can be identified by methods known in the art, such as site-directed mutagenesis or alanine-scanning mutagenesis (Cunningham et al., Science 244:1081-1085 (1989)), particularly using the results provided in FIG. 2. The latter procedure introduces single alanine mutations at every residue in the molecule. The resulting mutant molecules are then tested for biological activity such as transporter activity or in assays such as an in vitro proliferative activity. Sites that are critical for binding partner/substrate binding can also be determined by structural analysis such as crystallization, nuclear magnetic resonance or photoaffinity labeling (Smith et al, J. Mol. Biol. 224:899-904 (1992); de Vos et al. Science 255:306-312 (1992)).

[0097] The present invention further provides fragments of the transporter peptides, in addition to proteins and peptides that comprise and consist of such fragments, particularly those comprising the residues identified in FIG. 2. The fragments to which the invention pertains, however, are not to be construed as encompassing fragments that may be disclosed publicly prior to the present invention.

[0098] As used herein, a fragment comprises at least 8, 10, 12, 14, 16, or more contiguous amino acid residues from a transporter peptide. Such fragments can be chosen based on the ability to retain one or more of the biological activities of the transporter peptide or could be chosen for the ability to perform a function, e.g. bind a substrate or act as an immunogen. Particularly important fragments are biologically active fragments, peptides that are, for example, about 8 or more amino acids in length. Such fragments will typically comprise a domain or motif of the transporter peptide, e.g., active site, a transmembrane domain or a substrate-binding domain. Further, possible fragments include, but are not limited to, domain or motif containing fragments, soluble peptide fragments, and fragments containing immunogenic structures. Predicted domains and functional sites are readily identifiable by computer programs well known and readily available to those of skill in the art (e.g., PROSITE analysis). The results of one such analysis are provided in FIG. 2.

[0099] Polypeptides often contain amino acids other than the 20 amino acids commonly referred to as the 20 naturally occurring amino acids. Further, many amino acids, including the terminal amino acids, may be modified by natural processes, such as processing and other post-translational modifications, or by chemical modification techniques well known in the art. Common modifications that occur naturally in transporter peptides are described in basic texts, detailed monographs, and the research literature, and they are well known to those of skill in the art (some of these features are identified in FIG. 2).

[0100] Known modifications include, but are not limited to, acetylation, acylation, ADP-ribosylation, amidation, covalent attachment of flavin, covalent attachment of a heme moiety, covalent attachment of a nucleotide or nucleotide derivative, covalent attachment of a lipid or lipid derivative, covalent attachment of phosphotidylinositol, cross-linking, cyclization, disulfide bond formation, demethylation, formation of covalent crosslinks, formation of cystine, formation of pyroglutamate, formylation, gamma carboxylation, glycosylation, GPI anchor formation, hydroxylation, iodination, methylation, myristoylation, oxidation, proteolytic processing, phosphorylation, prenylation, racemization, selenoylation, sulfation, transfer-RNA mediated addition of amino acids to proteins such as arginylation, and ubiquitination.

[0101] Such modifications are well known to those of skill in the art and have been described in great detail in the scientific literature. Several particularly common modifications, glycosylation, lipid attachment, sulfation, gamma-carboxylation of glutamic acid residues, hydroxylation and ADP-ribosylation, for instance, are described in most basic texts, such as Proteins—Structure and Molecular Properties, 2nd Ed., T. E. Creighton, W. H. Freeman and Company, New York (1993). Many detailed reviews are available on this subject, such as by Wold, F., Posttranslational Covalent Modification of proteins, B. C. Johnson, Ed., Academic Press, New York 1-12 (1983); Seifter et al. (Meth. Enzymol. 182: 626-646 (1990)) and Rattan et al. (Ann. N.Y. Acad. Sci. 663:48-62 (1992)).

[0102] Accordingly, the transporter peptides of the present invention also encompass derivatives or analogs in which a substituted amino acid residue is not one encoded by the genetic code, in which a substituent group is included, in which the mature transporter peptide is fused with another compound, such as a compound to increase the half-life of the transporter peptide (for example, polyethylene glycol), or in which the additional amino acids are fused to the mature transporter peptide, such as a leader or secretory sequence or a sequence for purification of the mature transporter peptide or a pro-protein sequence.

[0103] Protein/Peptide Uses

[0104] The proteins of the present invention can be used in substantial and specific assays related to the functional information provided in the Figures; to raise antibodies or to elicit another immune response; as a reagent (including the labeled reagent) in assays designed to quantitatively determine levels of the protein (or its binding partner or ligand) in biological fluids; and as markers for tissues in which the corresponding protein is preferentially expressed (either constitutively or at a particular stage of tissue differentiation or development or in a disease state). Where the protein binds or potentially binds to another protein or ligand (such as, for example, in a transporter-effector protein interaction or transporter-ligand interaction), the protein can be used to identify the binding partner/ligand so as to develop a system to identify inhibitors of the binding interaction. Any or all of these uses are capable of being developed into reagent grade or kit format for commercialization as commercial products.

[0105] Methods for performing the uses listed above are well known to those skilled in the art. References disclosing such methods include “Molecular Cloning: A Laboratory Manual”, 2d ed., Cold Spring Harbor Laboratory Press, Sambrook, J., E. F. Fritsch and T. Maniatis eds., 1989, and “Methods in Enzymology: Guide to Molecular Cloning Techniques”, Academic Press, Berger, S. L. and A. R. Kimmel eds., 1987.

[0106] The potential uses of the peptides of the present invention are based primarily on the source of the protein as well as the class/action of the protein. For example, transporters isolated from humans and their human/mammalian orthologs serve as targets for identifying agents for use in mammalian therapeutic applications, e.g. a human drug, particularly in modulating a biological or pathological response in a cell or tissue that expresses the transporter. Experimental data as provided in FIG. 1 indicates that the transporter proteins of the present invention are expressed in brain and muscle, as indicated by virtual northern blot analysis. A large percentage of pharmaceutical agents are being developed that modulate the activity of transporter proteins, particularly members of the synaptic vesicle protein subfamily (see Background of the Invention). The structural and functional information provided in the Background and Figures provide specific and substantial uses for the molecules of the present invention, particularly in combination with the expression information provided in FIG. 1. Experimental data as provided in FIG. 1 indicates expression in brain and muscle. Such uses can readily be determined using the information provided herein, that known in the art and routine experimentation.

[0107] The proteins of the present invention (including variants and fragments that may have been disclosed prior to the present invention) are useful for biological assays related to transporters that are related to members of the synaptic vesicle protein subfamily. Such assays involve any of the known transporter functions or activities or properties useful for diagnosis and treatment of transporter-related conditions that are specific for the subfamily of transporters that the one of the present invention belongs to, particularly in cells and tissues that express the transporter. Experimental data as provided in FIG. 1 indicates that the transporter proteins of the present invention are expressed in brain and muscle, as indicated by virtual northern blot analysis. The proteins of the present invention are also useful in drug screening assays, in cell-based or cell-free systems ((Hodgson, Bio/technology, 1992, September 10(9);973-80). Cell-based systems can be native, i.e., cells that normally express the transporter, as a biopsy or expanded in cell culture. Experimental data as provided in FIG. 1 indicates expression in brain and muscle. In an alternate embodiment, cell-based assays involve recombinant host cells expressing the transporter protein.

[0108] The polypeptides can be used to identify compounds that modulate transporter activity of the protein in its natural state or an altered form that causes a specific disease or pathology associated with the transporter. Both the transporters of the present invention and appropriate variants and fragments can be used in high-throughput screens to assay candidate compounds for the ability to bind to the transporter. These compounds can be further screened against a functional transporter to determine the effect of the compound on the transporter activity. Further, these compounds can be tested in animal or invertebrate systems to determine activity/effectiveness. Compounds can be identified that activate (agonist) or inactivate (antagonist) the transporter to a desired degree.

[0109] Further, the proteins of the present invention can be used to screen a compound for the ability to stimulate or inhibit interaction between the transporter protein and a molecule that normally interacts with the transporter protein, e.g. a substrate or a component of the signal pathway that the transporter protein normally interacts (for example, another transporter). Such assays typically include the steps of combining the transporter protein with a candidate compound under conditions that allow the transporter protein, or fragment, to interact with the target molecule, and to detect the formation of a complex between the protein and the target or to detect the biochemical consequence of the interaction with the transporter protein and the target, such as any of the associated effects of signal transduction such as changes in membrane potential, protein phosphorylation, cAMP turnover, and adenylate cyclase activation, etc.

[0110] Candidate compounds include, for example, 1) peptides such as soluble peptides, including Ig-tailed fusion peptides and members of random peptide libraries (see, e.g., Lam et al., Nature 354:82-84 (1991); Houghten et al., Nature 354:84-86 (1991)) and combinatorial chemistry-derived molecular libraries made of D- and/or L-configuration amino acids; 2) phosphopeptides (e.g., members of random and partially degenerate, directed phosphopeptide libraries, see, e.g., Songyang et al., Cell 72:767-778 (1993)); 3) antibodies (e.g., polyclonal, monoclonal, humanized, anti-idiotypic, chimeric, and single chain antibodies as well as Fab, F(ab′)₂, Fab expression library fragments, and epitope-binding fragments of antibodies); and 4) small organic and inorganic molecules (e.g., molecules obtained from combinatorial and natural product libraries).

[0111] One candidate compound is a soluble fragment of the receptor that competes for ligand binding. Other candidate compounds include mutant transporters or appropriate fragments containing mutations that affect transporter function and thus compete for ligand. Accordingly, a fragment that competes for ligand, for example with a higher affinity, or a fragment that binds ligand but does not allow release, is encompassed by the invention.

[0112] The invention further includes other end point assays to identify compounds that modulate (stimulate or inhibit) transporter activity. The assays typically involve an assay of events in the signal transduction pathway that indicate transporter activity. Thus, the transport of a ligand, change in cell membrane potential, activation of a protein, a change in the expression of genes that are up- or down-regulated in response to the transporter protein dependent signal cascade can be assayed.

[0113] Any of the biological or biochemical functions mediated by the transporter can be used as an endpoint assay. These include all of the biochemical or biochemical/biological events described herein, in the references cited herein, incorporated by reference for these endpoint assay targets, and other functions known to those of ordinary skill in the art or that can be readily identified using the information provided in the Figures, particularly FIG. 2. Specifically, a biological function of a cell or tissues that expresses the transporter can be assayed. Experimental data as provided in FIG. 1 indicates that the transporter proteins of the present invention are expressed in brain and muscle, as indicated by virtual northern blot analysis.

[0114] Binding and/or activating compounds can also be screened by using chimeric transporter proteins in which the amino terminal extracellular domain, or parts thereof, the entire transmembrane domain or subregions, such as any of the seven transmembrane segments or any of the intracellular or extracellular loops and the carboxy terminal intracellular domain, or parts thereof, can be replaced by heterologous domains or subregions. For example, a ligand-binding region can be used that interacts with a different ligand then that which is recognized by the native transporter. Accordingly, a different set of signal transduction components is available as an end-point assay for activation. This allows for assays to be performed in other than the specific host cell from which the transporter is derived.

[0115] The proteins of the present invention are also useful in competition binding assays in methods designed to discover compounds that interact with the transporter (e.g. binding partners and/or ligands). Thus, a compound is exposed to a transporter polypeptide under conditions that allow the compound to bind or to otherwise interact with the polypeptide. Soluble transporter polypeptide is also added to the mixture. If the test compound interacts with the soluble transporter polypeptide, it decreases the amount of complex formed or activity from the transporter target. This type of assay is particularly useful in cases in which compounds are sought that interact with specific regions of the transporter. Thus, the soluble polypeptide that competes with the target transporter region is designed to contain peptide sequences corresponding to the region of interest.

[0116] To perform cell free drug screening assays, it is sometimes desirable to immobilize either the transporter protein, or fragment, or its target molecule to facilitate separation of complexes from uncomplexed forms of one or both of the proteins, as well as to accommodate automation of the assay.

[0117] Techniques for immobilizing proteins on matrices can be used in the drug screening assays. In one embodiment, a fusion protein can be provided which adds a domain that allows the protein to be bound to a matrix. For example, glutathione-S-transferase fusion proteins can be adsorbed onto glutathione sepharose beads (Sigma Chemical, St. Louis, Mo.) or glutathione derivatized microtitre plates, which are then combined with the cell lysates (e.g., ³⁵S-labeled) and the candidate compound, and the mixture incubated under conditions conducive to complex formation (e.g., at physiological conditions for salt and pH). Following incubation, the beads are washed to remove any unbound label, and the matrix immobilized and radiolabel determined directly, or in the supernatant after the complexes are dissociated. Alternatively, the complexes can be dissociated from the matrix, separated by SDS-PAGE, and the level of transporter-binding protein found in the bead fraction quantitated from the gel using standard electrophoretic techniques. For example, either the polypeptide or its target molecule can be immobilized utilizing conjugation of biotin and streptavidin using techniques well known in the art. Alternatively, antibodies reactive with the protein but which do not interfere with binding of the protein to its target molecule can be derivatized to the wells of the plate, and the protein trapped in the wells by antibody conjugation. Preparations of a transporter-binding protein and a candidate compound are incubated in the transporter protein-presenting wells and the amount of complex trapped in the well can be quantitated. Methods for detecting such complexes, in addition to those described above for the GST-immobilized complexes, include immunodetection of complexes using antibodies reactive with the transporter protein target molecule, or which are reactive with transporter protein and compete with the target molecule, as well as enzyme-linked assays which rely on detecting an enzymatic activity associated with the target molecule.

[0118] Agents that modulate one of the transporters of the present invention can be identified using one or more of the above assays, alone or in combination. It is generally preferable to use a cell-based or cell free system first and then confirm activity in an animal or other model system. Such model systems are well known in the art and can readily be employed in this context.

[0119] Modulators of transporter protein activity identified according to these drug screening assays can be used to treat a subject with a disorder mediated by the transporter pathway, by treating cells or tissues that express the transporter. Experimental data as provided in FIG. 1 indicates expression in brain and muscle. These methods of treatment include the steps of administering a modulator of transporter activity in a pharmaceutical composition to a subject in need of such treatment, the modulator being identified as described herein.

[0120] In yet another aspect of the invention, the transporter proteins can be used as “bait proteins” in a two-hybrid assay or three-hybrid assay (see, e.g., U.S. Pat. No. 5,283,317; Zervos et al. (1993) Cell 72:223-232; Madura et al. (1993) J. Biol. Chem. 268:12046-12054; Bartel et al. (1993) Biotechniques 14:920-924; Iwabuchi et al. (1993) Oncogene 8:1693-1696; and Brent WO94/10300), to identify other proteins, which bind to or interact with the transporter and are involved in transporter activity. Such transporter-binding proteins are also likely to be involved in the propagation of signals by the transporter proteins or transporter targets as, for example, downstream elements of a transporter-mediated signaling pathway. Alternatively, such transporter-binding proteins are likely to be transporter inhibitors.

[0121] The two-hybrid system is based on the modular nature of most transcription factors, which consist of separable DNA-binding and activation domains. Briefly, the assay utilizes two different DNA constructs. In one construct, the gene that codes for a transporter protein is fused to a gene encoding the DNA binding domain of a known transcription factor (e.g., GAL-4). In the other construct, a DNA sequence, from a library of DNA sequences, that encodes an unidentified protein (“prey” or “sample”) is fused to a gene that codes for the activation domain of the known transcription factor. If the “bait” and the “prey” proteins are able to interact, in vivo, forming a transporter-dependent complex, the DNA-binding and activation domains of the transcription factor are brought into close proximity. This proximity allows transcription of a reporter gene (e.g., LacZ) which is operably linked to a transcriptional regulatory site responsive to the transcription factor. Expression of the reporter gene can be detected and cell colonies containing the functional transcription factor can be isolated and used to obtain the cloned gene which encodes the protein which interacts with the transporter protein.

[0122] This invention further pertains to novel agents identified by the above-described screening assays. Accordingly, it is within the scope of this invention to further use an agent identified as described herein in an appropriate animal model. For example, an agent identified as described herein (e.g., a transporter-modulating agent, an antisense transporter nucleic acid molecule, a transporter-specific antibody, or a transporter-binding partner) can be used in an animal or other model to determine the efficacy, toxicity, or side effects of treatment with such an agent. Alternatively, an agent identified as described herein can be used in an animal or other model to determine the mechanism of action of such an agent. Furthermore, this invention pertains to uses of novel agents identified by the above-described screening assays for treatments as described herein.

[0123] The transporter proteins of the present invention are also useful to provide a target for diagnosing a disease or predisposition to disease mediated by the peptide. Accordingly, the invention provides methods for detecting the presence, or levels of, the protein (or encoding mRNA) in a cell, tissue, or organism. Experimental data as provided in FIG. 1 indicates expression in brain and muscle. The method involves contacting a biological sample with a compound capable of interacting with the transporter protein such that the interaction can be detected. Such an assay can be provided in a single detection format or a multi-detection format such as an antibody chip array.

[0124] One agent for detecting a protein in a sample is an antibody capable of selectively binding to protein. A biological sample includes tissues, cells and biological fluids isolated from a subject, as well as tissues, cells and fluids present within a subject.

[0125] The peptides of the present invention also provide targets for diagnosing active protein activity, disease, or predisposition to disease, in a patient having a variant peptide, particularly activities and conditions that are known for other members of the family of proteins to which the present one belongs. Thus, the peptide can be isolated from a biological sample and assayed for the presence of a genetic mutation that results in aberrant peptide. This includes amino acid substitution, deletion, insertion, rearrangement, (as the result of aberrant splicing events), and inappropriate post-translational modification. Analytic methods include altered electrophoretic mobility, altered tryptic peptide digest, altered transporter activity in cell-based or cell-free assay, alteration in ligand or antibody-binding pattern, altered isoelectric point, direct amino acid sequencing, and any other of the known assay techniques useful for detecting mutations in a protein. Such an assay can be provided in a single detection format or a multi-detection format such as an antibody chip array.

[0126] In vitro techniques for detection of peptide include enzyme linked immunosorbent assays (ELISAs), Western blots, immunoprecipitations and immunofluorescence using a detection reagent, such as an antibody or protein binding agent. Alternatively, the peptide can be detected in vivo in a subject by introducing into the subject a labeled anti-peptide antibody or other types of detection agent. For example, the antibody can be labeled with a radioactive marker whose presence and location in a subject can be detected by standard imaging techniques. Particularly useful are methods that detect the allelic variant of a peptide expressed in a subject and methods which detect fragments of a peptide in a sample.

[0127] The peptides are also useful in pharmacogenomic analysis. Pharmacogenomics deal with clinically significant hereditary variations in the response to drugs due to altered drug disposition and abnormal action in affected persons. See, e.g., Eichelbaum, M. (Clin. Exp. Pharmacol. Physiol. 23(10-11):983-985 (1996)), and Linder, M. W. (Clin. Chem. 43(2):254-266 (1997)). The clinical outcomes of these variations result in severe toxicity of therapeutic drugs in certain individuals or therapeutic failure of drugs in certain individuals as a result of individual variation in metabolism. Thus, the genotype of the individual can determine the way a therapeutic compound acts on the body or the way the body metabolizes the compound. Further, the activity of drug metabolizing enzymes effects both the intensity and duration of drug action. Thus, the pharmacogenomics of the individual permit the selection of effective compounds and effective dosages of such compounds for prophylactic or therapeutic treatment based on the individual's genotype. The discovery of genetic polymorphisms in some drug metabolizing enzymes has explained why some patients do not obtain the expected drug effects, show an exaggerated drug effect, or experience serious toxicity from standard drug dosages. Polymorphisms can be expressed in the phenotype of the extensive metabolizer and the phenotype of the poor metabolizer. Accordingly, genetic polymorphism may lead to allelic protein variants of the transporter protein in which one or more of the transporter functions in one population is different from those in another population. The peptides thus allow a target to ascertain a genetic predisposition that can affect treatment modality. Thus, in a ligand-based treatment, polymorphism may give rise to amino terminal extracellular domains and/or other ligand-binding binding regions that are more or less active in ligand binding, and transporter activation. Accordingly, ligand dosage would necessarily be modified to maximize the therapeutic effect within a given population containing a polymorphism. As an alternative to genotyping, specific polymorphic peptides could be identified.

[0128] The peptides are also useful for treating a disorder characterized by an absence of, inappropriate, or unwanted expression of the protein. Experimental data as provided in FIG. 1 indicates expression in brain and muscle. Accordingly, methods for treatment include the use of the transporter protein or fragments.

[0129] Antibodies

[0130] The invention also provides antibodies that selectively bind to one of the peptides of the present invention, a protein comprising such a peptide, as well as variants and fragments thereof. As used herein, an antibody selectively binds a target peptide when it binds the target peptide and does not significantly bind to unrelated proteins. An antibody is still considered to selectively bind a peptide even if it also binds to other proteins that are not substantially homologous with the target peptide so long as such proteins share homology with a fragment or domain of the peptide target of the antibody. In this case, it would be understood that antibody binding to the peptide is still selective despite some degree of cross-reactivity.

[0131] As used herein, an antibody is defined in terms consistent with that recognized within the art: they are multi-subunit proteins produced by a mammalian organism in response to an antigen challenge. The antibodies of the present invention include polyclonal antibodies and monoclonal antibodies, as well as fragments of such antibodies, including, but not limited to, Fab or F(ab′)₂, and Fv fragments.

[0132] Many methods are known for generating and/or identifying antibodies to a given target peptide. Several such methods are described by Harlow, Antibodies, Cold Spring Harbor Press, (1989).

[0133] In general, to generate antibodies, an isolated peptide is used as an immunogen and is administered to a mammalian organism, such as a rat, rabbit or mouse. The full-length protein, an antigenic peptide fragment or a fusion protein can be used. Particularly important fragments are those covering functional domains, such as the domains identified in FIG. 2, and domain of sequence homology or divergence amongst the family, such as those that can readily be identified using protein alignment methods and as presented in the Figures.

[0134] Antibodies are preferably prepared from regions or discrete fragments of the transporter proteins. Antibodies can be prepared from any region of the peptide as described herein. However, preferred regions will include those involved in function/activity and/or transporter/binding partner interaction. FIG. 2 can be used to identify particularly important regions while sequence alignment can be used to identify conserved and unique sequence fragments.

[0135] An antigenic fragment will typically comprise at least 8 contiguous amino acid residues. The antigenic peptide can comprise, however, at least 10, 12, 14, 16 or more amino acid residues. Such fragments can be selected on a physical property, such as fragments correspond to regions that are located on the surface of the protein, e.g., hydrophilic regions or can be selected based on sequence uniqueness (see FIG. 2).

[0136] Detection on an antibody of the present invention can be facilitated by coupling (i.e., physically linking) the antibody to a detectable substance. Examples of detectable substances include various enzymes, prosthetic groups, fluorescent materials, luminescent materials, bioluminescent materials, and radioactive materials. Examples of suitable enzymes include horseradish peroxidase, alkaline phosphatase, β-galactosidase, or acetylcholinesterase; examples of suitable prosthetic group complexes include streptavidin/biotin and avidin/biotin; examples of suitable fluorescent materials include umbelliferone, fluorescein, fluorescein isothiocyanate, rhodamine, dichlorotriazinylamine fluorescein, dansyl chloride or phycoerythrin; an example of a luminescent material includes luminol; examples of bioluminescent materials include luciferase, luciferin, and aequorin, and examples of suitable radioactive material include ¹²⁵I, ¹³¹I, ³⁵S or ³H.

[0137] Antibody Uses

[0138] The antibodies can be used to isolate one of the proteins of the present invention by standard techniques, such as affinity chromatography or immunoprecipitation. The antibodies can facilitate the purification of the natural protein from cells and recombinantly produced protein expressed in host cells. In addition, such antibodies are useful to detect the presence of one of the proteins of the present invention in cells or tissues to determine the pattern of expression of the protein among various tissues in an organism and over the course of normal development. Experimental data as provided in FIG. 1 indicates that the transporter proteins of the present invention are expressed in brain and muscle, as indicated by virtual northern blot analysis. Further, such antibodies can be used to detect protein in situ, in vitro, or in a cell lysate or supernatant in order to evaluate the abundance and pattern of expression. Also, such antibodies can be used to assess abnormal tissue distribution or abnormal expression during development or progression of a biological condition. Antibody detection of circulating fragments of the full length protein can be used to identify turnover.

[0139] Further, the antibodies can be used to assess expression in disease states such as in active stages of the disease or in an individual with a predisposition toward disease related to the protein's function. When a disorder is caused by an inappropriate tissue distribution, developmental expression, level of expression of the protein, or expressed/processed form, the antibody can be prepared against the normal protein. Experimental data as provided in FIG. 1 indicates expression in brain and muscle. If a disorder is characterized by a specific mutation in the protein, antibodies specific for this mutant protein can be used to assay for the presence of the specific mutant protein.

[0140] The antibodies can also be used to assess normal and aberrant subcellular localization of cells in the various tissues in an organism. Experimental data as provided in FIG. 1 indicates expression in brain and muscle. The diagnostic uses can be applied, not only in genetic testing, but also in monitoring a treatment modality. Accordingly, where treatment is ultimately aimed at correcting expression level or the presence of aberrant sequence and aberrant tissue distribution or developmental expression, antibodies directed against the protein or relevant fragments can be used to monitor therapeutic efficacy.

[0141] Additionally, antibodies are useful in pharmacogenomic analysis. Thus, antibodies prepared against polymorphic proteins can be used to identify individuals that require modified treatment modalities. The antibodies are also useful as diagnostic tools as an immunological marker for aberrant protein analyzed by electrophoretic mobility, isoelectric point, tryptic peptide digest, and other physical assays known to those in the art.

[0142] The antibodies are also useful for tissue typing. Experimental data as provided in FIG. 1 indicates expression in brain and muscle. Thus, where a specific protein has been correlated with expression in a specific tissue, antibodies that are specific for this protein can be used to identify a tissue type.

[0143] The antibodies are also useful for inhibiting protein function, for example, blocking the binding of the transporter peptide to a binding partner such as a ligand or protein binding partner. These uses can also be applied in a therapeutic context in which treatment involves inhibiting the protein's function. An antibody can be used, for example, to block binding, thus modulating (agonizing or antagonizing) the peptides activity. Antibodies can be prepared against specific fragments containing sites required for function or against intact protein that is associated with a cell or cell membrane. See FIG. 2 for structural information relating to the proteins of the present invention.

[0144] The invention also encompasses kits for using antibodies to detect the presence of a protein in a biological sample. The kit can comprise antibodies such as a labeled or labelable antibody and a compound or agent for detecting protein in a biological sample; means for determining the amount of protein in the sample; means for comparing the amount of protein in the sample with a standard; and instructions for use. Such a kit can be supplied to detect a single protein or epitope or can be configured to detect one of a multitude of epitopes, such as in an antibody detection array. Arrays are described in detail below for nucleic acid arrays and similar methods have been developed for antibody arrays.

[0145] Nucleic Acid Molecules

[0146] The present invention further provides isolated nucleic acid molecules that encode a transporter peptide or protein of the present invention (cDNA, transcript and genomic sequence). Such nucleic acid molecules will consist of, consist essentially of, or comprise a nucleotide sequence that encodes one of the transporter peptides of the present invention, an allelic variant thereof, or an ortholog or paralog thereof.

[0147] As used herein, an “isolated” nucleic acid molecule is one that is separated from other nucleic acid present in the natural source of the nucleic acid. Preferably, an “isolated” nucleic acid is free of sequences that naturally flank the nucleic acid (i.e., sequences located at the 5′ and 3′ ends of the nucleic acid) in the genomic DNA of the organism from which the nucleic acid is derived. However, there can be some flanking nucleotide sequences, for example up to about 5 KB, 4 KB, 3 KB, 2 KB, or 1 KB or less, particularly contiguous peptide encoding sequences and peptide encoding sequences within the same gene but separated by introns in the genomic sequence. The important point is that the nucleic acid is isolated from remote and unimportant flanking sequences such that it can be subjected to the specific manipulations described herein such as recombinant expression, preparation of probes and primers, and other uses specific to the nucleic acid sequences.

[0148] Moreover, an “isolated” nucleic acid molecule, such as a transcript/cDNA molecule, can be substantially free of other cellular material, or culture medium when produced by recombinant techniques, or chemical precursors or other chemicals when chemically synthesized. However, the nucleic acid molecule can be fused to other coding or regulatory sequences and still be considered isolated.

[0149] For example, recombinant DNA molecules contained in a vector are considered isolated. Further examples of isolated DNA molecules include recombinant DNA molecules maintained in heterologous host cells or purified (partially or substantially) DNA molecules in solution. Isolated RNA molecules include in vivo or in vitro RNA transcripts of the isolated DNA molecules of the present invention. Isolated nucleic acid molecules according to the present invention further include such molecules produced synthetically.

[0150] Accordingly, the present invention provides nucleic acid molecules that consist of the nucleotide sequence shown in FIG. 1 or 3 (SEQ ID NO:1, transcript sequence and SEQ ID NO:3, genomic sequence), or any nucleic acid molecule that encodes the protein provided in FIG. 2, SEQ ID NO:2. A nucleic acid molecule consists of a nucleotide sequence when the nucleotide sequence is the complete nucleotide sequence of the nucleic acid molecule.

[0151] The present invention further provides nucleic acid molecules that consist essentially of the nucleotide sequence shown in FIG. 1 or 3 (SEQ ID NO:1, transcript sequence and SEQ ID NO:3, genomic sequence), or any nucleic acid molecule that encodes the protein provided in FIG. 2, SEQ ID NO:2. A nucleic acid molecule consists essentially of a nucleotide sequence when such a nucleotide sequence is present with only a few additional nucleic acid residues in the final nucleic acid molecule.

[0152] The present invention further provides nucleic acid molecules that comprise the nucleotide sequences shown in FIG. 1 or 3 (SEQ ID NO:1, transcript sequence and SEQ ID NO:3, genomic sequence), or any nucleic acid molecule that encodes the protein provided in FIG. 2, SEQ ID NO:2. A nucleic acid molecule comprises a nucleotide sequence when the nucleotide sequence is at least part of the final nucleotide sequence of the nucleic acid molecule. In such a fashion, the nucleic acid molecule can be only the nucleotide sequence or have additional nucleic acid residues, such as nucleic acid residues that are naturally associated with it or heterologous nucleotide sequences. Such a nucleic acid molecule can have a few additional nucleotides or can comprise several hundred or more additional nucleotides. A brief description of how various types of these nucleic acid molecules can be readily made/isolated is provided below.

[0153] In FIGS. 1 and 3, both coding and non-coding sequences are provided. Because of the source of the present invention, humans genomic sequence (FIG. 3) and cDNA/transcript sequences (FIG. 1), the nucleic acid molecules in the Figures will contain genomic intronic sequences, 5′ and 3′ non-coding sequences, gene regulatory regions and non-coding intergenic sequences. In general such sequence features are either noted in FIGS. 1 and 3 or can readily be identified using computational tools known in the art. As discussed below, some of the non-coding regions, particularly gene regulatory elements such as promoters, are useful for a variety of purposes, e.g. control of heterologous gene expression, target for identifying gene activity modulating compounds, and are particularly claimed as fragments of the genomic sequence provided herein.

[0154] The isolated nucleic acid molecules can encode the mature protein plus additional amino or carboxyl-terminal amino acids, or amino acids interior to the mature peptide (when the mature form has more than one peptide chain, for instance). Such sequences may play a role in processing of a protein from precursor to a mature form, facilitate protein trafficking, prolong or shorten protein half-life or facilitate manipulation of a protein for assay or production, among other things. As generally is the case in situ, the additional amino acids may be processed away from the mature protein by cellular enzymes.

[0155] As mentioned above, the isolated nucleic acid molecules include, but are not limited to, the sequence encoding the transporter peptide alone, the sequence encoding the mature peptide and additional coding sequences, such as a leader or secretory sequence (e.g., a pre-pro or pro-protein sequence), the sequence encoding the mature peptide, with or without the additional coding sequences, plus additional non-coding sequences, for example introns and non-coding 5′ and 3′ sequences such as transcribed but non-translated sequences that play a role in transcription, mRNA processing (including splicing and polyadenylation signals), ribosome binding and stability of mRNA. In addition, the nucleic acid molecule may be fused to a marker sequence encoding, for example, a peptide that facilitates purification.

[0156] Isolated nucleic acid molecules can be in the form of RNA, such as mRNA, or in the form DNA, including cDNA and genomic DNA obtained by cloning or produced by chemical synthetic techniques or by a combination thereof. The nucleic acid, especially DNA, can be double-stranded or single-stranded. Single-stranded nucleic acid can be the coding strand (sense strand) or the non-coding strand (anti-sense strand).

[0157] The invention further provides nucleic acid molecules that encode fragments of the peptides of the present invention as well as nucleic acid molecules that encode obvious variants of the transporter proteins of the present invention that are described above. Such nucleic acid molecules may be naturally occurring, such as allelic variants (same locus), paralogs (different locus), and orthologs (different organism), or may be constructed by recombinant DNA methods or by chemical synthesis. Such non-naturally occurring variants may be made by mutagenesis techniques, including those applied to nucleic acid molecules, cells, or organisms. Accordingly, as discussed above, the variants can contain nucleotide substitutions, deletions, inversions and insertions. Variation can occur in either or both the coding and non-coding regions. The variations can produce both conservative and non-conservative amino acid substitutions.

[0158] The present invention further provides non-coding fragments of the nucleic acid molecules provided in FIGS. 1 and 3. Preferred non-coding fragments include, but are not limited to, promoter sequences, enhancer sequences, gene modulating sequences and gene termination sequences. Such fragments are useful in controlling heterologous gene expression and in developing screens to identify gene-modulating agents. A promoter can readily be identified as being 5′ to the ATG start site in the genomic sequence provided in FIG. 3.

[0159] A fragment comprises a contiguous nucleotide sequence greater than 12 or more nucleotides. Further, a fragment could at least 30, 40, 50, 100, 250 or 500 nucleotides in length. The length of the fragment will be based on its intended use. For example, the fragment can encode epitope bearing regions of the peptide, or can be useful as DNA probes and primers. Such fragments can be isolated using the known nucleotide sequence to synthesize an oligonucleotide probe. A labeled probe can then be used to screen a cDNA library, genomic DNA library, or mRNA to isolate nucleic acid corresponding to the coding region. Further, primers can be used in PCR reactions to clone specific regions of gene.

[0160] A probe/primer typically comprises substantially a purified oligonucleotide or oligonucleotide pair. The oligonucleotide typically comprises a region of nucleotide sequence that hybridizes under stringent conditions to at least about 12, 20, 25, 40, 50 or more consecutive nucleotides.

[0161] Orthologs, homologs, and allelic variants can be identified using methods well known in the art. As described in the Peptide Section, these variants comprise a nucleotide sequence encoding a peptide that is typically 60-70%, 70-80%, 80-90%, and more typically at least about 90-95% or more homologous to the nucleotide sequence shown in the Figure sheets or a fragment of this sequence. Such nucleic acid molecules can readily be identified as being able to hybridize under moderate to stringent conditions, to the nucleotide sequence shown in the Figure sheets or a fragment of the sequence. Allelic variants can readily be determined by genetic locus of the encoding gene. The gene encoding the novel transporter protein of the present invention is located on a genome component that has been mapped to human chromosome 5 (as indicated in FIG. 3), which is supported by multiple lines of evidence, such as STS and BAC map data.

[0162]FIG. 3 provides information on SNPs that have been found in the gene encoding the transporter protein of the present invention. SNPs were identified at 269 different nucleotide positions, including non-synonymous coding SNPs at positions 166328, 169076, 171899, and 171919. Changes in the amino acid sequence caused by these SNPs is indicated in FIG. 3 and can readily be determined using the universal genetic code and the protein sequence provided in FIG. 2 as a reference. Some of the SNPs that are located outside the ORF and in introns may affect gene transcription.

[0163] As used herein, the term “hybridizes under stringent conditions” is intended to describe conditions for hybridization and washing under which nucleotide sequences encoding a peptide at least 60-70% homologous to each other typically remain hybridized to each other. The conditions can be such that sequences at least about 60%, at least about 70%, or at least about 80% or more homologous to each other typically remain hybridized to each other. Such stringent conditions are known to those skilled in the art and can be found in Current Protocols in Molecular Biology, John Wiley & Sons, N.Y. (1989), 6.3.1-6.3.6. One example of stringent hybridization conditions are hybridization in 6×sodium chloride/sodium citrate (SSC) at about 45 C., followed by one or more washes in 0.2×SSC, 0.1% SDS at 50-65 C. Examples of moderate to low stringency hybridization conditions are well known in the art.

[0164] Nucleic Acid Molecule Uses

[0165] The nucleic acid molecules of the present invention are useful for probes, primers, chemical intermediates, and in biological assays. The nucleic acid molecules are useful as a hybridization probe for messenger RNA, transcript/cDNA and genomic DNA to isolate full-length cDNA and genomic clones encoding the peptide described in FIG. 2 and to isolate cDNA and genomic clones that correspond to variants (alleles, orthologs, etc.) producing the same or related peptides shown in FIG. 2. As illustrated in FIG. 3, SNPs were identified at 269 different nucleotide positions.

[0166] The probe can correspond to any sequence along the entire length of the nucleic acid molecules provided in the Figures. Accordingly, it could be derived from 5′ noncoding regions, the coding region, and 3′ noncoding regions. However, as discussed, fragments are not to be construed as encompassing fragments disclosed prior to the present invention.

[0167] The nucleic acid molecules are also useful as primers for PCR to amplify any given region of a nucleic acid molecule and are useful to synthesize antisense molecules of desired length and sequence.

[0168] The nucleic acid molecules are also useful for constructing recombinant vectors. Such vectors include expression vectors that express a portion of, or all of, the peptide sequences. Vectors also include insertion vectors, used to integrate into another nucleic acid molecule sequence, such as into the cellular genome, to alter in situ expression of a gene and/or gene product. For example, an endogenous coding sequence can be replaced via homologous recombination with all or part of the coding region containing one or more specifically introduced mutations.

[0169] The nucleic acid molecules are also useful for expressing antigenic portions of the proteins.

[0170] The nucleic acid molecules are also useful as probes for determining the chromosomal positions of the nucleic acid molecules by means of in situ hybridization methods. The gene encoding the novel transporter protein of the present invention is located on a genome component that has been mapped to human chromosome 5 (as indicated in FIG. 3), which is supported by multiple lines of evidence, such as STS and BAC map data.

[0171] The nucleic acid molecules are also useful in making vectors containing the gene regulatory regions of the nucleic acid molecules of the present invention.

[0172] The nucleic acid molecules are also useful for designing ribozymes corresponding to all, or a part, of the mRNA produced from the nucleic acid molecules described herein.

[0173] The nucleic acid molecules are also useful for making vectors that express part, or all, of the peptides.

[0174] The nucleic acid molecules are also useful for constructing host cells expressing a part, or all, of the nucleic acid molecules and peptides.

[0175] The nucleic acid molecules are also useful for constructing transgenic animals expressing all, or a part, of the nucleic acid molecules and peptides.

[0176] The nucleic acid molecules are also useful as hybridization probes for determining the presence, level, form and distribution of nucleic acid expression. Experimental data as provided in FIG. 1 indicates that the transporter proteins of the present invention are expressed in brain and muscle, as indicated by virtual northern blot analysis.

[0177] Accordingly, the probes can be used to detect the presence of, or to determine levels of, a specific nucleic acid molecule in cells, tissues, and in organisms. The nucleic acid whose level is determined can be DNA or RNA. Accordingly, probes corresponding to the peptides described herein can be used to assess expression and/or gene copy number in a given cell, tissue, or organism. These uses are relevant for diagnosis of disorders involving an increase or decrease in transporter protein expression relative to normal results.

[0178] In vitro techniques for detection of mRNA include Northern hybridizations and in situ hybridizations. In vitro techniques for detecting DNA include Southern hybridizations and in situ hybridization.

[0179] Probes can be used as a part of a diagnostic test kit for identifying cells or tissues that express a transporter protein, such as by measuring a level of a transporter-encoding nucleic acid in a sample of cells from a subject e.g., mRNA or genomic DNA, or determining if a transporter gene has been mutated. Experimental data as provided in FIG. 1 indicates that the transporter proteins of the present invention are expressed in brain and muscle, as indicated by virtual northern blot analysis.

[0180] Nucleic acid expression assays are useful for drug screening to identify compounds that modulate transporter nucleic acid expression.

[0181] The invention thus provides a method for identifying a compound that can be used to treat a disorder associated with nucleic acid expression of the transporter gene, particularly biological and pathological processes that are mediated by the transporter in cells and tissues that express it. Experimental data as provided in FIG. 1 indicates expression in brain and muscle. The method typically includes assaying the ability of the compound to modulate the expression of the transporter nucleic acid and thus identifying a compound that can be used to treat a disorder characterized by undesired transporter nucleic acid expression. The assays can be performed in cell-based and cell-free systems. Cell-based assays include cells naturally expressing the transporter nucleic acid or recombinant cells genetically engineered to express specific nucleic acid sequences.

[0182] The assay for transporter nucleic acid expression can involve direct assay of nucleic acid levels, such as mRNA levels, or on collateral compounds involved in the signal pathway. Further, the expression of genes that are up- or down-regulated in response to the transporter protein signal pathway can also be assayed. In this embodiment the regulatory regions of these genes can be operably linked to a reporter gene such as luciferase.

[0183] Thus, modulators of transporter gene expression can be identified in a method wherein a cell is contacted with a candidate compound and the expression of mRNA determined. The level of expression of transporter mRNA in the presence of the candidate compound is compared to the level of expression of transporter mRNA in the absence of the candidate compound. The candidate compound can then be identified as a modulator of nucleic acid expression based on this comparison and be used, for example to treat a disorder characterized by aberrant nucleic acid expression. When expression of mRNA is statistically significantly greater in the presence of the candidate compound than in its absence, the candidate compound is identified as a stimulator of nucleic acid expression. When nucleic acid expression is statistically significantly less in the presence of the candidate compound than in its absence, the candidate compound is identified as an inhibitor of nucleic acid expression.

[0184] The invention further provides methods of treatment, with the nucleic acid as a target, using a compound identified through drug screening as a gene modulator to modulate transporter nucleic acid expression in cells and tissues that express the transporter. Experimental data as provided in FIG. 1 indicates that the transporter proteins of the present invention are expressed in brain and muscle, as indicated by virtual northern blot analysis. Modulation includes both up-regulation (i.e. activation or agonization) or down-regulation (suppression or antagonization) or nucleic acid expression.

[0185] Alternatively, a modulator for transporter nucleic acid expression can be a small molecule or drug identified using the screening assays described herein as long as the drug or small molecule inhibits the transporter nucleic acid expression in the cells and tissues that express the protein. Experimental data as provided in FIG. 1 indicates expression in brain and muscle.

[0186] The nucleic acid molecules are also useful for monitoring the effectiveness of modulating compounds on the expression or activity of the transporter gene in clinical trials or in a treatment regimen. Thus, the gene expression pattern can serve as a barometer for the continuing effectiveness of treatment with the compound, particularly with compounds to which a patient can develop resistance. The gene expression pattern can also serve as a marker indicative of a physiological response of the affected cells to the compound. Accordingly, such monitoring would allow either increased administration of the compound or the administration of alternative compounds to which the patient has not become resistant. Similarly, if the level of nucleic acid expression falls below a desirable level, administration of the compound could be commensurately decreased.

[0187] The nucleic acid molecules are also useful in diagnostic assays for qualitative changes in transporter nucleic acid expression, and particularly in qualitative changes that lead to pathology. The nucleic acid molecules can be used to detect mutations in transporter genes and gene expression products such as mRNA. The nucleic acid molecules can be used as hybridization probes to detect naturally occurring genetic mutations in the transporter gene and thereby to determine whether a subject with the mutation is at risk for a disorder caused by the mutation. Mutations include deletion, addition, or substitution of one or more nucleotides in the gene, chromosomal rearrangement, such as inversion or transposition, modification of genomic DNA, such as aberrant methylation patterns or changes in gene copy number, such as amplification. Detection of a mutated form of the transporter gene associated with a dysfunction provides a diagnostic tool for an active disease or susceptibility to disease when the disease results from overexpression, underexpression, or altered expression of a transporter protein.

[0188] Individuals carrying mutations in the transporter gene can be detected at the nucleic acid level by a variety of techniques. FIG. 3 provides information on SNPs that have been found in the gene encoding the transporter protein of the present invention. SNPs were identified at 269 different nucleotide positions, including non-synonymous coding SNPs at positions 166328, 169076, 171899, and 171919. Changes in the amino acid sequence caused by these SNPs is indicated in FIG. 3 and can readily be determined using the universal genetic code and the protein sequence provided in FIG. 2 as a reference. Some of the SNPs that are located outside the ORF and in introns may affect gene transcription. The gene encoding the novel transporter protein of the present invention is located on a genome component that has been mapped to human chromosome 5 (as indicated in FIG. 3), which is supported by multiple lines of evidence, such as STS and BAC map data. Genomic DNA can be analyzed directly or can be amplified by using PCR prior to analysis. RNA or cDNA can be used in the same way. In some uses, detection of the mutation involves the use of a probe/primer in a polymerase chain reaction (PCR) (see, e.g. U.S. Pat. Nos. 4,683,195 and 4,683,202), such as anchor PCR or RACE PCR, or, alternatively, in a ligation chain reaction (LCR) (see, e.g., Landegran et al., Science 241:1077-1080 (1988); and Nakazawa et al., PNAS 91:360-364 (1994)), the latter of which can be particularly useful for detecting point mutations in the gene (see Abravaya et al., Nucleic Acids Res. 23:675-682 (1995)). This method can include the steps of collecting a sample of cells from a patient, isolating nucleic acid (e.g., genomic, mRNA or both) from the cells of the sample, contacting the nucleic acid sample with one or more primers which specifically hybridize to a gene under conditions such that hybridization and amplification of the gene (if present) occurs, and detecting the presence or absence of an amplification product, or detecting the size of the amplification product and comparing the length to a control sample. Deletions and insertions can be detected by a change in size of the amplified product compared to the normal genotype. Point mutations can be identified by hybridizing amplified DNA to normal RNA or antisense DNA sequences.

[0189] Alternatively, mutations in a transporter gene can be directly identified, for example, by alterations in restriction enzyme digestion patterns determined by gel electrophoresis.

[0190] Further, sequence-specific ribozymes (U.S. Pat. No. 5,498,531) can be used to score for the presence of specific mutations by development or loss of a ribozyme cleavage site. Perfectly matched sequences can be distinguished from mismatched sequences by nuclease cleavage digestion assays or by differences in melting temperature.

[0191] Sequence changes at specific locations can also be assessed by nuclease protection assays such as RNase and S1 protection or the chemical cleavage method. Furthermore, sequence differences between a mutant transporter gene and a wild-type gene can be determined by direct DNA sequencing. A variety of automated sequencing procedures can be utilized when performing the diagnostic assays (Naeve, C. W., (1995) Biotechniques 19:448), including sequencing by mass spectrometry (see, e.g., PCT International Publication No. WO 94/16101; Cohen et al., Adv. Chromatogr. 36:127-162 (1996); and Griffin et al., Appl. Biochem. Biotechnol. 38:147-159 (1993)).

[0192] Other methods for detecting mutations in the gene include methods in which protection from cleavage agents is used to detect mismatched bases in RNA/RNA or RNA/DNA duplexes (Myers et al., Science 230:1242 (1985)); Cotton et al., PNAS 85:4397 (1988); Saleeba et al., Meth. Enzymol. 217:286-295 (1992)), electrophoretic mobility of mutant and wild type nucleic acid is compared (Orita et al., PNAS 86:2766 (1989); Cotton et al., Mutat. Res. 285:125-144 (1993); and Hayashi et al., Genet. Anal. Tech. Appl. 9:73-79 (1992)), and movement of mutant or wild-type fragments in polyacrylamide gels containing a gradient of denaturant is assayed using denaturing gradient gel electrophoresis (Myers et al., Nature 313:495 (1985)). Examples of other techniques for detecting point mutations include selective oligonucleotide hybridization, selective amplification, and selective primer extension.

[0193] The nucleic acid molecules are also useful for testing an individual for a genotype that while not necessarily causing the disease, nevertheless affects the treatment modality. Thus, the nucleic acid molecules can be used to study the relationship between an individual's genotype and the individual's response to a compound used for treatment (pharmacogenomic relationship). Accordingly, the nucleic acid molecules described herein can be used to assess the mutation content of the transporter gene in an individual in order to select an appropriate compound or dosage regimen for treatment. FIG. 3 provides information on SNPs that have been found in the gene encoding the transporter protein of the present invention. SNPs were identified at 269 different nucleotide positions, including non-synonymous coding SNPs at positions 166328, 169076, 171899, and 171919. Changes in the amino acid sequence caused by these SNPs is indicated in FIG. 3 and can readily be determined using the universal genetic code and the protein sequence provided in FIG. 2 as a reference. Some of the SNPs that are located outside the ORF and in introns may affect gene transcription.

[0194] Thus nucleic acid molecules displaying genetic variations that affect treatment provide a diagnostic target that can be used to tailor treatment in an individual. Accordingly, the production of recombinant cells and animals containing these polymorphisms allow effective clinical design of treatment compounds and dosage regimens.

[0195] The nucleic acid molecules are thus useful as antisense constricts to control transporter gene expression in cells, tissues, and organisms. A DNA antisense nucleic acid molecule is designed to be complementary to a region of the gene involved in transcription, preventing transcription and hence production of transporter protein. An antisense RNA or DNA nucleic acid molecule would hybridize to the mRNA and thus block translation of mRNA into transporter protein.

[0196] Alternatively, a class of antisense molecules can be used to inactivate mRNA in order to decrease expression of transporter nucleic acid. Accordingly, these molecules can treat a disorder characterized by abnormal or undesired transporter nucleic acid expression. This technique involves cleavage by means of ribozymes containing nucleotide sequences complementary to one or more regions in the mRNA that attenuate the ability of the mRNA to be translated. Possible regions include coding regions and particularly coding regions corresponding to the catalytic and other functional activities of the transporter protein, such as ligand binding.

[0197] The nucleic acid molecules also provide vectors for gene therapy in patients containing cells that are aberrant in transporter gene expression. Thus, recombinant cells, which include the patient's cells that have been engineered ex vivo and returned to the patient, are introduced into an individual where the cells produce the desired transporter protein to treat the individual.

[0198] The invention also encompasses kits for detecting the presence of a transporter nucleic acid in a biological sample. Experimental data as provided in FIG. 1 indicates that the transporter proteins of the present invention are expressed in brain and muscle, as indicated by virtual northern blot analysis. For example, the kit can comprise reagents such as a labeled or labelable nucleic acid or agent capable of detecting transporter nucleic acid in a biological sample; means for determining the amount of transporter nucleic acid in the sample; and means for comparing the amount of transporter nucleic acid in the sample with a standard. The compound or agent can be packaged in a suitable container. The kit can further comprise instructions for using the kit to detect transporter protein mRNA or DNA.

[0199] Nucleic Acid Arrays

[0200] The present invention further provides nucleic acid detection kits, such as arrays or microarrays of nucleic acid molecules that are based on the sequence information provided in FIGS. 1 and 3 (SEQ ID NOS:1 and 3).

[0201] As used herein “Arrays” or “Microarrays” refers to an array of distinct polynucleotides or oligonucleotides synthesized on a substrate, such as paper, nylon or other type of membrane, filter, chip, glass slide, or any other suitable solid support. In one embodiment, the microarray is prepared and used according to the methods described in U.S. Pat. No. 5,837,832, Chee et al., PCT application W095/11995 (Chee et al.), Lockhart, D. J. et al. (1996; Nat. Biotech. 14: 1675-1680) and Schena, M. et al. (1996; Proc. Natl. Acad. Sci. 93: 10614-10619), all of which are incorporated herein in their entirety by reference. In other embodiments, such arrays are produced by the methods described by Brown et al., U.S. Pat. No. 5,807,522.

[0202] The microarray or detection kit is preferably composed of a large number of unique, single-stranded nucleic acid sequences, usually either synthetic antisense oligonucleotides or fragments of cDNAs, fixed to a solid support. The oligonucleotides are preferably about 6-60 nucleotides in length, more preferably 15-30 nucleotides in length, and most preferably about 20-25 nucleotides in length. For a certain type of microarray or detection kit, it may be preferable to use oligonucleotides that are only 7-20 nucleotides in length. The microarray or detection kit may contain oligonucleotides that cover the known 5′, or 3′, sequence, sequential oligonucleotides that cover the full length sequence; or unique oligonucleotides selected from particular areas along the length of the sequence. Polynucleotides used in the microarray or detection kit may be oligonucleotides that are specific to a gene or genes of interest.

[0203] In order to produce oligonucleotides to a known sequence for a microarray or detection kit, the gene(s) of interest (or an ORF identified from the contigs of the present invention) is typically examined using a computer algorithm which starts at the 5′ or at the 3′ end of the nucleotide sequence. Typical algorithms will then identify oligomers of defined length that are unique to the gene, have a GC content within a range suitable for hybridization, and lack predicted secondary structure that may interfere with hybridization. In certain situations it may be appropriate to use pairs of oligonucleotides on a microarray or detection kit. The “pairs” will be identical, except for one nucleotide that preferably is located in the center of the sequence. The second oligonucleotide in the pair (mismatched by one) serves as a control. The number of oligonucleotide pairs may range from two to one million. The oligomers are synthesized at designated areas on a substrate using a light-directed chemical process. The substrate may be paper, nylon or other type of membrane, filter, chip, glass slide or any other suitable solid support.

[0204] In another aspect, an oligonucleotide may be synthesized on the surface of the substrate by using a chemical coupling procedure and an ink jet application apparatus, as described in PCT application W095/251116 (Baldeschweiler et al.) which is incorporated herein in its entirety by reference. In another aspect, a “gridded” array analogous to a dot (or slot) blot may be used to arrange and link cDNA fragments or oligonucleotides to the surface of a substrate using a vacuum system, thermal, UV, mechanical or chemical bonding procedures. An array, such as those described above, may be produced by hand or by using available devices (slot blot or dot blot apparatus), materials (any suitable solid support), and machines (including robotic instruments), and may contain 8, 24, 96, 384, 1536, 6144 or more oligonucleotides, or any other number between two and one million which lends itself to the efficient use of commercially available instrumentation.

[0205] In order to conduct sample analysis using a microarray or detection kit, the RNA or DNA from a biological sample is made into hybridization probes. The mRNA is isolated, and cDNA is produced and used as a template to make antisense RNA (aRNA). The aRNA is amplified in the presence of fluorescent nucleotides, and labeled probes are incubated with the microarray or detection kit so that the probe sequences hybridize to complementary oligonucleotides of the microarray or detection kit. Incubation conditions are adjusted so that hybridization occurs with precise complementary matches or with various degrees of less complementarity. After removal of nonhybridized probes, a scanner is used to determine the levels and patterns of fluorescence. The scanned images are examined to determine degree of complementarity and the relative abundance of each oligonucleotide sequence on the microarray or detection kit. The biological samples may be obtained from any bodily fluids (such as blood, urine, saliva, phlegm, gastric juices, etc.), cultured cells, biopsies, or other tissue preparations. A detection system may be used to measure the absence, presence, and amount of hybridization for all of the distinct sequences simultaneously. This data may be used for large-scale correlation studies on the sequences, expression patterns, mutations, variants, or polymorphisms among samples.

[0206] Using such arrays, the present invention provides methods to identify the expression of the transporter proteins/peptides of the present invention. In detail, such methods comprise incubating a test sample with one or more nucleic acid molecules and assaying for binding of the nucleic acid molecule with components within the test sample. Such assays will typically involve arrays comprising many genes, at least one of which is a gene of the present invention and or alleles of the transporter gene of the present invention. FIG. 3 provides information on SNPs that have been found in the gene encoding the transporter protein of the present invention. SNPs were identified at 269 different nucleotide positions, including non-synonymous coding SNPs at positions 166328, 169076, 171899, and 171919. Changes in the amino acid sequence caused by these SNPs is indicated in FIG. 3 and can readily be determined using the universal genetic code and the protein sequence provided in FIG. 2 as a reference. Some of the SNPs that are located outside the ORF and in introns may affect gene transcription.

[0207] Conditions for incubating a nucleic acid molecule with a test sample vary. Incubation conditions depend on the format employed in the assay, the detection methods employed, and the type and nature of the nucleic acid molecule used in the assay. One skilled in the art will recognize that any one of the commonly available hybridization, amplification or array assay formats can readily be adapted to employ the novel fragments of the Human genome disclosed herein. Examples of such assays can be found in Chard, T, An Introduction to Radioimmunoassay and Related Techniques, Elsevier Science Publishers, Amsterdam, The Netherlands (1986); Bullock, G. R. et al., Techniques in Immunocytochemistry, Academic Press, Orlando, Fla. Vol. 1 (1982), Vol. 2 (1983), Vol. 3 (1985); Tijssen, P., Practice and Theory of Enzyme Immunoassays: Laboratory Techniques in Biochemistry and Molecular Biology, Elsevier Science Publishers, Amsterdam, The Netherlands (1985).

[0208] The test samples of the present invention include cells, protein or membrane extracts of cells. The test sample used in the above-described method will vary based on the assay format, nature of the detection method and the tissues, cells or extracts used as the sample to be assayed. Methods for preparing nucleic acid extracts or of cells are well known in the art and can be readily be adapted in order to obtain a sample that is compatible with the system utilized.

[0209] In another embodiment of the present invention, kits are provided which contain the necessary reagents to carry out the assays of the present invention.

[0210] Specifically, the invention provides a compartmentalized kit to receive, in close confinement, one or more containers which comprises: (a) a first container comprising one of the nucleic acid molecules that can bind to a fragment of the Human genome disclosed herein; and (b) one or more other containers comprising one or more of the following: wash reagents, reagents capable of detecting presence of a bound nucleic acid.

[0211] In detail, a compartmentalized kit includes any kit in which reagents are contained in separate containers. Such containers include small glass containers, plastic containers, strips of plastic, glass or paper, or arraying material such as silica. Such containers allows one to efficiently transfer reagents from one compartment to another compartment such that the samples and reagents are not cross-contaminated, and the agents or solutions of each container can be added in a quantitative fashion from one compartment to another. Such containers will include a container which will accept the test sample, a container which contains the nucleic acid probe, containers which contain wash reagents (such as phosphate buffered saline, Tris-buffers, etc.), and containers which contain the reagents used to detect the bound probe. One skilled in the art will readily recognize that the previously unidentified transporter gene of the present invention can be routinely identified using the sequence information disclosed herein can be readily incorporated into one of the established kit formats which are well known in the art, particularly expression arrays.

[0212] Vectors/host cells

[0213] The invention also provides vectors containing the nucleic acid molecules described herein. The term “vector” refers to a vehicle, preferably a nucleic acid molecule, which can transport the nucleic acid molecules. When the vector is a nucleic acid molecule, the nucleic acid molecules are covalently linked to the vector nucleic acid. With this aspect of the invention, the vector includes a plasmid, single or double stranded phage, a single or double stranded RNA or DNA viral vector, or artificial chromosome, such as a BAC, PAC, YAC, OR MAC.

[0214] A vector can be maintained in the host cell as an extrachromosomal element where it replicates and produces additional copies of the nucleic acid molecules. Alternatively, the vector may integrate into the host cell genome and produce additional copies of the nucleic acid molecules when the host cell replicates.

[0215] The invention provides vectors for the maintenance (cloning vectors) or vectors for expression (expression vectors) of the nucleic acid molecules. The vectors can function in procaryotic or eukaryotic cells or in both (shuttle vectors).

[0216] Expression vectors contain cis-acting regulatory regions that are operably linked in the vector to the nucleic acid molecules such that transcription of the nucleic acid molecules is allowed in a host cell. The nucleic acid molecules can be introduced into the host cell with a separate nucleic acid molecule capable of affecting transcription. Thus, the second nucleic acid molecule may provide a trans-acting factor interacting with the cis-regulatory control region to allow transcription of the nucleic acid molecules from the vector. Alternatively, a trans-acting factor may be supplied by the host cell. Finally, a trans-acting factor can be produced from the vector itself. It is understood, however, that in some embodiments, transcription and/or translation of the nucleic acid molecules can occur in a cell-free system.

[0217] The regulatory sequence to which the nucleic acid molecules described herein can be operably linked include promoters for directing mRNA transcription. These include, but are not limited to, the left promoter from bacteriophage λ, the lac, TRP, and TAC promoters from E. Coli, the early and late promoters from SV40, the CMV immediate early promoter, the adenovirus early and late promoters, and retrovirus long-terminal repeats.

[0218] In addition to control regions that promote transcription, expression vectors may also include regions that modulate transcription, such as repressor binding sites and enhancers. Examples include the SV40 enhancer, the cytomegalovirus immediate early enhancer, polyoma enhancer, adenovirus enhancers, and retrovirus LTR enhancers.

[0219] In addition to containing sites for transcription initiation and control, expression vectors can also contain sequences necessary for transcription termination and, in the transcribed region a ribosome binding site for translation. Other regulatory control elements for expression include initiation and termination codons as well as polyadenylation signals. The person of ordinary skill in the art would be aware of the numerous regulatory sequences that are useful in expression vectors. Such regulatory sequences are described, for example, in Sambrook et al., Molecular Cloning: A Laboratory Manual. 2nd. ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., (1989).

[0220] A variety of expression vectors can be used to express a nucleic acid molecule. Such vectors include chromosomal, episomal, and virus-derived vectors, for example vectors derived from bacterial plasmids, from bacteriophage, from yeast episomes, from yeast chromosomal elements, including yeast artificial chromosomes, from viruses such as baculoviruses, papovaviruses such as SV40, Vaccinia viruses, adenoviruses, poxviruses, pseudorabies viruses, and retroviruses. Vectors may also be derived from combinations of these sources such as those derived from plasmid and bacteriophage genetic elements, e.g. cosmids and phagemids. Appropriate cloning and expression vectors for prokaryotic and eukaryotic hosts are described in Sambrook et al., Molecular Cloning: A Laboratory Manual. 2nd. ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., (1989).

[0221] The regulatory sequence may provide constitutive expression in one or more host cells (i.e. tissue specific) or may provide for inducible expression in one or more cell types such as by temperature, nutrient additive, or exogenous factor such as a hormone or other ligand. A variety of vectors providing for constitutive and inducible expression in prokaryotic and eukaryotic hosts are well known to those of ordinary skill in the art.

[0222] The nucleic acid molecules can be inserted into the vector nucleic acid by well-known methodology. Generally, the DNA sequence that will ultimately be expressed is joined to an expression vector by cleaving the DNA sequence and the expression vector with one or more restriction enzymes and then ligating the fragments together. Procedures for restriction enzyme digestion and ligation are well known to those of ordinary skill in the art.

[0223] The vector containing the appropriate nucleic acid molecule can be introduced into an appropriate host cell for propagation or expression using well-known techniques. Bacterial cells include, but are not limited to, E. coli, Streptomyces, and Salmonella typhimurium. Eukaryotic cells include, but are not limited to, yeast, insect cells such as Drosophila, animal cells such as COS and CHO cells, and plant cells.

[0224] As described herein, it may be desirable to express the peptide as a fusion protein. Accordingly, the invention provides fusion vectors that allow for the production of the peptides. Fusion vectors can increase the expression of a recombinant protein, increase the solubility of the recombinant protein, and aid in the purification of the protein by acting for example as a ligand for affinity purification. A proteolytic cleavage site may be introduced at the junction of the fusion moiety so that the desired peptide can ultimately be separated from the fusion moiety. Proteolytic enzymes include, but are not limited to, factor Xa, thrombin, and enterotransporter. Typical fusion expression vectors include pGEX (Smith et al., Gene 67:31-40 (1988)), pMAL (New England Biolabs, Beverly, Mass.) and pRIT5 (Pharmacia, Piscataway, N.J.) which fuse glutathione S-transferase (GST), maltose E binding protein, or protein A, respectively, to the target recombinant protein. Examples of suitable inducible non-fusion E. coli expression vectors include pTrc (Amann et al., Gene 69:301-315 (1988)) and pET 11d (Studier et al., Gene Expression Technology: Methods in Enzymology 185:60-89 (1990)).

[0225] Recombinant protein expression can be maximized in host bacteria by providing a genetic background wherein the host cell has an impaired capacity to proteolytically cleave the recombinant protein. (Gottesman, S., Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, Calif. (1990)119-128). Alternatively, the sequence of the nucleic acid molecule of interest can be altered to provide preferential codon usage for a specific host cell, for example E. coli. (Wada et al., Nucleic Acids Res. 20:2111-2118 (1992)).

[0226] The nucleic acid molecules can also be expressed by expression vectors that are operative in yeast. Examples of vectors for expression in yeast e.g., S. cerevisiae include pYepSec1 (Baldari, et al., EMBO J. 6:229-234 (1987)), pMFa (Kurjan et al., Cell 30:933-943(1982)), pJRY88 (Schultz et al., Gene 54:113-123 (1987)), and pYES2 (Invitrogen Corporation, San Diego, Calif.).

[0227] The nucleic acid molecules can also be expressed in insect cells using, for example, baculovirus expression vectors. Baculovirus vectors available for expression of proteins in cultured insect cells (e.g., Sf9 cells) include the pAc series (Smith et al., Mol. Cell Biol. 3:2156-2165 (1983)) and the pVL series (Lucklow et al., Virology 170:31-39 (1989)).

[0228] In certain embodiments of the invention, the nucleic acid molecules described herein are expressed in mammalian cells using mammalian expression vectors. Examples of mammalian expression vectors include pCDM8 (Seed, B. Nature 329:840(1987)) and pMT2PC (Kaufman et al., EMBO J. 6:187-195 (1987)).

[0229] The expression vectors listed herein are provided by way of example only of the well-known vectors available to those of ordinary skill in the art that would be useful to express the nucleic acid molecules. The person of ordinary skill in the art would be aware of other vectors suitable for maintenance propagation or expression of the nucleic acid molecules described herein. These are found for example in Sambrook, J., Fritsh, E. F., and Maniatis, T. Molecular Cloning: A Laboratory Manual. 2nd, ed., Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1989.

[0230] The invention also encompasses vectors in which the nucleic acid sequences described herein are cloned into the vector in reverse orientation, but operably linked to a regulatory sequence that permits transcription of antisense RNA. Thus, an antisense transcript can be produced to all, or to a portion, of the nucleic acid molecule sequences described herein, including both coding and non-coding regions. Expression of this antisense RNA is subject to each of the parameters described above in relation to expression of the sense RNA (regulatory sequences, constitutive or inducible expression, tissue-specific expression).

[0231] The invention also relates to recombinant host cells containing the vectors described herein. Host cells therefore include prokaryotic cells, lower eukaryotic cells such as yeast, other eukaryotic cells such as insect cells, and higher eukaryotic cells such as mammalian cells.

[0232] The recombinant host cells are prepared by introducing the vector constructs described herein into the cells by techniques readily available to the person of ordinary skill in the art. These include, but are not limited to, calcium phosphate transfection, DEAE-dextran-mediated transfection, cationic lipid-mediated transfection, electroporation, transduction, infection, lipofection, and other techniques such as those found in Sambrook, et al. (Molecular Cloning: A Laboratory Manual. 2nd, ed., Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1989).

[0233] Host cells can contain more than one vector. Thus, different nucleotide sequences can be introduced on different vectors of the same cell. Similarly, the nucleic acid molecules can be introduced either alone or with other nucleic acid molecules that are not related to the nucleic acid molecules such as those providing trans-acting factors for expression vectors. When more than one vector is introduced into a cell, the vectors can be introduced independently, co-introduced or joined to the nucleic acid molecule vector.

[0234] In the case of bacteriophage and viral vectors, these can be introduced into cells as packaged or encapsulated virus by standard procedures for infection and transduction. Viral vectors can be replication-competent or replication-defective. In the case in which viral replication is defective, replication will occur in host cells providing functions that complement the defects.

[0235] Vectors generally include selectable markers that enable the selection of the subpopulation of cells that contain the recombinant vector constructs. The marker can be contained in the same vector that contains the nucleic acid molecules described herein or may be on a separate vector. Markers include tetracycline or ampicillin-resistance genes for prokaryotic host cells and dihydrofolate reductase or neomycin resistance for eukaryotic host cells. However, any marker that provides selection for a phenotypic trait will be effective.

[0236] While the mature proteins can be produced in bacteria, yeast, mammalian cells, and other cells under the control of the appropriate regulatory sequences, cell-free transcription and translation systems can also be used to produce these proteins using RNA derived from the DNA constructs described herein.

[0237] Where secretion of the peptide is desired, which is difficult to achieve with multi-transmembrane domain containing proteins such as transporters, appropriate secretion signals are incorporated into the vector. The signal sequence can be endogenous to the peptides or heterologous to these peptides.

[0238] Where the peptide is not secreted into the medium, which is typically the case with transporters, the protein can be isolated from the host cell by standard disruption procedures, including freeze thaw, sonication, mechanical disruption, use of lysing agents and the like. The peptide can then be recovered and purified by well-known purification methods including ammonium sulfate precipitation, acid extraction, anion or cationic exchange chromatography, phosphocellulose chromatography, hydrophobic-interaction chromatography, affinity chromatography, hydroxylapatite chromatography, lectin chromatography, or high performance liquid chromatography.

[0239] It is also understood that depending upon the host cell in recombinant production of the peptides described herein, the peptides can have various glycosylation patterns, depending upon the cell, or maybe non-glycosylated as when produced in bacteria. In addition, the peptides may include an initial modified methionine in some cases as a result of a host-mediated process.

[0240] Uses of vectors and host cells

[0241] The recombinant host cells expressing the peptides described herein have a variety of uses. First, the cells are useful for producing a transporter protein or peptide that can be further purified to produce desired amounts of transporter protein or fragments. Thus, host cells containing expression vectors are useful for peptide production.

[0242] Host cells are also useful for conducting cell-based assays involving the transporter protein or transporter protein fragments, such as those described above as well as other formats known in the art. Thus, a recombinant host cell expressing a native transporter protein is useful for assaying compounds that stimulate or inhibit transporter protein function.

[0243] Host cells are also useful for identifying transporter protein mutants in which these functions are affected. If the mutants naturally occur and give rise to a pathology, host cells containing the mutations are useful to assay compounds that have a desired effect on the mutant transporter protein (for example, stimulating or inhibiting function) which may not be indicated by their effect on the native transporter protein.

[0244] Genetically engineered host cells can be further used to produce non-human transgenic animals. A transgenic animal is preferably a mammal, for example a rodent, such as a rat or mouse, in which one or more of the cells of the animal include a transgene. A transgene is exogenous DNA that is integrated into the genome of a cell from which a transgenic animal develops and which remains in the genome of the mature animal in one or more cell types or tissues of the transgenic animal. These animals are useful for studying the function of a transporter protein and identifying and evaluating modulators of transporter protein activity. Other examples of transgenic animals include non-human primates, sheep, dogs, cows, goats, chickens, and amphibians.

[0245] A transgenic animal can be produced by introducing nucleic acid into the male pronuclei of a fertilized oocyte, e.g., by microinjection, retroviral infection, and allowing the oocyte to develop in a pseudopregnant female foster animal. Any of the transporter protein nucleotide sequences can be introduced as a transgene into the genome of a non-human animal, such as a mouse.

[0246] Any of the regulatory or other sequences useful in expression vectors can form part of the transgenic sequence. This includes intronic sequences and polyadenylation signals, if not already included. A tissue-specific regulatory sequence(s) can be operably linked to the transgene to direct expression of the transporter protein to particular cells.

[0247] Methods for generating transgenic animals via embryo manipulation and microinjection, particularly animals such as mice, have become conventional in the art and are described, for example, in U.S. Pat. Nos. 4,736,866 and 4,870,009, both by Leder et al., U.S. Pat. No. 4,873,191 by Wagner et al. and in Hogan, B., Manipulating the Mouse Embryo, (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1986). Similar methods are used for production of other transgenic animals. A transgenic founder animal can be identified based upon the presence of the transgene in its genome and/or expression of transgenic mRNA in tissues or cells of the animals. A transgenic founder animal can then be used to breed additional animals carrying the transgene. Moreover, transgenic animals carrying a transgene can further be bred to other transgenic animals carrying other transgenes. A transgenic animal also includes animals in which the entire animal or tissues in the animal have been produced using the homologously recombinant host cells described herein.

[0248] In another embodiment, transgenic non-human animals can be produced which contain selected systems that allow for regulated expression of the transgene. One example of such a system is the cre/loxP recombinase system of bacteriophage P1. For a description of the cre/loxP recombinase system, see, e.g., Lakso et al. PNAS 89:6232-6236 (1992). Another example of a recombinase system is the FLP recombinase system of S. cerevisiae (O'Gorman et al. Science 251:1351-1355 (1991). If a cre/loxP recombinase system is used to regulate expression of the transgene, animals containing transgenes encoding both the Cre recombinase and a selected protein is required. Such animals can be provided through the construction of “double” transgenic animals, e.g., by mating two transgenic animals, one containing a transgene encoding a selected protein and the other containing a transgene encoding a recombinase.

[0249] Clones of the non-human transgenic animals described herein can also be produced according to the methods described in Wilmut, I. et al. Nature 385:810-813 (1997) and PCT International Publication Nos. WO 97/07668 and WO 97/07669. In brief, a cell, e.g., a somatic cell, from the transgenic animal can be isolated and induced to exit the growth cycle and enter G₀ phase. The quiescent cell can then be fused, e.g., through the use of electrical pulses, to an enucleated oocyte from an animal of the same species from which the quiescent cell is isolated. The reconstructed oocyte is then cultured such that it develops to morula or blastocyst and then transferred to pseudopregnant female foster animal. The offspring born of this female foster animal will be a clone of the animal from which the cell, e.g., the somatic cell, is isolated.

[0250] Transgenic animals containing recombinant cells that express the peptides described herein are useful to conduct the assays described herein in an in vivo context. Accordingly, the various physiological factors that are present in vivo and that could effect ligand binding, transporter protein activation, and signal transduction, may not be evident from in vitro cell-free or cell-based assays. Accordingly, it is useful to provide non-human transgenic animals to assay in vivo transporter protein function, including ligand interaction, the effect of specific mutant transporter proteins on transporter protein function and ligand interaction, and the effect of chimeric transporter proteins. It is also possible to assess the effect of null mutations, that is mutations that substantially or completely eliminate one or more transporter protein functions.

[0251] All publications and patents mentioned in the above specification are herein incorporated by reference. Various modifications and variations of the described method and system of the invention will be apparent to those skilled in the art without departing from the scope and spirit of the invention. Although the invention has been described in connection with specific preferred embodiments, it should be understood that the invention as claimed should not be unduly limited to such specific embodiments. Indeed, various modifications of the above-described modes for carrying out the invention which are obvious to those skilled in the field of molecular biology or related fields are intended to be within the scope of the following claims.

1 4 1 2210 DNA Human 1 agcggccgcg aacgcgccct ttatatggaa gactcttaca aggataggac ttcactgatg 60 aagggtgcca aggacattgc cagagaggtg aagaaacaaa cagtaaagaa ggtgaatcaa 120 gctgtggacc gagcccagga tgaatacacc cagaggtcct acagtcggtt ccaagatgaa 180 gaagatgatg atgactacta cccggctgga gaaacctata atggtgaggc caacgatgac 240 gaaggctcaa gtgaagccac tgaggggcat gatgaagatg atgagatcta tgagggggag 300 tatcagggca tccccagtat gaaccaagcg aaggacagca tcgtgtcagt ggggcagccc 360 aagggcgatg agtacaagga ccggcgggag ctggaatcag aaaggagagc tgacgaggaa 420 gagttagccc agcagtatga gctgataatc caagaatgcg gtcatggtcg ttttcagtgg 480 gcccctttct tcgtcctggg catggctctt atggcagacg gtgtagaggt gtttgtcgtt 540 ggcttcgtgt tacccagtgc tgagacagac ctctgcatcc caaattcagg atctggatgg 600 ctaggcagca tagtgtacct cgggatgatg gtgggggcgt tcttctgggg aggactggca 660 gacaaagtgg gaaggaaaca gtctcttctg atttgcatgt ctgtcaacgg attctttgcc 720 ttcctttctt catttgtcca aggttatggc ttctttctct tctgtcgctt actttctgga 780 ttcgggattg gaggagccat acccactgtg ttctcgtact ttgctgaagt cctggcccgg 840 gaaaagcggg gcgaacactt gagctggctc tgcatgttct ggatgatcgg tggcatctac 900 gcctctgcca tggcctgggc catcatcccg cactacgggt ggagcttcag catgggatcg 960 gcctaccagt ttcacagttg gcgtgtgttt gtcatcgtct gtgcactccc ctgtgtctcc 1020 tccgtggtgg ccctcacatt catgcctgaa agcccacgat tcttgttgga ggttggaaaa 1080 catgatgaag cttggatgat tctgaagtta attcatgaca ccaacatgag agcccggggt 1140 cagcctgaga aggtcttcac ggtaaacaaa ataaaaactc ctaaacaaat agatgagctg 1200 attgaaattg agagtgacac aggaacatgg tataggaggt gttttgttcg gatccgcatc 1260 gagctgtacg gaatttggtt gacttttatg agatgtttca actacccagt cagggataat 1320 acaataaagc ttacaattgt ttggttcacc ctgtcctttg ggtactatgg attatccgtt 1380 tggttccctg atgtcattaa acctctgcag tccgatgaat atgcattgct aaccagaaat 1440 gtggagagag ataaatatgc aaatttcact attaacttta caatggaaaa tcagattcat 1500 actggaatgg aatacgacaa tggcagattc ataggggcca agttcaaatc tgtaactttc 1560 aaagactctg tttttaagtc ctgcaccttt gaggatgtaa cttcagtgaa cacctacttc 1620 aagaactgca catttattga cactgttttt gacaacacag attttgagcc atataaattc 1680 attgacagtg aatttaaaaa ctgctcgttt tttcacaaca agacgggatg tcagattacc 1740 tttgatgatg actatagtgc ctactggatt tattttgtca actttctggg gacattggca 1800 gtattgccag ggaacattgt gtctgctctg ctgatggaca gaattgggcg cttaacaatg 1860 ctaggtggct ctatggtgct ttcggggatc agctgtttct tcctttggtt cggcaccagt 1920 gaatccatga tgataggcat gctgtgtctg tacaatggat tgaccatctc agcctggaac 1980 tctcttgacg tggtcactgt ggaactgtac cccacagacc ggagggcaac aggctttggc 2040 ttcttaaatg cgctatgcaa ggcagcagcc gtcctgggaa acttaatatt tggctctctg 2100 gtcagcatca ccaaatcaat ccccatcctg ctggcttcta ctgtgctcgt gtgtggagga 2160 ctcgttgggc tgtgcctgcc tgacacacga acccaggttc tgatgtaata 2210 2 727 PRT Human 2 Met Glu Asp Ser Tyr Lys Asp Arg Thr Ser Leu Met Lys Gly Ala Lys 1 5 10 15 Asp Ile Ala Arg Glu Val Lys Lys Gln Thr Val Lys Lys Val Asn Gln 20 25 30 Ala Val Asp Arg Ala Gln Asp Glu Tyr Thr Gln Arg Ser Tyr Ser Arg 35 40 45 Phe Gln Asp Glu Glu Asp Asp Asp Asp Tyr Tyr Pro Ala Gly Glu Thr 50 55 60 Tyr Asn Gly Glu Ala Asn Asp Asp Glu Gly Ser Ser Glu Ala Thr Glu 65 70 75 80 Gly His Asp Glu Asp Asp Glu Ile Tyr Glu Gly Glu Tyr Gln Gly Ile 85 90 95 Pro Ser Met Asn Gln Ala Lys Asp Ser Ile Val Ser Val Gly Gln Pro 100 105 110 Lys Gly Asp Glu Tyr Lys Asp Arg Arg Glu Leu Glu Ser Glu Arg Arg 115 120 125 Ala Asp Glu Glu Glu Leu Ala Gln Gln Tyr Glu Leu Ile Ile Gln Glu 130 135 140 Cys Gly His Gly Arg Phe Gln Trp Ala Pro Phe Phe Val Leu Gly Met 145 150 155 160 Ala Leu Met Ala Asp Gly Val Glu Val Phe Val Val Gly Phe Val Leu 165 170 175 Pro Ser Ala Glu Thr Asp Leu Cys Ile Pro Asn Ser Gly Ser Gly Trp 180 185 190 Leu Gly Ser Ile Val Tyr Leu Gly Met Met Val Gly Ala Phe Phe Trp 195 200 205 Gly Gly Leu Ala Asp Lys Val Gly Arg Lys Gln Ser Leu Leu Ile Cys 210 215 220 Met Ser Val Asn Gly Phe Phe Ala Phe Leu Ser Ser Phe Val Gln Gly 225 230 235 240 Tyr Gly Phe Phe Leu Phe Cys Arg Leu Leu Ser Gly Phe Gly Ile Gly 245 250 255 Gly Ala Ile Pro Thr Val Phe Ser Tyr Phe Ala Glu Val Leu Ala Arg 260 265 270 Glu Lys Arg Gly Glu His Leu Ser Trp Leu Cys Met Phe Trp Met Ile 275 280 285 Gly Gly Ile Tyr Ala Ser Ala Met Ala Trp Ala Ile Ile Pro His Tyr 290 295 300 Gly Trp Ser Phe Ser Met Gly Ser Ala Tyr Gln Phe His Ser Trp Arg 305 310 315 320 Val Phe Val Ile Val Cys Ala Leu Pro Cys Val Ser Ser Val Val Ala 325 330 335 Leu Thr Phe Met Pro Glu Ser Pro Arg Phe Leu Leu Glu Val Gly Lys 340 345 350 His Asp Glu Ala Trp Met Ile Leu Lys Leu Ile His Asp Thr Asn Met 355 360 365 Arg Ala Arg Gly Gln Pro Glu Lys Val Phe Thr Val Asn Lys Ile Lys 370 375 380 Thr Pro Lys Gln Ile Asp Glu Leu Ile Glu Ile Glu Ser Asp Thr Gly 385 390 395 400 Thr Trp Tyr Arg Arg Cys Phe Val Arg Ile Arg Ile Glu Leu Tyr Gly 405 410 415 Ile Trp Leu Thr Phe Met Arg Cys Phe Asn Tyr Pro Val Arg Asp Asn 420 425 430 Thr Ile Lys Leu Thr Ile Val Trp Phe Thr Leu Ser Phe Gly Tyr Tyr 435 440 445 Gly Leu Ser Val Trp Phe Pro Asp Val Ile Lys Pro Leu Gln Ser Asp 450 455 460 Glu Tyr Ala Leu Leu Thr Arg Asn Val Glu Arg Asp Lys Tyr Ala Asn 465 470 475 480 Phe Thr Ile Asn Phe Thr Met Glu Asn Gln Ile His Thr Gly Met Glu 485 490 495 Tyr Asp Asn Gly Arg Phe Ile Gly Ala Lys Phe Lys Ser Val Thr Phe 500 505 510 Lys Asp Ser Val Phe Lys Ser Cys Thr Phe Glu Asp Val Thr Ser Val 515 520 525 Asn Thr Tyr Phe Lys Asn Cys Thr Phe Ile Asp Thr Val Phe Asp Asn 530 535 540 Thr Asp Phe Glu Pro Tyr Lys Phe Ile Asp Ser Glu Phe Lys Asn Cys 545 550 555 560 Ser Phe Phe His Asn Lys Thr Gly Cys Gln Ile Thr Phe Asp Asp Asp 565 570 575 Tyr Ser Ala Tyr Trp Ile Tyr Phe Val Asn Phe Leu Gly Thr Leu Ala 580 585 590 Val Leu Pro Gly Asn Ile Val Ser Ala Leu Leu Met Asp Arg Ile Gly 595 600 605 Arg Leu Thr Met Leu Gly Gly Ser Met Val Leu Ser Gly Ile Ser Cys 610 615 620 Phe Phe Leu Trp Phe Gly Thr Ser Glu Ser Met Met Ile Gly Met Leu 625 630 635 640 Cys Leu Tyr Asn Gly Leu Thr Ile Ser Ala Trp Asn Ser Leu Asp Val 645 650 655 Val Thr Val Glu Leu Tyr Pro Thr Asp Arg Arg Ala Thr Gly Phe Gly 660 665 670 Phe Leu Asn Ala Leu Cys Lys Ala Ala Ala Val Leu Gly Asn Leu Ile 675 680 685 Phe Gly Ser Leu Val Ser Ile Thr Lys Ser Ile Pro Ile Leu Leu Ala 690 695 700 Ser Thr Val Leu Val Cys Gly Gly Leu Val Gly Leu Cys Leu Pro Asp 705 710 715 720 Thr Arg Thr Gln Val Leu Met 725 3 197997 DNA Human misc_feature (1)...(197997) n = A,T,C or G 3 gcagaagctt tccattatga aaggcactgg ggtagtgctg tagggtcatt cagactctgc 60 tttcaactaa ccagaatttg gagctcactt aatggtattt ctggagctga ttatgtacag 120 gaagcagctg atatgcagtg tctgatgtgg tcatccgttc aatggatagg atggcagttt 180 attttattta ttgtgtgaaa tattcagatt ttctgttaat ttttaaagta ggaaatgtct 240 ctcaacttca tcattgtcct ctctgcccct tttccctgac ttcttgagtt tttgagcacc 300 ccatcctcag cctccctttt caccaccttt ccattttttc ttactgttct catggatttt 360 cccatgtctc tcttcctccc tttctcttcc tctcagttct taaaaaaaaa aaaaaaaaaa 420 aaaaaaaaaa gagctggggg agaacaaaca gaaaaatcat ttactaaaac atttggttaa 480 aaaccttgca atcatatgtt tattttaatt tcattttatc attttccaaa acgcagcctg 540 tgagaaccat actcctgttt tgcctactgg gcatttgtag gtattcagtg ggaatgaagc 600 atggtccatt tataaacaaa catctgtttg tctttattgc tgggtttgtt tgtggcattt 660 ggaggagtaa ttaaatgtat gttagtaata gctactttaa atattttaaa ctagctacaa 720 aatgaaaaag tagaaatgac gattatataa atcaatgttt ggaatgctgc catagcttaa 780 ggcttgggga gttaatgatg ggggagaaag acttttttaa cctctgttat catctgtgta 840 caaggatgaa cctgtcagtg actcacatga gccaccagaa gaggcagctg agtggacaaa 900 tggaatataa gttaccttga ggtcagcctg aatttaagga accaaggcat gaggggacta 960 tcagtgaaaa agaaaaccaa ataacaggtc ctggaaggtc cagactttac taccaaaaca 1020 gcagctaacc agagtggcag gtgggaggat gtgagaggga gagagggaga gagagagagt 1080 gtgtgtgcgt actgggtatc cagagatgaa gaaaatagaa gtggggttgt gaagggacat 1140 ggcatggagc ttacctgtcc ctgaaggcct gggggtagtt tctgaaaggg cagactccca 1200 ccaaacaagc catatgttct aattcaatat caaatgcagc cataaaatca tttgatgaac 1260 tctgacaatc ctgtggatat gacactatgc tatgggaata tactccttgc cagtttgggg 1320 tgccatgtga gtctctgtta ccatttgttg gaaggaaaca caatgttaag taggggagaa 1380 attcaatttt acaaatatag ctatagtttg attgatagca attgtatatg gggctgcttg 1440 cttttcagtg tgaggaaata ggcccagtag gattcttcta atactctggt ttgtctttgc 1500 aatatttgta cgtgtctgtg agcaatacta tttttctcaa tcaaggtata tgcttgtagc 1560 atttcaaaat gtgttgggcg tgcttttaaa gatgcaagat gctctgtttt attctcaggg 1620 gaattcctgt gtactgttac cctggggata gaattcctta aaataaaaca ataataatag 1680 cagcgaaaac cctaaatact ccaagtgatg ctcacactta agcagagaag cagcctaggg 1740 tgtacatgta tatacagtga gatcaaaaaa aaaaaaaaaa ggaaaaacta attctgtgtt 1800 gtagtttgtg ggacactgaa aagaggtcaa gaaatagacg gtaaaaaata tatatataga 1860 aaggaataat aagtatctcc tctatttctt ctttcgcagt tctgttttga acccaaagtg 1920 gatgatgctg tcagagctga actactgaaa ggaggctgtg aaaatttccc atcttctcat 1980 tggccatcag ttgagataag atggaagact cttacaagga taggacttca ctgatgaagg 2040 gtgccaagga cattgccaga gaggtgaaga aacaaacagt aaagaaggtg aatcaagctg 2100 tggaccgagc ccaggatgaa tacacccaga ggtcctacag tcggttccaa gatgaagaag 2160 atgatgatga ctactacccg gctggagaaa cctataatgg tgaggccaac gatgacgaag 2220 gctcaagtga agccactgag gggcatgatg aagatgatga gatctatgag ggggagtatc 2280 agggcatccc cagtatgaac caagcgaagg acagcatcgt gtcagtgggg cagcccaagg 2340 gcgatgagta caaggaccga cgggagctgg aatcagaaag gagagctgac gaggaagagt 2400 tagcccagca gtatgagctg ataatccaag aatgcggtca tggtcgtttt cagtgggccc 2460 ttttcttcgt cctgggcatg gctcttatgg cagacggtgt agaggtgttt gtcgttggct 2520 tcgtgttacc cagtgctgag acagacctct gcatcccaaa ttcaggatct ggatggctag 2580 gtgagtgtgt ggtgtcagtg aggccaactc tgaaactggc ttatttgtta agcacagtta 2640 tcaacataga gaataaatac ttttcatcta gagtactgca ttttttctaa caaatttttt 2700 attacaaaaa actttaagca tacaaaagaa tcaaaataat tgtacagtga acacctgtgt 2760 atacccacca cctagattct gtagttaaca tttactatat tttctttatc atctctccat 2820 caacccaatt tatttttatg catttcaaag taagttagag tatgttttaa cttatatttt 2880 tatcatagtt gcattggttt ctcctttatt aaattccaaa gctactctct tggaagttaa 2940 taatgctgtt atatttaaca cttttctaaa tacagagatt agttctgtaa aactctgaca 3000 attgtgattt caaatgtcaa tttttatgtg taatacagta gtttaaatat tttaggaccc 3060 tgataacttt aattttttct attatctatg tgtatgtttg cttagatttt caactagcct 3120 tattcgtata attttctata ggtgattttt aatctttttc aagctgtgta actttgtgcc 3180 caaactagaa aaacttcatt tctttatatt attttaaata tataaatatt taaatgtgtg 3240 ttaaaaataa tataacctct ctataaaata tctatacagc agatttgatt tttgttttga 3300 agttaccatg gactcaggtt tatattttta caaagctata gttaacactc tccgagtttg 3360 tgcaagggaa aataagtagg taagaagcaa gcatgcagtg aactctcccc tcagcagttt 3420 aaggatccac agcaaacact tggtatataa aatcaggagg atttccaaac tgcttttgca 3480 aatcctgcct cagagaacac gtttatgtgc atgttaaatc tttatgtgtt tcagagtcta 3540 tgaaagaaag aatacttagt atctatgttt ccaagggact tctttaaaac taaagcttca 3600 aaaacaaaag tatacaattc ttgaaaacta gtaactttat taagtaatgt tattatcatt 3660 gaggctgaga aatttcctgg gtgattaaaa ttaaatgcta tatcctaatt tgttctgtga 3720 caacacaaga gagatactca tgaatgagaa aatgatatac atctttttat tcctttaaca 3780 cttgtttctt ttatatgacc cactcctagt atttgtttct ttatgcttag ctaaaataaa 3840 tggatccatg aaagtagatt gtgtaggttg aaggggtggg agtcaggagc atattgtgca 3900 tattttgctg gtacactcat ttgtgtgttc cactatcaaa tgcagacatc accacgcttc 3960 agaggagggt ccttggtcat ttgtcaaatt gatcatctca gcagactggt tttgtatcta 4020 gcactgtata tcatttttgt tttttttctt ttggtgagca agtatatcca actgtaagcc 4080 tagacatcaa aatatgtatt cagattttgc taaaggaata tgtcagcaga atgtgtaaag 4140 aagatagacc ctgagaaata tactcacaca aatatttaat aattgccagc ttaaatttag 4200 aataaaaaat attttaatgt tatggtccca ccatttatac tggaaagact acagaaaaat 4260 ctaagccaaa cttgccctat gcctttatct tcctggtgct gtatgaaagc acagtatgac 4320 atacggttct cttatcaaac tatcctccaa acccagaaac cctggtgatg ttacccaccc 4380 ccaggtggca tttagaagtg tagataatgc tgcagaacaa ttggagcaca gatattctca 4440 tctgcccata aatgttgcct gctttagtga attaaaaata tgtccccagg agtgtatagt 4500 ttcactcagt gttgtgagtc cccaacagga gcattttaat gctttcataa ctgtgccttc 4560 cagacttgca tttcaattat tttgccattg taatttcact cataattgca ttctaacaga 4620 gtctttttag acaagcattt aaattgtttt gccattttca tttcatcgaa taatagcttt 4680 gtaatataat taccacacta agtcatagca ataggatcaa aatgcaagaa agcaggatgc 4740 atgacccatg tttggtggca ttttgaggat atagatttgc aaaataattt ctcatttgct 4800 actaagcatt gtgtcatttt cctcaaggaa gaccaggaac aaaaatatgg cccaatttat 4860 gttttaatta tttgggagat tgagcctctc tttgtgtttg ctaaaagatt gacttaaagg 4920 tatctaaggg catacagtgt atgcctgtat ctgtgtatat agtctgtgtg tatatataac 4980 attgttaatt gggccagaag aagttagagt aaatatttaa ttatctaatg atctgctctt 5040 tggaagaaca agggcagtca ctttaagata atctgttctc tattgccaaa gtctatttat 5100 taagcactta ctgtgtgtaa agcacagtat tgtcacaagg ataataggac aaaaaagaga 5160 ggcaaggttt ctctatccaa ggaagttata gtctagtaat ggagaaatca caaatgagta 5220 gattaccagt ctagatacca agtgtcagta gccataagac aagtgtaaat aaagagactt 5280 cgagcaggga gaacatgtta cagggtaagg ccctgactaa agcagtggag agaaggaatg 5340 gatttcagag ctactccaga ggtagagtca gcaaggattt gctcattgat tagcttcgag 5400 tggggaggag gggaccaatt aatgaaagaa tggcagttca ttaaattagg taaggcagaa 5460 tatagatttt agcaggatgg agaaagatga aaatgtttag tttgagaccc tctcattttg 5520 aggtccttgg ggacaatcaa agaggagtgc caatgggcta ttccccattt gagctgaaat 5580 gcccagagat ttggaagtca tcttgtttaa gacaacactg ccaatgacct taatgaggga 5640 gagaatggtt tcccgctgaa tactatttat ggggctggtg gtggaggagg aatcattaag 5700 gaggtagagg agaagccaga gaaaggagca gccaggagaa aggggaggtc tggaacttgc 5760 tggaagaagg aagggatgta tagctttcag gcgccgcagt cttgcagtag aggctggcaa 5820 ttaagagtta ttggctgctg cagacatctc ttaagatgaa cacagtgagc acaataaaca 5880 caatgagcca gactagtaag tttttaagac ttcttattga atgaagtgaa aatgtgtcct 5940 gctgatttcc agcccctggt cacaaggcag gcttttccag gacatcaggc attctccctt 6000 cccatttgct gtgactcaga gtggattctg tggaagtgag ccctctgact tagcttctgt 6060 tcagtggttg ttgagggggg caggtatggt tctaagatta cccctaggca ggaaagatgt 6120 gcaaccattt tcagctcagc cctaccccga ctctagtcca ttagggcaga acaagcagcg 6180 tgctggcacc tcttcccttg catcagaggg gctggcctcc cactcactgg cttatttcct 6240 cctaagaccc atccagtgaa gcagagaaaa tgatcattat tggttcaact gctttattgc 6300 tgagggttac aaagatgagt gatatttagg aactaaaata ttatcacagc caggtgttga 6360 ctaatacaaa atagatgcaa agcaacatat tctaacccca aatgtacttt tataactaaa 6420 cctgagctaa ttaatagtga aacatcctgt gcttctttta tccttttcaa ggcaaatatc 6480 aactgctacc cctaattccc gtatgcattg gtagttttca ggacaggagt tttagcagca 6540 taattgagac agggccagat tgctttggga agaaacatga atgttgttga gaaaaatgga 6600 gacagcaaga acatttcatt tgataagcat taattgagta ccaactctga gccaggtacc 6660 tggcaaagta cattgagagg atattctgat tagtatattg tgatctgtgt cattcaggaa 6720 cttttttgtt gttgttacaa tgtgacaaat acacttgtga taaattttag tgctttatgt 6780 tttttttttt ctgtgacaaa gatactgaat aaaatgctat aggattgcag aagagggaac 6840 taccaggctt acctttggaa actgatgaaa actcatctag gaattgacat ttaaacagag 6900 ttttgaacca tggcacgtgt atacctatgt aacaaacctt cacattctgc acatgtatcc 6960 cagaacttaa aataaaaaat ataaacaggg ttttgagagc tggacaagag tttaccgggc 7020 agataaaagg aaggatggca cttcgggaag atgttataat tgcccaatag cctggacttg 7080 gtaggcaatt atgtgaactt atctgtggta ggcgtagtag tgttggagct gacaggtcac 7140 attgtggata tattgtgaag accttaatgc caagataggg gtttggactt tatcatgaaa 7200 tcattagacc tgtgttttag aaagctgcct ctggcagcag tgtggagaga gaatgagatg 7260 ggagagagac ttaaaatgtc aaatagactt taagccactt acttgtgtga ccatttgcaa 7320 gtaatttaaa tttctctgtg cctcggtttt catagctgta cgttggcaat aagaataccc 7380 aattagattg ggaggattga atgagataat atatgtataa ctcttagcaa agcattgaat 7440 atatagcaaa cactaataaa tgatagctgc tataataatt aatagaaggg tgaatatcag 7500 ttaggaggct aatgcaatag cccaggcaaa acatgaagaa atccctaact gtatcagtta 7560 atagggtgac agaggtgtca gaatcggaag catttctgga agagaaatgg aaaaatttgg 7620 tgaatgagaa agataagcag gtagttgagg atgatagcca aggctttggg caggtacagg 7680 gaaggcagag cagatgggga ggctgatgaa gttcattttg gagggtgtta gtctggtagg 7740 tagaagaggt caggaccaga atggcagatg tgagattctg aaaagggagg tttaaccaga 7800 gaacacagct aaacaggagg cggacaatga ctaatgaagg tatcagacag cttagcaagg 7860 ggttagagag tctttcggcc ccaaaataac taacagccag aagtaaattc ctctgcatga 7920 cacttttata ttttttaatt aaaaaaatca tattacaatc ctcttaaatc tcctgtttcc 7980 cttcagaacc agtagccatg tttaaaattt ggtctatgtg cttctctaga catgccaatt 8040 agatgtgctt gttctacaga gccctctttg aagctggagt gggagtgggg aggagggttg 8100 acaggacccc ttctggcatc cctttcctct tcttcatcag agcagactca gattcatcca 8160 tttaggtgag aaaggattcc atagctaaac aacaaaaaaa ttttaatatc ttatctggaa 8220 tatcagtgac ttcagctgtc acactagtgt tacaggttca gtgtttgatg aagtggtgtt 8280 atgaagtagc caggatgtgt gggtcatggc ccaaggccct gaaagagaga ttgagatgga 8340 taaggtttgg ctcctattac cagcgggaat cataatattt cccagctgtt gtttcctttg 8400 tgatttaggc aaaccctgtc attttaaaga tgaagatagg agacctagag agctcggttg 8460 gcttgcctga tctgtagctg ggtattagct gaggcagact ctggaacaca tataccaact 8520 cttagtccta tgttcctttc cagttccctc acgttttcat ttaaagcact ttcaatgggg 8580 ataaccctga gaagaacaca aggctctcca ttcttggtga aaatacacca ctctggtatg 8640 tcaagcaaga gaaagatgat ctggaaattc actttttgct tgtgaagtcc aacagagtaa 8700 tcacctgttg aacttttcaa aaaccttaat agaagatatc acctgttttt tgtaattata 8760 aacagtacat aatggcaaat atatggtcac aaatgagaaa tttgattgcc tcagagaatt 8820 aacctatcaa attagcccat agtgcaaagt catcttaaag aggcagagtg acagaaaaaa 8880 taaagaaagg gaatgtaagc ctcatgggaa ccagggattt tgtctgttgt tccctactgt 8940 atcctcctgc ccagatcagt gtgtggtaca taataggatc tcaacaaata tttgttgaat 9000 gaatgaatga cagtctctga gatctttaac actttgagaa ctaactcaag atatgaacat 9060 acctaaaata agaatgtact attttgggcc gggcacagcg gctcacgcct gtaatcccag 9120 cactttggga ggctgaggca ggcagatcac gaagtcagga gatttagacc atcctgacta 9180 acacagtgaa accccatctc tactaaaaat ccaaaaaaat tagtcgggtg tggtagcggg 9240 tgcctgtagt cccagctact cgggaggctg aggcaggaga atggcatgaa cccgggaggc 9300 agaggttgca gtgagctgag attgtgccac tgcactccag cctgggcgat ggagcgagac 9360 tccatctcaa aaaaaaaaaa gaatgtatta ttttgaattg aaactcagta gaggaggaat 9420 accaaagaga aagtcataga tgcagatgtt ggcagtgccc ctccaaatag tgctcagggt 9480 tgaggtgccc tgacctaaag atttagccta tctatctagg ggccacatgt atctctattg 9540 tagggaaaga tgcattagac atccttggcc tgggatttgt gaagacacga atgctgtagc 9600 taagcacctc tcattctcat tacatgttcc aaagcaactt aaggatttca aattcagctt 9660 tctctagagc ttgcccatca gaaatatgct ggccaaagat tgccagtttt cttgggagtc 9720 ctgtttttca ttttaattgg ccaactggca ttctgttttt ttctatcagt cgtggtgagg 9780 ttgaccattg ggagactgct tctaagagat agatctgtct ttagtgtgct gacatatcgg 9840 gtttctgaac atataagact gaggtccaaa ccagtcttgc atcctgggag acctacttgg 9900 gtgttcacct agtttcctgg agaaatattc caacttcata gatactaggt tttctttaaa 9960 acatctgcaa tattagctag agtcaattgg tatactggac gaaaacagag ctagaccaat 10020 aatatcaaag cattaaaaag acaatacttt tacatataac tggaatttta cttgactttt 10080 ttccattttt agaagctctt gaaagtattt tttttttttt tttttttttt gagacagagt 10140 ctcactctgt cgcccaggcc ggactgcgga ctgcagtggc gcaatctcgg ctcactgcaa 10200 gctccgtaga agctcttgaa agtttttaaa atatttctaa ataattctag aataaatata 10260 gatatttaca aatctgggat atatttttgg atagaaatgg agtagttgtt tttgttttac 10320 ttaaaacttt cctatcaact gtaccaagtc agactacttg gcattcctat atcttccatt 10380 gatagctaga gtgttcactc aaccaaaatt tattgagcat ctactatgtg acaagcacta 10440 tttggactct agaaatataa tagtaataaa acagaaaata tctctggtag atcttgagca 10500 tgttttcagc ttctctttct gtagttttta cttggatgct ggaattcctt gaaatataca 10560 gaattcattc ctcccatgcc cctttgcttt gtttgacctt ctaggaaggg tagtttcttt 10620 gagagaagag ctggatagat atgacctgaa acccttattc ctttagggat taacaaaagg 10680 ataaatattc tgttgggatt tctagctgct ttattgagag atagctgggt ctgaaaacaa 10740 cagcttcaag tttctaagat tcccctttgc tagtttcata gactcttatt ttccactaga 10800 ctgcaggtgg agtatagtga ctggctcacg gtgcattgaa ccaatctttt tagagtgtgt 10860 ctaacccctg ctcagtgctg ccaggtagac cagcatatcc aagctgatga catagaggga 10920 cacagagttt ttcctctttt agctttattc caaatttgtg atgatttggc atctgagtgt 10980 ggcctaaatt ttaagaccca taactttttt tccctacaag tgtacactgc agactttagg 11040 atcagcagct ctataccctg cttgatctct ttgtcctcag catctatgtg ggtacctctc 11100 atggaacttt attctttgtt gtgtatgcac cctctgtctc tgtttagcct ctcaggcatc 11160 acagtgatcc taattggagt taaaaagtta ttggctcata gagcacatcc tgtctaccaa 11220 cactagcaaa atcttatttg gattcattat tgcaaaattg gaaaagaaga ttcaataatt 11280 tatcgccatt atggtcctgg atcttatttc ctcttgcctt tcaaattctc ctgggaggag 11340 agaggcttct gacatccgag tggaggtgga ctgagcagcc tgaaaagact cttccatcca 11400 aagagtcggg ttcactttca caggacagga aatgaacaga aactctctgc ctcattcctg 11460 aaacttgcca ataagtagca ttctaagacg gtgagaattt gataggaaaa tatccttctg 11520 ccaccctgaa ggtcactgag ggttttccat aatacagctc tgaacctgaa ggcagatttc 11580 tctatttcta cactctgaat cattgtatcc ctatatccta gaacactcta ggatgaggat 11640 tctgatagga atcctcacta acgctgagag cactggcctt acatacattg atccagtctg 11700 agactctacc taaagtcact ggagaacttg ccgatgtatt tcaggttgga gaatttgttc 11760 atgtggactt cactttaaat aaggcaattg aagccaggcg cggtggctca cacctgtaat 11820 cccagccctt tgggaggcca aggcaggtgg atcacttgag gtcaggagct caagaccagc 11880 ctggccaaca tggtgaaacc ccatctctac taaaaataca aaaattagcc aggcgtggtg 11940 gtatgtgcct gtagtcccag ctactcagga ggctgaggca ggagaatggc ttgaacctgg 12000 gaggtggagg ctgcagtgag ccaagatcac gccactgcac tccagcctgg gtgacagagc 12060 aaaagtccat ctcaaaaaaa aaaaaaaaaa aaagtaaaat ttaaaatatt aaaaaattaa 12120 aaataaataa ggcagttgaa ttgttaatta tctaacaatt ggatcaagcc aaatataata 12180 atacagactc ataggtttga tttcagatta tcagtatgca gttgcaccct ttcatgtgaa 12240 atctaacatc caagtattct cacataaaca agagcaaggg cctttaaatg gcctgagatt 12300 actgctgaac acttcagtgt aagaaactgt tcatcagatt catcttaatg aattagtctg 12360 ataaattact gattaagtat ttctaacaca catatttgca aaccaacatt tttaccctaa 12420 atttagaaat ttctttttgg cctgtaagat tgtgagagaa aaagtaaaag aagtaaatga 12480 gtgtagttct tacctttaat ttcaaaattg aggtctctaa tcaaattgaa aatgatgagg 12540 aaacaatgag tttttccaat taaaaatttt tttactgtaa ggaatttgct taaaaataga 12600 atttaatgat ctttaaatta ttttcacatc tctctgtctt gattctgttt aatgtgctta 12660 taatcataac tgcaattatg taaatacaga ttagttcatc tttgctaact tcacattatt 12720 tcacataaag attttggaga atcaatacag tgtgttgtgg gcatcagtga gtatatggtc 12780 attcataatt cactgttcca gtcccttgct tagccatctg ttactgagca tttgaatgta 12840 tccagttctt tgcttattat atgaaaataa tgctccaatt aatgtcatta ctaaaattac 12900 tcttttgttt gggacattta gttggcatgt tttccccaaa gcaggagtga aagttactaa 12960 tgagtatatg atattttaat ttctttttta aaaacattaa cataagtaat actgaaagaa 13020 gaaaataata tggcaaatat tcaataattt tggaaatgcc atacaacaca ggcccaggga 13080 acagtcaagg ctgactagga agtcctttgc atattctcca aatatggtat ctcttcaact 13140 ttttcttttc cttttttttt tttttttttt ttttttgaga cggagtcttg ctctgttgcc 13200 aggctagagt gcagtggtgc aatctcggct cactactgca acctctgact ccctggttca 13260 agcaattctc ctgcctcagc ctcccaagta gctggggtta caggcacctg ccaccacacc 13320 cggctaattt ttgtattttt agtagaatgg gatttcacca tgttggccag gatggtctcg 13380 atatcctgac ctcatgatcc acctgccttg gcctcccaaa gtgctgggat tacaggtgtg 13440 agccaccgcg cctggctggc ctcttcaatt ttttcttaag gaaattggtt gcaatgatga 13500 tgatgaaaag gcattagtgt gcccagggga aaacattagt ggtcagatga tgagacacag 13560 agagacgata aaggtgtccc atcttaggta cttaccctcc aaacactgaa gtagccccta 13620 atcccttacc ccagaaacga gttcattctc ccacgtccaa atctatgtta gcatttctca 13680 accggagccc tagtaacagg gacaggtctg tgctgtgtga cactgtctca cacattgcag 13740 gatgtttact agctgtggcc tctgtccaca aaatgtgagc agtgcttccc aggcattcct 13800 taaaacatgt gccttaccac actccagaca aggcctctca aatacatatt tctacctgac 13860 ccccaggaag caataggatc caggactcag gctctggtgg tggtattacc acaacagcag 13920 tggagactgg agttcagtgt gggggaaaca gaagagttgt ctcttgtgct ggctttctgc 13980 cttcctagta gtggttctac atcttctagg attgtggttt taacatcgag gatcttctac 14040 tgatcttgac aatggtcttg aattttttca aactaacgaa tatgtattag aaaatcaagc 14100 tggtcatcaa ctgagccgtt cttggagttg tagagcttag agaggaaagg tgatttagtg 14160 ctcacagaat caaaaaccct aaatttgaag aagagaaaaa ctgaagtcaa ataaagagga 14220 aatgattcac atagaggcac atgccattac cagtgaggta ggaataaaaa cttgatggcc 14280 taattttcag actgggattt tcttcatcca aaaagtgcac aatctcagtt ggtggtggtg 14340 gttacgcaac aatgtgaatg tactcaacat cacatcatag taagatgata aattttatgt 14400 tctgtgtgtt tgatacaatt taaaaaatgc agtgttccta tgcatttagg aatgaagagc 14460 tatttatcaa tattggaata ttgcttattt ctgcaataaa atttattttt tcactgtaaa 14520 gaccgagcct ggaaacagtg aagctgacag ctgagaaact gctgaaaacc ctaacgaaag 14580 cactttccag atccaactta ccttgtgggg ctgccatgta aaattgtgca ggttgtgacc 14640 tgcacaaaag gacctgggtt tgtggaaggg gtggggttgg aatccagccc tttcactcac 14700 cattcatctt ggtttagggc tgcttttatt tatctggagg aaaggatgat ctttgctaat 14760 tcctcttcct tgaaggagct cctttacgta attcatccac ccagatgaat ctgccttttc 14820 ccaattcatg tagaagtgtc ttctgtgcca ggaggctctg ttacttgatg atcaacttta 14880 gacagtgggg aacctgagga gatacaaaag gagaagggat ataagaaacc atgaccttac 14940 ccttccaaga actgagggca gatgcagaat attcagaaga tgagaacctt ttaaagggaa 15000 aatgtaacta aagtgcaaat ataattcagt aaatattcat gtaagcagtt acagttctgg 15060 attttaagta cagtcagatt gataggaggt ttttttttgt tttttgtttt tttagcaaat 15120 catgtgtcta attaacttaa aatatttggg aaattctgag aaatgaggga attttgagtt 15180 tataagtaca ctgcatgtta atgacccaga ataatagaat tggagagaat ggaccatctt 15240 ctctagctga ggcccaggcc caagagaaga gctcacacag agcagaacta agctacagcc 15300 accagggatt ttgtggggaa gattacctcc atactgggag gggcccatgc aatttgcatc 15360 accatagctg ccctctccag gagttcttgg gagagcaatt ctgtttaggt acatagcaga 15420 agagcaagct cctagagtgg aagttaaagg ggctgcttaa tgtttggtgt gtactttgtg 15480 aggttaggga ggtaagatgt taaagtcaga tgctgctcta cttagtatgg gatgtcaata 15540 aggtacttac ttcaaagtag gtactggaac atacctttct tctaagagac taagattttg 15600 ttgttgttct tcagagaagc atttgtggaa tatttctgta gtgcttgaat ggccaaagat 15660 caataaacaa aataaattac agtcctggtg gattttaata tttaaattta gataattatg 15720 atgtccaaag agaagagaaa gagatgcttt aagtcattac caaaaatatc ttagtccttg 15780 agagggattt gctgaaccaa agtgagaatg aagtctgtgt gtcccctttt ctgcaaagaa 15840 gctgacgcca catgatgcaa aggcctgccc ttaacccagt taatatgaac atggcatggc 15900 accaaagagg agacagaatc gacagggaga gtcccagttt taagctccca agcaagatct 15960 ttgagtgggg atgggagggg tccttttacc ctttcctaat gactaaatta aaccaaatct 16020 acccaagtgg cccctcctct cctcctggga gatggtggtg ccattgtcaa gataggtggg 16080 acacatttgg gagagattag gggaacacag tttcaccttc acatttaaga atccaattta 16140 ctcctgtggc cttgtttgaa ctctccttcc aacactcata taataataat tatatgtcct 16200 tgccttctgt aagctcattt cagttggtgt agcaaatata tttctaccac acacacaaaa 16260 attcttctct taggggttca tgtggaaatg aaaacacaat gtgttccact ccacgtgaaa 16320 agcaaggctg ctgtctctgc agcaaagtgc ttcctgacaa ggactgagct gaagtgtcat 16380 gaagctactc acctggaggg ggcatcagca tctcctgcct cttaagtcga ccagggaaag 16440 cttggacaag agcagccatg taggtgttac aggaaaggtc ctgatccaga cccctagaga 16500 acgttcttgg atgtcgcgca agaaagaatt cagggtgact ccacggtaaa agcaagttta 16560 ttaagaaaga aaaggaataa aagaatagct actccatgga ccagccccga gggctgctgg 16620 ttgcccattt ttatggttat ttcttggtga tatgctaaac aagggatgga ttactcatgc 16680 ctcccctttc tagaccatat agggaaactt cctgacattg ccatggcctt tgtaaactgt 16740 cagggtgctg gtgggagtgt agcagtgagg atgacctgag gtcactctcg tcaccatctt 16800 ggttttggtg gcttttggcc agcttcttta ctgcaacctg ttttatcagc aaggtcttta 16860 tgacctgtat tttgtacaaa cctcctatct tatcctgtta cttagaatgc cttaaccttc 16920 tgggaatgca gcccagtagg tctcagcctc attttaccca gctcctattc aagaccgagt 16980 tgctctggtt caaatgtctc tgacatgggg gctaatgaca tcagaactgg aaagaaagat 17040 gataaaggaa tgagtttttt taaaaaaaaa aaaaaaaaaa aaaaaaaacc aaacaaaaca 17100 aggataccaa atggaaaata aacaaaaaaa aagttttaaa aagaaaaaaa ttaagtgctg 17160 actgtgctac aacatgcatg aacctgaaaa acatgatatt atgccacaca caaagggaca 17220 aatattgcct atgaattatc tagaataggc aaactcatgg agatggggaa tggattagaa 17280 ttatgaggga ctcttttaga attatccaga gcaccatcca agggagaggg gaatgggaag 17340 gtacaatgtg tacagctagc tataggtttg tggtgatgac gaagttttgg acataggcaa 17400 tggtgatggc tgacagcatt gtgaatgtaa tcaataccat tgaattgtac actcaaaata 17460 attaaaatgg aaaattttct gctacataaa ttttaacaca atacaaaaag aaaaagtcag 17520 aaagaagtcc ttttggaggt gagtgggtgc tcctagtcac actcagagat agggagggct 17580 cactcttcct gtgtttgagg agatcctggg actggcagta catcctgtgt gtatatgtag 17640 aggcagatat gtcctaacag gacagccttt tctactctgt gtcttgaagt ttcacaggaa 17700 tcaggatgca tatgtggtcc caattctgtg tctgtgccag ctgttcctgt agctttcttc 17760 ttgaggggtc cagagcactt cagcatggag tttgccacaa cggcctctcc tctgtctgga 17820 ttgctgaatt cctgttgagc ttcctgggga atttgagagt agagggtggg acttggctat 17880 ggccgaatta ggctacttgg aaatcggagg ggaacaagta tgcaacctaa aacattctgt 17940 aggtcatttg tagttcactt gcagtcagat aaaatcatca ttagattaaa aaatgtgact 18000 tcatttatgt aacttttttg acaagatatt tattttaggt aaataaagct tacctctaca 18060 agagtcaaag ctctttttgc ttaagcttat tttttaaatg gaaatcaaac atgttgtata 18120 tacccttttt gaaatgtatc atacataatt taaacgcttc ctgatgattc acggtatcta 18180 tttttatttt ttatgtaata catttttttc ctatacacct ttctgtcatg ctgttattgc 18240 tgtcatttta actgccccta attcattctt tgatgtttct gtgctatggt ttaggatgct 18300 aaataaagag actcattaag aattatatat taaataagag caaatagtta tggagtcatt 18360 gcttggtttt acaaccgggc tttgggccag atatgtgggg tgtccagaaa ccaagttcaa 18420 ggttctggaa gagtgggcta aggacactgg aaatttggct tttgatatgt cactatatat 18480 cttttttttc aaaccgcaaa tagctctctg caaacagggc cagtgctcaa ctgtgcacca 18540 gttagaaaag tcaagtagta ggcttaaatt atttttatgt gtctggccag ggtataaata 18600 gtttaagagt ttagaattaa tacaaaatgg atttaagatt taaaattttt aactccctta 18660 ttctttgtta tatggtcact ggccgtgact attcttttca tcctctgggc ttcctttttc 18720 tctctctctc tttagagaca gtgtttcact gtgttgccca agctggagta cagtgatgca 18780 gtcgctgctc actgtagcct caacctcctg ggctcaagta attctcccac ctctgactcc 18840 tgagtatctg ggactatagg tgcacaccat cacatctggc taatttttta aaaaaatttt 18900 tgtagagtca gagtctcact atcttgccca ggctggtctt gaactcctgg gctcaaatga 18960 tcctcctacc ttggcttctc aagtgctgag attacaggcc tgagccacga tgtctggcct 19020 ctggcctccc tttctaatgg gtcttgtgat agaggctttt gtccccacag cctacttgcc 19080 attgttgttc tgactattcc tgggagaaag tgggtggagg gttcaggtca catctagtct 19140 tggctagtta gtacatagat tgttttaaac gtctttgaat ggccttcaaa actgcacaat 19200 ttgagcctgt ttttccagtc ttaatctgcc actcactctc ctccccaaga tattccaagg 19260 tcggggtagt tagggttgcc agatgcttat attaaaaaat tatttgttgt tgatctgaaa 19320 ttcaaatgtc accgggcaac cctaccacta gtgttgatga ccacaggagg gaggagagac 19380 tagagtttga ttcttgcctt ctgtatgcca ggcatggtca tatcagtcat attaatttca 19440 ttatctcatt taatcagaat cacatccaat cccagacctt ctgaatcagt gctaggtgtg 19500 gagcctggga aattgtgtgt atgtcttatg agcattccaa gtgattccta ttcaaaggga 19560 gttacagaaa caccatgacc tagaccagtg ccccccagac atcatgcaga tgcaggtcac 19620 ctgaggttag agctgaagtg caggtttcga tactgcagac ctggagtggg gcctcaggtt 19680 ctgcatgcca acaacctccc acgagacgct gaggctgctg gttgtgaatc ccacttggag 19740 tggtgctgtc ctgagcctcc cagccaagaa gcagcagcac ccccagcagt tgtgataatg 19800 aagattataa atgatactat gtttttaaat aatcagaggt ggggatataa gtggaagtat 19860 ttggcttatt caccaatata cagtaacatc tatgtatgaa gggtgaagtt ttgtgtgcca 19920 ttttttagga aacttttttt tttccaaaaa gtaaatgtct tacccgcagc tgagggatgc 19980 acattttttt cctccactaa gtggatattg gcttatttat gctggtcttt ctttcttata 20040 cacagtgggg ggacatctgt ccctaatcac atttacagct tcaaatccca gaatggactc 20100 cagatgtgca gggcacagtg tgacttggct ttatggaaat agtttctcag tgccagagtc 20160 atggtgatag agttataatg tgactccttc cattttcttc cctcctagct gcatgaaatt 20220 aaatggtttt attgagagta gcttagttta ctcagcagaa tctagttaga tcagaaatat 20280 ttaaggtaca actgtgtcaa agaactgatg gaaaatcgaa agagttaatc aaactgctca 20340 caaggacaaa agaaagtcct gctgcacgat cactttagcc ataaacatgc acactcaaat 20400 gaaggcagat tatgtaggtg acaggtggtc agcttctcag tccctttatc tgttgattta 20460 atatcttttt tttttttttt tgagacagac tcttgctctg tctgcaggct ggtgacaacc 20520 tgcaacctct gcctctcggg ttcaagtgat cctcctgcct cagcctccca agtagctgag 20580 actacaggtg tgtgccacca cgcgcagcta atttttgtat ttttagtaga gatggggttt 20640 caccatgttg gccaggatgg tctcaatctc tcgacctcat gatccgccca ccttggcctc 20700 ccaaaatgct gggattacag gcgcgagcca cggcacccgg cctctgttta tttttctaat 20760 ttgtagttat gagatgagca gaggttagat tggagatgtc tttgtggaat atcacctttc 20820 aaaatttagc agttctcaca cagaaagcac accagcagtg gcccaactat tagtctgttt 20880 attcatcaaa ttcttttttt tttttttttt tttttttttt tttgagacca ggtgtcactc 20940 tgttgcccag gctggagtgc agtggcacaa tcatggctca ctgcagtttc aacctcctgg 21000 gctcaagtga tcctcccacc tcagcctcct gagtagctgg gaccacagat gtgcactaac 21060 acgcttggct aattttttta tttttttgtg gagatggggg cctctctttg ttgtccatgt 21120 tggtcttgaa ctccgagcct caagtgatcc tcccaccttg gcctcacaaa gtgttggcat 21180 tacaggcttg aaacatggca ctcagcccat tcaccagatt cttacctagt cggtgcaagc 21240 cactgtaatc tgttgtatgg caaatacaaa gatcaaccag atacaaacta tctgcaaaag 21300 tcacttgtca tcagaatgac acaggaagca aaggactgag agattcattc tgtttgaagg 21360 aggagtggat ttttaaaaaa ggctttcagg agactgtggt atttgagcca ggcttcaagt 21420 tgtggataga atttggactt gcagagatgg agctgggtaa atggtgtttc tgaaagacag 21480 aacaaactga gcaaaggcac aaaggcatgg aaatcttgga tggagtattg tgaatagatt 21540 acagttccag tttgtcgaag tgtagcatgc ttacagggaa gcaattggaa acgtgggtgg 21600 gacctacctg ttgttggaga gcttgaaatg aattcatttt ggtgccaatg gggagtggtt 21660 gacgtgtgag cagcagaaga gtgacatggt gaaatgtcat ttgggagact gctgtgtgtg 21720 gaatggattg gacaggcaca aacttgagat gggaatcagc tgagacacat ctgaaatagt 21780 ctagccttca ggtgatgagg gccttgttgt gagtgatgga ggggagaagg tgtttatggg 21840 caaattcctg aaccttaaca accatgagct atgggtgcag ggaggagggc catgtcatat 21900 ggtactggga cctccagacc aggcgttatg ccttaatacg agagttctga ggttggcggg 21960 gaggtgggag agcccacttg taggaaggaa gatgtgtttg gttatcctga actgtaacag 22020 caagaattga gtgtgtcttc ttcacacagt gcccttagca gagcctgggt ggcaagtggt 22080 gccacccagt gaatctttgt tgaggttgag atgccagcca agctgctaag aggaagctgt 22140 ccaggaaggc ggttggaaat ggctcctggg gcctcacggg gaagctagcc ctagagatgg 22200 aacttgggca atcttggaat agtgtaacag agccctcagg aaagagaaag ctacagacgg 22260 agctttagtg atgccttagg gggtgagaga agtcctaaaa taaatggttt gaatgtaggg 22320 aaagtctcag ttgcctggag gccaagaaca aaaaggattt caggaaggag caagtgggca 22380 caatagatga gtgcttctga ggggacgggg ctgagaaaag gacacaggca gtggccatga 22440 gaaagctgtt ctggagggtg gcagggacag atgtcagatg aactgctcga tttggtttta 22500 atgtagattt ttaaagggaa ggaaacaagt cagtgtttaa acattggtta caaggtactt 22560 tgatatattt tttagataac gcttctaata acaaaagtat actatagact agagcagctg 22620 aaaaaatatt agtcatattc tgcaagggaa aatttccatc tttgtagcca tttgggtcat 22680 taagtgccag taatcacatc cagggtatgt atatatagga gatacgttgg caaaatagga 22740 gaggactggg tcagctgctg ctatctcatt ttatttattt ttccaaattg aaaactgtgt 22800 cagttacaag tacctctgga ttaaaaataa aatgaacttt gctgaaatgt taacatgtgg 22860 atagtcaaat agcaattata atgtggtaca gaattgccca gggcagaggt gtggtgggaa 22920 gacagctacc agctgatcac tccttccttt gatttgagct gactaaagtc agaggatcct 22980 ctaacactgc acactacaac tttgtccctg tgggtttctg ctggatcagt aaagaattct 23040 tctgcccccc agcccctccc aacactgggc acactaccgt tataaggtag ggatgtaggc 23100 agttatgtgg ctgatattaa tgtgatgttt atccctgtag gctggtactg cacatcacat 23160 ggtgtgtggg atcacagatc atattttttc tgccaaaagg cagtgaatta ttcccccaga 23220 gatgccatta tttcctcctt aaagagaaat aaattatata gctgctatat gggagtggca 23280 caccctgggt ggatcactct gaagcctgaa gcccaaggga gaacccagga aaggccaggg 23340 tactgccaaa ttctctccag cctgggaacg ttgcttcctc caaaccctct ggggcatctt 23400 gagctgctcc tattttgtag ggaccccgag ctctttcccc agctcagagg ctgaaaggag 23460 ttcctaggag tagcctctct ccctgagctt cctctcatct tagtggactg acttgagttt 23520 ggagggaact aagttgggag gctgagctca gtaaaatgtt gaccacccca cccttcctga 23580 tattgtacct tccttcggcc ttggcagctc agcatccttc tgatgtctct cggctctcct 23640 aatgttcctc ctgtagtctt ctctctgctt cctgaagagg gacttcccaa gagttggccc 23700 ttggtgcttg gttgtctgtg ttttgtgact caaattgcct ttcttagtca tccagcaaac 23760 acttatttca tatctacctc tgctacaggt ttcagactgg gtgatggagt tgcaaagcca 23820 tgatccctgc tcctgagagt ttacaggcca gagagggaca cagacatata acaaagtagg 23880 gcacctaatt tttacagatg ctataccaag gagattcaaa agaaggaatg gttagtgctc 23940 tcagagggca agggcaagac tggaaagatt tagtagaggc aaccttagac ttttctgatg 24000 atttccaggc ttgtggcttc aaatgttttg ttagatattc ccattaggtt gtgcaggaaa 24060 gaaagaaaga atggacagag gcttggaggc agagctggac ttgaatccta gatctgtcag 24120 ttaggagttt accactctgg aacaaggaaa caggtaatat tctttatctt gcagattgaa 24180 aaatactgtg atcatcactt ttccagagct tctcatcctc agttcctaat caaggggtgt 24240 tagcatgcag acaagaagca gggtaaggag actcatcagt gacctgttag tagtcatgga 24300 ctacaaggta aagaaagcat ggaccacaga gtctgttacc ttagtgaggt gctgtcagga 24360 ttggtgacaa tgtacaaaaa gcatctagta ctagaacagt actaatcaat acatctccca 24420 ctcattgtca acctgctacc agaaagccaa gtgactcacc ttccaaaaat gccctgttcc 24480 ctcctttttc ttccacagct gtcagtgcta atggtgtcct ccaggatcac tcagctttag 24540 tatctgcctt ttctgtcttt tgcttccttc gtattccctt gccttctctt tctgttaatg 24600 tataaaacca gtgatggcct gatttgaatg taagggaaga tgatgaccac tggggtcact 24660 gagagccaca gatttaagag ctcttaagca ttcattgttt gaaacaggat ttccctagag 24720 gaaatttgtg catttattca atgaggatgg aatcactagg tggtattcag catctgtctc 24780 agagctcatc ctgagtgcta cgctgcatca cactccggac cacacagagc tgcatgcata 24840 gcagaatttg tactctgcag actctacgga cacctggaac ccttaagagt agatgtgaat 24900 gtttcacttc cgccatgata gcatgagttc tgtggacctg atccacagaa aaactaaaaa 24960 ttactaaaga acataaaact aaccacttaa agtatctgga aatggcctgt aaaccaaaaa 25020 gtatctaaga caggtcttaa tcaattttga ggtttatttg ccaaggttaa ggatgcgtcc 25080 agagaaaagg aacacaaaac cacaggaaca atctgtgata catgctattt tccaaagagc 25140 gttttgggac ttctatattt aaagggaaaa agagcaggta gtagcacaaa gaggaaagag 25200 aaaaataaaa gaggagggta gataaaagaa gagaggggtt gcattccttt gagtctttga 25260 tcagcattca ctgaattcac attttacctt tgaaaagaaa gcatagagaa atagtcatta 25320 tgcattcttc tcacactcag taaattagga ctctacctaa gataaagtaa acatagagtg 25380 gctatttgtg gagccatctg gccttctatc tagccttctg tcttctaagt tacttgttta 25440 gaaacaaaaa gaaaggcagt tgcttacatg actcagtttc cagcttaact tttccctggc 25500 atagtgaatt tgggatgccg agattttatt ttcctttcac caggcatatg ggcattcagc 25560 aaattaagaa atacttattc aggaaaatct actaaaactc agttacaaag gtgagagctg 25620 tagtatttga agcaagacct ccacttttct tcccccactc caaactcaga gagatggaaa 25680 cttcactcta gactggtgca accaagaatg cagagctcct tctaccccag ctcctaattg 25740 gagacctgtt gtcttggaaa ggacagtatg tcagcctttc tcatcttgcc ctcagcaacc 25800 tgtttttgag actaagttcc aggcaagtgc agcccagagg tgtggggttc tttctttcat 25860 ccagcctcca cccataggac agaggaatct accttgaatg tggcgtgctg agaatcctgg 25920 gacctacatt atccttgccc ttgcttgaaa gctgtggttt ctcacaggaa gaagcaagct 25980 gagaagacct atggttactg ccccaccacc actgagaact cagctataag agtaggggtg 26040 tcacttagag agaagcatac cattgtctcc atctgcagtt ctagagccct aactcagaga 26100 tttgcctggg ggagaagtag gttgtaaaac agagacctct cagtatctta ccaaaggagc 26160 tgactttgtt tgcaacaaag tgtggagaaa tttatgccca aggtttctct caaaaaaaaa 26220 aaaaaaaaaa aaaagtagag gttgtagtga aaggcaatgg ggaagagatt ggtagattca 26280 ttggagatat agattaagtt gtaggccagc tagtttgctg ggaactaggg aaagagacag 26340 ctgagaggag cattcctgga gtcagagtga atcttaagca ctggccttgg aaactgtctt 26400 ttcaaaggag ccaaatttga ttgaatcaat ctaggaaaca acatataccc atagcaatca 26460 accttcagtt aattcaacag ctaggaatgg tcagggaaag agatagtgaa acagaatctt 26520 gccaaagccc tattagggta gctgtggaca tacccaaggc tacatcccct gagtggtaac 26580 atcagaggct tcattctatg gggggaagga aacagacttc agtaaaataa tccaactaat 26640 cactaaagaa ataaacaaat cacaatagca atccctggag agagggagga tctatcatcc 26700 tgattgctac aatatgttat gtagaatgtc cagtttccaa cagaaaaact atgagatatg 26760 ccaaaaaacg gaaagatatg acctatacac tagggttagg ggttaggggg cattaggcaa 26820 tagatactgg ctgtgagagc aaacaggtgt tgaatttaaa agaaaaagac atcaaagtat 26880 gcataataaa tacattcaaa gagctaaagg aaacccatga ttaaatacat aaaagtaggt 26940 attatgacaa tgtcatatca cacagagaat ataaatgaga tataaatttt aaaaaagaac 27000 caaatgaaca ttttggaatt gtaaagtata ataactgaaa tgaaaaatta accaaagagg 27060 ttcaagagta gatttgaact gacaaaagaa agaattaatg aactagaaga aagattgaca 27120 gaggttatac aagctgaaga gagaaaaaaa tgaacagagc cttagagaat tacaagacac 27180 cattgagtgc accaacctat atgtaatggg aatactggaa ggagaggaaa aagagaaagg 27240 agcagaaaaa atattagaag aaataatagc ttttcattat ttccaggctt tccaaatgta 27300 ttggaaaaat attaatgtac atatctagaa agctcaaaaa actccaagga agattagcaa 27360 acacaaagag gtcagagaca ttatagtaaa aatgctgaga atccaaagac aaggagagaa 27420 tcttgaaagc agcaagagaa aaatgactta ctacttataa gggaacccaa agactaacct 27480 gacttctcct cagaaatagt agaggccaga aaatagtgat gtccagattc aaattacata 27540 aagaaataaa tgttaactaa aaatcttata ttcagcaaag ctatctttca taataaaagc 27600 aaaataatga tatccccaga tgaacaaaaa cagaaataat ttgtttctaa cagatctgcc 27660 ttacaagaaa tgctaaagtt atttagtctg aaagcaagta acttcacaca ataatctgaa 27720 tcttcaagaa aaaaacaatg agcatatata tgtgtaaaag acagtattaa tggatatatc 27780 acctcccttt ttaaaaactg atttaaaaag caattgcatt aaactataca catatataca 27840 cacatacata tatatattat cattgagact tcacttatag aaatgtagca tatttgctaa 27900 taatagcaca aaggaggtag gtggaggcaa tattatattg ggctaagaaa atgactctag 27960 tggtaacgaa ttcacataaa caaacgaagg gatccacaaa tgataaataa agttaacata 28020 acaaaagata taaatatgta tatatatatt tgttctcctt tcttcactta gcttccttaa 28080 aagacataat gttatataat gtaataatta ataagaatgt actattgggc ttataacatt 28140 ttaaatgtaa tagtatagtt acatcacaaa aggggagaaa agggaataga gctatataag 28200 agtaacattt ctatatctca ctggaataaa attggtgtaa atctgaagct gattctgata 28260 agatgtatct gagaaggcct agagcaacca ctaagaaaat gattttttaa agtagtaaaa 28320 aaaaaatcct taaagaaatt taaatgctat agtagaaaat attctcttaa tgcacaagaa 28380 agcaataaag gtggaataga ggagaagaca acatgggaca tataggaaac gaaaagtaga 28440 atggtagaca taaatttact gtatcaataa tgacattaaa tacttttaat taaacagtcc 28500 aatcaaaagg cagagagtta aacaatcttt agttaaaaac aaaaagcaag attgaactat 28560 atactgtcta cagagccaca ctttatattc aaagatacaa atagactgaa agtaaaagga 28620 aaggaaaaga tataccatgt aaacagcacc cacaagaaag ctggagaggc tatgttaata 28680 tcagacaaaa tagacgttaa aaaaaaaaag tcaccaaagg taaagaggga cattttatag 28740 tgataaaaaa gacaatttat taggatgata taacaattat agacatatat ccagtaaaca 28800 acagagtgcc aaaatatgtg aagtaaaaac tgacaaaaat gaaggaagaa acagataact 28860 caacaatggt agttgaagac ttcaataccc aattttcaag agggatataa caactaggca 28920 gaaaacaata agaaaataga aggcatgaac aacactaaaa gccaacaata cctagtagac 28980 attctcagaa cactatgaat aattaatacc aattctttac aaattcttca aagaatagaa 29040 gatgaaggaa cactttctaa ctcattattt gaggccagta ttaccctaat accaaaaatg 29100 gacaaaaaca tcacaaggaa ggaaaactac aaaccaatat tttttattat gcatggaaaa 29160 aatttctaac aaaatactag caagctgaat ccagaaacgt atgaaaagga ttatacacca 29220 gggtgggatt tatctcagga atgccctgtt ggtttaacat cccaagatca attaatgtaa 29280 tgtaccgtat caatagaata aaaaacagaa ccatgatcat ctcaatacac agaaaaatca 29340 tttgataaat tcaacacctt ttcatggtaa aaacattcaa aaaactagta ataggaaaca 29400 tcctcagtct gataagacat ctacaagaaa ctcacaggta acatcatact tgattggaaa 29460 agagtggata ctttttctct aagatcagga acaagacaaa gatgcccact cttgctattt 29520 ctagtcaaca ttgtactgga gtgtctagct ggggcaatta agtgagaaaa ataaataaaa 29580 gacatcccga ttggcaagaa gaaacaaaag tatctctatt tgcaagtaac ataatcgtgt 29640 atatggaaaa tcccagggac tccactaaaa aaccattaaa actaataaac aagtccagca 29700 agtacattag attcctgggg ctgccataac aaagtaccaa aaactgggta acttaagaca 29760 acagaaattt attttcttac agttacaggg gctagaagtc caaactcaag gtgttgggag 29820 tccctttgag acactgagta gaatccttcc tttccttttc tagtgtctgg tatttgttgg 29880 cttttcctgg tatttcttgg tttgtagatg catcactcca gggtctgcct ccatcatcat 29940 atgactgtct tcatccttcg tgtctctctt ctcttcttgt aattgcacca atcatattag 30000 attaagggcc taccctactc tagtatgacc tcaccttaac taattacatc tgtaatgacc 30060 ctacttctaa ggtcacattc tgaggtagtg ggagttagta ctccaacata tctttttgat 30120 gggggataca atccaaacat aacagcaaag ctacaagata caaaattaat tactaaaatt 30180 aattatgtat ctatatacta gcgttgaaca atctgaaaat gatattaagg aaataattcc 30240 atttataata gtattaaaaa ataaaatact taggaataat tttaataaat aaatacttca 30300 aaaacttaaa aaaaattgtt gaaagaaatt ttaagactga aataaatgga aggacattca 30360 tgatcctaga ttggaagaca aggttaaggt ggaaatcctt cgcaaatgaa tctacagatt 30420 taatgcaatc actatcaaaa tcccagctag attctttaga gaaatttata ggttattata 30480 gaatttcaaa actaatatgg gatttcaaaa catacggaat ttaaagggac ccaaaatagc 30540 caaaacaatc tcaaaaaaga aggacaaact aggaggcact taacacttct caattccaaa 30600 acttactacc aagcaacagt aataaagact gtgtggtact ggcacaaagt tagacataca 30660 gctctatgaa acggaattga gaatccagaa ataaacctct atagttaatt gattttcaac 30720 aggaatgcca agaccatcta atgtaggaaa aatattattc gcaaaggatt cttaaatatg 30780 acacccaaat gcaagtgagt aaagtataaa taaattgggc tttaataaaa ctgaaacctt 30840 gtgtgcttca aagcacactg tctagaaagt gacaaaacag cccacagaat gggagaaaat 30900 attttcaaat tatatatctg ataagagaat tgtatccaga atatgtaaag agctcttaca 30960 atttaataag acaacccact taaaatggca aaggacttga atagacattt ctccaagaaa 31020 tattttcaaa tgaccaataa gcacatgaga agatgcttgg catcattaat tatcaagaaa 31080 tgcaaattga aaccagagaa gatacccctt caaggcccct aggatggctt gaatcagaaa 31140 gtcagataat aagaagtgtt ggcaaggatt tggagaaatt gaaaccctca cacactgctg 31200 gtgggaatgt aaaatggtgt gaccactttg gaaaaaactc tagcagtttc tgacaggtaa 31260 tcacagagtt attatatgac ccagcaattc cattttttag gtacataccc aagaataatg 31320 aaaacaaaag tcatggaaaa acaaaaacat ttctaataac actgttcata gtagctaaaa 31380 ggtgaaagca acccaaatga ccataaatta atgaatagac aaattttgaa tatgtacaca 31440 gtgggatatt acttggccat aaaaaggaat gaagtacaga agcatgctac aacttggatg 31500 agccttgaaa atgttatgct aagggaagga aaccagtcac aaaagacccc atttcattta 31560 tatgaaatgt ggtaaagcta ttgagacaga aagtaaagct attgagacag aaagtagatt 31620 atctgttgct tagggatgcg gtggatgaga ggatgggatg gcggtggggt ggcagctaca 31680 aggtacaggg tttctgtttt cttctctctc tgtcacgcag gctggagtgc agtgtagctg 31740 agacaacagg cacatgacac catgtccggc taattttctt ttttattttt gtagagatgg 31800 ggtttcatca tgtttcccag gctggtcttg aattcctggg ctctagcgac ccatctgcct 31860 cagcctccca aaatggtagg attacaggta caagccacca cactcagcct aggtacagga 31920 cttattttga gctgatgaca gttttccaaa atggattgtt ttagcagttg catatatctg 31980 taaatatact aaaaactcct gaattttacc ctttaaatgg atgaattagt acatgaatta 32040 tatctcaata aagctgtttt ttaaaaagag agtagacatg taaggttgct tggaaaattg 32100 actgcttgtg ttgtaaagtt aggccaagta tatctgtata cattccttgt gaacagtgcc 32160 attttagtga ttatataaaa tccaggacag aaacaataaa gtggtgggac cttatcacct 32220 agttctccag tttgaaatta aacctgttcc tcagttctat taactgtagc ctacattttc 32280 atgtcactga tcattatact tgggctattt atagtcttta gactgatgct cttggataag 32340 tttggcttag aagaaaaaaa aaagctattt tcagagtaat aagctctgtg agccagtagg 32400 ttaataagat tatagcaaca ccaaaggtat atttttaaaa atctcaatga catggtgatg 32460 ggaggggatc ttgtcagata atgatattct tgaggttgaa gcaatgttaa ctctttagcc 32520 ctagtacact cttttttccc tgaaatgttc aatgaatgct tccaaagaaa tggcagaatg 32580 ttttgttatt gttctgatct ttatgttgcg atgttacaaa tattgtaact tattttaggg 32640 actttgtaat tgtcacggca cccacagtac actgaatgct gtatggagca tgagcatata 32700 gaagacaggt acttattcat ctgggctttg ggctgagaat cgggagtcca ggtttctcaa 32760 gagttgggtt accgattagc ttagtccaga ctcatttgtt gagtaggagg attgtaccag 32820 atgcttcctg acagtcttat cacctccaac ttctgtgatg ctgaactcct gtgaaagatg 32880 gattatctct gtattttagg cagaaggaag ttagtacaag tactacatac atgtcacaca 32940 aaatcctttc agataattgg gtagcagtga catttctgga gattaactgg cttacacgaa 33000 ttgggccctg gaatgtttca tgggacatga aaactgactt gcttgatcct gtctttttac 33060 tacagatatg ctcctccacc tcttttacca gtcggtagtc ctgagagcag ggttttccaa 33120 actggatttt gtaaggtaat aaagtttgcc tgatgtcttt agtgcaggac ttctcagtta 33180 ttaacatgct aacttgagac aaccaataca acatttccaa aatgaagaga ctctgatgaa 33240 gccctttata tcatggagca catattaata cttcactgaa ccagaattca gaggaatcca 33300 gtttagaggc ctgtctgcct tggacacctc caaataggta taaaaattca ctgccaccag 33360 aaaacctggg cctgaccctg cccagtcccg ccttggtgct ccattagccc cacagttcca 33420 gcctcactga caggtcccct tctcctcttt gaggtcattt tctttttctc acacagattg 33480 aacattttaa gaattttaat aatgaaaacc gaattagacc tgttctgtgg ttgcactgtg 33540 ttgattcatc cttgcgttac cagtgcctgg cacagttcct gacattataa agcctggcac 33600 acagttatta ctcaagaaat gtttgtggaa taataattga atgaacaaat gaatgtgtac 33660 atgggggaca aaagccagct aatctctctt ctaatcaata tttacgcatt aagcttgcat 33720 agtataaatc agctctgctt gttaaggatt tttcccatga accagagctt caggtttgag 33780 atctgaaggg tgctgtcaca ccagctagtt tataaccacc tgtactggtg ccatgtcttt 33840 tattggtggc attggcctga ctctgcccca gcaccacctt ggtgctccat tagccctgca 33900 gttccagcct cactgacaga cccccttctc ctctttgagg tctttttctt tttctcacac 33960 agattaaaca ttttaagaat tttcgtaatg aaaaccaaat aagacctatg atgccccatc 34020 attataaatg ttctactcca agaccaacat cttctttgca cccttgaacc tgatactttc 34080 ccccatgtcc tcagaaggag aagcttttat gtattctttt tgagtttcca taaactcagg 34140 ctcagggcaa atggaaggaa cctctgcaaa tgcaaagata aatgtctcct gagctcttcg 34200 gttagggaca gtagggcaga cactgatgtt ggagcttcct ggctggctat atgccttcta 34260 caatcctcag agtccaatag gtatgctgta aatactttca ccctactgtc ttctgaaatt 34320 tgggaaaata ggtattcacc catctatgct cttctttcaa caaagatggg gattatttat 34380 gccttaagtc agctgtcaag tttaaacaaa gaacttcaac tacctccttt cttaaacttc 34440 ctaaagcact caagtcatat gaaaacaatt ctttaagatg ccatatttct tttaaataat 34500 cttatttaga agtattaaag gttattttga tacccctggc tcctttgata cctttcagaa 34560 gcttataaat tgttaaagaa ttacctagtt ccttttccag aaagtctcaa gatcattttc 34620 catgcaacag ccacatacgt tagggaccat tatttgtgtc agaaagaaat ttacgagctc 34680 cacaatagga tgtcctatgt agtagatcta tgtggtggaa aacaattgcc aaaggaaaag 34740 tcagctagat ttgagtgatg tggcacattt aacttatgtt gtctccattt tatatgccca 34800 cagtttgagt tctgccagct gaagttaaat catgtgacta agctggtttt tggtcttttg 34860 gttttcttaa taccaaggct ttttcaactt aacactgaat tccatataat gctttcatta 34920 agaatgccat gcattagact ctgcttgtca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 34980 nnnnnnnnnn nnnnnnnnnn tgggacactg gttccaggac ctctacagat atcaaaatcc 35040 acagatgctc aagtccctta tataaaatag tgcagcattt gcaaataacc cacccacatc 35100 ctcctgtata ctttaaatca tctctagatt acctataata tctaataaaa tgtatatgct 35160 atgtaaatag ttgttatact atactgtttg gggaatcatg acaagaaaaa aaagtctgta 35220 catattcagt acaaatgcaa tcatcctttt ttaaaaaaat atttttgatc tgcagttggt 35280 tgagtccaca gaggtggaac ccacaggtat gcaagtcttg actgtgtgtg tgtgtctgtg 35340 tgtttatgtg tgtgtgattt ttttaacagt ttaagactca atagaagttg tagcaatagt 35400 acaacattcc tgtttaccct tcacccagct tcccccaatg ataatatctt acataacctt 35460 agtacattgt caaaaccagg aaattgacat tggtacaata ctattaactc aaatacagac 35520 tttatttgga tttcaccatt tttttttaca tccactgttg tttctgtttg tgtgtgtgga 35580 ggaatggtat agttctagga agttttatca catatgtaga tttatataac ctcaccagta 35640 caaccagggt ccagattgtt ccatcaccac aaagaaactc cttcatgtta ctccttcaca 35700 gccataccct gtcctcaacc ctaacccctg gcaacaatta atctgttctc tatctctata 35760 attttttcat tttgagaatg ttatagacat ggaatcatgt tgtctgaaac cttttgagat 35820 tggctttttt tttctttttt acttagcata gtgcccttga cacccatcgg gtggtatgtg 35880 tattaatctt ttgttcctat ttattggtga ctagtatgag caatatacat tgagcttgga 35940 tttgttggtg tatttgttgt taggttaaga ccaaagtcag aaagatcaaa tcagtagctg 36000 agtagattca ggaactggaa tcaaacaagg caaggttggg gctgggagag aggcagcagt 36060 acagagcgga gtggaaagca ggaactgaat tgagattgag agtgagagag gtgaggccat 36120 gctccaaccc agaccaggag agtgagaact gcagcagagg catgcccaga atcaactcca 36180 gtcaagaatg aggggttccc agaaggaaag ttgagcaggg gaagttggag aataatagct 36240 gagtgttccc tccacctgtc cctggcttac ccaggcctta ggggacactg ttctcctgga 36300 atttcctgat attgtttctg ctctgtagta tcttatgtta atattatgcc cagaatagaa 36360 ataaagatac cccgtggtca aaagttttat gcattttttc aaagtaaagc aaattcctat 36420 gcattgacct aacacataat gacacagtag cttccacaac cctgcagtgt ggaatgctgt 36480 gatctggctc aaattccaaa gctttttcat ggtggggaag aaggagagag aagaagaaac 36540 tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 36600 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 36660 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 36720 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 36780 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 36840 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 36900 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 36960 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 37020 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna cccaggaacc cgaatccaaa tctttatcag 37080 ctgagttata ctgaagcttt ttgtttttaa tcccaaccta gaagaggcac tttttaccct 37140 tttttagtcc taaaatgata caggaaagtc aggagctcag tatgtggggc agagaaacag 37200 cttctaatct agagacaaaa ttcatggaat ccacattcat ggatatagct tctaattgct 37260 ttcttcttcc ttgcaatgaa atccagtcgt ttgtaaatcc tggttaggac atcttggtag 37320 aatggtcctt gagtttcctg tataaatgca caagtctcca atcaccctca ggcttctcga 37380 ccctaaacac aagctactga cctcttctag atagagtcaa ggtccatggc ccagaggtac 37440 ctactcctgc ccaattgtat ctcctcccca gtcctgcatg gggagggctg aactgttttt 37500 cactggatgg ttccttctgc cagtgcagcc ttcactggcc ttccccgacc tctgggactg 37560 aaccctgtgt ccctgtctaa tgctcgctac tttagctgac tcaactccat gcttgcccaa 37620 ctgcagtagc tgcccccacc cctgcccacc aggatgactc accctggacc cctccttacc 37680 ttggatccag tcagataata atatgtggaa ctcctttacc acctttaatc aggttggcca 37740 aaaacagtag cagaaaatgt gctcataaaa ttctgaatct ccttttggag atgatgtcgt 37800 gaaggaggta tggtgatctt tgaccaaatg ccccaagatg gtcggctccg ggccgcgctg 37860 catttcttat gccgcattgc tctctctccg ttcattttgc tttcacgata gcctggagtg 37920 aatatatctt cccttccttg tcagccctta cttctctctt aaattatgtt ctactgtgtt 37980 ctcacaagat tttctcgtaa aatataaaac caccttcctt ttgggagaat atgtagactg 38040 tgggggtttc agtagttctg acatcatgtt ccagggnnnn nnnnnnnnnn nnnnnnnnnn 38100 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 38160 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 38220 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 38280 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 38340 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 38400 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 38460 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 38520 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 38580 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 38640 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 38700 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 38760 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 38820 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 38880 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 38940 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 39000 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 39060 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 39120 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 39180 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 39240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 39300 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 39360 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 39420 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 39480 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 39540 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 39600 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 39660 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 39720 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 39780 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 39840 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 39900 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 39960 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 40020 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 40080 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 40140 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 40200 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 40260 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 40320 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt actgatgtga cgagatgggc 40380 tcagatctgt atgactaggg cactgtgctt agcatctaga catgatgtaa tattcacagg 40440 aaccctctta gttgtagggg gcagggtggt gaaaaactgg gaattaaggg tattacaaag 40500 gcataagtag gtatatttta tctcagactt gtcagttagg attttgtttc taaagccgac 40560 agaactactg gatcaaagaa gatagcataa aactgttatg tttctcagta gcactacggg 40620 aatccatcaa ctgcatcaaa ccttccccaa ttaacaaaag cttgggtaaa tcctgcatca 40680 actgaagact ggcattcata atgcccttaa ctggttccat cacattaaac ccattaagga 40740 agctcacaga cactgttgat gctgcttgga aaccagtcct agcaacaata gcacgtggaa 40800 tttgaggggc ttgagaaagg cagccctcga ggaattagct tgctaggtag ccagatcatt 40860 tggccagctg tggaaaccat gttagtggaa tagtggtgtc aatttgtgga gaaagattgt 40920 gtctggtagt aaaaaagtgg tttccccaaa gaaggtcaga gatgtcttct agagggctgg 40980 gtggcagcta ttagaagtca aactatacaa gaaagatatt ggtaaaaaat aataaataaa 41040 taaataaata annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 41100 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 41160 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 41220 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 41280 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 41340 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 41400 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 41460 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 41520 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 41580 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 41640 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 41700 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 41760 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 41820 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 41880 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 41940 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 42000 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 42060 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnta gctgtgttaa 42120 tcaaagcgct tattttgtac aataataatg accttgaatt tcttaaaaaa aattacaata 42180 agctacaagt atcaaagaag cgaagttatc tggagtagtc tatataggag ctctttggac 42240 tttcttttat tatgctaaaa tagtggtgct tttaggattt acattattgt actctccaat 42300 acaaagtatg gggcatgtta aagtatacag tacaccattt tcatacatgt acaacattgg 42360 tggatgaaga atgtctctta gcagtaatac tggatgtagc ctctggtttt accagctgca 42420 tactctagga ctattatata agtaaaaatc tctcttgtga tactggaaag tgattagaat 42480 gtgcaaactg atatagtagc tttcatccgc ctcttaaagg gtaccaccac aggaaagtcc 42540 atttaagatg ttggtaggtt taacaaagtt ggaatgctgg cactgttgaa ttgggcaaca 42600 gttcttcaga cctggctcag agctacaatg catttagtat attaaagcag ctgacatgat 42660 gactttttgc gagccttccc aggcactgga gtttttctgt taatttgccg cactaggtca 42720 taaaagatct cattaacatt tatttttgat tttgcagaag attctaagaa tgcacagttg 42780 ttccattgtc ttgctagatt ttgaccttgt tccttcccta caactctttc atcttccaag 42840 tcacacttat taccaacaag aatcattgga acatcatcag tgtctttaac tcgaagaatc 42900 tgttctctca ggtcttgtaa atcgttaaat gtggactgtg ctgtgatgga ataaactaat 42960 gcaaaccctt gtccattttt catgtataaa tccctcattg ctgtaaattg ctccgttcct 43020 gcagtatcca agatttcaag catacactgt tgtgcatcta cttcaacttg ctctctataa 43080 gaatcttcta tcgtaggatc gtatttttct acaaaaattc cttgaacaaa ttgtacagtc 43140 aaagcagact ttccaacgcc tcgtgagcca agaacgacta gcttatactc acgcatgatg 43200 caagcttgtc aaaacctagt actctgcgaa cctctcacgc tgtcaccggg tctctgcagc 43260 cagcgtcgcc ccgcgctccc cgcgggtcgc tactctaggc gccacggcgg tcctgccgct 43320 gccgcgccgc tccggctggt ttacacgcct gaatctgggc gaggttttct caatcctatt 43380 ttatgaaatc catctgcctc tggtgtttat gttggggtgg gagttaataa tgaaagagca 43440 aaataatctc ataatgtact tgttcccatc aggcctgcaa tgtgtgtctc tgatggaaca 43500 agaaagagag agatcctgaa tcttctttgc catggcactg ccactcccac ctcgggccct 43560 aagcacagac gatgctatag accaggaatc accaagcttg tctgtgaagg cccagatagt 43620 gaatacgtca aattttttag gccacctgtg gtctctatca cattttcttc tttactgtag 43680 ccattttaaa aaatgtaaaa aagaatgctt agcttgtacg ctgcacgaaa gcatagttcg 43740 tggacccctg ctacaaacct gcacattctt tgttactagc taagaatatg tctccaaatg 43800 tggacatggt ctcctatcct gataagcaag ggtcctcctg ttcatctagc ccaacgctga 43860 tgctggaggc aggctttggc tcctgcttca tgccctcttg gccagcacaa gtgctgatac 43920 cctgtttact gctgttgctg tgaatgttaa agtctcagag ccaagaatct catgtgttct 43980 gctggcaccc gtgagcctgg cgggctaact gactaaattt caggtaaggt gaaatctcag 44040 acccctcaca gttcttgaga gaggcagaag gggaagggaa ggagaggggg catggtgaga 44100 gataccagaa aaaacactac acggaaaaga gatgacccaa atgaagaggt cagttcccca 44160 acccctacgc tggcctggtg tttaagtcca gtttccatgt tggccttcac agaatcagag 44220 ctcttctttg ctagataaag tgggggggga gtctttggct ccccctggag gctagagttt 44280 gccaacgaca ggtaggacct gaaacacaat atgaattgct ctttactgta aaacaaaagg 44340 aaagctgacc catgacgaga ttttactcaa atggcccagg ggttaggtat aattcaatta 44400 aaaagggaat ttgtccttta aagtaggggg atatatatat atggctttaa ctaaaaagaa 44460 aaatggacac agaatctaac ggtgaatgtt taggtattag tgacagtcat cattgttaaa 44520 gtgcagagag aaaattagat ctaacacaga agagtaacat ggtgctaaaa tttcccgcat 44580 atcccagaca acttcctgtt tgcagctcga tgtgctgatg agatactttt aacacgaatc 44640 ctgagggcag tggaccttag aaaaattatc atctacaatt gtgtgttaaa ttgctaagta 44700 attaagatgt aagatctaga aaatcacata caatttattt ccaacagctg aaattagaac 44760 tttctaaata aaagctaacc atacaaccca agggtttaac aagtcagagt ctgtttggga 44820 aagccagcgt gtgtgtcccc gagaggtgga ttgcaatcag taataaaggc atgagtccag 44880 ccctgtcaga tctgtctgac agcccctact gcactctgtc agaatctccg catggggatg 44940 ggtcaggttg cagatggtga cagcatcatc cgaaagtcta tgagaaagtg aaggtaacac 45000 aaagccctgg gatccctacc ttccacactg ctctgcttat ctgcacacac agctgtgagg 45060 ctccagccag gcccccagga gcaatttgtt atttgtgaca accttgtgtg catggtgttc 45120 tccctcaccc cccaccccaa cacacacaca gggagccaaa gtttactacg tttagtctaa 45180 gttgggtttt gagatttaaa tgttcaagac ttaagtgatt accgatgggg aaaataggaa 45240 gtggcctgga ggttctcagt tcgggggaga atggttgtgc aaatagaaaa ccatggtagt 45300 gaatctgcta cccagttttc taagaagagc tgcggttttg tttcatcctg tctcagagct 45360 ttattaatca gccatattgt aggtgacatg ctccagaaca cacagaattt atgtccttgt 45420 gctctttcct ctaagagcta attaatgtat ttttattggt gagaaagttc actccacata 45480 tttcttctcc ttgcatcatg atcaattctc caatcttaaa accaaatcac ccaccaccac 45540 cacccataaa caccctctgc ttcaatcctg gtccccattt acttaattcc catctttccc 45600 ctacttttaa aatctattca ttaaaacaaa acaaaagcca aaaacatctt tcctgggatt 45660 gcctggagaa gacttgacag gaccaagagg gaaaactttc taacccccaa tcactgttta 45720 gtttatggct ggaattatgg gggaaaagta gaatgaggtt gcttttgatg ttcttttgaa 45780 atgatttgca gcattgaaag aaatattagc aattaagcca ttttgatttt ctttaaaact 45840 gaaagtagag gccggcaagg tggctcacgc ctgtaatccc agcactttgg gagaacgagg 45900 tggatggatc acaaggtcag gagatcgaga ccatcctggc taacatgatg aaaccccgtc 45960 tctactaaaa atacaaaaaa ttagccgagc acggtggcgg gcgtctgtag tcccagctac 46020 tcgggaggct gaggaaggag aatggcgtga acccgggagg cagagcttgc agtgagccga 46080 gatcacgcca ctgcactcca gcctgagcga cagagcgaga ctctgtctca aaaaaaaaac 46140 aacaaaaaac ggaaagtaca gaagtgtata tatttaataa tcaattatat taatcaatat 46200 attatatatt aatatataca aatatattaa tctatatata tatgattctt aatgctgtat 46260 ttcttatagt ctgggtatcc cagtgaatga aatattttca gcagtgtttt gggaagtaca 46320 gagatcttcc cttatatgtt ccaggacccg ctagtgatac ctagaatcgc agatagtacc 46380 gtcccctata tatactgttt tttcaatata tacataccca tggtaaagtt caacttacaa 46440 attcagcata gtaggatatt aataacaata actgataata aaataataaa ctagagtaat 46500 tataacaata tactataata aaagttacct aaatatggta tttctgtctc tcaaaataac 46560 ttaatatttt cagaccatgg ttggctgcga ataactgaaa ccactgaagg caaaaccgca 46620 gataaggggg aactactgta tgctattcca gatatgtgtt attatgtccc aaactatcca 46680 aaatcttagt ggctttaaac aatagccatt ttattgtatc tcacactcct gtgtgaattt 46740 ggaacttgga caaggcttgg ctaggtgatt cttctgttct tggtggttta aacagaggtc 46800 actctggtat tttgctggag ggtctaagat gactgacatc tgggcaggaa tggctgaaag 46860 attgttttct tgttctgttc ctctaaaata ccagttcctc ttagatattt atgctttgtt 46920 tgttcactgt gctgatatag aggagatttc tctgaaaaga aaaccatact ttcattgttt 46980 ataattatgc ttaaacttaa gaagataaat acatttttca taagtctgtt tctcatgcat 47040 tttgggccca gtggggacag taggtgagag cacctatctg tgacctcttc agcatatcag 47100 tctcagggta gtcagatgta cagatggggc ctcagggctc caaaagcaag tgctatggta 47160 gacaaggtgg aaactgcatg gccttttatt acctaatctt gaaagtcaca tagcatcact 47220 tctgtcatac actattcatt caagcagtta cccagaatca aggagaggca acatagactt 47280 caccttttca tgggaagagc attgcagaat ttgcagctgt gttgtacagt caccaaacat 47340 gcacacagga gacatctcca ttctattaca tgttagagta aatttctttt ctttttcagg 47400 tgtagtattt ccaaaatatt gttcccacca ttgaatttta tagcagtggc tcccagtctt 47460 ttggattttt aattccaatt ttgaaaaaaa agaatgagca agattgacag agggaagccc 47520 acctttattt tactaaggaa gaacatttaa aaataatgat tgcaataaca tgcacactac 47580 tctttatact cactatcatt ttataaaaca aaaggcatgt tcacatcaaa ataataggaa 47640 gggcatgttc ttaaaggacc ttttttaaat tgaataaatt tatctttatg aaaaagacat 47700 atcattttcc ttgtttttct tgtttaactg caggctggtg gaaactgtct catggagcct 47760 gcctttttgg cagattggat ttgggaatta ttcttctaga caaaaggtct caagtatgcc 47820 acttatactt tcttgggaca ctcaagattt tatggtcata atttcatttt tgagttagtt 47880 ctgtcaattg catagcttta tgtgaggagg actgcagttt gagttcaact ttctaaatta 47940 aatcaacgag gttttattga ttttcaaaat gtgcctacaa tagtgtagcc tcatggagtt 48000 tcagaaaagg atgacataat acatagttcc tgccaggagt caaattgaaa attaattata 48060 gagataggac tgatgcttat gaaatgctga taaataatgc aagttagaat gtgggtgaga 48120 atgtatagga gttacccacg caaactatag tagtctgggt gtcttcacta tgaatgtgga 48180 gttgaagttc aaggtacacg atttggatga aaggcaaaag gagcatgttg catatatgat 48240 taattgaatg aggattcgtg gataagaatg aatgaacgaa tgcagtgtgc ttaggggcca 48300 tttagggatt ctaggataca atggttttta acgtgcctta tcttaggttt cagagcagaa 48360 aacaaaatgg attcagcatt gttttttgct tgtttttgag tcacaatata ataattgatt 48420 aaaattccta ctttcttctt actctcaaag tgaccatgtt tttctctgag aagcggatgg 48480 ctgactgctc attctagaca taaatcatta atagtcataa taaaaagaat cagctactaa 48540 gtaacagctt cagtactgta ggtcagcacc tgaatatctg ggtgaccttt cctaaaatgc 48600 cgaagagtgc ttctgacagt ttgggatcga ggagccctcg tgtgatgctt catcactaat 48660 gacaaaataa atctcaagtg tcaagaagtg ctgccctgaa ttcacctcga ctttgccatc 48720 agcaatggat taggggcaac atccattagt gcaaggtaga tcccgctatt tattcactca 48780 ctgctctggg ctgacaactt aggtgtctgc ctgtttcagg aaacaaaagg tcacatagga 48840 gtatcagtaa tggcctgctg gattttctct ttagacattt actggaatct attatgatct 48900 agtctggttg ttttgatatc tgccatctga actttaaaaa caacaacaga aaaaaaaact 48960 tacagataat tttttaagag agggatcttg gaggctttgt gcaattattt tcctatgaat 49020 gttgatcaag cactgtgtga tctttgagtt cattttcagt cctgttttcc tgccatacac 49080 aatcatgttc ccatcatcta gaaatgcacg gacttaagca gtgagagtcc ctgcccctcc 49140 aggcctttct gttgctgtac aatatcaaaa gatttaatat gaacatagat caaaaattgt 49200 tttcttgttc tgttcctcta aaataccagt tccttttaga tttttatgct ttgtttattc 49260 actgtgctga tattaatata aagaagactt ttctggaaaa atatttttta aggtagttat 49320 gcttaaactt aagaagatta atacatttct cataagtctg tttttcacgc attttctgac 49380 aagacacata gtagcacttt tcttccaaaa tagcccactc ttccaatagc ataagccctt 49440 tgctatagtc tattacatct gcggctgtgg tttcaaggca atcatgtcca tattaatata 49500 cttgatgctt ttcttctgtc ttcactgatg ccctgtgctg atgcattnnn nnnnnnnnnn 49560 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 49620 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 49680 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 49740 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 49800 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 49860 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 49920 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 49980 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 50040 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 50100 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 50160 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 50220 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 50280 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 50340 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 50400 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 50460 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 50520 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 50580 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 50640 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 50700 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 50760 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 50820 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 50880 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 50940 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 51000 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 51060 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 51120 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 51180 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 51240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 51300 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 51360 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 51420 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 51480 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 51540 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 51600 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 51660 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 51720 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 51780 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 51840 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 51900 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 51960 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 52020 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 52080 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 52140 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 52200 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 52260 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 52320 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 52380 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 52440 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 52500 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 52560 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 52620 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 52680 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 52740 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 52800 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 52860 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 52920 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 52980 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctcccttgga 53040 actttcctcc gagatgcttt tacttggagg cttctcattc attattcaaa acccagtaca 53100 accatcaccc tgtatgtgaa gcttttcaga attccctaga actaattaag tagtctttcc 53160 ttttttcccc ttaagcataa tgtactcacc ttaattatag cacatgtttc attctgtggt 53220 aattactttt tgcatgtttt taatctccca ggaaaccaaa ggttcaaagc cccggcttca 53280 tattagaatt tcttggaaag cttttaagaa atactgtcat ctgaacccta tgccaaaacc 53340 ttggtgttaa gcttcacatt gcctcttgga tagagaagaa atagcaaatt atagataaag 53400 aacctctggg tgtggcctgg catcagtgtt ttgtttttct taaagctctt cagatgattc 53460 cagtgagcag ccagagtctg aaaccttgct gcactaggct gtgatctcct ggagagtgaa 53520 aactgtgtcc tattcaagtt tgtggacctc tcctccctcc ctaccctcat actttgcaag 53580 atgcctggtt tatagcagga gctcaagcaa gagctgtgga attaatgatc acctgcttca 53640 tttgttcggt cagtgattgg gattattgaa gattaggttc agtctattac cacttgtatt 53700 agtccgttct catactgcta taaagaaata cgtgagactg aataatttat aaagaaaaga 53760 ggtttaattg actcacagtt ctgcatggct ggggaggtct caagaaactt acaatcatgg 53820 cgaaaggcac ctcttcatag ggcggcagga gagagaatga gtgctgagcg aaggggggaa 53880 gccccttgta aaactctcag atctcatgag aactcactga ctatcatgag aacagcatga 53940 aggaagccac tcccatgatt caattatctc cgcctggtcc cacccttgac acatggggat 54000 tgtttcaatt caaggtgaga tctgtgggga cacagagcca aagcatatca ttcgacccca 54060 agcccctccc agatctcatg tcctcacatt tcaaaacaca atcatgccct tccaacagtc 54120 ccccaaagtc ttaattcatt tcagcattaa cccaaaggtc caagtccaaa gtctcatctg 54180 agacaaggca aatcccttct ccctatgagc ctgtaaaatc aaaagcaagt tagttacttc 54240 ctagatacaa tggggttaca ggcattgggt aaatacacag attcccaatg ggagaaattg 54300 gccaaaatga aggggctaca ggccccatgt gagtccaaaa tccagtaggt cagtagttaa 54360 accttatcat tccaaaatta ttttctttga ctccatatct cacatccagg tcacactgat 54420 acaagaggtg ggctcccaca gccttgggca gctctgcccc tgtggctttg cagggtacag 54480 ttgctgcttt catgggctgg cattgagtgt ctgcagcttt tccaggcaca tggtgcaagc 54540 tgttggcgga tctaccattc tgggttctgg aggatggtgg ccctcttctc acagctccgt 54600 taggcagtgc cacagtgggg actctgtgtg ggggcttgta tcccacattt tccttcctta 54660 ctaccttagc agagattctc catgagggct ctgcccttgc agcaaacatc tgcctggaca 54720 tccaggagtt tccatacatc cttggaaatc taggcagaga ttccccaacc ttaattcttg 54780 acttctgtgc atccccaggc ccaacaccat gtgaaagcca ccaaggcttg gggcttgcac 54840 tctctgaagc aatagcctga gctataatct ggactctttt agccatagct ggagctgaag 54900 cagctgggat tcagggcacc atgttccaag gctgcatagc agccagtgaa acaatttttc 54960 cctcctaggc ctccaggcct gtgatgggag aggctgccat gaaggtctct cacatgtcct 55020 ggagacattt tccgcattgt cttggtgatt caatggagaa atctccattg aattcagctc 55080 ctgttactta tgcaaatttc tgcaccaggc ttgaatttct ccccagaaaa tgggtttttc 55140 ttttctatca catcatcagg ctgcaagttt ttcaaacttt tatgctctgc ttcctcttga 55200 acactttgtg gcttagaaac ttcttccacc agatacccta aatcatcttt ctcaagttca 55260 aaattccaca tatctctagg gaaggggcaa aatgccacca ttctctttgc atagcaagag 55320 tgacctttac cctagttccc aagaagttct tcattttcat ttgagaccac ctcagcctgg 55380 acttcgttgt ccatatcact accagcattt tggtcaaagc cattcaacaa gtctctaaga 55440 agtcccaaac cttcccacat cttcctgtct tcttctgagc cctccaaact gttccagcaa 55500 ctggctggta cccagttcca aagtcatttc cacattttcg ggtatcttta gagcagtgct 55560 tcactaccca gtaccaattt agtgtattag tccattctca cactgctatg aaaaaatacc 55620 caagactgag taatttataa ggaaaagagg ttggccaggc atggtggctc atgcctgtaa 55680 tcacagtact tgaggaggcc aaggcgggca gatcacctga gatcaggagt tcgatcccag 55740 cctggccaac atggtgaaac cccatctcta ctaaaaatac aaaaagtagc tgggcatggt 55800 ggcacacacc tgtgatccca gctatgcagg aggctgaggc aagagaatcg cttgaatcca 55860 ggaggcggtg gttgcagtga gccaaggtta caccactgca ctccaggctg ggcaacaaag 55920 tgagaccttg tctcaaacaa acaaacaaac aaacaaagac atttaattga ctcgcagttc 55980 cacagggatg gggaggcctc aggaaactta caatcatggt ggaaggcacc tcttcacagg 56040 acagcaggag agagaatgag cactgaccaa agcgggaagc cccttataaa atcatcagat 56100 ctcatgagaa ctcactcact atcatgagaa cagaatgagg gaaccacccc tatgattcaa 56160 ttatttccac ctggtactgc ccttgacaca tggggattat tacaattcga ggtaagattt 56220 gggtggggac acagagccag agcatatcac cacttttctc cctcagcaca gaacttggag 56280 taggagaacc tcgcaaggga tggggatccc attggtgtta aactgatatg caatgtccat 56340 ggtacaccac aaggtgattc gctttaatgt tcttgattcc taagactttt aaaaaattgc 56400 ttcaaataac aagggggcct gcagagccat acatgcacaa aacactgtaa tgtctcctcc 56460 cctgtctgat gggcatgctg tacaacagcc atttgaggaa cgacattaga gaatagattg 56520 ggcttcatta ttaggcctct tcttctacag tttgctcatt ggattcagga aaactgtctt 56580 aggcccgcat gtgcttttta gtgcccaggt gaacttaggt aacaagtagc actattgctt 56640 gacatggcat tatctgccca tgtttggtct ctgcttggac cagcaaactt aaatgtcaga 56700 cttccctaag gtaaaagtca aagatatgct atagactgca tctgatattt acagttcttt 56760 ctagctaaga agagctggct tttgtggtaa atattctctc atgcaaatta gaaaggtttt 56820 cccccctttg tacatatgcc tttgatgtta ttactttctt tgcttttatt tgattttgtg 56880 aaagtccaca gatgcttttg aacacataat ggcccctact tgtggctact aaaaatagta 56940 attgaaaaag tgagggatca gttttagaag cctattaatt atgtatttaa atagtttcaa 57000 attgcaaaaa aaaaaaaaat atgtcttagg gatttagaaa attactcttt cagtgctgtt 57060 tcaggtatct tcttttcaga acttgagtta aggaaggtga aaagttgtca ttccactttt 57120 tctaccattc cagatccatc ttctttaatt cctgcttttt cttttccatc actattttat 57180 ttctccgctt ttctctttac gtgatgccaa tagtaatatt aataattgtt ggtcgggtgc 57240 agtgactcat gcctgtaatc ccagcacttt gggagctaag ggcaggtgga ttgcttgagc 57300 tcaggagtta gagaccaggc ctgggcaata tggtgaaacc ccatctctac gaaaaataca 57360 aaaaattagc tggacatggt ggcacgcacc tgtagtccca actatttggg aggctgaggt 57420 agaaggatca cttgaacctg ggagacagag gttgcagtga gctgagatca tgcccctgca 57480 ctctaacctg ggtgacagag tgagaccccc tctcaaaaat aataataata attgttgctt 57540 attggacaac tgtgtgtttt acacagtact aagcacttta tctcatttaa tctctcaaaa 57600 ccctgtgaag taggtactgt tattagcccc actatacaga ggagaaaact gaggcagcac 57660 aaggaactta agtaactcta ggtcacacag ctaggaactg gttggggaag ggttcagatc 57720 taagccatct gttttaagag cccaggctct taactatttg ctacctgacc caaagttcag 57780 agtggtaaat ggtagtagaa tgaagactaa gcatactgca tttgatttaa aaaataataa 57840 agttattttt cccccatctt aacccccatt actttaataa tggattcact tctcagcact 57900 attaatgaaa gaatttaata gaacaagtac tgagcttgag atttcatgct atcatttcta 57960 atcagacaca gaatttgtaa ccagtaatga caaaaaatag caaattgtgg ataacaagga 58020 gatgttacag cctacttagc atcttgggtg gggggcaggg acagctggag agggacagtg 58080 gtccctggag tcagccttct atggactctg atggagtctg gccaagggaa gaccacactc 58140 agccaaccat tttggggttc caggagcagg gacaacaggg cagcagctgt gaaagcttac 58200 acctcagtga ttaggtgggg agggtccata agaggccaaa aagggagaga ggcaccccac 58260 tcccaccccc atgcacacag aaaggaaagg ctgggccaac tttgcaggac tgactgaccc 58320 aacatctagc ccaaaccaag catttgacac aggcggaagc tagaagactg gcctgagatc 58380 ttaagtatag ttagagggag actagagcta gtccagggta ccttggcacc tttgtccaag 58440 gatttttcta gcataaaatg aaaaaaaaaa atgactcttt gaggaaatag gagagacgga 58500 gcttacactg atgtgtaagc tttcacagct gctgccctgt tgtccctgct cctggaaccc 58560 caaaattgtt ggctgagtgt ggtcttccct tggcttccta gaattgacct taagaggata 58620 tcattttcag tgttgccttt ggtaataaaa caaaccaatt tgcagtgact attccagtag 58680 ctgatttctt taagcacaaa gcatactttt aggggtcttc agaggcatgg tagaaacccc 58740 cttacctgac taaatcaggt ctggtaatta gttggttagt agaaaagttg gtaaatgcag 58800 gaaaacatgc aaaaatataa atatgtatgt aaatatggtg ttttaaccta taagcataca 58860 ttcgtttgat catctttgga tgcagagtga gagatttttc ctttgagttg ggctcttcaa 58920 actggagttc cccattgttt tctttgcatc actacagtca ttctgcagct ttgattttta 58980 gtcttaagtt tctttaaagt ggagtgagac cataaaaaca catgtgcctc aatctgagca 59040 caggatcctt catcttaatg atttttctca gtcttttttt ttgtattgct cacattgcaa 59100 tgattttctt ttgcaagtca tcttccccaa gtctttctta cattcacttt catagaaata 59160 gctccctttc ctttcacact cagatttcat tatttatgtg attggtgtta aattaaatgc 59220 ataattaata acaaacagaa ctggcataaa aaagttgggt acaaacatag gtaactcttc 59280 agaaacataa agtgctgctg gtggaagcag aaggaagatg ggtgagtgaa agggaaacac 59340 ttgcagtgat ggtttttggt gtgtttgtgg gaggggtggt tactttattg ggttggtgaa 59400 atgaaaactg gccaactgat gttaaaggga aaattatttg taatgcttgt taaagcatag 59460 tcaggaccat cataacaggt ctagggacca ctgcaatggg ttttgcagta taaaagagag 59520 atcaggctca actcctagta caaaaaggaa aagtgtgaat ttagagccaa ggtccagggt 59580 gggagagtta gtggatagaa aattaccaag agggatctac cgccatatca ccctgaatgc 59640 acccagtctc atcagaaaat taccaagagg aaacattagg ggtaaagaag actttgggta 59700 agttgaccta actggattct tgctgaagac agagcagggt gatcagatat tgcccagggg 59760 atctgatcag atattgaaga caaccagcca tctagagtcg gggattcgct gacttggcag 59820 gattcttgct aaaattgggc aatgcagaaa tgaacacaga agtccatact tcatagtcta 59880 gttgagaaga gtggtccaaa gactagggtt tggtcaagga gagattgtgt gtcactgggc 59940 ctctattgta gacacacctc agatttgtgg aagaaactct acctttggga aaacaaatgc 60000 acctacttct tatgaaagaa gtaataacat ctaaaaaaat gaagcattat ttgagtaaca 60060 aagctttatt taatgtcagt ccatattatt cttttcaacc atttccagaa atgtccttat 60120 cattcaacaa aaatctactt ttatttttct ttgactcaaa aattttgttt taacctaaga 60180 agatctgcat ataaaaagtg attgtgattg tcacctcatg gacattatca attgtgtaag 60240 tcaattctca cactgctgta aagaaatacg tgagacggac taatttataa agaagagagg 60300 tttaattagc tcacagttct tcaggctgta caggaagcat ggttgggggg gcctcaggaa 60360 acttacaatc atggtggaag gcaaagggga agcaggcatg tcttacatgt ctggaacaga 60420 aggaagagag agaagaggga ggtgctacac acttttaaac aaccagatct tgaaaagcaa 60480 aagggaaatc tacccccatg gtctaatcac ctcccaggtc ccacctccaa cattgggaat 60540 tacaatttga catgagattg ggcagggaca cagacccaaa ccttatcatc agtgaattcg 60600 gaatactaat acagaatgtt tttcattgaa aatcagttta taaataaatg gtgcaaggaa 60660 ttctagagta atgcattagc catgcattac tgaagtgctt aagataattt ttaaaaactg 60720 atggactaaa agttggtatg agaaaaacac tatatatcat ttcctaaagt tgtnnnnnnn 60780 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccaggtg tagtggtggg 60840 cgcctgtagt cccagctact cggggaggct gaggcaggaa gtaatggcgt gaactgggga 60900 ggcagagctt gcagtgagcc aaaatcgcgc cactgcactc tagcactcta gcctgggtga 60960 cagagcgaga ctctgtctca aaaaaaaaaa aagaaaaaag aaaaagaaaa cgagaaaaag 61020 aatgatacaa tggactttgg ggacttgtgg ggaagagtgg gaggggggca agggataaaa 61080 gactataaat atggtgcagt ttatactgct cgggtaatgg gtgcgccaaa atctcacgaa 61140 tcaccactaa agagcttact catgtaatca aaaactacct gtaccccaat aacttatgga 61200 aaaataaaaa ttaaaaaaaa gaagctgcca ggccacatct tgaatgcttt gctgcttaga 61260 aattacttcc accagatatc ctaaatcatc tctctcaagt tcaaagttcc acagatccct 61320 agagcagagg cacaatgcca ccagtctctt tgctaaagca tagcaagagt gacccttgct 61380 ccatttccca ataagttcct catttccatc tgagacctcc tcagcctgga attcactgtc 61440 cttattgaaa tcagaatttt ggtcacaacc attttaaaag cctctaggaa gcttcaaact 61500 atccctcatc ttcccatctt cttctgagcc ctccaaactg tcgaatctct gcccattacc 61560 cagttccaac actgcttccc attttttggt gtccttatag caatgcccca cttcttggta 61620 ccaattttct gtattagtcc attctcgcac tgctataaag aaatgcctga gaatgggaaa 61680 tttataaaga aaagaggttt aattggctca tagttcttca ggctgtacag gaagcatggt 61740 tggggaggcc tcaggaaact tacaatcatg gtggaaggca aagaagaagc aagcatgtct 61800 tatatgtctg gaacacaagg aagagaaggg gaaggtgtta cacactttta aacaaccaga 61860 tcttgaaaag caaaagggaa atctaccccc atggtctaat cacctcccag gtcccacctc 61920 caacattggg aattacaatt tgacatgaga tttgggcagg gacacagacc caaaccttat 61980 catcagtgaa ttcggaatac taatacagaa tgtttttcat tgaaaatcag tttataaata 62040 aatggtgcaa ggaattctag agtaatgcat tagacatgca ttactgaagt gcttaagata 62100 atttttaaaa actgatggac taaaagttgg tatgagaaaa acactatata tcatttccta 62160 aagttgttct gttttgttta aatatggttt aaaatggtgt tgggggaaaa gacatgtccc 62220 cttagggatt gtcagaaaga atctaaagca gcttagcaaa gagacagaaa tgtaaatgtg 62280 atatttatca ggggaaatga gggtatgatt aaataaaatg gaaccagcct ccatgtcaga 62340 aaatacctta atgaggaagt catnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 62400 nnnnnnnnnn nnnggtctaa tcacctccca ggtcccacct ccaacattgg gaattacaat 62460 ttgacatgag atttgggcag ggacacagac ccaaacctta tcatcagtga attcggaata 62520 ctaatacaga atgtttttca ttgaaaatca gtttataaat aaatggtgca aggaattcta 62580 gagtaatgca ttagccatgc attactgaag tgcttaagat aatttttaaa aactgatgga 62640 ctaaaagttg gtatgagaaa aacactatat atcatttcct aaagttgttc tgttttgttt 62700 aaatatggtt taaaatggtg ttgggggaaa agacatgtcc ccttagggat tgtcagaaag 62760 aatctaaagc agcttagcaa agagacagaa atgtaaatgt gatatttatc aggggaaatg 62820 agggtatgat taaataaaat ggaaccagcc tccatgtcag aaaatacctt aatgaggaag 62880 tcatacttga ggcctttgtg tcatcttctg aaagactaag agcctggact ggccaggaat 62940 ggccttcacc agaactatgg taggaagagt aagttgagtt tatttctcag gtcaggttca 63000 acctcctgaa atcccagctg aattaaagtc ttcattaaat tcatgaaagg acacaagagt 63060 cacagcctgg cctcttttaa catcgctttc ctggaattga atggaaagtc agcttctagt 63120 tctgtggaag gcaggtccca ggggaggtga gcagcttcta attccacagc tcctgcttgc 63180 ccttttgaga gctccagctc atctagcagg tcttgcctgt ttcacatctt tggagttaaa 63240 atactctgta gccttcatct cctcccctca aacatgtatt taaaaaaata caaactttaa 63300 gttaaaaatg tattagaaat gttgtctgtg ttattggaaa gcaccctcag attctaaggt 63360 atacacagat tacttggggg tcttattcaa atgcagactt tgagtcagtg ggtttggagt 63420 ggcgtctaag agtctacatt tccagtaagc tccccagggc aagggaggga gggactggtt 63480 tcactggttc atgggtcata ctttgagaag tgaggatcta aaagacctga tttcaagttc 63540 tgacttctga caactgtttt cttacttgta aaatgagagg ctttggatga agataccttt 63600 taagtctgcc cgtctgcagc atctcaaggc acagagagag agaaggctgg agattgtcta 63660 gagcttgatg tctgctctga gttttcaagg actccccgtt ataacactgt gaatatagaa 63720 aggaaaagtg aatgcaaaca gggaaggaaa tacagtttgt gtctgacaat cccagagtgg 63780 ttacaattat tcacctgatg cctttgccaa catttaaagg tttggcttta attagatcac 63840 acaatccaca aatgaacctt ctaattgtgc tgtgtttgtc tccaataagc tgttgggtag 63900 gattaattct ttccacagaa tgcccaatgg aacagtttag ttttcaatac aaactaagtg 63960 tagtcacaaa agattactac ctttagatct taaaaatatt tttaaacaga gtgcaagtaa 64020 agatacagtt tgatatgaca ttttttatac taactgtgtc agtcatggag gaatttaata 64080 ccattttgaa atctttctta tccttcggat tcccctctga tgttctcttg ttagtaaaat 64140 cttcccagag gccacgtgag aattagtagc caccttctgc ccctctgctc ctatttacac 64200 tgctgttcgg gtgcacacca catggttggg cttgagctca tttagttttg tatctgtttt 64260 tgtccttctc tgaattacga gctccttaag ccatcctgac agagtaggaa aaaaatttgg 64320 atgacttgga gcacaggtac atgagtttac actttgttct gttctgatcc tatgatccca 64380 ggaggccatt ctgccttcgt ttttgataag ccactttccc cagactgtaa atcccatatt 64440 ctcaggggtc tgaaagtcca aatgccttca gggaaaggtg gtaggaggaa tgcagaaagc 64500 agtggatggt gatagtctag ggggcagtag gaatcttggt taacctgcct tgccccaaga 64560 tctcagatgc attttcttta aacacaacag ggttgagctc aagcagaaat taacacccag 64620 ctaaagactt tgcttcacat cacctggttt ctggtttgca cataacaggg gctcccaagt 64680 tgagcttagc agaaagaaca gtggaaatat tcaaaataat taataacaag gatagccagg 64740 gaatcaaaat agcatgttta tagctctaag gtttacccta tagcatcagg ttttaaccct 64800 ttagttactg gatcaaggga aagtccagga cttcccagtg gagaggccag ggcagttgag 64860 cctggtgaaa tggaatatca gccctgagaa agacccccag gctttagtca gagaaacatc 64920 atttcacaac cctggctagc cacttactgt ctgttagatc aagttgaata atctgtctga 64980 gcctcagttt tctcatctgt aaaatgggtt aataatactc actttgcagg actgtggtga 65040 ggattaagtg aaccaacata tggaatgttc ctcaaagagt gcctggcaca aacagatgct 65100 cactcagtga aagtagtacc aggggctgtt gtttactatt atctttaaaa gataataagt 65160 ggttattgat tgaatgcaga cagcttgtct ttgtgttact catgctttct gtagtcagat 65220 aattcaggtg gaccacaaga gatcttggtt tactgttgcc atcaaggtgg cttaccccag 65280 ggctcagggg ctgggtgggg gcagttctga aagacataag gagaagggga taggcgaggc 65340 tttgttccct gggctggagc tatatgaaag actcccaagt ctttagatca cagcatccac 65400 aggccgctcc tgatctataa aagctcactt tctgatttgt aggcatttta aaagacacag 65460 atattcttat aaagatgctt agctagtgat tatcttctga gaggcatgag cggcaaactt 65520 gagcctctta tgctgtaaat aagagatctg ctacataggc atttccctgg gtcaatcctt 65580 ctgcttttgg aatccctcgt gctgcactta tacattctct aaattgtctc caggacacac 65640 actgggtctc atgacaggag gaatctccat tgagaaggat gatctgtcca tagtcagcac 65700 catagtacag agaaaaatat accctccaca cccaggcaaa ggttgaccac cctattctct 65760 gtagtaaccc agcctaaata aagacagctt ttattttaca attgctcaca acattcacca 65820 ttcactgtag ctgagtgtac tcagctatct gcaaaacagc atccctgcat tattgatgtg 65880 agggtcgtga gaccttgcca agtgtttttt ccatattgct tactcttcaa cttgaaaact 65940 gattactgtg tcctgaaggt tatattttta aaggaaattt tcctcatgca aaatgctgga 66000 atgtattttg ttccttgttc acttgctgtt acatgtgtca ttgactccca acctctgatg 66060 tgtttctttg ttttctgtgt gttgcaggca gcatagtgta cctcgggatg atggtggggg 66120 cgttcttctg gggaggactg gcagacaaag tgggaaggaa acagtctctt ctgatttgca 66180 tgtctgtcaa cggattcttt gccttccttt cttcatttgt ccaaggttat ggcttctttc 66240 tcttctgtcg cttactttct ggattcgggt aaggttttct ccttcatatt ttatagtaat 66300 gtgtttattc ttcataattc tccactctag agaaccttat tgggaggaaa ttatcccttc 66360 acacatctcc ttccatatcc tcatgtcagc cctggaaacc acaatgctgg ttcttagaaa 66420 tataattatc aacctttaaa tacaggacag aagggaacac acacctatgt tttcatagct 66480 gattattact tctagaagaa gagtattgtt ttaaatggaa tgctgtttct atggtagaac 66540 acattcacac atatatgctg cttctgaaaa tttaacataa gtaggaaata gctctcgtcc 66600 tacctgttat gaactaatta aaggtttgag ccattcaagc atgctcttaa attcaggatt 66660 agagtgtctc aaataaatgc acttttattg gtctatgtta acttggcata tttaacttgg 66720 tagaaagcct ccttgagacg taatttttag taagttcctg acttctgttg aattaacaga 66780 agttgtgcta ttacccaaat gcagtggtag agtgtagccg atgttcattt tctttaaaga 66840 gcttaaagca cttataacta tactaaatga atccataagc atagaaaaac ctaatagctg 66900 aataaggaat atatgagaaa ctttgcttca ataagaaaaa aacatagtaa aaatataaaa 66960 tttttataac gaatgggtac taggcttaat gcctgggtga caaaataatc tgtacaacaa 67020 acccccacga cacaagttta cctctataac aaatctgcac atgttcccct gaacttaaaa 67080 gttaaatata tctatatata aaatttttga ttcatgatta agaacttaga gacttttttt 67140 tcaagatgtc taacacaaaa tgaatctgta ggaataatat acaattttta aacataaatg 67200 aagaaaatga gggaaacggg gctgggttat aaactttaaa agctatcagt tgtggttgtt 67260 agtgatacat caccacaacc tacaaagcag aacacagttt tgtgaaaaga ggtcagagga 67320 ctatagcatg tctttcttaa ttatgtatag tagcatttcc cacagctcct atgctacatt 67380 ctgcacacaa tgagcactta gcaaatactt gactaagttg cacaaatttt aggagctttc 67440 cttagagtga agacagtgca tttcagtaaa ccaagatggc tggagagttt gggaaagtgt 67500 aaaatatttt gttcttgggg aaaaaaaaat gttcagaatc aatcatttgc tttgtagcaa 67560 cggagaaagg ggagaggagg ctttttctcc acaggggagc cccttggtgc tcagtttctg 67620 tgagactggc tacatacgct gctgacttga agatggattt tggattatgg actcaaaggg 67680 tttcagcaga cctaaatgtt gaatgattga atgaatgaat gaagaacaga tgttacttgg 67740 ggctattagt taaaggacct tctcagtgag taggtccata ggataagata ttcttgccac 67800 caggaaaggc caagcttcct tctccctttt attatctata ataaaaccac tagcctgtca 67860 acagaaggct aacagcataa gcactgaatt ttccagtcct ttcagtttgc aatctttttt 67920 gggaaattga agcaatatcc aaagacccaa aagtacatga ctgtggtcaa attattgtgg 67980 caattttaat tgccaatctg gattgagaca tggtcgtccc aacagactgt agagaaaaag 68040 gtccattgtg gtggctgcaa aatattccaa accaatggcc tgtcccagaa gcgaattcaa 68100 gggcaacata aaagcagaga caacttagat gtattcttga tatantcctc attcctactg 68160 cagacaaatc attcttcagc caacaggtca ggactcccgg gggctgggaa tcaagaaatc 68220 ccaggtgtgc cctactcctg cccattatca tggagtctta ggaaagtcat gcaggctctc 68280 ctgcctgctt tttcttttgt aaaagaagac attaggactt tgcattgtta ccttggagtg 68340 tgaagatcaa atgagatgat gtattgaaag tacaaggtag agaagataat gtaggataat 68400 gtttagaaac actcactcat agtttggcat ttcttatctc ctttgttcct cctcacatac 68460 attttgctaa gctgttctat gttgattgcc ttggagtgac agcaggggag gaggcagaaa 68520 gagactcctt tctggctttt taaatttcaa gtctggccca tgcaagaaac agaaaggcct 68580 aatcagggac aaaatatttg ggagaaaaca tggcaagtaa aatcaagcat aaatcaagtt 68640 aatctctata ataaatggtg agtacctagg ctggggacag ggctgagggg ggagtcatgt 68700 gagcttagtt accttagtcc aggtggcaaa atctctgcag agttttgtgg gatccagcgg 68760 ctgacaggac ctgagaaatg aagttattaa ggaaaatgag ggaagaaatc tatattggtc 68820 agcattctcc agagagagag aaccaatagg aaagaatgga tagaatagat agacaggcag 68880 atagatagat agatagatag atagatagat agatagatag atagatagat agatatgaga 68940 caggactaat taaggcaacg gctcacacaa ttatggaggc tgaggagtcc catggcaggc 69000 cttctgcaag ctggaaatgc tgggatgcca gtagcatggc tcagtataag tttgaaggcc 69060 tcagaaccag ggaagctgat aatataactc tcattccaag gctaaaggcc taataactca 69120 gggtgctgct agtgcaagtc ccaaagtcca aaggccagag aacctgggga tctgttgtcc 69180 aaggacagga gaggaaggat gtcccagctc cgagagactg caaattagaa tttcctctgc 69240 ctttttgttc tctccaggtt cacaaccgat tggatggtgc ccacccaccc ctgagggcag 69300 aacttctcca ctcagtttac caatttgcat gcccatctcc tccagaaaca ccctcacaga 69360 cgcacccaga aataatgctt tactagctgc tatagtttgg atttttgacc tttccaaatc 69420 ttacgttgaa atttgatccc cggtgttgga ggtggggcct gatgggaggt ttttaggtct 69480 tggaggtgga tctatcatga atagattaat gtgagtgagt tattgctcta ttagttctca 69540 tgagagctgg ttgtttaaaa ataggcctgg caccccccag ccctctcttt tgcttcctct 69600 ctcactgtgt gttctctgca catgccagct cccctttgcc ctctctcctg agtggaagca 69660 tcctgaggcc atcaccagat gcccagtctt ccagccaaca gaaccatagc caaataaacc 69720 ccttttcttt ataaactgcc ccacctcagg tattccttta tagcaacaaa aatggactaa 69780 gacaccagct gtctaggtat cccttaatcc agtcaagtta acatgtaaaa ttgaccattg 69840 caatattttt cttcttcacc aaagtttatg ggctatttta cttttgcttg ctttgtgaaa 69900 tgctaccttc atggcccagg gtttttctca acagtctaga gtttatctgg gaactctgtt 69960 tctgaatctc tgaggagtct catcaaagtc tctttttctc caattccatt ccaattcaac 70020 tcaaaaaaca ttgtaacacc tactctactc actggaacca tgcaaggact ttcatggata 70080 tagctgagcc ctcacctcaa gttgcttaca gtctgtacag acaggcagct cttccagtcc 70140 ccaccttcca ggaattagag cctgaaaaga gggcacaagg ggcttgggaa tctcaaagag 70200 gagggagaaa acaataataa cattggaata agaaaatttc tatttcaatg atcctcttgt 70260 tttcaaaggc atatcgtatt taaacccttc attcctatta cttattaact cagtacagaa 70320 gcagctgaag ttttatatat tctttttttt atttaaactt tctttattac aacttaagcc 70380 agagaattaa gattctcatc ctagagacct taaataaaag accatgtttt atctacttaa 70440 acaactccct ccaaccatat ctggtccagt ttttaaattg agaaaaacta cgtgaaataa 70500 cattccaaaa tgttgggaat gtttcttttg tgggcctctg gtgcacatag gcccccgcac 70560 tccacgcatt tcctaggagt acagtacttg ccatcctttc tgctttgaag cttcttctcc 70620 cagggccctc ccactcacag cttcccagag gcgtttcctg agaccacaca cccagtgggt 70680 ctcagagagt ttcctgtttt gtctccttgg cagcacttac aattgatgcc tgaaaatatt 70740 tacttcttat gttcttcttt ctctcctcca ccagcatgta aattccaaag ggctgggact 70800 gtcgtcttgt tcaacattgc ttaatcgccc atggcagagt gggcatgggt attgtaaaag 70860 acagtggagt tcaagcagca accagcatag tctgacttgc agccatctgt gtcattggct 70920 agttaatcat gaagtgaaat agataggaag cctactcaat tcttacttga tctgtataag 70980 tagaaaactt atatgactag tgaatgaaaa tctaacttga gtcatgtccc ctccatcatt 71040 cccagactgg agctgcttta cagacccaga accaactgaa tgaaggggag gctgggcctc 71100 cttgaagaag gatctttgta cccagctaaa tatttatact gttagtcttt ctcccagctt 71160 ccccaaaggg actgaaggcc ttttaccagg atgagtgtgc atgggagaaa ggaaataatc 71220 agacttctca gggcttgtgg acactggctc taaactgaca ctaattccag gagacacaaa 71280 atgtcactgt ggtcaaccag tcagagcagg ggcttatgga aggcaggtaa tcaatggtgt 71340 tttagctcag gtctgtttca cagtgggccc agtgagtcag gcactgaatc catcccgtgc 71400 ctgattccac agttctggac tgcataattg gaacagacct actcagcagc tggcagaatt 71460 cccacattgg tacagaatcc ccacatttaa ttggtacagg gcctgtccat cagctccatt 71520 tactaggaat tttagaacaa agagaagttc tagttcagag agtccaaact tttcattttg 71580 cagattgagg tgcagtggtg gaaccaggaa taactcagct aggacttaaa ttgcaattaa 71640 agtcatttta aacttgcaac tctgtaaata agtaaaatgt acattctgaa tccaaggttt 71700 acctgtctgt gtgttgattt tatcaaaaat gctacagatt tccagttgct gtgtcttatt 71760 tcattgctga agcatcatta gcagcactgt ctctgcaaat aaaggggact ggaaggagtt 71820 cttttttatt ttattttttg ttttattttg ttttttgaga tgggatctca ctctgtcacc 71880 caggctggaa tgcagtggtg cgatcatggc tcactgcagc cttgacctcc tgggctcaag 71940 tgatcctcct acctcagcat cctgagtagc tgggaccaca gatgcatgcc accacaccca 72000 gctaactttt aaaaatgttt tgtacaaaca aggtctccct atgttgccaa ggctggtttc 72060 aaactcctgg attcaagtaa tcctcctagc cttggctttc caaagtgctg ggattgcagg 72120 catgagctgc tgcacctggc caagctcttg ttttaaactg agatctactt gggtttatat 72180 ttttattgtc tccaaatgtg ctttcctctt aattcatcaa accaagtcat gagcatatct 72240 tcttagaaac acaaatatgt ccagctgaca tattgtatat gactttaaat gtttattcgg 72300 tacaatgaat gattttcctg cctggggaca acagcgttgt gtgggaagga ggactaggac 72360 atcaccaatt actcatctgt ctatcacttt cctaaacgaa tctcacacca agaaacagac 72420 actttcctcc cctcttttgt tatagaaaat tagaaagatg ggaaatggag ttggcaccat 72480 tgattcattt tcatttgacc cagaattaaa ttatttgtag ccagtcactg ttaattatgg 72540 gaatgatagt actttggatg ctaggcaata tcagagtcgg gagcttctca ctgagggcct 72600 ggcactgcta agcaggaagg tcaggagaaa aatataatag caacagaaat taaaatgaaa 72660 tttcaacgtg tctgtatatc gtaaatgaaa gaatagtaat ttcttcctct gtgaatagag 72720 cctaccaaat gtgtacttca tagctatttg caatattcat tcattagctg tattgagtta 72780 tagtaagaat accttggctt tgtagaaatc atcaccatta actggaatta ttcagaatgc 72840 tttatatgta gcatcccatt tatttccaca attgttttaa ataatttgtc atgtaaaaat 72900 tgtgtgcctt ttttattaga aaattgaggc acagaggccg ggtacggtgg ctcatgcctg 72960 taatcccagc actttgggag gctgaggtgg gcggatcacg aggtcaggag atcgagacca 73020 tcctggctaa catggtgaaa ccccgtctct actaaaaata caaaaaatta gccaggcgtg 73080 gtggcaggcg cctgtagtcc cagccactcg ggaggctgag gcaggagaat ggcgaacctg 73140 ggaggcggag cttgcagtga gctgagctcg cgccactgca ctccagcctg ggcgacagag 73200 cgagactcca tctcaaaaaa aaaaaaaaaa aaaagaaaga aaaagaaaat tgaggcacgg 73260 attgattatt gcttccaaat attcaacaga cacccagtct tctgaatttt ggtctgattc 73320 tgagctatga ggtttttccc ttcaaagtag tctttttctt aaaataatca tattggaaaa 73380 atgcagtcat ttaaaattgt ttaagacttt taaaatgtat tttatcaaac acctctatag 73440 tgcttgccgc gtgagagatg ctgtgttcag cactttatat tagtaaatat tgactctcat 73500 cagtgacccg atgagaaaga tgctattcat tattcatata tcttaactgt atgtcataaa 73560 tgaggaagtt gaagtacaga gaggttaagt tcctatctga ggtcacacaa taagtaatgt 73620 aatcaggttt catacccagg cagtctggct ccagggccca tgctcataac catcaggctg 73680 tgctgtgctt cagactcaac cagaaatgtc tctgatttca tatggcacaa cgtatacatg 73740 atatgtgctc atacgtgttt gaacagatca gtccaaaaat atctctggcc ccagagcatc 73800 agaagggaaa gaccttcccc aatatgtaaa atgaggcaac agtcaagatt atctttaatc 73860 caaaaataat ataaaaccca aattgtaaac agtgtgtcag tgttttctta gagctgcgtt 73920 gtccaacatg gtaaccacta gcttcatgta gctaccaagc acttgaaatg tgactagtcc 73980 aagttgagat gtgttgtagg tataaaatac atagcagtgg atttcaaaga cctagaacat 74040 ataaaagaat gcaaaatatc tcaataattt tacattgatt acacgttaaa ataatatttt 74100 acatatattg ggttaaatga ctatatatta aaattaactt tacgtgattg ttttgtttta 74160 atgtctcttc tggaaaaatt taaaattaca tacgtggctc acattttcta ttggaaagca 74220 ctgtcttagg caatgctttt tactttccaa caaaaaggct gaccttttgg acacattttg 74280 tttcccagta taccctttag caaaggtact attgatcaca aaggagaagg aacagaacaa 74340 aagatcttcc agacccacag tgtataattg tgaaaaatta gccatatgca ctctttagaa 74400 ttcactctct ggagcccaca gcaggaattt gtgtgcttca tgcctgcacc tccaacaact 74460 ggtgatgtgc ctggcccaga gtttgattgg gtgagggggt ggatagaggg ataatgggtg 74520 gataatgggt aaatgaaggg tgggtgggag gatgaagtgc aggaatggat gaggaaatgt 74580 atacagcaag aggctagata catctgcatg ttctatcatt aattatagaa catcataatg 74640 atcatcacca tctttattca tggcaccttt agatgctatg ggaaggcatt cttccaactc 74700 tatcaatagc taattttttt tcaagacagt attaaagtct tgatccatga ctaccccact 74760 tccctcagtg gtactaagtc tgctactgtg gatgcataga cctccacagt tttagggggt 74820 taggccacca atctgatagc aaaccagggc taagggagtt gaattgcagc tgttttcttg 74880 gcaatgcagt tttccccaca aattatatac ttaaagatca atatttttta aataaccaaa 74940 ttttactact cattattgct attcatagtt tacataatat ttagaaaatg acaacttcat 75000 atatcgaaga atattatttt tcaaattgtg gctttggaaa ttgaagacta aagctaccca 75060 aatcatccat cctttggcat tgccactggg caaaggaaat tcttgttctt gatttgatga 75120 tgagttaggt taccagtatg tgtttttctc ccatttctat ctttatcact gatagcaaac 75180 atgtgccttc tgatctatat agcaacctta ttttctgtgc caacctagcc tattcagaaa 75240 gagtgttagg tgtgtgctct ttcatctaag cctctgagca atctccccaa ggtctgtgat 75300 actctattta ctttcataaa gtggaagaga ctgaaaggtt catctttgga ctgctgctct 75360 aatcccttat ttctgggtcc aatttataac ataataaatt ggctctgaat tgctttgcaa 75420 tttgcctgac agttcagtgt cttctctcct ttttgttgat tccgagtaag aattaaaaga 75480 atatttttca tgcaagttat ttgtaattgt gattcctgac ataggccaga ccattcacta 75540 atctttttat aatcctataa tacttgtctt ataaaagctg ccagagtcag ctcttggtag 75600 actctgccct ctgtttatta tttgtattga acatgtgact ttacaatgat tattcaactt 75660 tctccttaat tatgttcttg cagaatgtcc catcttccag tggaaatggg aaatggaaga 75720 ttgaggtaaa gggctgcttg acaattatat ggtgaaataa gttagaatga attattcttt 75780 gttacatact agctagaaaa ttaaagcaga atagaattac tcagtagaat cccttctcaa 75840 gacaaagaat catcaaacat tccataattt ctctctaaaa tgccatgctt caagccattt 75900 taagtacaat tactcttttt atctataacc tccctctttc attgaagaaa cctaaacccc 75960 tccccacctt tgcctgaaac agcgtacatc agtgtgatga agcacagcaa caaacataat 76020 ccccactggg ttgccagctg aactggagta agacgaggcg tggctgtgaa attcacagtc 76080 aggcttccca gtttcccaat gaggtttgtg catacaggaa gacttggcat actctgtcat 76140 cctgttgtgc taaactggtt taacttgtgt gcctcacatt tcccagcaga ggagaaattt 76200 ggtctgtaat gctaacacat tctttgcctt gcacaatatg ccacttgtga atttgcctta 76260 gattattggg gaaaataaat gaatatattt catgatattt accagatgcc tagtattgag 76320 tcatgtgcag acctattgag gtgttgtgcg tgtgtgtgtg tgtgtgtgta tgtgtgtgtg 76380 tgtctgtgtc tgcttgcctg tgcattgggg agattctgat tcattctaga aatgtgttta 76440 atacagaatg ttttagtttc tttatatttg tataattaaa gttgcaaaaa cctagcacta 76500 atatttctta aggaataact ccaactctga aattccaaat attaacattc tatttcattt 76560 gtataaatta agttattttg aatctggcta atctcacacc tacgtagagt gtgaatttct 76620 attattattc cttctgtgcc tcttattacc aaggataatt aagatttgac atttttgaca 76680 gctaataaaa tagacttaat atccaatact gagaaaaatt ctttataagc cactctgatc 76740 tcattgcttc atttatgtaa atgttccatt aaatataact tgaaaaaaat gaacttgaac 76800 agatttcaac agtagctaac tgatcattct ataacgaaca ctaaggtctc cacgtaaact 76860 ggatttaagg atgtgttgcc catcagttaa tgtaaaagat atttgtgtct tatattatgg 76920 acttttatat aaaaccaatt atattttcca cattagcccc ccactgcaaa actttcccaa 76980 ccctagcgct attaatattt tggactggat agttcttttt tgtaaggagc tgttctgtga 77040 gttgtagggt gtttagcagc gtctctggcc tctaccctct agatgtcagc agcagccccc 77100 aaggtatgac aacctaaagg tctccagaca ttgccagata tcttcaggga gcaaagagga 77160 gcaaaatcag ctctatttaa gacctactga tacatgggaa tttaaggact ttcatttaca 77220 tttttttaat ttcttcatat attttcagaa attgtatctt agtgtaataa acaaacaaaa 77280 aacaacaaat aaggaaattt ttaacccctc acatgcaata ttgaggtaga atcataatgg 77340 gaaacttctt tttaatttat aagatttggg ggttggggag tggcagattt ttcttatctt 77400 caaaacttac cagaggaacc aaattatgtt gtaagacctg aaagcccttg ctttatctct 77460 tccctaaaca tttttctgat gatcattata gcatctgcaa cctttttctg aaatgctcac 77520 aggtgttata gaagctcctg gaatgcccag tgggattgcc cagtgtcccc tgagcagttc 77580 caagggagct ctctccacaa caaggaccgt caggtcattt ggtcaagggg tgaccacagc 77640 aactaaggca tagagcaact caggaaccct ttgggggctc cattgctgtc aaaactttca 77700 gtctagcacc ctcaacttct attatggtca tattggttca gaaagtgagg tgtatctttc 77760 tattcactgg agatctttaa atcatcagaa cagattactc caggagactt ggtatgccaa 77820 ctacaacatt gaagagtctg ggaaattcag ttcttttgcg taaacactta gcacatgtcc 77880 actgggccag ttaatgggga ctcaagaatg aatatgataa agtccctacc ttcaaggggt 77940 ttatggggtg tgaaacacta gtaatagtaa tagtttcttc tctgtttaaa ggacatcaca 78000 gtaggacaag gagtaatttt ttccccaaaa tatatcatca gcccacaata agaaatgtga 78060 atgcttggat tgttttatat atcgctgagt ctaaataact ttgcaaagga tttaaataga 78120 agccttctct aattcttcag actcacttat cccttagtat tccttagcac ttccttgcag 78180 cctgctttac aaacaagtat tgagagacat ccagaaaaaa taaccacgtg aaaaagaggc 78240 attgaataaa aaccagccag attgcctaga agcccctaca agttggtgtt ttcttatgta 78300 acaatgattt gttgaaaact aacaggcaaa tctacagtct tccacttgaa attatagcca 78360 cactgcaaaa aaaaaaaaag aaagaaagaa attatagcca caccgaagta cctttatctt 78420 ggtccagagg gtctggcata tcctgacata caatagtgcc ttcatgaagc ttaagttata 78480 gctattaaga ggatgtccag agtcctgatg aaacattaat gaggaagtga aaagtcagta 78540 aaaactagga ctatttttat gcattgagaa gttaagctgg gattttatgg tgcagagaaa 78600 cggattcttc acatcaaatg aagaaaagga gtagtattaa attatttgtg acacagatat 78660 tttatttcat actaggattg tgtgttataa tgaaaccact tataatctgt tcaaaatatt 78720 gttgagtttt aaaattcaac tagcttacta taccaataag catcacacat ttgttttttg 78780 tcatggttaa tatgtgatat ttgttttcag ggcttttaat gatcacagat agcaagcaag 78840 catgcagctt accccttgaa agggcctcac tctgtcaccc aggctggagt gcagtggccc 78900 tttaaggctc actacagcct caaactcctg gattcaagtg atcttcagcc tcccagtggt 78960 ctttgtagac agcctggtgg agtctcatgg cacagaagat taattaaatg atgtctttca 79020 attttactat ctgcattcca tcaaaaaata aaaataaaat aaaataaaat tcaactagct 79080 atccattgta tgagctcctc aagaagccca ggcaagagaa ttgtatgagt ctaatacggt 79140 gcagtataga gccagctctg agcccccacc acatgcattt gtttcttgtt tgagggtttg 79200 gttatggact tggacttggt tggctgcctc tgtgtgtgat ggtgctaaat ctgaagacag 79260 gttgagtcac acctctaaga attttaggta gaaccactgc tgtcatagcc cagagaaact 79320 gatatagatg cctaaagttt gtgaatttta aactctttga acaggaactc ccactaagaa 79380 acacactgtg acccattata tattttatat atgtatatta tacattacaa atacatttat 79440 aacacaaatt tcatgaaatg atacttatgc ttatccttat tacttaggtt gcacttaaat 79500 atttttatca ctatctcatt ttttaaaatg ctctttaccg tttatagcta ctaaactaaa 79560 ttttgcaacc catagattga aaagcaccag cctaagtggt cttcccatgt ccactgatgc 79620 gtaactatgg catcacttta aacagaggca aatacatgaa atggtgccat attttcacct 79680 accagttgat gcaggatgac attgtaaatc ccgtggttta tttggtacac tagtttgctg 79740 ccaaaatgac ctttccctaa attgcatttg ttaagaacac tgccaatact tctttatcat 79800 atcaaaggct ctaagaaaat cagttattgc taaataaata aacatgcttt agtccttgac 79860 atcaaatttg catgaaatgt caatagcaat ttaccttaca gaaatctgaa caaaaatgaa 79920 tcagtgcaca cttcctaact ctattagatt ttgtgtcatc tcgacaatgt acttatgttg 79980 tgtgtctttt taaacaaata ctttaaaatt cacatattgc ttggagtgat agtaatattt 80040 ttctttacta ccttgctttt tgaggcagtg gttcctaaag ttttagggcc aagacctatt 80100 tgaaattctg atgaaatcta tggcccatct cctaaaaatg agcactattt tgattccata 80160 aaccctcatt tgagcaccct taccctgggt agaggtcact gtttctgtca tctccccaga 80220 gctcagtcag cagcttgtat aactagctcc tactgaaagg aagcattata ttccattcta 80280 ttgatatcta aaccaccagc tccttatttt attctacaag tgtccctttc atgtttactt 80340 atttagcagt aatatctact ggattgggac tagagctaat cttggaaatg accccgtgag 80400 tcatttcaac taatgtttgt caacacatta acctaattta gtgctgctgt ggggtagcag 80460 tgagtaaata cagattgcag acagtaccct agaggtgttt gaagctgttt ccaaagtccc 80520 cacatcagga atgggacatg gcacagcatc agtttttaaa aacaagttaa ttaaaatatt 80580 cactagatta taggaaatca atgtaagttt tgataaatat cacacaaaac acaaccatga 80640 gctgaaaagc caacggaatc tcatgttaag ctctatcaac cacctgagac aaacaccact 80700 gccacctctt ggtaacatag cttaattgca ggttcaactt tttggaggca taataaacct 80760 atattaaata cagagactat aatttttaca ttgtaaaata atagaaaata tatgtttgtt 80820 gatatgctgg gatagggaga aagcatgtgt tgttagagtt tgctttggct ctgccctttg 80880 cgtctcactg gagctgtcca caccctgatt ttcatgtatt ctctgtacct cttggcagga 80940 ttggaggagc catacccact gtgttctcgt actttgctga agtcctggcc cgggaaaagc 81000 ggggcgaaca cttgagctgg ctctgcatgt tctggatgat cggtggcatc tacgcctctg 81060 ccatggcctg ggccatcatc ccgcactacg gtaagaggct ggccttgccc cagctgggca 81120 gtcacttcat tttgcttcag gctccattcc catcttcttc ctctcttcta agggccactt 81180 tgagaaaaac tactcatcat catgtttctc cctttcatag tgtccaggag tttttcccct 81240 ctgtgtagag atctgtataa agtaagactg tatcaaatgc ccttctggtt atatgaatgt 81300 aatgggggga aaatattttc ctctgctatg ctttcacaag atttctggtc accaaaatgt 81360 gtggggtgtt ttctcccact gaccaattct ccaacagctg ggtgtgctac aatttaactc 81420 aattctgaca ctatgttact tggggttgga gtcaaatggc acagattaag ggctcagtcc 81480 cacaagactg ccccccattt cagaggccaa ttgtaagtcc aggcctctgg aacttctgac 81540 tgaccagcta taaatgggag gttcctatac cccccaccct caggtttgat catttgctag 81600 aatggctcac agaactcagg gaaacaactc tcattcacca gtttatggca aaggatatgg 81660 caaaggatgt agatgaacag ccaggtgaag agaaacacag ggcaaggtat gtgggaagag 81720 gtgaggggct ttcatgagtt ctccgcctca ccaccatccc agtgcctcca catgttcaac 81780 aacccaaaag ctctccaaac tccatagttc agggattttt atagaggttt ctataggcat 81840 gattgattag gtatgattga ttattaactc aatccccagc ccctctcccc ttcctggagg 81900 atgggggtgg agctgaaagc tccaagctta tgatcatggc ttggtctttc tggtgaccag 81960 tcccactcag gagcccacca acagttgtct cgttagaata aaagatgctc ctattaccca 82020 ggaaattccc aggaaataga agtgtcagga accatgaatg acgacgaaca catatgtttc 82080 ttattataat cacagcatca tagtgtacta ttgcatttta ctgtgtttaa agtaatctct 82140 ttcatttgtt tcctctgtgc tatcaatgct ggggattcct ctcccctctg cctctcctac 82200 cctaagtaga ggtgggagga tacacagatc agcagcagca tataagacaa catgacttat 82260 agttgttctt gggactgcca tacatatcaa acacacagaa gcacctttac tcaactcttg 82320 tgggctcagt ccaaagctca gctccacatc agccccacct cttctggcaa ctcagaactg 82380 gaatgggagc aggaaaactc ctgttgtatg gcctgttgaa ctgcagaact ttagaggtga 82440 ctccacctgt ccacactccc agatcccctc ctcagtttct cctaagctct tactgtttct 82500 gctgcctcag aaacctgtac atgttgcatg tttctgagca atacccctca gaagccaaaa 82560 gctccaagtt gcttcatcta taagcagtga aatgcatgga aaggtcaatt gaatccagaa 82620 atcaaattca atatatcagg aaaggggaga gaagtgttct ggttgcgtgt gtgtgtgtgt 82680 gtgtgtgtgt gtgtgtgtac atgcacacac acgtgcacat gtgcccaaac aggctaaagt 82740 ttgcaaaata ggaataccgt ataaattaaa accacatgac tgtcagtaaa ctcactctct 82800 gctctaatct aaaaaattag tatttcaaaa actcattctt tgctcatttt ttatttctct 82860 gcttttgttt tagaacatgg taatatttca ttataaaaca cagttctgtt agggaacaac 82920 cttggtgaaa ttgactcatt gctaaaatca gacatagact ttagtattta gatgtcttta 82980 acttgttttt ttaactgagt tttaacatgc tgagttacaa aatcatgtca cacgctttct 83040 tttgatgaac ctgaaaagtt acatgaaaac tgaagtcgta tttaacaaca acaaaagcaa 83100 gatataagga attggttact tggcctgaag agaacttctt tgctgaacat ttacattgag 83160 ttcagggtgc caagctcaag tggtgggttt ttcattttta aactcttagg tcacttatgg 83220 gccagagatg ccctcataca ggcaggtccc catatccacc atgagacttg tcttctgcca 83280 ccatccaagg aattcaagat tttttttttt taatttgaga tctcaggacc tcagccaccc 83340 tcagaacctt cctcggtaga gcagcatcta actcatgccc cactcaactc actggttcaa 83400 gtggaaaagc cactcaggaa ttgctttata ggaactatgt ttaagaaggt gaataggaaa 83460 tgcagagtcc agctttaccc atcattgctc cagagccact gctagtctat ccattcatac 83520 tctttgtgca tattcaaata agggctgctc caggtaggta gccagaagtg ctgccccact 83580 tatcatctct gccaactact cacagagggc tcaacctggc aaatttatac agcagctgaa 83640 gtgctttttt ttctctctct ctctcttttt cttttttttt ttttaatacc ttcttagccg 83700 gccttcaggc cagccttgtt ctcttttcag cctctgatga gagccttgct ccttggctct 83760 cgctgagctt gctctctgga gccccttgtc tccctggtta atggctgtct ctttcctggt 83820 cctcccttcc catcatatcg ctcagaattt cacctgctgg ccttcttaaa cacgtaactc 83880 acactggctt ctgcagccta cgtgtgtccg tgtgtccacc aagcccgggc atgactgttt 83940 ttatttttag ttttttttct cactaggaat tcagttcctc tgactctgcc tttcacacat 84000 ccctgccctt gaactctgga tggttccaga ttttctctcc tgccttgtca ccggtcagtc 84060 agtggccaac ccacaaagaa taagcatgat caccactttc ctttttccaa agtacactta 84120 agatacttct tgtttatagc tacaagggct aattaattca tatataacta gaatgaggga 84180 aagttgggaa tcataaacaa tgcatgaaaa gttacaaaaa acaatgacga cacaacaaaa 84240 ttaaatgtcg gaggctgaca tcaactttta tgttgacaac accttgagaa ataatgacca 84300 ggttacgaaa tgagtaatgt caatatgaaa accttatgcc catgctttac taaaggcttt 84360 ccatggccag aagtgtggca aaaaaaacat atatttcatt caattctgtc tgcaaattaa 84420 aattaaaaat gaaaagcttt aaaaataact ttgctgaggt cattgttgta ctcttgcaat 84480 ttgaagagtt ttaatttttg ttttaaagtt taatttttaa aatcctgtgc tgaggcttct 84540 ggagtgctaa tgacattcta tatcttgagc tgggtggagg tttttaatga atttttgaat 84600 tgtaaaattt gttaaactga gtgcttaata tttgtgcatg ttactatatg aatgttacac 84660 tttttcacaa acacttttta gtaagtttac cacctccccc ataccctgaa aacactcatg 84720 cttggattta ccccacatca atcaaatcag attctctgga caagagacac agcattgaga 84780 gtttttattc ttattttttt cagctttatt gaggtatgac tgacaagtaa aaattatata 84840 catttaatat gtacaacttg aaggtttgat atatgtgtac attgtgaaat gattaccaca 84900 atcgagctaa ttaacatctt tcatgtgtgt atgatgagaa cacttactct cagcaagttt 84960 caagtataca gtacagtaca atattattaa ctatagttac catgctgaat attagatctc 85020 cagaacttat catcttataa ccaaaagttt gtgccctttg accaacatct ccccccatcc 85080 ctcaattcct ggtaaccact cttaactact ctctctttct atgagttcaa cttttcagat 85140 ttcacatatc ggtgagtttg tgcaatattt gtcttttcat gtctggccta tttcacttaa 85200 cataatgcct tccaggttca ttcatgttgt tacaaaggga aggatttctt tctttttaaa 85260 ggctgaacaa aattccaata tgtgcatata tgtgtgtgtg tatgtatgta cataagtata 85320 cacatacagt tttaaagctt tactgttttt acagttttat agttctgaac ttacatttaa 85380 gtctttaatc cattttaagt tgatttttat ctgtggtgca aaaggaggtt ccaatttcat 85440 tcttttgcat atgaatatcc agctttccca acaccattta ttgaagagac tatcctttcc 85500 ttatggggta ctcttggtgc ctttatcaaa gatcagttga ctgtataagc atgggtttat 85560 ttctgggctc tctattctgt tccgttggtc tacatatctg tgtttatgcc agtactatgc 85620 tatttcaatt actatagctt tgtaatataa tttgaaatca ggaagtctcc agctttcttc 85680 ttcttgctca aggttacttt agctactcag ggtcttttgt tgttccatac agattttagg 85740 attgtttctt ctatttctgt gagaaataac attggaactt tgatagggat tacattgact 85800 ctgtagatct gtttgagtag cattgacatt ttgacaatat aattctttca atccatgaat 85860 acaatatatc ttgccattta tttgccatct tccatttctt tcatcagagt tttatagttt 85920 tcaacataca aatcttttac cttctgtgtt aaatttattc ctagtttatt ctttttgatg 85980 ctattgtaag taggattgtt tcaataattt cttttccaat acttcattat tactgtgtag 86040 aaacacaact aatttttgtc tcttgatttt gtatgctgtc acgttgctga actcatgtat 86100 tctaacattt tttggtgaca tctttagaat tttctatatg taagatcatg tcacctgcag 86160 aaacatactc ttgttttact tctttctttc tgattttgat tccctttctt tctttttttg 86220 cctaattgct ctggctagga ctttcagtac tatcctgaat agaaatgaca agagtcttcc 86280 tagtcttaga gggaaagctt ttagattttc accatcgagt atgttagctg ggagcttgtc 86340 attgatggcc ttgattatgt taaggtacat caatagtttt taaagcttct gggtggttct 86400 aatgtgcagc caggtttgag attcttcagc ttcctgtcca tgaaaggccc aatgggtgac 86460 ctcagaagtt tgtttttctg ctgggaccct agaacttgat ggtatgtatt gggtgggagt 86520 ggggggatgt gaatgagaat aaggagaaca gtgagcctga tctgttcagt gtttgagttt 86580 ccgtattgtt ggtttcatcc acgcaaaaac actttcctgt ccatctggtt tagaatcaca 86640 cttaaaaagt aactttgaca tctagatatc tcttcttttc agtgatgcat cttttaactt 86700 tacctaaaat agacaagggg ctaaaagtct atccactttc cctgaagcct ctgaaaaaac 86760 taaaggctta tatctctcct ctgaaatctg cgttgtataa atgatgccac agtccacaga 86820 aacaagagcg ggtccgcccc tgctgctctg tcctctttca ctgaggaggc ctttgagtgc 86880 ccaccactcc ctgtgcattg agtttgcacc ctgcagagaa tgaacacagg agtgtaataa 86940 agtgactcag agccaggacc tcagccttct catattagta atgggctttg ctctggagag 87000 aaggaatgga taattggcat ccacattgca agtgtatttc aaatgataaa taacacagag 87060 gctcgtggac aaaggtgttc gctgtttctg ggtgtcgccg tttagtaatt agggcagagc 87120 agtcagttca caaagcctga gcctgatgct gtgagactgg aattctggcc cctgacaagc 87180 ctctgatgca aagtagacct tgagtgggtc actaaggatt ctggaaggaa atctggaagg 87240 attcttgtga agttagacac tggaataagg gattggattg ctctaagggc tagtgagtta 87300 agacatgggc tgtggccagg agtttttggt ggacagtgga gtccactcag cctgctgaga 87360 cctagctttt gtaccagtgt ggaataatga tggtgctttc tcttcatatc actgctatca 87420 gagttgaaat tttccccgga gacagatggt tgctaacatc tgaaggttcc caacatgaat 87480 ggacctcacc caatgctgac aagaccttag gaggcagagt gcttggctgt ggtcacacca 87540 gcaccttccc tgccaccact ccattcagga acttctgggc catctgtgtc tcccccatgg 87600 caaagagcag tccactagaa caagggagga ggaagagcaa caaactgaga aggaaatgcc 87660 cttgcctcaa aaatcagtgc ctgtaagagc tgaaaatagg tatttagagt tggcttggcc 87720 gggtgcagtg cctcacacct gtaaacccag tgctttggga ggctgaggca ggagaattgc 87780 ctaaagccag gagttcgaga aaatttggct cttcttgacc taacacccgt tctcaagcct 87840 aggccttctg acccatagtg ggaattggct actggttcag aaataaataa gcagtatctt 87900 caagaacaga taggtcaggt gtaaaggttc tctttcagcc ttcctgccct tcctttctct 87960 ctccacactc tgtaataatc aggacacatc agcatttaat ggaagcccca gtacctcagg 88020 ggaaatggga acagagagct gaatcacagg cactttctat gttggctgag agctttcatt 88080 aggcccaggc tttggaattg ctgctagact gcggcactgt ccgctccatt aaaccctctg 88140 tcatcatttt cccttttaaa ttcatgtgcg catcagatga ggtgttctcc cctgctgtgt 88200 gcattacagt gtgtagcaaa ggtgttcaat tagctaaatg ggaaagttct tgttttagcc 88260 cgaaaggcca cagctctgaa agcccacagc acactgggcc tcatctagcc cccagaatag 88320 ccccatggcc tctatctggc tagccctgca ctgcagatgt tatagggtca aacttggggc 88380 tcccataaac gtcagactta ccccacccta cgtctccagc catgatgcga tgtccaggct 88440 taaaaataca tatattccca aggttgtcca aattaccaaa acatccctac ttcctttggc 88500 tggcaggaga ggcaatagct atctaattga agaaggaagc agggaaggat tcctgattcc 88560 tcaaccagac agatattcaa caagaaggaa gaagaaagtc agcttccttg ttcacaagaa 88620 ggaatgcact gtggccaagg gaaagaggta taaagtgaga ggtattttga gtctcagacc 88680 tgctacttct gtgagctctg tcaaagcttt tcagtctgat ccagcccact ctcctagtca 88740 gcattgtagt aaggattaag caaaataatc ttgttagaat acccagtgtg tatttgatgc 88800 tcaaaaatgg tagctcttgt tgctatcagg tcatcctaca tgggataccc agattgccag 88860 agcagcccaa atgtgagcta catgaaaaaa gagggagaag gaactaaaaa gcataaaccc 88920 tgcctaaagt ttgagattag agaagtcaga gaaaagaaaa aaaaaaggat agaagctaag 88980 ataaaagaga accaaggtat taaaacaata ctgtaaaatc ttgatgactt tttacccaat 89040 aaatcagaat cctccatttt tctgttgggt aatttaatat tcaaggggca tgtactatat 89100 aaagacccat tttccaaggc catggtgcta tcgttcccag atagaaataa cgagagtctg 89160 acaactctaa cctgtgagca tcaactacca gctgattctg cgggttatat gggcagcacc 89220 agagccaagc tctatcgcag caccaagatg tcagagatcc tggagacagg cagtcagtgc 89280 aattgttaac agcatgggct tagagggaag gccctacttc caagccacct ctgccctctc 89340 tagctttgtc atctttagga cagtaaccta ggctctctgg tcctctgttt cctcatctat 89400 aaaacaggga taataatagt atgacctaat acaattgctg tacgatcaca ttactgggta 89460 tatacccaaa ggattataaa tcattctacc ataaagacac atgcacatgt atgtttattg 89520 cagcactatt tataatagca aagacttgga acacacccaa atgcccatca atggtagact 89580 agataaagaa aatgtggcat atatacaaca tggaatacta tgcagccata aaaaataatg 89640 agctcatgtc ctttgcaggg gcatggatga agctagaaac catcattctc agcatactaa 89700 cacaggaaca gaaaaccaaa taccacatgt tctcactcat aagtgggagt tgaacaatga 89760 gaacacatgg acacagggag gggaacatca cacaccaagg cctgttggtt gagggaggca 89820 aggggaggga gagcattagg acctaatgca tgtggggctt taaacctaga tgatgggttg 89880 ataggtgcag caaatcacca tggcatatgt ataactatat aacaaacctg tacattccac 89940 acacatatcc cagaactcaa agtaaaataa aataaaataa tacaattgct gtattaaagg 90000 atggcataca aaaagtgcct gacacagtgc ctggcacaaa tgttcattaa gtgtagtctt 90060 tgcacagtgc ctggcacaaa tgttcattaa gtgtagtctc gtattttcat atttgggact 90120 gaagtttggg tcagggagga agaattaaaa agaggaatat ttaaaagatt gtatttacct 90180 tttcctaacc atgttttctc aatggttcac ctgtcatttg aagttgtgga gatggataag 90240 aaagattaga agtacaggaa cagaacttgt aaggcatgcc aaagtgtcct gtgcccacta 90300 ctctagggtg tcagggcagg cctcacatga taactggggt tcctctcagc agcccagcca 90360 ggacaggcag gtgacaggtg gcccagagcc ctgtcctctc atccttgcca tatttcactg 90420 agtgttacag ccctgatgat tttatgctca agaggcagag taagacgtga cagagggaag 90480 tgatttggta cattgactta taccagtatt atttcttaat ttgttaattt aatttgttaa 90540 ttttacgatc aatcttatat tttatctcaa cgaaagaacc cttggcttcc gatttccttc 90600 agtggtggtt cacttttccc catgcaataa taagatgcat cagtcaaata aacattgatg 90660 atatagtctt taagggctta aaaagtagat gtgaaatttt aaaaaaatta tcatgtgttt 90720 aagtgcatgg ccatttcttt gaatgcatct gtttcattgt tcatggcacc cagaagcaag 90780 tgtttggaaa gtctcacgtg ctgaaacatg gccacaagcc aggtctccca gcccagcctc 90840 ttgtctctct ccccatcctc tttgtttagt aggggtaata tggagcctgt agggaaggca 90900 tctatcagct aggatgcaga tcgtccccac agacccaaaa gacgtctatt tactttcttg 90960 agagattgag agttctggcc agatctggga aacacttttc aacattctca cgctgacctt 91020 tgaggtatgt atagtgaact cagaggagct gcctccccta ctgtgaatta agccaattat 91080 tgtggaattg aagtctgaac tcttccattc aagctcacca gaaaaggaag tgatgtcagg 91140 catttactga gagctttctg tgtcccaggc attgtaccag gtaggttatc ttaggcacct 91200 gatcttaaaa tggggaaatg ggctttaaaa tatggcgatt tcctgctctg gtgctcatgt 91260 gaagaactgc atgttcctat gctgatagca ctgcaggtga ccctgtcaac ccggggtttg 91320 tgaccatatg tccctctggt aagtgggtga agcccacaga cctcttccca aaataatgtt 91380 tttatataca taaagcgaaa taaataggat tataaatgaa atcaattgtt tcaaaatagt 91440 attatcaaaa tatattagca tactaagtaa caacatctag tgacagggct agtagctacc 91500 acaattttga agtagataca agtgtagaag ctggaaatca actggtgcat tcttctcaat 91560 cctaggaaac cactggaccc cagattctct ccagaatcca tttcttcact ttagttaaaa 91620 attagaattg acaaccgggt gcagtggctc atgcctgtaa tcccagcact ttgggaggcc 91680 aaggtgggtg gatcacttga gctcaggagt tcgaggccag cctgggcaac atggcaaaac 91740 cccatctcta ctaaaaatac aaaaattagc tgggcatggt gtcaggtgcc tataattcca 91800 gctacttgtg aggccaaggc accagaattg cttgaacttc ccgggaaggg acactgtctt 91860 aaaaaaaaaa aaaaaaaaaa gaattgacta gatagaaagg aaaagccaaa ttaacaaagg 91920 tataagcaag atataagttt gttttatatc acatgaaagc agtctggata gaggcagtcc 91980 ggggctggtg tagtagctca gcggtcatca tcaaaggccc caattccttt tttcttactg 92040 ctcctccatt cgtagcatgt tccaactgta gggttggaat tctaggcagc acaaagtggg 92100 aaggattcat aaggatatgt ctgtactccc agaaagaatc ttcctggaag tcccacacaa 92160 atccacttga atctcattgg ccagaatttc aagacaaaga aagtcttgga aatgtagagt 92220 agctgtttat tcccggcagc agggagaata caaatcagtg tagagtggac acagggaggc 92280 aactggccat ctctgctgca ccatttcatt cttactctgg aaaatctttt aggaatgttc 92340 ctgcatttga taatctaatg tttaagtgtg gcccttccat ccatttttgg atgccagaaa 92400 cagaaagcaa ctctggctaa ctcaacagga atttcttgaa aggctattgg atagctcaag 92460 gaatggtcag gaaggctgga gaaacaggct cagaaaacag cagaagacaa aggaatcctt 92520 ccttagatgg cttacatggc ctttcagcaa actcaaaatt tcagcactca gacccaaaga 92580 aaacacttac agtttaacaa aaattggccc aaattacaca acttcagttt tagtgcccca 92640 aggggctttt aatattgtta gattttcttc caaagtcatg gggagtcgct gtctttgcaa 92700 gatgcaatcc ttaggggtgc cgcctgctgt tagtaaatca taattaccca agcatgacgt 92760 tgtggacaag tcaccccagt gccctcattc agataagtgc tgagtacaca ctaatgtgtg 92820 ttaggaggag aatctgggcc catttgaagg taggcatagt ttgggctgct gtggcctttc 92880 tatttggttt cagagaatgt agatacaggg gtctcgagac cctcaagcca gtgtgactcc 92940 tctttagcat ttcagagtgg attagacttc cctggtactc agatcacagt tggctagatc 93000 ctgggtcttc cccaaaccaa atgtgaatct gagccttacc agcagactag atccccctca 93060 aatcctggag accttgacat acagaagaga caaaggcacc catacctatg gtaaataaaa 93120 gtagtaacat tggttttact tattgtagat atagatgcct actccatgag ctgttgatgt 93180 tggggtttgt cctgaaaaca aattgtgaat aaatcgtgaa tacattttat agtattaaaa 93240 aattagtata aaaaattaat ggcaatatca aatgctggaa aggatacaga gaaacagcat 93300 ctctcatgca ttgcttgtgg aaatgaaaga tagttatgga cactctggaa agtggtttga 93360 cagtttcttt aaaaaccaaa catacaccta acatatcact cctgggtgtt tatcccagag 93420 aaataagaac ttgtatccac acaaaagcgt atgcatggtt atccatacaa ctttatttgt 93480 aacacccaaa agctgaaaat aaccaaaatg tcctgcaata ctataaaatg ctactcagca 93540 ataggaaaga agtgattgct ataacagcat gtatggacct caacagcatt ttgcagagtg 93600 aaaaggccaa tctcaaaagg catttatgta acattctaga aatgacaaat atagaggtgg 93660 gagaacatgt taattattgg cagaggtttg ggatgattgg ggggtggggg gtgtatgact 93720 ataaaggagt tgcatctggg agaactttgc gttgacgtca tagttgagtg tcttggtttt 93780 caatatggtt atgtgaatct acacatgtga taaaataaaa cagaactata tacacacatt 93840 gcactaacgt cagttccatg ctttcgatgt tgtctatagt tatgcaagat taaccattga 93900 gggaaactgg atgaagtgta catgggatct ctctgtacta tctttgcaat cttctgtgaa 93960 tctataatta attcaaaata aaaagtagaa aacaaatgag taataccttt caagagggca 94020 gtcttacata tattatctat ttattctaac aattttgggg ggaaaaacaa cagtaactat 94080 tactaccctt ttttgaatag agatcctgtt gcatagaaat ttggtcattc cccagattga 94140 cacagataat ggaagattag acatgagaag caatcatttg atccttattt cttcaaaatg 94200 ttctctgtac atgaagaagc taagaaactt ctccagagag caacacagag ttggaatggt 94260 gtctggggca tttcaactgc ctctacagtg tgcatgtgtg gcccagatgc cagctacccc 94320 ttgtctgtcc aggcgatgct ctcacagccc cttgccaagc attcagagac ctaagnnnnn 94380 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngatat tacacacaca 94440 tatacataca tatcaaaaga catatacata tataaataac catagaagat ataacataat 94500 agcatacaag aaaattatta tagttatata tcaaaaatta tataaagaat agaagagagg 94560 aaagaatagc atagaagtac agagaagtag agaagaacaa gataagagcc aacattatat 94620 tataaaaaat aatattataa aaaattaact agaagcatag atgagcacac acatagtagc 94680 acatagatat ataggagaag atagaatata ggagaagaat agcagagcat aggagtatga 94740 ggattacaat gagtcatggt caggccaaat gcactacaag aatgggtgac agagcaagac 94800 catgtatctt tatatactat actatatata tatatatata tatatatata tatatatata 94860 tatatatata tatatatata tatgtatatg tatataaaga tttcataaga gactgaagaa 94920 gttttttaca tcacatgctt tataacccac atttgataaa tcctgatttg atacttgtgt 94980 ttccctcaaa gacaacataa cttctctctt ctcctcccaa cacagcatgc aacttctcta 95040 gctcctctct tcctccagga atcaactcca ctgtcacccc caacccctta ttactcagag 95100 aaatcaagct cttccagctt ctggctccaa acttgcatac ctgtcttaat ctgctcaggc 95160 tgccataaca agataccaca gactgggtgg cttaaacagc agaaatttat ttctcacagt 95220 tctggatgct tgaagttcaa gattaacatg tcggctgagt tggcttctgg ccaccttctt 95280 gctgtgtctg tacgtggtgg ggatggggaa gtggtggggg gatgggcatc tctctcttct 95340 gtttttataa ggccacagtc ctgtcagact agggtcccac ccttatgagc tcatttaacc 95400 ttaattacct cccaaagacc ctatcacttt caggcatatt gggggttaga gcttccacaa 95460 tgaatctgag agacataatt cagtccataa aaccacccat tatgatctta gcaaacaaag 95520 ggccctctag ctactagaag ggctccaccc tttgaaggca ctgggatttt ttgggggttt 95580 ttttcctgct gttttattgt tagctaccta ctgcaaacct aacttgaaac acggatatct 95640 tttctctcta aaataaggtc ttgtgctatc attatgattc cataagaacg ccccatctca 95700 ttttcctgca catttttata ttctgcacta gacaaaatat gtatgtgtgt gtatgtgtgt 95760 atttctttga aaataactgt tctgaaaata cccttgctgg gctgaattta tcattaacct 95820 aattagatcc tttatcaaag gcttacaatt aacaccatta gcaagtctta gtaactcacc 95880 gctgtgtttc taaattaact ttcacctgta gggacataaa aatcgccatc attttctaaa 95940 atattccttg tttgagtgtt gtaattgttc cccagatttc catgaataat attaagtgac 96000 ttacaggaca agagatactt acaattttgt ggaaagggag agaatgcttc cctcatatct 96060 tagggtacaa gttgaataac ctcaaaatcc tttttcttcc atatctagag tggggtcccc 96120 agaaggagta ctcccttaat ttgcccaggt tgttaaggta aaaaattgta gagctaccca 96180 tctgttcttc acaagcacag tattctttca tttgcaagga ctaagccaaa ctttattttt 96240 ttacataacc taccatcaga ggcattgatg ttaaattacc taccagttcc ctattctctt 96300 atgggctgat tttgtgtttc tattttattc agctgtctct gtcaaaatca agtttatatt 96360 tgccctgagt tgctttgaga aggtctcaag agaatccaag gggacactgg gtatggcatc 96420 ttagccagtg tggccattca gcaccactgg gccacactga tgtttcctat gtgactaatt 96480 tcctgagatg tacaatcaac tgcctagagt agagcattag aaaaatagat tgctaaatac 96540 gacagagtgt gatgttgaaa gcttgacagg gaatctaatt atgcagtgaa gtaattccta 96600 gggatccttc tcaatgatgg aaggggcttc ttctttggga ggtttctgat cttttatgta 96660 acactgaggc atgcttccac ttgaggataa attaaattgc atgatccact tcttcctcat 96720 tcccatgctc agccctcaag gagctcttca gctgtgctgt ctgtcacacc atggaatgtc 96780 taagacaaaa gggagttact tggggcttgg acaggatact tgcaaagagg ttggaaaaca 96840 atgagcctga gaactgggtg gcaggagatg gcagtggtca gccttgagat ctggtgaaat 96900 caaaggaggc agactgtgat gaaaggaaac agtatgggtt agaacagagt gaagtggtta 96960 ctgtagatct taatcaaaca aaaaaaattg ctcttgtgta atctagtggt agccgaagag 97020 atgagccatt taagccattt atgtattccg tgcccagtct ttccaatgat tagtagcaca 97080 tcttaatgag ataaccagca tgggttaggc aactggttga gggccatgga gcaaatatat 97140 tttctctgtc taaagtatcc agcaaagaag tggattcaat ggctttgcct ccattacagc 97200 taaacaaact gggattgagc cttcatttaa attacttgat gagagtataa cactacattt 97260 tgggtttggc aaatgctcat tgggcatccg tctgtgcctg gccccatgct ggaagccaga 97320 ggtgcttcta ggctaaggtt ggactgcgaa gaaatctctg gtctggggta acagcaaaga 97380 gtcctgaaga tgaaaatcct tgcttctcat cttgcctgtt tcagcttatg agttcaacga 97440 accgcttcct caaatactgt aatggatctg ggagttcagc cttgctgggg ccactttgaa 97500 ttccactctg ttctttaacc tacactggtc ttcctagtta gatggagggt atacattagc 97560 ctgagcagtt aggtggatgg ctggggagca gtggggcaga aggaaaatga gttgggaaga 97620 gtcccagggg tcttggcttg acaatttaag actgagatgg gcaacttaaa tatcctagga 97680 accaagcagg tggcatgaat aaatgaagct ggcagaaaag ggaacagaag caaatgagaa 97740 agcccacacc ctctatagag gattagcagc tactgagctg cagcagattc cagccataca 97800 gatattaaag cccagtttgg ccagatctga tttccaaaaa aaaggcaaaa ttctggatat 97860 ttatatgaaa tctctcccat ttttaatggt ctaattctta tgtattctta tagagataca 97920 atttacacat gtggtaagta gaataatggt ctcccaaaga tgtccatttg gtaagtacat 97980 gtttagcttg atgagaaact actaaactat ttctaaagtc actttcccat aatcctccaa 98040 tgtttaaatg ttggcagcta attcaggaag tttcaaagca aggtataggc ctaacagaac 98100 acacgtgcag gtcagatgtc agatgtgtcc caatgttaag gagagctgca ctggccagga 98160 gaggaagaca cgggtgatgg gcctgtgctg atggagaaag atgtgtctct ctgggagcct 98220 gaatagggga agattctcag aattaggaga aaggcaggtg ggatgcccat gatcactggc 98280 tgtgtctggc aagcagttgt gtccttaaac caaaggaagt taagcgggcc ccacaggaaa 98340 agagttccag ggaccagaga gagaaagagc ttggtttcag acagagattg gaagtgatga 98400 cagaaagggc ctgctcaagg aaaggaagga cctcaggggc cttccgggag ggatggaaag 98460 acaccaaaca tctgattggc aggcgatgac accttacatg agaagtaccc ttggtgattt 98520 ataaagattt tcagaaacat tatctcatga tgcagataaa agccaaactg catgacggcc 98580 tgtactgcac aggggctggc caggcagaag accccatagt gaacaagcag ctgggccagc 98640 tccttgcagg agcaccttcc ctctgccata tgtggtctgt gctcgaggat actccaagcc 98700 agggtatggt ttctgtcttg tagtggaccg agggtggggt tccacaaaca tagagaaaga 98760 aaagctgtag aaggggcatt tcccccctgt ctggctcaaa gggggttctg gaggcaccag 98820 taacccagtg ccttcctagg gcatcctacc accctctcct tgtgcctgat acacatctct 98880 atcttacaga attgtcttta taattgcaat aaaataataa atatgtttct tcagaactct 98940 aggcaatttc tggactcttt gaggccagag ctgaagtctt agttatcttt ataacctccg 99000 tggcactgaa cacaatgcat gagccagata agcccacggt gagtgttttg ctgacttgag 99060 tcacagagcc ctgtaataaa caggctactc aggggtgtca gtcccctcca cacatctggt 99120 tgagacagtt ccatctagtt ctgatcagtt ttattaaata agcttgtgct gtggggctgt 99180 tttttccttt gtgaactggc atttccagaa tgggttacca cgctggtggt ggccctagct 99240 gatgggcttg aaggggaggc attcatcttg tcccatcgcg gctcacttag ctgagacctc 99300 tgcaggagct tacacgcggg ccagcagaat cgcaaatagt tctcccacca cccgaggttt 99360 tatcattgct aagctactgc aagattattg taattgaaat ggttctcagg cttctggggt 99420 tctttctctc tatctctctc tctctctctc tttctctctc tttttttcta gcctgtgact 99480 cacatttttt tctaaggcat tgtatcagtg gcctttgcag gctgctcaag ggatccagac 99540 ttttgcattt attgaacatg tcaggcattc attaagtact gcaccatcag ggagaatttc 99600 cctgctatta agcgtcgact ccagccaaat cagattcacc tgggcgtttc tcaaagaaaa 99660 gcctgtcagc atgataatgg tttgaacagg gctgagctgt atggttgaag tgcgacaggg 99720 cagaagacag gctgtggtga ccccagagtt ggtctgtaac tcacagagga atcacacagt 99780 ggagaggctt cagaaaatca gaaatctgac tgtcacaggt agcggggcaa gctgcctgat 99840 gatgcacttc aggaaggctc agcaggaggg tggggaggat ggaaaaaccc gtaacggagc 99900 agctagaaaa ttaaggcaag gaaaccgtaa gtctgccctc cagactgcca cggttttatg 99960 tttgagactg aggtcactcc cccagggaaa acaagaggaa ctgagaggtg agagtagggg 100020 agaaacaccc ctcactcacc ccttgaataa aaactttggt gtcagtagca tcctgtacta 100080 atgtttattg agcatttatt atgtatcata cttcattcca agaagtgaag gtatattctt 100140 taatttttac aactcttcta tgaagtacgc attaatatta tctatgtttt acaggtgagg 100200 aaactgaggc cgggaagtta aatggcttgc cctaggtcac ccagctagta aggtggagga 100260 gctaggattc agggccatgc catgcctcca gataccacaa ccttagtggc tttactatgt 100320 ggttttccat tccattgggc tgacacccaa gaagccacag gtactcactg tggcccatgc 100380 ccagctttct gtcaaacatt cctcaagttc aagggcaatg ccttttcccc tcctcccctg 100440 cccacaaaac gtgtcctcca aatggcccag ctgagaaagc agggaaattt ctagaaatat 100500 ttgcataaac tttgggtgaa agtggaaacc ccaccaacac ccatcctcca tcctccccac 100560 tctgctgcct atgtctgcca ggctgaggat cctcaccctg gtgcgggacg gccatgtggc 100620 cattcagagt tcttcctacc cccatccaac tttgcagtgc tgttggttgc ttcagagctt 100680 gaggatctct ggacagatag gatttgctct caggtttcac attgcttcct tccttcaggc 100740 tggacaatta gaaatgggta ggagggtagt aaaatggatg cgatgaacta ttccttccag 100800 gcaggcagcc caagctgttg gccctcttct ttccatttgg acttggagca agtccttcag 100860 tggggttagt tactaggagc catgtcagct gaccctcact cctttaagac tttcccacct 100920 tcatgctcac ctgtacataa gttctttgtc actttctgat gaagtctgag cctccaaatt 100980 ccaaagctaa agtgtagtta gcccctacca tttcctgttt agacagggca agatggcacc 101040 cagcagagga gagagaggac atatatgtgg acaatgccct tatcccctgg ggggcttgca 101100 taacaagggc gaaagccctc cctctttctc aaagtcatgc tgaggctgga cctcaataac 101160 tgagaaagaa gagtgacggg gagggtgaag aggttgggga gaggaagctt gaactaaaat 101220 tctgaagtac atgagatatt gtatgctggt gcagtcattc tggagaacaa tcaatgatgc 101280 ttgtcaaaat atgtaaatat atacacacca atcaagcata cccccatctc tggtatatgc 101340 ccggagaaat tcacatgtaa gtcatgagta aggatgttca caacatgttg tttgtgaaag 101400 gagagagtta gagaatctgt ctgtccatca ctacgggaat gggtatgcac aaggcagggc 101460 agtactttgc atcaattaga aacaatcatg gtaatttgat acatcataaa agcagctgag 101520 tgtaaaggaa gacaaatgtg aggactatat cgtgttgtca ttatgtaaat gaaaagacac 101580 aaatttatct tgcaaggatt tactcacatt caaatccaca attaaaacac attggctatg 101640 atggcagtca tggaagagaa tgagagtgaa gaatgttcat aaaagggata attaacaatg 101700 caattatttg cacaatgctg tatcaacagc atcaatgatg tggtaccatg atatggtcat 101760 ggggagggta atgaattcag ttctctgcac ctaaggttga aaagaaaata aaatgtcaag 101820 agtttcattg gagttcattt taattattca tgtaggcaat attgtctaag cataagattt 101880 atgaagaaca aataacatag catggctggg cgtggtgatc caagcacttt gggaggccaa 101940 ggcgggtgga tcacttgagt ccaggagttc gagaccagcc tggccaacat ggtgaaacca 102000 tctctactaa aattacaaaa attatctggg cgtgatggcg ggcacctgta atcccagcta 102060 cttgggaggc tgagacagga gaatcgcttg aacccaggaa gcagaggttg cagtgagcca 102120 agattgcacc actgcactgc agcctgggtg acagagcaag actctgtctc agaagaaaaa 102180 aaaagaaaag aaaagaaaga aaggcaagaa agtaagaaaa gaaaaggaat aacatagtcc 102240 tggactatga atcagaggct atattttctt acttacacct ttgcaccctt gggttctcga 102300 agcctcagtc ctgacacaca ttaactagaa aatgtttatt taattctaac ataacatcct 102360 gagttcctgt gtaagtaatt tgcaaaagcc aaagaatgga ttaggaactc tctgaatgaa 102420 atttctctgc ccagtgcagg aacctgcagg gttaattggt gcctgtgaat acagtatagc 102480 cacagtcatt ttccaaggtt acaaaacacc ccctcgtcag ttcagttgtt tttctttggc 102540 cttcaaagac ctaagtgtta cagttaccat tctgtgcaat agatcactaa agtttattct 102600 tcatgtccta ctgaaatttt ttacctttta ataagtatgt gaaatgatgg atttattagt 102660 tagtttgatt taatcatttc acaatgtaaa catatgttac aatatcacac tgtaccccat 102720 aaatatatta atatatacag ttattatttg tcaatttatg aaaagatcca ggagttaatg 102780 tactgaggct tgctgcagct catggctgat tttatttatt tatttttttt tattatactt 102840 taagttttag ggtacatgtg cacattgtgc aggttagtta catacgtata catgtgccat 102900 gctggtgcgc cgcacccact aactcgtcat ctagcattag gtatatctcc caatgctatc 102960 cctccccgct ccccccactc caccacagtc cccagagtgt gatattcccc ttcctgtgtc 103020 catgtgatct cattgttcaa ttcccaccta tgagtgagaa tatgcggtgt ttggtgtttt 103080 gttcttgtga tagtttactg agaacgatga tttccaattt catccatgtc cctacaaagg 103140 acatgaactc atcatttttt atggctgcat agtattccat ggtgtatatg tgccacattt 103200 tcttaatcca gtctatcact gttggacatt tgggttggtt ccaagtcttt gctattgtga 103260 ataatgccgc aataaacata cgtgtgcatg tgtctttata gcagcatgat ttataatcct 103320 ttgggtatat accaagtaat gggatggctg agtcaaatgg tatttccagt tctagatccc 103380 tgaggaatcg tcacaccgac ttccacaatg gttgaactag tttacagtcc caccaacagt 103440 gtaaaagtgt tcctatttct ccacatcctc tccagcacct gttgtttcct cactttttaa 103500 tgattgccat tctaactggt gtgagttggt atctcattgt ggttttgatt tgcatttctc 103560 tgatggccag tgatggtgag cattttttca tgtgtttttt ggctgcataa atgtcttctt 103620 ttgagaagtg tctgttcatg tcctttgccc actttttgat ggggttgttt gtttttttct 103680 tgtaaatttg ttggagttca ttgtagattc tggatattag ccctttgtca gatgagtagg 103740 ttgcgaaaat tttctctcat tttgtaggtt gcctgttcac tctgatggta gtttcttttg 103800 ctgtgcagaa gctctttagt ttaattagat cccatttgtc aattttggct tttgttgcca 103860 ttgcttttgg tgttttagac atgaagtcct tgcccatgcc tatgtcctga atggtaatgc 103920 ctaggttttc ttctagggtt tttatggttt taggtctaat gtgtaagtgt ttaatccatc 103980 ttgaattggt ttttgtataa ggtgtaagga agggatccag tttcagcttt ctacatatgg 104040 ctagccagtt ttcccagcac catttattaa atagggaatc ctttccccat tgcttgtttt 104100 tctcaggttt gtcaaagatc agatagttgt agatatgcgg cattatttct gagggctctg 104160 ttctgttcca ttgatctata tctctgtttt ggtaccagta ccatgctgtt ttggttactg 104220 tagccttgta gcatagtttg aagtcaggta ttgtgatgcc tccagctttg ttcttttgga 104280 ttaggattga cttggcgatg cgggctcttt tttggttcca tatgaacttt caagtagttt 104340 tttccaattc tgtgaagaaa gtcattggta gcttgatggg gatggcattg aatctgtaaa 104400 ttaccttggg cagtatggcc tttttcacga tattgattct tcctacccat gagcatggga 104460 atgttcttcc atttgtttgt atcctctttt attttgttga gcagtggttt gtagttctcc 104520 cttgaagagg tccttcacat ccccttgtaa gttggattcc taggtatttt attctctttg 104580 aagcaattgt gaatgggagt tcactcatga tttggctctc tgtttgtctg ttgttggtgt 104640 ataagaatgc ttgtgatttt tgtacattga ttttgtatcc tgagactttg ctgaagttgc 104700 ttatcagctt aaggagattt tgggctgaga caatggggtt ttctagatat acaatcacgt 104760 cgtctgcaaa cagggacaat ttgacttcct cttttcctaa ttgaataccc tttatttcct 104820 tctcctgcct aattgccctg gccagaactt ccaacactat gttgaatagg agtggtgaga 104880 gagggcatcc ctgtcttgtg ccagttttca aagggaatgc ttccagtttt tgcccattca 104940 gtatgatatt tgctgtgggt ttgtcataga tagctcttat tattttgaaa tatgtcccat 105000 caatacctaa tttattgaga gtttttagca tgaagggttg ttgaattttg tcaaaggcct 105060 cttctgcatc tgttgagata atcatgtggt ttttgtcttt ggctctgttt atatgctgga 105120 ttacatttat tgatttgcgt atattgaacc agccttgcat cccagggatg aagcccactt 105180 gatcatggtg gataagcttt ttgatgtgct gttgcattcg ttttgccagt attttattga 105240 ggatttttgc atcaatgttc atcaaggata ttggtctaaa attctctttt ttggttgtgt 105300 ctctgagctc atggctgatt ttatttcttc tagtcttggg aagtttttta ttgaaatatt 105360 ttttatgtca taacaatgaa attgcattaa ttcacaacat ggagggacaa gctcttttag 105420 ggagacttct atgtatataa ttattttaaa caaatgttat ttgcttaata tgtcatggat 105480 ttttataagc cataatgtat ttgagcatat cagtttatag tatatgatat ggtgtgttat 105540 tcaataaata tttaaatgtg aaggaactag taattagaaa cacctatgac atgttcctag 105600 aactgcctca cacagcaatt taatattttc tctttttgct gcaacaaatt aagtgtatca 105660 gttcacactg agcaataggg tcactattat atcagtctgt gcatgtttct gaaaaaaatt 105720 attactcaaa ttctaaagca cgatttataa ataacatgtc cattttatca aactcagtgt 105780 aacatattcc aggtgttctt tcaatgatct tatacaaaag atctggcaag ttttcttaga 105840 ggaaagtttt ccggagtttt tactcagtaa gaatcaaatg ctttatgtct ttggtactgt 105900 gattccagaa ttggaggaat ccaaagggat gaagttttat tctgatatat tattccactt 105960 catcataaca acctgatttc agccttctgt ttctcctggt tgcccacaga atcactgcca 106020 ccgtccttaa aaacaagcat tctggccctt tgcaagagca ttataaatca tggtttcatt 106080 ttagggaaaa ggttattgat ttgctcattt tcccctgcct ccctctggtg cagacttcag 106140 tgaaaaactg aagcttcctt tatgaagtat atcatagcca atcaaaagtg tggcctgtgc 106200 ttacttgatt tataatgaaa ggtgacacga gtgagggatt tcacactgct catcctacag 106260 tgactgtaat caagagataa aagctagagc aactacaaat gctggccaga cttcatcaga 106320 attatttact gcgtttgcta agacagggac attcgatcag tttagatgga agaactaaca 106380 cagttaaagg cgatgcattt gtgtttcatt ttgcttttaa agccagctaa cattccactt 106440 ttcctatcgt aattaacttg gccttttaag attgtaccca attgtgattt gctttaagtg 106500 attttttttt tcacaaaagg atcagaaaaa agacaaaggg agaattatcc atggagaaaa 106560 gggcaaaaaa aaaaaaaaaa ggaagtaaag tagtgatgag acatttaacg atatttttga 106620 aaggcctaaa gtcaaggaat aagtgaatat cattagtaaa atgcagaaat gccatgaaat 106680 gtcttggcag caaggtaaat gaggccccgt tagattttct ttctggttat gttatggatt 106740 tttctaaggc atgatataca ttggttggta aagggaaagc cagcaaagct ttctttatca 106800 gtggtgaaag aaaacatgtc tgaagctggg acatttgcag tatgatttta agtatattta 106860 tagagagatt gaaattacaa ataaaaataa taaaaatcat atcaatgtaa acatttacat 106920 aggaagagta ctgcagtgtg attaaatgtt tgtagtgtgg ccgaaactca gaacacagaa 106980 tgctgtcact gaggtgtatt gtgaatgctt atatggaaaa tcccaggcta tcgatttcac 107040 atggcctttg cttagtgcaa taaaaaaagg gattaagatt ttttttcaag cataataaaa 107100 catctgtgga ctcattttca gatctagaga aaaacatcat tattttaaga atccatattt 107160 tttttccttt ccatttagct cacacttact tagtacattt cattaattgc gtctcctaca 107220 gctgagcatg actctttttg catctaaatt gattaaaata gagaacagtt ggtgatatga 107280 gttgtcttcc ccccaccggt tctatatgaa attgaataat ttatgtattg tagaagacag 107340 agtgatctta atatagaaat tgtatttgca gagctcagtg gaggttccca tgttttctta 107400 caaagaaact gatcttttgc cttagctgag ccatttcttc tctaagataa aatgaggtaa 107460 cttttttgtt ggcacatgct ccacacacca tgtcagcagc atacccaaag acttctaaga 107520 caactcttta gttatcaaac aaaactggct ctgtggcaca atggatagca cgtaaaagtt 107580 agttcatagc ttcagaatac attttacctt ttatagtcaa tattgaaaat caaagtaaca 107640 tgtccatagt cctaaaaatc aaatacacta ggcctgggca tggtggctca cacctgtaat 107700 cccagcactc tgggaggaag gcaggtggat cacttcagga gttgaaggcc agcctggtca 107760 gcatggtgaa accccgtctc tactaaaaat acagaattat ccaggcatgg tggcaggtgc 107820 ctataatccc agctactcgg gaagcttagg tgggagcatc acttgaaccc agggggcaga 107880 ggttgcaatg atccaagatc gcatcactgc actccagcct gggcaacaga atgagaccct 107940 gtatcaaaaa aataaaataa aataaagaga gagagagaga aatagagagg agggagatgc 108000 caggttcttt ttaacaacca gctcttatcg gaacaaataa agtgagaact cactcatttt 108060 ctaccactgc agggagagca ttaatctatt catgatagat ctacctccat gacccaaata 108120 tttcccatta ggccccacct ccgacactgg gggtcaaatt tcaacatgaa accaattgat 108180 atggtttgac tctgtgtccc cacccatatc ttgcctcaaa ttgtaatccc cataatcccc 108240 acatgtcaaa ggcgggaaca ggtggaggta attgcatcct gggggtagtt tcccccatgc 108300 tgttctcatg acagtgagtg agttctcatg agatctgatg gttttataag catcgggcat 108360 ttgccctgct tgcactcact ccgtcctgat gccctgtgaa gaaggtgcct gcttctcctt 108420 tgccttcaat catgattgta agcttcctga ggctttccca gcaatgtgga actgtgagtc 108480 agtcaattaa acctctttcc tttataaatt ccccagtcgt gggtatttct tcatagcagt 108540 gtgagaatgg aaaaacacac caatctatac caatcagtaa tcagaattta cattattctg 108600 acttctactg aaccaagtag tatattgagt atgattactt tctcatacaa cttttgtctt 108660 tcctggagtt aacaattata ttgtgttttt atttgcctgg ttttctatgt gtgtttcaca 108720 aattcttccc atatctatag tctcataatg taggttttca acataatgaa atgtattggg 108780 tgaactatct taccaccatt tttttactga agacatttct ccgagagttc ttggtccttc 108840 cgtttcttag gtctgctgca tatctgtcat cctggaactt ctctccactg ctcccctgag 108900 ttgatctatt tctggaaccc ctggactttc tgacttatac cttctttttg gtgaagtata 108960 atctttggca atttctatag aaaagttaaa cagaagtagg ttgctgttgt tattgtgttt 109020 tggtccttgc ataactgaca gtgtctttat cataccacat actatattga tagtatggct 109080 ggacatagaa ttctaggttg gaaatcattc tacctcagaa tgtttgaaga tattgcccac 109140 tgtcatctag catccagcat tgttgataaa tctgatgcct ttatgattcc cttggcttta 109200 tatatgacct gtattttctg tgagttctta tctactcttt atctgtggta tcatgatcat 109260 aatattcaaa tgtgtctgtt actttctctg cactttgctg agatttattg agccatttta 109320 atttgaaggc ttatggccct agcccagagg actaacacac acacacacac acacacatac 109380 atacatacac acatactcac acacacacac acacacacac acatatatat acctcaccac 109440 catttttttt gttgttgttc tcactttctg gaatgtccgt ctaatgggtg ttgaactacc 109500 tggatccatt ttctaatatt ttgatctcct cattcctatt atccatccct ttgtcttttt 109560 attctccttt ctaggagctt ctttatatct tccaaccatt ctattgagtt tttatttctg 109620 ttattactat atttttattt ctaaggaggg gtgtgtgtgt atgtgtgtgt gtgtgtatgt 109680 gtgtatgttc tttgcccttt ttaatccaac ctttcattgt ttcatggctg tatcatgttc 109740 tcctacatct ctaagggatt gactatgagg ttttgggatg ggagtgatcc gcgttttgtt 109800 ccctgtagtg tctgtccttc tgagtttctt tttttctgtt gttttggtct ctctttcatg 109860 ttggacactc tcttagtacc tgaaagtctt cagttatcta ttaatattta agagcgaagc 109920 acggaaaatc tgactgaaaa ctgaataagg ggagcttgtc aactagggtt gagggtgagg 109980 gtgtgtcaca gtcaggcgtt ctagcagcag gcagcctttt aatccaaagg tactctaatt 110040 gtttgaattt gtctcttttt tagaagacta ttttctgtga aaacgaacaa ttcattttcc 110100 atattggagg agtaagcctg actcctgggg ttctgagaag gatgtggagg ggaatgccac 110160 actattcaca ctggtgaaaa ctttgactta aatcctctac ttttagtctc acacttaact 110220 cattttcttt ctgattgatt tacccaagtc cagactattg caacatgagt gaagggtagt 110280 cctctgattg cttggctggg gagaagtctg aagttctgcc cactgcattc acaggttttc 110340 gatgaatttc attattttca gcctgtgtct cactcctatc ctcctaagta tctggtgcct 110400 tcaacatctg acccttttgg gatcctaggg aataaatagt cttccccccc acttctgtat 110460 gtcctgacac cacagtttcc ttggctccac taagccagtt attcttcctc tgtctgcttg 110520 ctgtcttcca aaatcttgtc gagatctcat ccctcattat ctcctctcca gttttctttg 110580 ttcttgttct ttattccttt acccttacat agtgatgttt gaaatgacag aatgatattt 110640 agtctgccat gtccattgag cagtcaagaa taagttctcc atgtggaatc aaaggagata 110700 gcagagcaaa taaatgctgt acttgtgctc tattgctgca taacaaatca ctccaaaact 110760 ccgtggctta aaacaataac catttattat ttcatggttt ctctgggtca ggaatttaga 110820 cagtgtgcag aggagatggc ttgtctctgc cgcatgatac ctggggtctt tgctggcagt 110880 attaaaggct gggggcctag tattatgtga agacttatct aaggctgaag gatccactta 110940 caagggggct tactcacttg gctaccaagt tggtcctggc aagttggttc atctccacat 111000 gagcctctcc ccaaatgggc ctctccaccc tgcttgagtg tcctcactat taaaggttag 111060 caccccctaa ccccaagcta gtgatcaaag agtaactaag gcaaaatctg caatgccttt 111120 cctgacctag cctgggaagt cacacactgt cacttctaca gcattctgtt gccacccatg 111180 ctagccacaa ttcattaatg tatgaatccc aggtgatgtg gatcgctagg ggcatcttgg 111240 aggctagctg tcacaaatgc ccaactccca ggagggctat ctgcttccct tctttgcaac 111300 atgattttgt gttggaaaga agtcagaaaa aatattttcc tccctgtctt aagctaagat 111360 gttggtgttt ttgttccttt tcctttctta tgctttttat tctgcaccat tcatcaaata 111420 ttgtgccctt tttctttgaa atcttgaaag atgaagaatg tgaaagcaaa agagaagaat 111480 ctaatgccta ctatttgttt tggtggcacc cctacagcat aaaattgtgg cacgtgtgag 111540 ggagaacata gtgttggttc agtgccagcc aagggagact gcattcccac acccctaccc 111600 caaatgtggc aagtaataat tacacagcag ttgtgtgata acttctgctt aaagcagatg 111660 tattttgaca catcacctgt cccctgatat cagtgtagct gtgtaaacag tagtgtgtac 111720 attaccaaca ttttacccca agcatcccct tagggtctcc gattttctgc ctcagggctc 111780 tctccaaaga cctctcatcc ccacacagac tcagcctcga agtgcagggg aagtaactaa 111840 ctaggaatca accctcaact aaaagacaac agaaggcagt ggataattgt cctctggagg 111900 aaccatctgt gacacattct acatggttcc ttagagggtc cccaagtggg attgagtccc 111960 tcttgttcct agcagtaagc agttctgtta tacactcttt atatgctttt cctcctttcc 112020 tctctcagtc tctctgatcc ttccacctgt gctctgggtt cacctctcaa gtaaaccacc 112080 tgcacacaag tccctgtctc aggctccatt tggggaaacc aaactaagac accacctcaa 112140 aacacagaat ttcacgaggc tccaaatgcc tcagaaaagt aatgtaaata taaaatgacc 112200 aattttttga agtatattaa tcctagttat gacactacag gcagatgcca gataaatatt 112260 tatcattgat tagatccact attattaaaa atacaaactc ccagatccca ccccatttcc 112320 actttttttt gagatggagt ctcactctgt cacccaggtt gcagtggcag tggcgtgatc 112380 tcagctcact gcaacctcca cctcccaggt tcaagcgatt ctcctgcctc agcctcccga 112440 gtagctggga ctacaagcgc acgccaccac acccagctaa tttttgtatt tttagtagag 112500 acggggtttc accatgttgt ccaggatggt ctcaatctct tttttttttt ttttttccca 112560 agccaaactg tatccagctt tattaaagat actttccata aacaatcatg gtatttcagg 112620 caggacatgg gcagacaatc gttaacagta tacaacaact ttcaaactcc cttcttcaat 112680 ggactaccaa aaatcagaaa gccactataa aacccaatga agtcttcatc tgatgctctg 112740 aacagggaaa gtttagagtg agggttgaca tttcacattt agcatgttgt ttaacaactt 112800 ttcacaagcc gaccctgact ttcaggaagt taaatgaaaa tggcagaatt tatctgaaga 112860 tccataatct agaaacagaa ccactgctct tttgacaggt gccatctcag tggcatcact 112920 ggaaagtcca gattgcctaa cacactggta accaatgact aggggtcagg tcccaacaga 112980 tgtctgggct taagggagtt aagtctatgc tgaaagaggg aaagggagac gaggacataa 113040 aaacaaattt gtttttctat accacaaggc ttttgtgcca aggtggccat gtgtgtcaaa 113100 gtcagggaat ccctcctcct ggaagccaag aggaagtctc tcaaaactag aagggaaagg 113160 tgttttctcc acatcaatcc agctttggag acattctatt agcgacatat gccccttccc 113220 ccaaaaacaa caatgaagtg ttctatgtgc taacaacata gctttaaaaa aaataaaata 113280 aaataaaaca aaattctgca tttttataaa acttgataaa aaatagtatt tcaaactgta 113340 cagtcaccag aagtacacag ttatcaaaaa tgcacacact tcacttggca tctccagcac 113400 cttcagcttt ctgcgcctgg tctgttttgg catctccatt tcctgcaggg ttatttccct 113460 ccttgccagc atcagctttt ccctttttcc ctttgggtac cttctctccc ttctttgcag 113520 gggcctttta ggcgtgggct ctggctttgg aggagcaggt ttagcagaca acctcgcgga 113580 tcttctctgt ggttcgtcct tcaccttggc tttatctccc ttagcatccc cttcagcctt 113640 tctcttgggc atggtggcgg cagcgacggc agcgggacat aggtgctgga cgcgggacgc 113700 agcggcgcgc gggctttggt cggaccgggg gtcgttctcg cctcttcttc acactgctcc 113760 ggtctcaatc tcttgacctc atgatctgcc cacctcggcc tcccaaagtg ctgtgattac 113820 aggcatgaac caatgcgccc agcccagttt gcacttttaa taaggaaccc tggtgattct 113880 tatgtaggtg gtttaggaac cacactttaa aaaacgctgg cctcatggct tcaagtgaga 113940 catgttttgt tttattggta cagaaacaaa atgctgcgag gtcacacaga tagtgggtac 114000 agggctagag ttaaaatcca ggtgtgtctg acttgaagcc attctctgca ctgaccatct 114060 gcataatctt ggagcatttt gtttcttcat caagaaaacg aagatgatgc caggtgtggt 114120 agctcacgcc tgtaatctca gcactttagg agcctgaggt gggaggactg cttgaggtca 114180 ggagttcaag actagcctgg acagcataac aagaccccat atctacaaaa aatttaaaaa 114240 attagtagta gtcccagcta ctcagaaggc tgaggcggga ggatcccttg agcctgggag 114300 tttgaggccg cagtgagcta agatcacacc actgcactcc agcctgtgca acagatcgag 114360 accccatcta aaaaaaaaaa aaaaaaaaaa aaaaaaagac aaaaagaaaa gaaagaaaga 114420 aaacgaagat agtggcatct acttgttagg tcattactag gataaaatga gatgagccat 114480 gtaaagaggt tggtgagggg caaggcacca tagcaagtgc tcagtaaatg gttgctgtgc 114540 acacacagag ctataatata cttgcactac agaaatgcat ggaatcaaaa aaaatgtatg 114600 ttttctaaaa acagagttaa tctgctaggg acttacaaac ctcaagtaaa ggattccaca 114660 agaacgctct cagattagtt ctacagtcaa caaatgcgta ttgaggggat tttatttcct 114720 ggtataactc aaagacttcc tctgagggtt tttcagctga gtgcaggaca gcagctggga 114780 gcacagaggc agtccacatg agcattttct gggcttgcca taatgctttt gactgggaag 114840 agagattttt ttaaaagatg gcttttcctg gatctttttc aatttgtagt aaaatacaca 114900 gtgggattct gaacacatct cattttattt tcctgctgca tacacccttg gatgctcaat 114960 aagtcactgc tgactggtgc caaagcctcc tgcctgctct ctttgattct gcttctgtgc 115020 cgtgccccgc agtctattct caacaagaca accagtgtgg tccttcaaaa atctagatct 115080 cttctgtcat ttcctattct tgaccccctg tgtctcctca tcacacttga aagtaaacct 115140 caagtcctgg ccgggccctc cttgggtgtg cccaatcagg tcctggctta cctcacatcc 115200 tcccactcca actcacatgc agccacactg gccttgctct gctctgaata cgccaaaaac 115260 atttccctct cagggcattt gcacatgtag tcccctctgt ttgaaatgtt ctctcaaaca 115320 tccacaggga ccgccctctc tctgcattca gttctctgtg gcttggagag gccatccctt 115380 cccaccctac ctaaaatacc taaaatagca cacccattct cagtccaagc ccctagctcc 115440 ttacactgat ttataacaca cattcaatat ggcatcttca gttagaatga aactccatgt 115500 gggcaggact ttgtctcact tactactctg tccccaggcc tagaaaagaa ccaaacacgg 115560 taagctctca aaaagcagaa tgagatgtca aatgaagtat ttctggtgtg gattggggct 115620 ggccacatgt aggatatctt gcttaattga caaatgattt atagagcccc tgttgtacac 115680 aaaacatggt gccaaggcaa aagatgagta agaccaagtt cctgccttca aagagttttg 115740 gtcttgccca gtgctgtcca gtagaaatat aacatgagcc acaaatgtaa tttaaaattt 115800 ctagtaacca catcaaacag taaaaagaaa tatgtaccat tagttcttat aattacctta 115860 ttttaaccca acaaatatcc aaaatgtcat ttcaacatgt aatcaatata aaaatgagat 115920 agtttacatt ctttctttgg tattatcctt taaatctggt atgtagttta catttacaat 115980 gcatttcaat tcagatgcaa gtttttattg gaaacccttg ttctatattt agagtccata 116040 aagtttacaa aatgtcagtt catattcaag ttgatccaaa catacttaat ggtttttgaa 116100 tcactgaatc aagttaaatt catcaaaatt aataaaagtc ctaattctgt ttctcagtca 116160 cactagccat gtttcaaggg ctcaatacca gtggctagtg gctgccatat tgtactaggc 116220 agtccaggtc taacagaata gatgaggtgt ataaatgtgg ggaagtgaat acattcatga 116280 agagatcata taaagaagag agtcaaagtc tgaatactgc ctttgtacac aacctcttcc 116340 tgacaggaac tgtctctttc tttaggactc aatttatatg tcagcttttc taggaaatct 116400 tccatggtac ccccaaattg ggttagttcc cctcaagtgt aagatcccag agtaccctgt 116460 atgtctacca tcttcagttt gcccttctat gtttcaattg ctctctatat atttatattt 116520 gcatttcccc cgagtttata aacttcatgt ggccaggagc tgtcactgtt aattcctcaa 116580 tgccagattc ccaataaatt ttgtaggaat gaatgaatga atgaatgaaa tggccaagac 116640 ttggaggaag aggaagagga aggactagcc ctgctagaat ggagaaacag gctgtggaag 116700 gggttccagg atggggcaag gaccagggat gaagatagaa gctcagttca tgcaaaagtc 116760 accagtggca aatgattctg aagagtccaa ggagatgtga gctaagaaaa cacgatcaga 116820 cttaataagc aggaagccac ttttatggga gcctacaaga gacaatcaga tcaacctaag 116880 ctcaatactt tttttataag ttcaaaaatg tattgatgtc cttggtttaa gagtttttga 116940 tattttaaat attgtgagta gactggattc taaggccgtg gtccagtttt aatgtctggt 117000 cagctgaggt tcttctagtt actgcagtct ccaccaatga cagctgttaa ctctatatgc 117060 taattgaaat tgggagcgaa ttgcagctgg cttggggcat ttcgtgtgat ttatgggcat 117120 ctgatctaaa gatacttaaa ggatggaatt attggagcca ggaagagaga tgtgattaat 117180 caaagaagac aattcagtaa atggcccact aaaacactgc agtataaact aaaacaggaa 117240 ctctagttcc atctattgag gcaggggagt gtgtgtgtgt gtgtgtgtgt atgtgtgtgt 117300 gtgtgtgtgt gtgtatgtgt gtgtgtgtgt gtgtgtgtgt gttaaagggt ataaacagga 117360 tatagaaggt tttttttcca gcatatagag attatggttg ggggattctg aaggacatat 117420 atttatcttt taagtttgct tacaatgtac ccatcaccat caggaaatgt caagttccta 117480 gccctgtgat ttcatcctga ggactcctgg gagttagggg aatcaccatg tgggaaaaaa 117540 aagacaatac cttttattca aatttttaag ggccagtgtg agttgtcatc tttccatatt 117600 cctgagtatt cattcatttt tatcataagg cctgtgggtc tgcggttacc tttgtcaaaa 117660 ttactggcac taagaaacca ggatgcaagt taaaagttct cagtatttat ctaaatagaa 117720 aaataagtga ttatcggtaa catataagtg attgtcttga ataattgttt atttgtgcct 117780 tcagagttaa cctagactta tgttcctcct gtccagtgga ctgtcttatt tattgctgaa 117840 gacctaactc tgacaggcag ctagacattt tttcagtagc tgtggaccat taaaacaatt 117900 ggtcagttaa gtcctttcag gaggaaaatc tctgacttgt ttgtaactgt gtaagtaggc 117960 accttctggg tccacacagc acaaatccta aatagcaaca cacacactca cactcacact 118020 cacactcaca cacacacaca cagcagaaga gaaattcttc ttagaaaatc aggttaaaaa 118080 gggaagaggg aaaaattatg cccagcaaag caagacatag tggtgctgct tccctcccca 118140 tagacactaa gactcagtgt ccccacctct gtcaccctcc cctaccctac atatacacat 118200 gcacccatta acatgcatac atgcttgctt tttccacact aacgcccctt cttgtgtcaa 118260 ctctaaagtg aaaagaatct ggggcctaag ttagtggaat actcacagaa atggaagcca 118320 tggatggatg gtatgggtgg aggtacgaag agattcgggt ggcatctttg cttttcctct 118380 gaaactatca aagaaatgat taggaatgat atatgtgctg agagatgtat tgatgacctt 118440 tacagaatgg aaattgaaga accagagatt aaagattaga aactcaaaat cttttagccc 118500 cagagtgtcc tagttcccta attggtaaga caggggccag tgaggcggac acaccttgaa 118560 gaaggactaa ctaaggatca cagtgctgta ttgaccttag caaatgctac agaggagagg 118620 aaataactga gccagaacaa tgattgtgtc ttatgacctg ccgtgtgcaa cagatgggat 118680 gctaaacact tacaggtact tttaattgaa taatgctttc ccgtggccat atgctgtgat 118740 tatttgtatg cttcctgttc atgctttatt catctgtagc tgtcactcac ttaaaaccaa 118800 ctttcccttt ccatatctag ggattgaagt ggatactgtg aagtgtgaat gagaaataga 118860 aaaaacattg tttactgagt acagcttatt ttctcctgca acgtgattat ttattatctg 118920 tgtgttcatt tcagattatg aattattact gctggtaatg cctgttcttt cttccagttg 118980 ctgtgcattg gcacacagga gcgaatatca aatgccaagg ggagggtaat acaacatcgg 119040 ggaactaggg taattctaac aatctattgg gaagaaaatc tgtgcacttg aattttgaat 119100 ttaagtcaaa gtttttaaca attgtggacc aaaagtagtc tttctttttg tattaaaaca 119160 gaaaacagtt gccccaacca aagcctctac aatgccatat cattagcttc tgagattaaa 119220 tttgacaata taagattaca tgcatgctaa tatttcataa ataaataata aggcatgcaa 119280 aagtcagctc tcagccatcc cttcctcacc tggcaaggta tttgatgaca gtgattatta 119340 ggtgactgaa agagaataag caaggccgta atgaaggaaa gaaattaatt gcccaaaacc 119400 tggagagttt ttggttcaga gctgctaatg atcaagttat atgcatgtcc tatttgccat 119460 taaaatgctg caaaactgga aatattctta atggaagaac acactattgt gtactttggc 119520 ctgccacgag catcatctag aacccctcaa atgcatagca cacatgttca taggcagcta 119580 tgttagtttg ctagggctgc cataacaaag tgccacagac tgggtggctt caactgcaga 119640 aactcatttt ctcacaaata tggaagctag aaatctgaga tcaaggggtt ggcaggtttg 119700 gtttcttctg aggcctcttt ccctggcttt tagatggttg ccttcttgct gtgttctcac 119760 atggtcattt ctctgttttc tgtgtcctaa tctgctcttc ttataaggac accagtcgta 119820 ttgaattcag gcccacctta atgaacccat tttagcttaa ttgcctcttt aaaagccctg 119880 tatccaatat agatctgagg tactgggggt tagggcttca atatataaat ttggagggga 119940 ctaagttcag cccataacag cagccatctt tctagcccag tgccctgcac atagtaggtg 120000 ctcagtgatt tttttttttt tttgagacgg ccttgctctg tcacccaggc tggagtgcag 120060 tggtgcgatc ttggctcact gcaacctcca cctcctgagt ccaagtcatc ctcccacctc 120120 agcctccaaa gtagctggga ttacaagcat gcaccaccac gcccagctaa tttttgtatt 120180 tttagtagag acaggatttc accatgttgg ccaagctggt ctggaactcc tgacctcaag 120240 tgatccacct gccttggtct cccaaagtgc tggaattaca catgtgagcc accacgcctg 120300 gcctcagtga acaatttttg aatgactgaa tgaaaagaat tagccagaag ttcctgtgtt 120360 gagagaaaag ggtgggtatt attaggagga gtgtggtgat gctgtggttg caactggacc 120420 attcagagaa aatagcaatc agttttttaa aaatatttat atccttaata aacctggaag 120480 aaaagtaaaa ttctagatcc cctataaaga gacaaggaga aaagacttgg atagtctctc 120540 tttgagggac acagttggaa gtatacacac agaagttctc caagagctgc ttctatcata 120600 tcttgtgatt taaagctgat tgcaagcaat tagcagcact tctgagttta tgaaaatgtt 120660 aagtgggtgg ttctgtagta gattttgttt actctagagc ccattgttta ttttaatgca 120720 ttcaaacaag aaatgcctga tgctagatgt aaaatgttta tcaattttac atctccccat 120780 agtcacaaag tttaaaaaaa gaccatgtga agtaaattgg aaagatgcat gatgcagtcc 120840 cattatccat taaatattca acattggctt tggaaaatat tgtagcactg taatttaaag 120900 ccaatttggg tagcatttta ataagatttg aggcttttga ataattgtgt taataccagc 120960 agctgctttt atttggttag taagatgact aattctataa ttaatatatt taacaaaaat 121020 atactgaaaa tatattcatc ttgtcattaa gatgtccatt tgtacaggtt tctctttaac 121080 atgaaaagat agtgtgagga tatcattgcc tccctctgct atgatgaaaa tgatggcagg 121140 taggtgtgtg ttgctgatgc gcatggctgt gtttttgtgc ggcgtcagtg gcagcactga 121200 gcgagaccca atcgaagact ctgcagagcc atggggtata taaatcttca gagtctctga 121260 ctcagtcaac catacagcag ctcaattttg tgccacaatc ctggtaatga gatttttcca 121320 tggtattggc ttaacattac ccctcaatgg gaacgtttta ttctgaacgt ttgctttgtg 121380 gaatgatctt tcagttctct ctcaaagtca ctcctccaca acatagaaat caggtgtgaa 121440 attcaaataa ttgaagacct taattcttca ttgtgtaccc aagtttcgag ggttactgcc 121500 ttggtaaaga aatgttggca ggtgctccat ctttgcctca ctctcttctc catgtcattc 121560 tggctgttct tttattgcgc gtcctgcttt ttttaaaata tgatgaagaa aagttcaaac 121620 ctaactggta aattaaataa aatacctaac attgctatta tttaacttga tttttgtttc 121680 ctacatgtgt tttaaaccaa aaaaatactt tatattttta aaataggaca ttagggctct 121740 cagcaatgtc ctgactgaaa tcttgcctct ctcctttggc acactgtggt ttattgttgt 121800 tgctgtcatt tcacgttgtt gttgctacct catttgcact aaataagcgt tctgtggaaa 121860 cagcatctga gccactgctt tccacccgtg ttctagatgc atccaatagg ttttactaga 121920 ggaaaggtgc tatgtgtcca cttggaaaaa ctgctagaga cactcttcct ctttgagtca 121980 taatggattg gggttcaccc ctgccccact gacagcagcc tatctgcact tgattgaggt 122040 tgtctgagaa gggtctccgc tcctgggtca gcattagaag cctggagtga aggaggaaca 122100 ggctacactg gctatgctgg ctcagtacta agcagaaaac gatatcatca agagcaaaac 122160 ccaattattt gtagccagat gggcacagat taatttctct tgaaatctca atggacgttt 122220 ttttttcttg tgattctctt tttataaaag agaagattaa aagggcttct tgcccttgcc 122280 tacaagaagg gcagccatca gctgtgtatt atgagagctc ttaagttact tacgatctga 122340 aaactaccag gtagacccca cacctccatt cctggggtcc tattgtttcc ccgacccact 122400 gcagttacca cagcatgtgc atcaggagca tttcccacat ctagggaaat gtagtagcat 122460 gttgattcag aactgattcc ctttaaatgc ttcctcttat gtcatgcatt attattcaag 122520 tcactcacta ttctgagaaa cattgtaggg ttccccacac ataaaacaaa gcatcacggc 122580 agccatgttt tcagactttg gcatgcaata gagtcacctg cagggctcat caaagcacag 122640 gttgtgtcca ggcatggtgg ctcatgcctg taatcccagc gctttgggag attgaggcag 122700 gaggatcgct tgagtccagg agttcaagac cagtctgggc aatatgacaa gaccctgtct 122760 ctacagaaaa aataataata ataataatta gctgggggtg gcagctcata cctgtcatcc 122820 tagctactca ggaggctgag gtggaaagat gcttgagccc aggagttaga ggcttcggtg 122880 aactatgatt gtgtcattgc cctccagcct ggacaacagt gtgagaccct gtctcaaaaa 122940 acaaacaaaa tattaaacac acacacacag aggttgccag gtctcacccc accatttctg 123000 attccagtgg tctggatgga accagggaat ttgcatctct aacacaaccc caggtgatac 123060 tcactctgct gatataagcg ccacacttta agaaccagtg gtatccagtg tcagagctat 123120 ccatgcacct attctttatt ttattgtttg aagttcagga gaccagggtt ttgagcttgc 123180 taacaaggtc tctttattcc agggactgac atcacttatg tacatgacca cttaatcatt 123240 aatttaatca ttaaactagg attgttagca tgaaaacaac agtacataac actgacatag 123300 aatttgagtt tacaaaacac ttttactcca ttacctcatt caagcctgca gacaagccca 123360 aagattattg ttattatttc tattctgcag atgacaaaac tgaggttcgg aattaggagc 123420 attaaccaga ctaattccag agctttttga tcttcaaact agagtatgca aactcactgt 123480 ccaaggagta tatgcagtga tgcttttttt gttgttgttt ttgagacaga atctcacttt 123540 gtctcccaga ctggagtaca atggtgcgat cttggctcac tgcaacctct gcctcctgag 123600 ttcaagtgat tcttctgcct cagcctccca agtagctggg actacaggct cccaccacca 123660 tgcccagcta attttttgta tttttagtag agatggggtt ttgccatgtt ggccaggctg 123720 gtctcaaact cctgacctca ggtgatccta cccgccttgg cctcccaaag tgctgggatt 123780 acaggcgtga accaccgctc ccagccgcag tgatacattt aagaaagtca attttaagaa 123840 actccacatc cataagcact ctttccttaa actgatctcc ctgagaacac aaccttcttc 123900 taatctatga aagaaagata taattttcac tagcatcaag ttagtaacct ctgggctctg 123960 aacaaagagg tgattcaaaa tattggtata gctggtgaga acgtgactca ctctaaatag 124020 gcaagtaata aatccttttg tggtcaggtg gtttcgaata tttttctttt aacaacattg 124080 aaaaagaatc aaataagcta gtagatcgtt aacaattttg atgatggata actaagtgaa 124140 ctcagagttc acagaattga gtgatgtagc cataaaagta aaactccttc catttgtatc 124200 tattatatga gtaagaatta atgacaaact ctgtctcatt ctatcaacaa gtaatattca 124260 tccatggata catgagccaa ttggggaaaa aaccctccac ttcatgttat taaaagataa 124320 atatccaata caactttatt ttttagttta aaaattatca aaaattgtgt aatacatatt 124380 gttatattga tcaattgtgt aataataact gcaataatgg ctcaatcgat agagttttat 124440 cacttaaaga gcttgtggtc acaggactct taccttttaa atttagatac atgtgtttgt 124500 tgcagagaat tataacaggt tgatcaataa aatcagaaaa agcatgattc atgcctaaaa 124560 atgtactgca ttaggttaga attctgtgca gatagtggaa tggaaattca aggtcaaaga 124620 gaaagaagaa acataaaatc tccttttgtg ggttgatttt tgttttttta tgttttttgc 124680 tttttggttt ttttgagacg agatctcact ctgtcgccca ggctggagtg cagtggcatg 124740 atcttggttc actgcaacct cggtctcccg ggctcaagcg aaaatctcct ttggatcaaa 124800 actagtctgt ggggttttca agtggccagt gacaggttca aaattgctat ggtacttaga 124860 ttccattggc tacattttaa agattaatga catggtttta tttaaagatg ctgttatttg 124920 caatatgcca gaaattttac catttaggat gggagggaaa tggtttctca gaattctttg 124980 ggattagatg agcataaagt gtggttccat attactcacc tctaattact caacacatcc 125040 agccgtttct gggacagttc tattcctttc cccaccacct ctcctcccta aggaaaaaaa 125100 aaaaaaaaaa aatctcacct ttccttacta cgaagatttt atctttgtga aatcaatgtt 125160 tcttagttat ttaatatcac cgagtcaaaa tcttttagct ttacctacag gcataattta 125220 acttagaaac gattatctcc atgtattatg agaagaaaaa ctagttcaat tttctcccta 125280 tcataagttt ttaaagattc tgctgcagac tattctcact gagtttcaaa aacttgaact 125340 gcttccctca ttagcacttg gcagaaggta gaaatgtaat gcattgtctg ggtgcaggag 125400 ctcacacctg taatccaagc actttcagag gccaaggcag gagcattgct tgaggccagg 125460 agtttgagac cagcctgggc aacataggga gaaaccacct ccctgtctct attattaaat 125520 atatgtgtta ttatatatat atatatgtgt gtgtgtgtat atatatatgt gtgtgtatat 125580 atatatgtgt gtatatatat gtgtgtgtat atatatgtgt gtgtatatat atatatatat 125640 agagagagag agagagatat taaaaattta aaaataaatg taatgcatct acacatccag 125700 ccagccaatg tggcataata acagcattat ttgcatccct acttttccat ctattccttg 125760 agagtgtcgg tgaaagtggt tctgagagcc ccagctggag tgagacagaa ggcaaacatt 125820 actcaaacct ccatgtttag ctttacaatg gtgtcactca cttgatctca ctcttttagc 125880 ccttatgaac tgcctatttt ccatggaaat ttccaggcat ggaaggtaac atctgacata 125940 gatatgccca aacattcaat gtgaaataaa tacacagact gcctctgatg tgttctgaac 126000 cataattcag atttcaaaaa tcttaatgaa tgaagcttct actttcccca agactgtcac 126060 taaatcttct catggcttaa ttaaatacta ataattgtac taaatccaaa atgttaatta 126120 tgtttatctc tgagtgatga aacttggagt tatttacttc ttttattttc tgtattgctt 126180 gccacataga catgtattat tattgtaacc aagaaacaat cacattgcta tctgagggta 126240 gaagaaaatg atcaaaagca tctgagacag gcatattggc tggggtccac cccttttcct 126300 ggtacaaatg agactctaag tgttgattta tatttatttt ttataaatcc tctctccacc 126360 ccacccccaa atggatttta ctttgtctac attttttaat actcactgtc aggaaatact 126420 ctgttaacct aagattttga gcttttccct attattctgt cctatggaaa gaagaacagt 126480 tattcatata atattaatga tcactcttaa tatcattaaa aagctctgtg gatctctcca 126540 gtaatttcta atacatcaag gtagccaaag attgttttgc agataaattt attttcctgc 126600 cagggatttt taaaaccata aagcattatc aaaatcataa agtatatgtt tctagctcac 126660 tcttttgaat ctgtctgttt tcccagactg gaattcacaa acgcattctg gttataatga 126720 tagcacgtgg tttggagaca ggatcagccg gtctggagac catcttccca tgtcacccaa 126780 gcctcccatg cagggaccct gtcttcctcc accccagaat gtccatggtt gtccctttcc 126840 tttgtccctg gttctagagt ccttttctca ctagtccttc aggaatgtat cacatttcct 126900 tcttaactgt ggccctagag tggtcctttg gccccactgt cctagcttgg atctctgtct 126960 gtctagccag gcatcccttt gctctattag attttcccta ccaaatccca gtccgtgggt 127020 tgccatcaag taacccttac atgtcagcac cgtgcctgtg aaagccaagc ccctcacctg 127080 gtgaaatcgt tatcagagtg gtcacgcctg gccaccttat gcgcccccct cacccctcag 127140 gttctgctga gccactgggc cactggagga gggactgcct cacctgagac tctcatctcc 127200 gcagccacct cccaccaaat gcccagttct tcctgcctct tccttcaact tggctcccat 127260 ctcagccctt ctgggggaag agggctcaga ttcttacaat ctccatagaa aggaaataca 127320 gtgtcaggcc agtttgcctg atgccttttt aaaaatcaat aaaggggaca gaatgcaaaa 127380 tatgtgctag gttgttttta tgaaatgcaa ggcaaaaaat gccccattaa acatttcttc 127440 tctgcagccc ttgctctcaa gggcacatgc ttgacccttg ggttcttaag actttcacca 127500 aggccacttt gtgctttgcc tgctctgcct gagtctctgg ccctcagagc tagagggtgg 127560 ggctggcctt ccagtctgta tctgtcccct gggggagtac ctttccaggc tgaatttaag 127620 aatgcagcaa ttgacttaga atgcagactc attgagtgaa tgcgagccct ggaggaaaga 127680 aaaatcactg tcacttctgt ggcccccagg tttgacactt tcagggcaac cccagagtct 127740 gaattgcttg gaacatccac tcacgcatgc aagtgaaagt catcagggaa gaggctttta 127800 atagcgcagc gtctgtgtgt ctcttgtgaa cccagggtgc cctcaagtgt tggctagggt 127860 taatcacaag ccgctgtttc tgagaacacc ctagaatgaa gcaaccctta aacgttttag 127920 ctttgtgact tgtgtccatc actcttaatt ttctgcagaa gctttttcct gcctttgtta 127980 cttctgtaaa ttcagtctat cagagggctt atgtggtact tttaaaaact cactttgatt 128040 tgcctttgtg caaattgagc tctgaaagag agcagtttcc agggaaattc atctcccagc 128100 aagaatcctg cctgctacca aatcatcttt taatctgccc cccgctttaa aatgtcatta 128160 tattttcctt atacagacct tcataaaact tacaaacact gtctagttta catcttcatt 128220 ttgctctcac aaggccaaaa aacttgttaa acagacttaa tgaacgagtt cctcttgatc 128280 ggggttgtaa gtttctaaaa ttgcatttga acttctacct gcctctagct gaatgaaaat 128340 tgcctccaga catctgaagt ctttttcttc ctttcatttt gcttaatcta cctctgtatc 128400 aaagcacatt tccattttgt ttaggccagt tgtcagactc taggcatttg cagattttag 128460 aaacctgcac aggtgttctg atgtgccgta aacttccaga gccactgaat tcagcaaaga 128520 aattccttga tgttcctact tattaatctt attaattaac atatatctta tatattaatc 128580 tttatactta ttgttgtcac tggggctaaa tagtctctat agctcgtggt ggggggctgt 128640 agtgtatgtg tgtgtgtgtg tgtgtgtgtg tagtgtgtgt gtggtatctg tatggtgtgt 128700 agtgtgtgtg tggtatctgt atggtgtgtg tactacatgg ttgtatgtgt tggggtgtgg 128760 ggggtgcgtg tagtatgtgt ggggtagtgt ggtatggttt gtgtgtgggt aatgtccagt 128820 atatgggtgt gtgtagtgtg ggggatagcg gtgtgtatgg gtgtgtggga gtgtcctgtg 128880 tgtgagggtg gtgtgtggtg tggtgtatga gcgtgtggtg tatgagtctg tggtttgtgt 128940 atgtgtgggg ggacatggat atgtgtggta tggggtgtct atagtgtcag cagatggtag 129000 tgtgtgtggg tgtgggtatg taggtgtagt gtgcatggaa gtgtggcgtg tgtgtggtgt 129060 ggtgtgtggt atgtataggt gtgtaggtag gtgtatgtgt gtgtatgagt gtgtagtgtg 129120 ttgggtgtgg acgcatgtag tgtgtggtat atgggtgtat gtagtataga tggtgtgtcc 129180 tatggtgtgt aatatatggg tatatgtagt atggatggtg tgtgtggtag gtgtatgtgg 129240 tgtgtgtgtg cagtgtgtgg ggtttgcaac atgtgtggtg tnnnnnnnnn nnnnnnnnnn 129300 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 129360 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 129420 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 129480 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 129540 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 129600 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 129660 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 129720 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 129780 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 129840 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 129900 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 129960 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 130020 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 130080 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 130140 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 130200 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 130260 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 130320 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 130380 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 130440 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 130500 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 130560 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 130620 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 130680 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 130740 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 130800 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 130860 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 130920 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 130980 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 131040 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 131100 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 131160 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 131220 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 131280 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 131340 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 131400 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 131460 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 131520 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 131580 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 131640 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 131700 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 131760 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 131820 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 131880 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 131940 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 132000 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 132060 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntgtt gttggtgtat aggaatgctt 132120 gtgatctttg cacaatgatt ttgtattctg aggctttgct gaagtcattt atcagctaaa 132180 ggagtttttg ggctgagacg atagggtttt ctaaatatac aaacatgtca tctgcaaaca 132240 gagacaattt gactttctct attcctattc aaacatgctt tatttatttc tcttgcccga 132300 ttgttctggc cagaacttcc aatactatgt tgaataggag cggtgagaaa gggcatcctt 132360 gtcttgtgcc agttttcaaa gggaacgctt ctagtttttt gcccattcag tatgacactg 132420 gctatgggtt tgtcacaaat agatttacta ttttgaggta tgttccacca atacctagtt 132480 tattgagagt ttttagcatg aaggagtgtc gaattttatc aaaggccttt tctgcaacta 132540 ttgagataat catgtggttt ttgtcattgg ttcggtttat gtgatggatt acatttattg 132600 atttgcatat gttgaaccag ccttgcttct cagggatgaa gccgacttga tcgtgatgga 132660 taagcctttt catgtgctgc tggatttggt ttgacagtat tttattgagg gtttttgaat 132720 cgatgttcat cagggatatt ggcctggaat ttcctttttc agttgtgtct ctgccaggtt 132780 ttggaatcag gttgatgctg gcctcataaa atgagttagg gaggagtccc tctttttttt 132840 attgtttgga atagtttcag aaggaatggt accagctcct ctttgtacct ctgatagaat 132900 tcagctgtga atccgtctgg tcctgggctt tttttggttg gtggactatt aattactgcc 132960 tcagtttcag aacttgtcta ttcagggatt caatttcttc ctggtttaga cttgggaggg 133020 tgtatgtgtc aaggaattta tccatttctt ctagattttc tagtttattt gcatagaggt 133080 tttatagtat tctctgatgg tagtttgtat ttctgtggga tcagtggtga tatccctttt 133140 tttgtttttt attgtgtcta tttgattttt ccctcttttc ttctttatta gcctggctag 133200 cagcgtatat attttattaa tcttttcaga aaaccagctc ctggattcat tgattttttt 133260 gaagtgtttt tcgtgtctgt atctccttca gttttcctct gatcttagtt atttcttatc 133320 ttcctgctag ctgtttgctc ttgcttctct agttctttta attgtgatgt taggtgtcga 133380 ttttaaggga tctttccccg ctttctgatg tgggcattta gtgctataaa tttccctcta 133440 aacactgctt tagctgtgtt ccagagattc tggtacattg tctgtttatt ctcattggtt 133500 tcaaagaact ttattatttc tgccttaatt tcattattta cccagtagtc attcaggagc 133560 aggttgttca gtttccatgt agttgtgtgg ttttgagtga gtttcttaat cctgagttct 133620 aatttgattg cactgtggtc tgagagactg actgttgtga tttccgttct tttgcatttg 133680 ctgaggagtg ttttacttcc tattatgtgg tttattttag aataagtatg atgtggtgct 133740 gagaagaata catattctgt tgatttgggg tggagagttc tgtagatgtc tattaggtcc 133800 acttggtcca gagctgagtt caagtcctga atatccttgt taattttctg tctcattgat 133860 ctgtctaata ttggcggtag ggtgttaaag tcttccatta ttattgtgtg ggagtctaag 133920 tctctatgta agtctttaag aacttgtttt atgaatctgg gtgctcctat atttggtgca 133980 aatatattta ggatagttag ctcttcttgt tgcattgatc cctttaccat tatatgatgc 134040 ccttctttgt cttttttgat ctttgttggt ttaaagtctg ttttatcagg gactagaatt 134100 gcaacccctg cttttttgtt tttgtttttg tttttgtttt gttttgtttt gctttccatc 134160 atctgcttgg taaatactcc tcagcccttt attttgagcc tatgtgtgtc tttgcatgtg 134220 agatgggtct cctgaataga gcacactgat aggtcttgac tctttatcca atttggcagt 134280 ctgtgtcttt taactggggc atttagccca tttcatttta ggttaatatt gttatgtgtg 134340 aatttgatcc tgtcattatg atgctagctt gttatgttgc ccattagttg atgcagattt 134400 ttcatggtgt tgatggtctt tacaatttgg tatgttgttg cagtggctgg taccggtttt 134460 tcctttccat atttagtgct tccttcagga gttcttgtaa ggcaggcttg gtgatgacaa 134520 aatccctcag catttgcttg tctgtaaagg attttatttc tccttcactt atgaaagtta 134580 gtttggctgg atatgaaatt ctgggttaaa aattctttta agaatgttga atattggctc 134640 ccactctctt ccggtgagta gggttccctt tgtaggtaac ctgacctttt tctctggctg 134700 cccttaacat tttttccttc gtttgaacct tggagagtct gacaattttg tgtcctgggg 134760 ttgctggttg ctcttctcag ggagtatctt agcggtgttc tctgtatttc ctgaatttaa 134820 atgttgacct gtcttgccag gttggggaag ttttccctga taatatcctg aagtgtgttt 134880 ttcaacttgg ttcccttctc cccgtcaatg tcagggactc caatcaattg taagtttggt 134940 cttttcacat agtcccctat ttcttggagg atttgttcat tccttttcat tcttttttct 135000 ctaatcttgt cttcacacct atttcagtaa gttgatcttc agtctctgat accctttctt 135060 ctgcttgatt gattcagcta ttgatacttg tgtatgcttc agaaagttct cctgctgtgt 135120 ttttcagctc caacaggcca tttatattct tctctaaact agttattcta gttagcagtt 135180 cctgtaacct tttttcaaag ttcttagctt cctcgcattg ggttagaatg tgctccttta 135240 gctcagagga gtttgttatt acccaccttc tgaagcctac ttctgtcaat tcatcaaact 135300 cattgtctat ccagttttgt gcccttgctg gagagaagtt gcgatcattt gaaggataag 135360 aggcattctg gttttggaat tttcagcatt tttgtgctgg tttttccttg tcttcatgga 135420 tttatctacc tttaatcttt gaggctgatg acctttgaat ggggtttctg tgtgggagtc 135480 ctttttgttg acattgatgt tactgctgtc cgtttgttag tttttctcct aacagtcagg 135540 cccctcttct gcaggtctgc tgcagtttga tggcggtcgg ctccagagcc tatttgcctg 135600 ggtattacca gcagaggcta cagaacagta aagattcctg cctgctcctt cctctggaag 135660 gttcttcccc aacgggcacc agcctgatgc cagctggagt tctcctgtat gaggtgtctg 135720 tcaacccctg ttcggaggtc tctcccagtc aggagacatg ggagtcaggg acctacttga 135780 ggaggcagtc tgtcccttgg tagagcttga gcgctgtgct aggagaacct tccttgtcag 135840 gatctgctgc tctcttcaga gcaggaaggc aggaacattt atgtctgctg aagctgtgcc 135900 caaagctgcc ctttccccca ggtgctctgt cctagggaga tgggagtttt atctataagc 135960 ccctaactgg ggctgctgcc tttctttcag agatgccctg cccagagaag aggaatctag 136020 agaggcagtc tggccacagc cactttgcca tgctgtgttg agctccaccc agtctgaact 136080 tccaggcctc cttagtgccg acagtgggaa aaaccgccta ctcaagcctt agtaatggcg 136140 gacgcccctg ccccccacca agctcgagtg tcccaggtgg acttcagaca gctgtgctgg 136200 cagtgagaat ttcaagccag tggttcttag cttcctgggc tccgtgggag tgggacctgc 136260 tgagcaagac cacttggctc cctggctgaa gcctttcaag ggtagtgaat ggttctgtct 136320 cactggggtt ccaggtgcca ctggggtatg aaaaaaacaa aaacaaaagc aaaaacaaag 136380 aatactccta cagctagctt ggtgtctgcc tgaacagttg cccaattttg tgcttgaaac 136440 ccagggccgt ggtggtgtag gcatacgaag gaatcccctg gtctgtggat tgcaaaaacc 136500 atgagaaaag cgtagtatct gggctggata gcaccatccc tcatggcttc ccttggatgg 136560 gaaagggagg cccccctcca ccgctccttg cactccccac tccccaggtg aggtgacacc 136620 ccaccctgct tctgcttgcc ctctgtgggc tgcacccact gcccagccag tcccagtgag 136680 atgaacagtg taccccagtt ggaaatgcag aaatccccca ccttttgtgt tgatcacgct 136740 gggagctgca gaccagagct gttcctattt ggccatcttg ccagatgccc tcaagactga 136800 tttttttaat catcactttt aaaatctaca aaacaggcta tggcagggga acctgaactg 136860 agtgtgttat ctgagcatat ctgaaaggca ggtggggtgt gcccggaagg actctacctg 136920 cagaaggtga gaggagcaac tagggagaga aagatgctga gaaaagaaag ccaaagagac 136980 aaatatggcc agtggcccaa atatggccat tggagatagt gaagtttctt tgcccacaga 137040 ttgtagttta tcctttttta ttttatttta ttttattgag atggagtctc actctgtcac 137100 ccaggctgga gtgcagtggc acaatctcag ctcactgcaa cctccgcctc ccaggatcaa 137160 gggactctcc tgcctcagcc tcctgagtag ctgggactac aagcacgtgc caccacgccc 137220 agctaatttt tgtattttca gtagagacag ggtttcacca tgttggccag gctggtctca 137280 aactcctgac ctcaagtgat ccacccacct cagcctccca aagtgctggg tttacaggca 137340 tgagccactg cacccaggca acatatgtga atcttgaaga cattatggta agtgaaataa 137400 gccagtcaca aacagatgaa tactgtataa tgccacttac atgagctccc tagaataggc 137460 aaattcatag aggcagagag tagaatagag gttaccagag catgagggta gggcaggaat 137520 gatgagttat cgtttcatgg gtagagtttc agtttgggat gatggaaata gttctggagc 137580 tggatggtgg tgctggttac acaacaatgt aaatgtactt aatgccacag aattatacag 137640 taaaaaaaaa aaaggttaaa atgggaagtt tcatgttatg tatattttat cacaatgaaa 137700 acaaaataag cagttaagta acttgcccaa gacacagagc tagtaatgag cagaatgagg 137760 attaacagtc aggtgctcca gttcagagcc tgcactgtct accttttcca gagacacttc 137820 caaactggga ggtggcacca gctagcaggc atctgttggt ccttgctgga catcaaaggc 137880 ataaatatgt ggcaaaagat tctccaaaaa gagaaacaaa tgtccaccac acggtgattg 137940 ggagggcgac attcctgaac acacgacatg tttcctggtc atctccagaa ataatagagg 138000 aagagactag acagctcata gtaacatgtg ggaatgagtg actgatgtgg ttattctaat 138060 tctattttta aaatatacct ggtgtagcca gagacatact agctacaagg gcaaaacttt 138120 acagatgtgt gggtaccgga aaatatttgg aaatttatgg ttaatgcata tatatgaggg 138180 agtctctgaa cctattctgg ttcaggaggc tgcctgaaaa gaaaagaaaa tttaatatta 138240 aaaaaataca tctatatgaa gtccaagtac aatatgatga tttttttcat tacttgatgg 138300 cctgtacctt tcatgccttt ttaactgtgg agggtatatc aaaatgtaaa gtattctttt 138360 gtatattcca ttctacttaa ggtgattcaa acaggttaaa tttttcagtc tctggaattt 138420 agcaggattt agcagagtat atattataaa caaatatgta gaacaggtga tacatgctgg 138480 gagagtggtt gtctacatca ctgtaaatat accaaagaga aaggcattga tgccaagcat 138540 ggctgttgca agatacaagt cggggaagct ctaaaagtga catttgatgt ggcttctgca 138600 atcagtagga attttccagg cagacaacag gggtgaggag gggatattct aagcaaagga 138660 aagagcctgg gctgagacac aagggacatc acattgacag tgggtacagg caacagaaat 138720 taatgcagta ggactgaagc tgtgtttctc attgtgcaga cctgcataag aaccacttgg 138780 ggtgcttgtt tggatcccac acaagcagat ccctgggtcc cagcccagcc ctcctaagtg 138840 ggacatttct agtaacagag gaggggaata cattttcaac gatatcctcc tcgtgattct 138900 tagcacacaa aagtgcagtg gaggggatca ctgagctgga ctggggagcc cctggttgga 138960 aatgagtgtg gagaagcagg ctggggcaag gttgcaaagg atcttgtact ataggcagtt 139020 gtatttggaa ggtagacaga aaggagataa cctgattgtg ggcagattct ggaaaaggtg 139080 ggagggggaa gggaaagtgc agctgcagtg aagcaagttt tcttattgag aggtgagttg 139140 tgatggtgga tgagggctga gagacaatga atactggtga tactggattc acggggctcg 139200 atgatgcgtg agaactgagc gtggagtcca ggaggcagga gcttcgagag acctgaagag 139260 aacactgatt cccagcattc tcactgcagg tcttgtggaa cccagtgtgg gcccgtcccg 139320 ctgcatggct gcagctttat tggtgtaaac ttgtagggac atgtggttag tgccaggtgc 139380 ttttattaag gatgctgttt ccctattcct gaggactcac gggaattttg agaaccaact 139440 tctcccattt ttatgagaca agagcgcaaa cgctttagga aatatatggt tattactttt 139500 ctgtggtaat aaccatttat aagctgcggg tgttttcaga aagtatttga tggagaatgc 139560 tacactctta ctaaaacccc aaggggaaca tcacttcttt actcttttta ctctttcatt 139620 gtgtgtcacc tttacttgtc aacttcaggg tacataaagt tgcttccagg tgcataaaaa 139680 gagtatggga aacccctata gtttaacttt ataacaaaaa agtatattca aaagagctta 139740 taattgcata gcaaaaacta acacaaaaac aaaacacatt catgcaggaa taaaaagggg 139800 tctcctaaaa gtcggttttc acacctctgt tagaaaacga agtttcgaag tatcctcaag 139860 cttttggtct attacctgtg agtacctcac ctatgacatg gatggacact gtgactagtt 139920 cacagcaaga tttctcctcc taggcactgt tgacatttta ggctggatac ttcttgtttg 139980 aaggtggtga gcctgtcctg tgtattataa gatgtttagt agcatccatg gcctctacct 140040 acaaaatgcc agtagtaacc cttcttccca aggtcataac aattaaaaat gtctccaggc 140100 tttaccaaat gtctcctggg gtatagttct cagatgtata aaataaaaat acaggaccct 140160 tagttacgtt tgaattccaa ataatggaca aactttttag tagaagtatg tcccaaatat 140220 tgcattggac atacttacac taaaaacgaa tttttatcag atgtaactgc tggcctgtat 140280 tttatttgat tgccctacct gggcagccaa caagatcgtt gatgtacgaa acaggatgcg 140340 tgtgaggtgc ctggcacatg gtgggtgcat tataaatatt caaggaatct gctgcgtacc 140400 tttgaatcca ctttacccgt gtccctgctg tagtctgcaa ttggtactcg agcctgccta 140460 tcctttgcca aaactgtcat gagagtgggt gtgaggagat agaaagccca catttgaatc 140520 tgcaagttct gggaactagc attctggtca aagctggttt tacatccacc tctgccagag 140580 ggagcatcaa tctgcctatg ctcgagcact ttgtcactgt ggtctccctg agtcattaga 140640 tcatttctgg taagtagcat tcagagaagt gatagtgaat gaaaaactgc tgcattagtc 140700 ccagactcag tcactctgca tttgattttt ccagcagcat agccctagaa aaccgttcac 140760 tgggatgcag caggaccaat catttccaca ttcagttgca gctaggaact tatttctgag 140820 ccaagatgaa tgacccaaat agcctttcca gagagcagag gtgaaattta actctgggtg 140880 gttgcctgac attttgagac aaaggagaaa ggggatgggg aatgaagaaa aatagcattt 140940 gaatgtctcc tgcagggtca taaatctgga tgggcaaaag tggattgcat ctctgccaac 141000 aatttaatta atttgggctt cttttaatct ctatgattca tataacctga aggaaacatg 141060 ctttaaagct gtattgtagg aagagcctcc tttgagttac atatcagtat attaactctt 141120 ggatgtttta tttttccaga aggaagtcta agttgtgtgt gggggaagta ctaggtttgt 141180 tcacaaaaaa atgcattcat tgctttgggg gaaagccccg ttcctgtctg actgtagtga 141240 gatgaatcat tagaagtgta atcacagccc aggggaggaa aaacaagagt aaaggattct 141300 gatggctccc agcaaagcct gagagtgggc tggagatttg catttgctta ttcttgctgg 141360 tgagcctttg ctgatcatct ccaacttata aaaagttgca agtctatggg aaactgtttt 141420 gaaacaaggt aatacaaaat ggcagctatg acactacaga atagcttggg gacatatcta 141480 gctgggatgt gtattgcgtt ggggcaggat gtatattgtg ttggggctca aacgagcaga 141540 tgcgaagcct tttatggctt tgctactcaa cagaaaggtg tttcattttt taatccctat 141600 gatttattta tttatttatt tttattattt ttttttttga gacagggtct cactctgtca 141660 cccagggtag ggtgcagtgg tgcgatctca gctcactgca gtctctgcct cccgggttaa 141720 aacacttctc ctgcctcagc ctccctagta gctgggatta caggcaccaa ccactgcatc 141780 cagctaattt ttgtattttt agtagagacg gggtttcacc atgttggcca cgctggtctc 141840 gaactgctga cctcaggtga tctgcctgcc ttggcctccc acagtgctgg tattaccagt 141900 gtgagacatc acgtctgacc taatcgctat gatttttaaa agtgaaatta tcaggcaaga 141960 agaaatgtca aaagggaaaa aaaagaaata aaaattaaac gattccattt tgtgtcttca 142020 gtggtaattc aagtaaccat gattttctat gtttcttcct cctcaatccc acagtttggg 142080 gctcaccaat tccttaacat acgctctgcc aaatattagg gtgccaggaa gtatattggc 142140 aaaggaatat agatgtgtct tgattttata acaggtatat tgttccattt tcagtaacgt 142200 gatgggctat gttattggga ctacccacaa aaaacaaccg aaaagagttt aacatgacaa 142260 ataggaggaa aaaatcagtg agtgtataga tgatttgaac aacacaatca acaaacttga 142320 cctaatagat gtatgtagac actgcaccca atacctgtag ggtacacaat cttttggagc 142380 atacgtggag catttataaa aatggactat atactgtgct ataaagcaag ctccagtgta 142440 tttcaaaaaa actactaagc atgtgttctt tttttttttt tttttttttt ttgtattttt 142500 agtagagaca gggtttcgcc atgttggtca ggctagtctt gaactcctga cctcctgatc 142560 tgcccgcctc agcctcccaa agtgctggga ttacaggcgt gagccatcgc gcccggcgta 142620 tgtgctctta tcacagtgaa attaagccaa gaaatcagct ttttgaaaat taggaaacat 142680 aataaatatg tttctaaaaa ttccagtagt cacattttca tccactgtac ttggagggag 142740 agtatgatgt ccccttgctt gaggcacaag gaaccagaaa ttagagctcc cagtcattgt 142800 agttgcatgc atttccccac ccattagaat ggaatttctt tgacaaaaat gacaaaactc 142860 taagggtcat tttgggcctg agtcatgagc taagcagctg tttcaattgt caagtgttaa 142920 atgatttgtt ctggatcaga gactataagg caagaaatgg ggctaggaaa aagaaacagg 142980 ttacagaggt caacaagtta gcagaggtat acaagtgtgc cctttgggca tcagcaacaa 143040 agagcacctg ggctgacctc tgctgtctta atgctggaaa tgttatagcc cgctctgtaa 143100 tctctccccc ttgcccccgg gagaaaaatc tttcacctaa caatggattc cactagaatt 143160 tttgtattgt tatcacaaaa atcttctagg aataaacaga gagcttttgt tgatcatgag 143220 caagatcttc ctggggccat gtgcaaacag caaaaagaaa atttgatctg gaagagaatg 143280 agaaccatgc agttgtttgg aatactattg ttgggctttt tgggttttta cggacagcta 143340 ctttgaaaag cagattgact aagttgctaa tatgtgaaat aagcaccact attttgctgc 143400 aagtcctcta tcaacttttg gacacttgtc tcaggactaa ctgcagttta gaaggtgttt 143460 tgttttgttt tgttttttgt tttgaaagta tttcgtttct tgccttagat ctatacttga 143520 aaggaccttc ttgtggcctt atctggtaga gacacaagat cttagaatca gagagaactt 143580 tagttctgga atgagcttta gaggctagta ttccaacttt ctacccaatg taagaattgc 143640 caatatttac ttttgtttgt ttttcagtct cctaagactg tatgtgttca taaaatcagc 143700 ataattgatt tgttattatt catccacaga catcagctaa tataatagtt tatatataaa 143760 tacaaaagta tacatttcac tttaaataaa catttatgtg agtaaaatag agaaggaaac 143820 ttaatgtgtg cctatttgtg ctttaagata ataacatgaa tatatacaat gtctatttat 143880 agactaatga gctgacttaa catgcattta tgaatactca agtcttcagt tgcatttggt 143940 caacatttga ctatcctaca ggctctttta gtataaattt gcataaaaat cttttacaaa 144000 aaagatatat atatatatat acacatatat atatttttaa cttcaggctg attgacctat 144060 actatcataa cattaatact atattattat ctatatacaa aagaaaatcc caaaggacct 144120 ttgcttaaaa tatagacaca cacacataca catgcacatt tcatgctgtc agcttttttc 144180 aactctcaga ctcatcattt ctctcactgg agtttgggaa gcaccatgct tgagtcagtc 144240 ttacatgttg gccccaggaa ccctttctga aaagttcccc tcattcactc tttgcatatt 144300 tggggcactc acagaagata ttccagaaaa aaaaatgacc ctaaaaaaat aagcccatga 144360 atttgtgagt ttcaaaagcc accagaaaat gtgcttcttc gaaagcacgg ttagggtcat 144420 aagtcaaatt caggaagcag aggcataatg tgacttcctt ccaccgctgg ctgctcctct 144480 gcgcattcca aagggcaaga ccagagcttg gaaacagaaa tagaaaaact cgcaggaaag 144540 gttggcttgt tgctcgggcc tgctcttaaa gacagatgac tattggagtt tgttggaatt 144600 taacccctgg actctctccc tgggatgtat aattggctct cacttcctga atgcatgatt 144660 atgtgctgca ggcagatcgc tgccaattat ttttattgtg attgctttgt gcatctctgt 144720 tggcaatagc gttcacagga tttcagctca aggggaacgg ccacagcccc aagggtccaa 144780 gattagaata tgactgcggt tataggtcaa aagattaaag gtctgtgaat attgttctct 144840 ttttcaccca gagataccag gatagaatct ggcactttcg ccttggtatc cataagtgag 144900 acctgtattg tcagtgttta gatttgtcac aatcagaatt aacattcgtt tagttcagaa 144960 aaatatgtaa gtattgaata gtggcacaat tataatttga ttctttctcc tctctcttct 145020 cccatcaggg cctgtgtctc aaaaaatgta aaggcttgca actctttgaa aagctttctc 145080 ttttcctttt tgggtaaaaa gttatacaag acactagaaa attcaaacat taaagaaatg 145140 tcaagtgaaa atcttcagaa tggaggacta taattttttc aaaaagtaaa agtcctcatg 145200 agcttctcct ccctaccctg agtaaccact ttaaatagtt tagtgggttt tcctcctagt 145260 cattttctag atatgtttta tgtatatata taaaaactga tatgttttat gtatatatat 145320 aaatgaaatc actattattt aaaagtgctt ttttcactga gcaatgtcac ttggacattt 145380 tccatatatc tgtttagaaa aatcgacctc attctttccc aatagttata tgatattcca 145440 ttgtaaggct ataccatagt ttatttaact atctcccctc catgtgcccc cctccaccta 145500 ctacttggcc ttttttttcc tttttctcct tctttttttt tttttctttt tacaaattga 145560 gaaggagatg aaaaatctct gctggtatta gcaacaggaa ccatccagct gcatctggtt 145620 aaatctggtg gagtcacaga tgcatgacaa cctgagtttc cagccaagga ttaatgtttc 145680 atgcagatta catagtattt atattttaaa cctgtgacat gttgagggga taaaatttta 145740 tatctgtagg aacaaaatca gaaatttatc tgatcattgt cctcttccta agcttgttgg 145800 gcccttgaaa aaatggattt tgggcccctt tttaaagaaa attttagttg gccaggtgcc 145860 ttgtgagtaa agaagaccat agacaaatca atacaggtga tttacttctt agcttaaaaa 145920 ttgatctcac atatgatact taatgttttc gtttagaacc ttgggtttgt gtcttgtacc 145980 taaaatctgc attcctacca aaaactattt cattaataat atcacataac cgacagtcaa 146040 atacagatca gtaggccagg tgcagtggct cacgcctgta atccccgcac tttgggaggc 146100 cgaggcgggc agatcacgag gtcaagagat caagaccatc ctggctaaca cggtgaaacc 146160 ccatctctac taaaaataca aaaaattagc tgggcatggt ggcgggcgcc tgtagtccca 146220 actactcggg aggctgaggt aggagaatgg cgtgaacccg ggaggcagag cttgcagtga 146280 gccaagatcg tgccactgca ctccagcctg ggcaacagag cgaaagtctg tctcaaaaaa 146340 aaaaaaaacg aacacaaaca aaaacaaaac aaatatagat cagcaaagta ggtgtcaagt 146400 ccccagggga tcagggacag agcccagaca agaacatgaa agccagcatc cttgtatgtg 146460 tgtcacatac ctctgccttg gcaccttgaa aggtttcaga cagtcaggca actggggagg 146520 cagctgtttt ctgcggtcac agagcacatc agcttaaggc tggtaaaaag ctttgattat 146580 tattcatgac atataatact tttattagct ccacactgat tagtgctgtg ctagaactgc 146640 tttctgtaaa attgagatcc ttccttgcat ggaacttata ttatctgtag ataaattcct 146700 cggtatgaca tttgaatgtc ttaacatttg agtatcttaa caaagtccaa aggataatta 146760 gaatcatcac tcatacaaga catattttga agacaaaaag gtgtggtgcc aatataaaag 146820 gttcatttaa aagaatccat tttactaact gcaggtaagt atccaaaagc aacaacccac 146880 tttcccagtt gattgtggct tgttttccca ttcctgtcct tattccacgt ttgctgtctc 146940 tccactttct gattttgtag tgtaggcttt gtgtagctct caaattcaaa tttaagccct 147000 taaaatggag ctcaaaagca ggtcctgaga aattgatggc tgagtagtga tggttgtcag 147060 tgttttacac cttcgttctt atatctatgt ttaacccaga gaatggtatc atagttttac 147120 aaagcatttg cagggtcatt tcttgaggcc ctcatggtgt ctatctctca ttcaagccac 147180 aggttatcac ccatcagcct ttgaccctgc cccctgctat gggacatgcc acaaggcagg 147240 aggagtaccc aacacccttt ccaggactcc ctctcagggc tcaagtgatc ctcccacctc 147300 agcctcccaa gcagctggga ctacaggcat gtgcctggct aacttttgta tttcttgtag 147360 agacgaggtt ttgccacttt gtgcaggctg gtctcgaact cctgacctca ggtgatccac 147420 ccacttcagc ctcccaaagt gctggaatta caggcatgag ctacagtacc tggccttttc 147480 tttttctttt tttttttttt ttgagacagc atctcactct gtcacctagt agctatgacc 147540 acaggtatgc accaccacaa ctagcttttt tcttcctttc tttcttttct ttttttttta 147600 gagatgaagc cttgctatgt tacccagtct ggtctcaaaa tcctgtcctc aagcagtcct 147660 gctgccttgg cctcccaaag ggttgggatt acaggtgtga gccaacacat ctggccttgt 147720 ctgttaacat atctgccttt ttcaatggat tataacttcc ttgagatcag ggaatcagtc 147780 tccatatcac agcattatga caggcacata taggcactaa agtaggaatg aatgaacaaa 147840 aagtaaatga agatatcact aggcattagg gaaacacaaa ttaaaattgt gatgagatac 147900 cactagatac ctataagaaa ggctaaaatt taagaaaaga aacctaacaa gtgaagggaa 147960 agatgcgaag cagctagagc ttgcatacac tactggtggg aattcaaaat ggcacaacca 148020 gtgtgcaaaa tggtttggca gtctgctgta aagctaaaca tgagcttatt atgtgactcg 148080 accattccac tcctacatat ttacccaagg aaattttggg ggcacacaga aacctgtatg 148140 aaatgtatat agcaatttta ttcataatca cccaaaactg gaaacaatct aagtgtcctt 148200 caactggtga gtggataaac taaatgtgat atatttacac aacggaatac tacaacacaa 148260 tgaaaaagaa caaaccacag gtatgtgcaa cagtttgcat aaatctcgac tgtaacgcaa 148320 agtgtaagaa ccagaccaag gctgggcaca atggctcaca cctgtaacct cagcattttg 148380 agaggccgag gcagaaggat ggcttgagcc caggagttca agaccagttt ggataatatg 148440 gtgaaactcc gtctttacaa aaagttagcc gggcatggtg gcgcttgccc gtggtcccag 148500 ccatttggga ggctgaggca ggagaatcac ttgggaagcc aaggctgcag tgctgagatc 148560 acgccactgc acgccagcct gggtgaccta ggagacctgt ctcaaaaaaa aaaaaaacag 148620 attccaaaag ctatgtacaa tatgattcca tttatatgat attctagaaa agacaaaact 148680 atagggacag acaagagatc agtgattgcc aggggttagg gctggaggaa gaggtgactc 148740 tcaaggggca gcaggaggga atgttttgag gtaatgaaac tgttctgtgc cctgataatg 148800 atggtggtta catgactctg catttgtcaa gctcatagaa ctgtataact aaaaacaaat 148860 aaagttttac tctatgaaga taatacaaac ttaatttgaa aaagtgaatc aaggttattt 148920 tgctgtttag tgtagattca gaaagaaaat aatcaaaaga aaatctgggt ctgtggtcct 148980 gtaacctgca gtgattttgc ccatgctcat gtacacattg tgtgtgtgtg tgtgtgttca 149040 acaaacatta aaagaacact tcctatggac tagcacttgc ttaggaggtc ttgatgagtg 149100 ggaaaagcag ggttcctgct ctcttggtgg gagagggaca ggtaatatgt aaacaacaca 149160 tgaatatgac aacttcagat agtgataaga gctgtcaaga caataaaaca ggcctacgtg 149220 agtgggattt tgtgtgtgag cctctctgag gagacaccct taagcgaatc ctggaaggta 149280 cgaagcctgc agccatgcat gcaaatactc aagtagagct gtccagacca ggaaaacatc 149340 acattccaag actcctaggg gacaacattg aaaaccctca cttcaaaact cagaaagcct 149400 ggctaaaata caacaaacat cctttctatc ctttctcatg cgtggctggg cttccaggaa 149460 aaaggcttag cccccaggat gggattaagc tctatgttca gagaactgga agcaggtcca 149520 tgggactggc tcaagccagt tagaggacca gcagaagatg agctcagata catgaatagg 149580 aagcagtcta cataggcctt aggtgccacg atgagacatt tgggtttcat tccaaatgta 149640 ctcagagctg tgggaggctt tcaagtgaca gagtgaagtg accttattga cattttaaaa 149700 ggataactct ggctgctata ggaagagtgg actatggagg accaagagtg acagggagac 149760 caaacaggag gctgttgcta aaggtcaggg ggaagggcca cgtcggccag aacctgtgca 149820 gtgccatgga gatagggaga agtgagtggc tttgaggtag attttagagc tggaacttgc 149880 tgatggattg aacattgggg attgtgaggg aaaaagaaga tgaggatgag gtctcagttt 149940 ttgacacgtg tatctggggt ctgacagtgc tgaaataggg aagggtaaga gagaaacaag 150000 agttttcctt gatagtgtgg caggaaacca aaattccatt ttagacatgg taactttgag 150060 acacccatta ggcatctctg tgaagatgta agtaggcagg tcgaggtata tttctggagc 150120 tctgggaaca gaaaaagaat gtatttcaga gacagtggca tgcaggtggc acttacagtc 150180 ttggagatag attagtgccc ctagaaaggg cctgtggaga gaggagatga ctaatgacca 150240 agatttggac tgtgccaacc tttgggggcc agtcagagga gccagctaag gaaactgaag 150300 gaaagccagt gaggtgggaa cgaaactagc atgtagtgat gcagaagcca agggaaataa 150360 gttttacaaa aaacagtgga atggacagct atacagaatg atttagagag tttgattaga 150420 gtgcaaccat taaatatgac aaagactggt ggccttggtt agcgcagttt ctgtggaatt 150480 tgggaacagc ctgtttgggg tgagaatact gggggagaaa gtggagactg gctatagacc 150540 atccttttga ggttttactg taaaggggaa ctgaggaata gcagtgcagc tggagaagcc 150600 tgtgggagtg gaggaagggc tctttctcag gtgtcagata gagatatgct gatagaatga 150660 cccatcaagt aaagcgagag aacatgacga tgagggttgg agaggaggaa cagatactta 150720 caggagttcc gtccttgagg gaaagagaag gatgagctcc accgcccatg tgagcagggt 150780 ggccttagct aggcacatgg acggctcatc agttgtaatc aaagagaaga cagggagatg 150840 gaacaggggc aggtggatga atagattcaa ggatgagaag atgaggagta tgtagttctc 150900 tctgtgttta tttcctctaa gaaataggaa gtaaggtcaa gtgccaggag tggagttggt 150960 tgctggagat ttgaagagaa atggaaaaag gtataaaatg gttttggata ctggggaaaa 151020 aaagcatatt acaaacaaat ggagagagaa atgagtggac aacacaccct tagagagaga 151080 gagacaccac cagcatgtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 151140 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc 151200 cctggcccag acaggctcat cctgccaagc ctaggtctaa gcacaatctc agcttttgga 151260 gccagcagtt gcagccaggc cgaattcagt ttttctggaa ttcagttgtt ctgacccaag 151320 ttcagccggc aagtgagggt ccgctgtgtt cctggtgctg agagcgcgac agggtctgag 151380 cctgccagga tgacaggatc ctggtgatgc ggcccaacag gaagcaaagc acagggaggc 151440 agaaatgaga aacagtatct tagcccaagt tctaaatcag tcggcccctg gccaggttaa 151500 gtaacttagc acgtgtaaag actaaaaaaa ctaaagcagt agagaattac cttatacaga 151560 tgacaagata tggacaacta agtgagaagg tatcacaact aggtttaata gaaatcctta 151620 aaaaaagtaa gccgacaaac agaaaagaca acaacagtga aattcaacag aagaaaagta 151680 atagactctg atgaagatga cgattattga actaaaagtt ttcatagact agaacttaat 151740 ggaacgattc taggatggaa gttaagatct gatttaaaat ttactttgtt tattgtctat 151800 atgccttttt taaaaataaa cttgttatgc aaagtaaaaa aaaaaaaaaa ggcagggagc 151860 ctgaagtttt atagagaatg ctacggttct ccaacttaac ttctatcagc caagttagca 151920 gtagagcttt aactgtaaga agaagtgtat tggtttctgt tgctgctgta acaaatcgct 151980 ataagcatgt tggctttaaa caacacaaat ttgttatctg acagttctag agatcaaaag 152040 tttaaacgga ttcgcagggc ttctagagtc tccttctgga ggctctagga gagattatgt 152100 gttcttgtct ttccagcttc taccagccac ctgcattccc tagtttgtga cccactcctc 152160 tatcttcaaa gccagtagca cagcatcttc aagtctctct ccaaactaat ctctgcttcc 152220 ctcaacccat ctccctttct cattgtccct gcctccctct tttccttcta gggacatttg 152280 tatttacatt gagcccaacc atataatata aaataatctc tttgtctcaa gatccttaac 152340 ttaataacac ttacaaagcc ccttggcatg taaggtaaca tattcacaga ttaggatgag 152400 gacatcttta ggggtgaggg aatattctac cataagaagc ttcctgctgt atccattgta 152460 gtgactttct ctttggttaa atcacttcat cctatttgcc tttgaaaact taccttctga 152520 actaattgag gttctgtgtg agaaacatgt gaggggagaa gaaaaggcac acacacaata 152580 cctttaaggg taaacaagct ttatcccacg taaatggcaa tgcagatata ataagcaaat 152640 tgatataata agcaaatgat ataataagca aattgatata ataagcagat tgacataata 152700 agcaaattgc aatgggaagg agagaaggga aaagagattt acattcacca gactatggag 152760 gattcacccc cagactggga agtaacagcc tggactccaa agtcagccac tcatccatgt 152820 acagacaagg agaggtctca tgaagctttg gcgcagtctg ggaccccagc tctttttgta 152880 acaaattgtt tggcatgagg cccagtcatg agggcccttc tcgactaggc tcaaggaaca 152940 caaaaaagtc aacctgtttt tgtgattgtc tattgttttt caataactaa tgtgtaagag 153000 tcgattgaaa tagagatttc tctgaaacag tgctggatga atgcctcaag tggctcacat 153060 aacctgttct gggacttggt gaccattgtt tgtgtccatg ttcatctgag ttcaaattta 153120 atatttaact tttcctccac aattgattag aacagtggta ccagaggcta ggcacagtgg 153180 ctcacacttg taatcccagc actttgggag gccgaggcag gcggatcact tgaggtcagg 153240 agttcgagac cagtctggcc aacatgatga aaccccgtct ctactaaaaa ttcaaaaaaa 153300 ctagccaggc atggtggcag gcacctgtaa acccagctac tcaggaggct aaggcatgag 153360 aatcatgtga acccaagagg caaagattac agtgagccga gatcatgcca ctgccctcta 153420 gcctgagcga cagagtgaga ctccatctca acaaaacaaa acaaaaacaa gtctgggcgc 153480 ggtggctcag gcctgtaatc ctagcacttt ggaaggctga ggtgggcaga tcacgagatc 153540 aggagatcga gaccatcctg gccaacatgg tgaaacccag tctctactaa aaatacaaaa 153600 attagctggg catgatggtg tgcacctgta gtcctagcta ctcgggactc ctgctgaggc 153660 aggagaattg cttgaaccca ggaggtggag gttgcagtga gctgagattg taccactgca 153720 ctccagcctg gcgacagagc gagactccat ctcaaaacaa aaaccaaaac aaaagcaaaa 153780 gaacaatggt accagagacc ttcccttctc ttgccttcgt tgtttccccc tatactttat 153840 gaaatgtgct gtggcctgaa atttaaaaca ttgcatttgc ttaactcagc cacctaaagc 153900 ttttattttt ctttcagtgc cttaaagtaa taggatttag agcttttaaa aagaagttct 153960 ttatatgaag ctctgagatc aaatgtgaag tcaacaagtt gttttctctg atccatcata 154020 gggttttttt ctgcattttg gatatgtgag agttttgaac ttctgctaca taaaacatca 154080 tagcaatcat acaccctaca gtttatgata taattaatat ggtaatatac actatcttca 154140 tatattaaat tcccagagtc tactaatcta agattaatat agcatgtcat tgatgtttac 154200 atcatttgtc aacaaattca gcatttaaga atgagtaagc atgcacacac atgaggctgt 154260 gtgtggctgt aagattctgt ctcaagtcct ggcaggaaag actaaggtgt aacttcagct 154320 ctgtggcctt gaggaagtta catcaccttc agaatctcac ttggctccca ggtagacaat 154380 gagtaattat acagctctcc tggagatagg atgtatatta tctgattttt gttcacaaaa 154440 aggcatataa ataccaagca ttgctataaa ttaaaatttc acctcacata attcatctat 154500 gcctttataa atctcctctc tgttttcaat tagatttgat atgcctcatt atttctcatt 154560 tagaaccagc cattggaatg aattttatat tcatttgaat ggatataaaa tggtttgctt 154620 tcaaccattt tatgtttatt caaatattag ctttctttta gttcttgaaa ggatttctgt 154680 ttatatccca ttatgcttat ttttctatta tataaaaatt tatattttta taatatttag 154740 aagttgaagt gttctattta gaaatgtaaa attctctgga tctatttcat gaggtggatg 154800 aggagacatg cagcaaccgc caattcctaa taattgtact ggtcagggtg ccagcaggaa 154860 gctagtgaca ccctcaaaag gggccgtgga aaggtgttta gcgaagggac tctttatgaa 154920 gatgtgggaa ccattcagag aaagcaataa ggaataataa agcaccctgg atccacctgt 154980 gcctcaaaga gcaacaggag ggaggggtgg ccagaccctg aaaagagcgg tggctgctgt 155040 gaaagaaggc ccaggaaatg agagcacaca ctgccagacc tccagccagt cagggggaag 155100 ggagaaggga agcaagggaa ataatatccc aaccctcctt cactctgttc tcttgccttc 155160 cttcctttat tcaaacttag ctacaagcca gtgggcaagg gagccggttg atgcagtcca 155220 cagagggcca ttccctagca cacagcagag tgggaaagag tggagagtga tctggaagag 155280 caaacaaaag ataactagca cagtggtcta ttggaaaaag catcccaaat ttatgttggc 155340 ctttggggtt tggtacatgc atacctgcac ccccatactt aggccttgac ccctttggtt 155400 ccaagcacct ttcttctcag ctctgccatt atgggtctta aagctgagct ggccaccatg 155460 gatgatgccc agatcagctc actgctctgt ggatagtaag acttgactaa taagtccatg 155520 gttcgggcat cccgggtgat gttcccagga ggctgtctgg gaggagctct ccctcactct 155580 ccgtgtcctc cttttccagg gtggagcttc agcatgggat cggcctacca gtttcacagt 155640 tggcgtgtgt ttgtcatcgt ctgtgcactc ccctgtgtct cctccgtggt ggccctcaca 155700 ttcatgcctg aaagcccacg attcttgttg gaggtaacac ttattattgc agatactcag 155760 gtagcccacc tctattggaa aaagttcctt gttaattcta ggaaagcaca tttgcctatt 155820 tgagaggtac attttccctt cctattatag aatgatggag ggtttggttt catggagacc 155880 tgctttcaac aaccaacatg taaaaaagct gcctcatggc tagaaagcac tcatgtttct 155940 ccttaaacaa ataacttggc tcaatgaaga aaacatgatt tattgtttta tagcgaaatt 156000 ttaccatctg gagatactat ttctagttat aaccatatta tttgaactgc tcatgtattc 156060 agtgatatcc aaatagtctg tatgttccaa tgggatgtac tgagatacat ttgcacaatt 156120 acacctcatt tgatctgaca atgcaagacg ggctattgga aaatcaggga gggtaagtgt 156180 gtcactccta gcgcttcact gtccactctc atttcttagg ttggaaaaca tgatgaagct 156240 tggatgattc tgaagttaat tcatgacacc aacatgagag cccggggtca gcctgagaag 156300 gtcttcacgg tgagtcttct ccccagagaa tcctcaacac cagggattgg gacatgtttt 156360 ctgtcaaggg aaaaattgta aatattttag actttgcaag tcatacggtc tttgacacag 156420 ctactcagct ctgccattgt agtgcaaaag cagccatagg caatgttaaa ataaatggga 156480 tgactgtgtg tcaataaaac tttatttaca gagacaggca gagggctgtt tttggcccat 156540 aggttgccaa ccttgcttat gcctatctag gactcgaggg ctctgtggtt ggttaaatga 156600 taacaccaac ccaagagatg gggttaaggt ggaaagactg cattttcatc acagacttac 156660 ttactctctg actcccaggt tcagtacccg aggacaagag attcttggtg tagggactgc 156720 cagctgattc tccttgacct gctaccagaa gagagccagg ctgtgcaggg ccatttcctc 156780 cactcggcca ggtcatacct tttcaagtga cactcagccc ccacaaaggt gtatactcat 156840 agcagcccca taacattttc tctaagatgt cagagagaca tcttagagag gtatgcctct 156900 gttaggacca ccatgaggcc tttaacagac cttggaatac atagtaaatg gtaaaattat 156960 ttattgaaaa caaaaaaaaa ccagtatcta ataagtgaga aaacaattat actaaggatc 157020 aaagcaatgt aacaacataa aactaatctc tgggtttcct gatgtattag tccattttca 157080 tgctgctaat aaagacatac ccaagactgg gcaatttata aaggaaaaag gtttaattga 157140 ctcacagttc agcatggctg gagaggactc aggaaacttc tgatcatggc agaaggggaa 157200 gcaaacacgt ccttcttcac atggcggcaa caaggagaaa tgctgagcaa aagggagaaa 157260 atccccttgt aaaaccatca gataccgtca gaactcactg actgtcatga gaagagcagc 157320 ataggggtaa ctgtccccat gattcaatta cctcccactg ggtccctccc acaacacgtg 157380 gggattatgg gaactacaat tcgagatgag atctgggtgg ggacacggcc aaaccatatc 157440 acctgacaac caaagcaaag gataaattta atgaattata tcattattga tataaagcaa 157500 tgtgttcgct tttcaggaaa tgcttttcag gtagtgtatt cacttttcag gaaaggccaa 157560 ttttatcttg ttctaatttg tgaaaagttt attcataggc cttattataa gggatattga 157620 acaatataat ggacattatt ttcagtaaca cttttacata aaggcagaca tttcacacat 157680 ggccatttct taggttaata attaatcaga atttggtaaa ggaaagtagc tatataggaa 157740 accagagaaa ggtaatccaa gaatgtggct tcaacttgat atagcttgac atgaacataa 157800 ggtataaaag aatcatagat atagttccaa aaatatatat ttcattttat tatacatgca 157860 ggcacatggg cacacacata cataacacat accccagttt cacattctag cagcttccca 157920 ttgctctcag gataaagaca gaaaccctcc ctccggacac taaggtcctg ccagtctcct 157980 tcctgcctgc tttatagttc ccctctctct gcagctacat tcatccctca gctcctcaca 158040 ctcaacattt gacttactat acagcactac cacagtgctg ctgttccctc tgcatggaac 158100 attcctccat ctctagttac aaagctgatt ctccatgggg aaagccagta taaattaatt 158160 taaattggtt gcaggaaatg tttcggaagg aacaattgtt taaaatcttt caatatacgc 158220 agtcatataa ttagactaaa aatggccagg cgtggtgact cacacccata atcccagcac 158280 tttgggaggc tgaggtgggc ggattacaag gtcaggagat cgagaccatc ctggccaaca 158340 aggtgaaacc ccatctctgc taaaaacaca aaaatcagct gggtgtgatg gcacctgcct 158400 gtaatcccag ctactcggga agctgaggca ggagaatggc ttgagcccag gaggcggaga 158460 ttgcagtgag ccaagatcgt gcctctgcac tctagcctgg caacagagca cgactccatc 158520 tcaaaaaaaa aaaaaaaaaa atagactaaa aatgttttgt ctacagtagg ttggagggac 158580 atatggtaag acataagtgg gaacctaaat aagatcttca gtggcttata cctaacaaac 158640 atcaattaca gttcttaaaa cacatgaaat cacttctgat taaatagtcc cctgctatct 158700 catttattaa tttcatttta tttaggaaat gaatttttca tactgtaaag ataaactgca 158760 atttggcctc tatccaaatt atcacagatt cttaattagc aaaattttct ctgcagtata 158820 atggagaaat gaagataagt aatggatatc ctcaattaag gcctggaagc ttttgaggac 158880 tctcttcttt tgagtagata tacaatagag gggaaaaatt ttaatttaaa ggatcataag 158940 gctggaagaa tcttgagaca ttatctggat atacaacatc acttaactct tctcagccag 159000 atgaagaaaa accctttatt taccagacct tctgaggagg tgtctctata gggcctcatt 159060 gccctactta cctgcctttt cttccaaaag ggttgagtta ccaagtccac taatggcgat 159120 gtgttaatgt tgttacatga aggttagagg agaataagat ggatatactc caaaagtgat 159180 tgataatttc aaagtcattt atatgtgact ttcaaaaagt tgttacatga ttctgccata 159240 acaaatttat ctttttattt tttaaatcca cggtacagca aagttgcatg cattttccta 159300 aggtctcctt ttcttttttt tttttcctta ttttttgaga cagggtctcg ttctgttgcc 159360 caggctggag tgcagtggtg tgatcacagc tcactgcagc ttcaacctcc ctggctcagg 159420 tgatcctcct gcctcagcct tcctactaac tgggaataca ggcatgtgcc accatatctg 159480 gctgattttt gaattatttg tagataatta acaacaggtc tccttatgtt gtccaggctg 159540 gtctcaaact cctaggctca agtgatcctt ctgcctcagc ctcgcaaagg gctggaattg 159600 cagatgtgag tcaccatgcc tggccctctt aaggtgtcct ctgaagctct tcttagtgtg 159660 cccttacctt aaatattgtt ttcgaagact tcattttcag ctaacttcca ctttaaccct 159720 attcactctc cttagctgat ctcagctatt tcctgcatgt atcctaatgc acaaatctcc 159780 tgagctccac acatgcaggt tgaaggactt tgtgggcatc cttctagatc acataggccc 159840 ttcaaaaatg gcatctttgg gcagtgaggg gtggggaaag atggcatctt taaatctgaa 159900 tttatcacct aagcagtctc caagcctcat cattgcccta aatttcatca gtaggtacca 159960 caagcaccac ctaaacccaa aaactatatt ctttctttta tctttggtac atctaagaaa 160020 ccattatgtt ctattattcc acctcctaaa tatcactcca ctgctgctga tttggtgcag 160080 tcctccttat tcactgccct ctggccttct actatccttc aggaatcgcg aactctttgc 160140 agtttcccgg tgcactattt tccctcctaa ctctggacat ttgtttgcac atggtatttc 160200 tcccacttgg aatatcctcc aagcaggtta catccatggc agatagcgta tttctctata 160260 agcctgcagt tcctgaagcc tctctctcac ttccaggtta agtaggttgt tctgaacagt 160320 atttccttcc catcttttat agatccccat tagagtactt ttttcgtttt gtatggtaat 160380 tgtttggagc cacatggctg cctatgtttg catcccgcat gggtgccaat gaataaatga 160440 acaaacaaat atcgatgagt gaaaaagcgg aagcctccca aaagtagtat gttggattaa 160500 gactatccca tgatgtaaaa agaaaagaaa aaaaaaactt tttaaatcta gagttttaaa 160560 aaacataatt cctacatgga ttattttgag ccgattgcat ttgatttgga attccatatt 160620 taactcagag attatttttg ctaagtacag acatctttgt ttacctggcc agaaagaacc 160680 tactgcctac ttggtataat ttgaaaggtg ctgagggtcc ccaagccacc tctatccaaa 160740 actggcctag gtacctgtac tctgccaata gagttacccc tttaaatgtt ggcaggatgt 160800 ccggaaaaac ctgttcaaac ctctggcagg ggtaatgtgt acacatttcc atgtcctctc 160860 agatttatca aaaacaggta aatcttgatt agcaggagaa ggcgggtcag gcccacaaag 160920 gacccattct tcattttttt ctattttcag gattcattct ctgtgcttct cctatcttgt 160980 ggttctcttc cttagcaaaa agaggctaac tgagtcatag atgctgcttt tcatgctgaa 161040 cctctatcat ttatttccct tatatggctg agaaattcat gttgatgctt gcagtcaaaa 161100 atccttcact taatcttgaa tcatggctac ctgtgggtat ttgctgacta gccactgtgg 161160 gttaggctgt gggagaagga agaaaaggac cggaagtggt gctctgagaa tagtgatggg 161220 aatgctttca gaactatagt taggggtcaa attcatcaaa taagtaggtt tcaaagaaaa 161280 aaatgtgtag actcacttta gcccaagcta aataattctt aatccagaat ctaacttatc 161340 tggatgtttt gtctgctaat gtgtgtctct gtagtaatgg ttcagggagg gcattttggt 161400 ttcacattat tctattacta cttgcccttc ccccatttct ttgtggtttt ccctctcact 161460 cattccttct tcagttcttc caggtgctgt ttggatccat atacttatgg cagtggtctc 161520 atatatgtat gaactgatta ccaaataccg cctacttctt gccggagtca gtttttgctc 161580 ctgtaaaaac aaattataac cctacactca gcaacttcag agaaggtaac ttagcgcttt 161640 ggtctgtttc tctctacagg taaacaaaat aaaaactcct aaacaaatag atgagctgat 161700 tgaaattgag agtgacacag gaacatggta taggaggtgt tttgttcgga tccgcaccga 161760 gctgtacgga gtaagtaaca agtcccatga tgacctgcat gttaatttat tgcatttgag 161820 gttagtcaga agaggggttt actgggcctg ccctatgagc gaggttcttt cctgctgccc 161880 aggacacctg actcctggac tagagccagg taattggatc ccactcgacc agcatttagc 161940 caatacattc catctgtggg aagggaaagt gttaggccag gagtcttggc agtggcaggg 162000 tgccttcaaa tcttgagtta ccttgtccat tgacatccag gaagctgaag aatgaaaatt 162060 ccaattacct ttaactttta cattttaagg tgaaatgaat ccaacccaat gacaactcag 162120 cattttttat aatttggtgg aaggggtcgg ggagtggggt tgactaagta actgcatgag 162180 aaaactaact ctctaatatt tcacagattt ggttgacttt tatgagatgt ttcaactacc 162240 cagtcaggga taatacaata aagcttacaa ttgtttggtt caccctgtcc tttgggtaag 162300 tgatatttaa attcttggca agcaaaatcg ttcaatgtcc acattgtaac tcctagccat 162360 gctgctttct tttctgatac tgcttgatgc tgttaacctg ctaaggaaga aatttttttc 162420 agcttaggtt tgctgagagt tatggttttt ataaaccagt tattttattt gttttgtcat 162480 aaagctaagg tagcaaaacc aattgtatct gcaaaatcca gtaaggccct ggatgatttg 162540 ccccatgact tctctgtcct gtcctccttt gcccagcccc agccatacca gcctctctgc 162600 tcttctttga acattctaga acagttccca tctcgggcct ttgcggtgtt cctctgcctg 162660 agttgctttc ccctccagac atctgcccag ttcatttctt taccctcttc aactctttcc 162720 tcagatgtca ccttctcagt gatgtcatcc tctcttttta aaaaattgca gcccctccac 162780 actccctatc cccttctcag ctctacttta ccccacagca ctttctacct tctaatatgc 162840 aaaacactcc tttattctgt ataaagagta aagctctggc ttccctctct ggaagctcca 162900 tgaggacagg ggtttttaac tgccttgttc aaagctgagt gtggtgccat ccacctatat 162960 tccaagctat tctggaagct gaggtgaagg attgcttgag cccaggagtt ggaagcttaa 163020 gagtgttata atcacacctg ttactagcca ctgccctcca gcctgggcaa catatcaaga 163080 ccccatttct aaaaaaataa taataatttt gtttatgtct tgttcactga tgtatattcc 163140 tggcatgtaa tagcagctca ataaatagtt gttgccaaat gaattaaact gccatatatg 163200 tgttccagga aaagaggaaa tagggagaaa ataacaagat tttatttgtt aatcgctgac 163260 atagtcactt tagaatgtgt gtgtacaact ctattgttgc ctaatttgct gtttagaaaa 163320 gaaaaattag ataatactct acactttcag gtcagcagga actcagtacc ttatagaacc 163380 taacatttat gttgcaagtt tcatctctta acatccctaa atcaaaccag gcaagtgatc 163440 taaggtgacc actctttctt agtgcctgag gaggtggaat acggagaaag aaggaagctg 163500 ggagtcagga gacctagcag cccattctgc tgggtgactt ggttagcttg tgacttggag 163560 acatttctca gttcctcatt tctaaattaa gagggttgac ttagaatatg taaagtaatt 163620 cccacccaag ctctagaaaa tccattaatc tctgaaggaa gggaaggcta aatattaacc 163680 tatattaagt gcttactctg tacctaagca aggggttaaa gacaaagaag acaaagataa 163740 gcaagaagga agtcctgctg tcaaggtgta aagagtaatc atcattatct ggcttgttta 163800 ttttgtttac taattcatta ttgatcatat tttaagcttt atgatttgtt ttactgctat 163860 atccctggaa cctcaatagt gcctagtatt tagtgcgtgt tcggtaaatg tttgctaaat 163920 aaatgacaaa ttagccaaaa gccctccaca tcattgatga tggatggaca ttccaaggta 163980 atgtgccctc ctcattgttg ccatttcaag gtttgtgccc catgtatgtg actagagaac 164040 aatgccataa atgttgtacc cctaagtcat ttcttcctca ataactaccc tcacaccccc 164100 acagacatta ctatgaagaa aaggggtggg aatcctgagg cacagatgtt tttggaatcc 164160 taatgacaat gatgataaaa acgactcata ctgtcttctg agcactttct atgtgctagg 164220 tacttgacat cccatttaaa ctgagcaacc acccaggaag gtggatattc ccatcattcc 164280 aatagtagag atgggcaact gaggcttaga taactggccc aagtttaggc agctagtaag 164340 cgacaagacc aggattcaaa cccagcctgt cctcctgcaa aggctatgcc catactctct 164400 tctttccctg tgtagctaag tgtcactggg aataaactag agcagtggtt ctcaaacttg 164460 aacgtgcatc agaagaatcc ctggaggact cccctgccaa agtttttaat tcatcaggtc 164520 tcactggaac ctgggtttgt gggggatgat gatcctgctg gtccggggac cttatcttat 164580 cttaggaggt actactttag agcagcacag tctaatagag gtatcgtgtg aaccacagat 164640 gtcatttcag attttctggt agccatacac acacacacaa agtaaaagga aatagataaa 164700 attaatttta atactacatt ttgtttcatt cattctctct ctcttttttt tttttttttt 164760 ttgatggagt ctcgctcttt tgccaggctg gagtgcattg gcacgatatc ggctcgctgc 164820 aacctccgac tccctggttc aagctattct tctgcctcag tttcctgagt agctgggact 164880 acaggcgtgc accaccatgc ccggctaatt tttgtatttt tagtagaggt agggtttcac 164940 catgttgacc aggctggtct cgatctcctg accttgtgat ctgcctgcct cggcctccca 165000 aagtgttggg attataggcg tgagccactg tcatttcaac atatgatcag tataaaaatt 165060 attagcaaca atttttacct tctttttttc cgtaagtctt caaaatcctg tggtatttta 165120 tatttatagt atatctcagt ttgaaccagc cacatctcaa ctgcctggta ggcacacgtg 165180 gttagtggct tctgtactga acagtacagc agcatgacac agctgttaga ataacacatt 165240 agaggtcaag aggcagaaag gggaaataac ttggaatctc tgagaataac acgaatgtcc 165300 tcttcccagc ctctaactac cttgggaact ggcattctac ccccaggata gcagtatcac 165360 agcagtgtag gaaccaagaa agattcccca ttagcaaaag aggtcattct ttaggggcta 165420 ctcaccgtgc ttggatcccc aggagtttgt accaccagac taggagagag agtttctcag 165480 agcagactgg aatgatgaga aagatattgg ggccagggat gtcagatgtc ttactccatt 165540 tctgctgcta taacaaaata ccacaacagg taatttgtaa ataataggaa ttttattttt 165600 cacagtcctg aagactggaa gtccaagctc aagctgctgg cagattcagt gtgcagtaat 165660 ggcacagtct ctgcttccaa gatggcagct tgtcgctgag tcctccagag gggacaaata 165720 ctgtgtcctc agtggtagaa gggatggaag ggaaaaagtg agagaggcag caccctcgca 165780 cctcttttta taaggacatt aatctcattc atgaggtctc tgccctcata acttaatcac 165840 ctcctaaagc ccccacctct aatattatta tactggggtt taagttccaa cacataagtt 165900 ttggaaggac acattcagac caggcaccat gtatgggcat aggatcggat gctttctgca 165960 tgtctcttcc caaaggaagg gtttaggaat tacatacctg gggttctcca gctaaccata 166020 ggccatctct caagcttgat ttttgcagat taatgctggg ggagagggac acatggattg 166080 ctgtatgatg aattactcat aatgttatag ggagccagcc attctccggg aactattacc 166140 agtctgaaaa catcagggag ttgctcattg cctctggcat gctagtgcct ttacaaactc 166200 acatttcttt gtctttgcag gtactatgga ttatccgttt ggttccctga tgtcattaaa 166260 cctctgcagt ccgatgaata tgcattgcta accagaaatg tggagagaga taaatatgca 166320 aatttcacta ttaactttac aatggaaaat cagattcata ctggaatgga atacgacaat 166380 ggcaggtcta gaaacttgaa ataatttaat ttgctaccta ttcacaaaaa cttatttctt 166440 cttagctttc cctgcactga aacaggcata gttttttgtt tgtttgtttt agtttgtaca 166500 tttagtcatt ttcatgatga atttcaatac ttcttaatag gctttaaata aaaggtacaa 166560 atgtatccat tacttcactt aaaactattt ttgcagctaa gttccttcat atttattagg 166620 tagtggactt gactggtgtg gaaggaaaat ttagagcaat gttggagagc aacaaaaata 166680 atggaaggta agagaagagt gaagagtatg ggaaaataag acaggctata ggattattta 166740 attcacagaa gaaatctgaa atgtgattat atgagtcttc tattcctgag aaacagagcc 166800 acttttcaga attcatcctt cccaatacgc tgaatccaga attgaggtta aattgcatca 166860 ggaagagttt caattagaga acaggaatca ttccccaaag taaagggctt tgaaagacgg 166920 aagggtctcc tttatgggat ctgaaatact tcttgatgac aagagagaac tgactcaggg 166980 aggtggtcct gggtcattct tttagagatg gaaaagagta gaccaagtac ttgtttatat 167040 ccaagtattt ttgttgcctg aacaaataat tgccagtgta agttcatgat agtttacatc 167100 attcttagga agttgtccca atttcaaaag tgataaaata aaactctaaa ataatcaaag 167160 ttccccaggg caaaaaccag ttcagaaaga tgaaaaagaa ttcagtggga tgcttatgat 167220 ctatgtaatg gaaaagaggg ttactaacta ggaggtaatt gttcaaatta gcaattcaat 167280 gaaggaacta atcatcatcc aagccaagtg ggatcacctt cactctccca cataaaatac 167340 tgacttgaat ctgcacttag catttcacag aaagcttagt gtcaagtcct ctgtgatgcc 167400 agggtgaaaa ctgactttca aaagtattgt tactccatta gaaatttcta atatatgttt 167460 gtgtctctga cagtcatatt tttagtaata tatgttaaag ttctaaactg gatactcagt 167520 cactgcttag aactttttta cattagcaat ataatgatta ctaattttaa tagtattaca 167580 gtttttgtaa aacatatgtt acacatttat tttaaaagat gatagaaaca atttttttgt 167640 ccttcaggaa aatttgaaat accattaacg aaatttttta aaaaatagct tttttattca 167700 gcatcataaa atggcaactc tttatatact gatacggaat aacctccaag gtagactaga 167760 aggagataaa gcaggtgtac tttagacaca gaatgctcct ggaggagcac acaagaaact 167820 gacaacaatg gttattctgg gcagaggaac ttgtggcctg gggatggatg ggaaaagact 167880 gactttttcc attttcaacc tttcgaattg tgcaccatgt gtactctctg aattatccat 167940 tcaaaaaata aaactgattc attccctttt ccaaattcag ttccatgaca gaaaaaattg 168000 ataacttttt aaagatgtgc cattatttag tctcttttgg tgcatttaga tgtttttact 168060 ctgggtccct ggagtgggga ttgaaacgag atcattatta ttatttccag gtaatgtacc 168120 atgggaatga tttctttttt aagctgattg gtctgtgagg ccatgtgcat tttgaaccaa 168180 cacacgtaat tgggtattag ggagagaata aatttgaaag tactcaatag ttagtaaact 168240 gcttttaaat caacctactt acctagaagg atcagtccct ttctgcccct aataagagtg 168300 gtattgacat aacaactcct tcccgaggag aacaagcatt ttcctctcag tgactggcta 168360 agtgtacatt catagtctct taataaagta ccaggacacg ttaatgaata tttatctgtg 168420 tctgctggga gtccttatca atgctgataa ggcctggatc atggactctt ctaagatgtt 168480 attatcatta tgactgttac catttttacc acgtagaatt cccccttata atacccaaaa 168540 atatacctga acaggtttca tcattcttca tgtttccaaa aataagcaga gttctcataa 168600 ttccttttgg ttcaaggact atctgttggc tgtgagaaga aaaattatcc tggaaatgag 168660 cgtcacctat gagtttcttc aaccccaagt cagttattcc tcacataaat gaaacagaat 168720 tctcatgctc tctcttcagg ataattacag gtccctacac tagaatgaag aagacttgga 168780 ttttcagcta gggaaaaata aagttattaa aagatcgggt aggaggacat ccttaagtta 168840 gagccattaa ccccaaatag agaatattca aataaagata tacatgaaga gacgatagga 168900 ttaaaaccta aagaatagcc ccaggtaatc cccaaactat cttcagttgc ataccccaca 168960 aaacttttta ccaacattac aaaaagctaa gggaagttag gagtgtcatc tcttttcctg 169020 gtgaaaaata atattggtaa gcaataagat agccaaaggt cagcctggat gagaaaaagg 169080 ggaaaaagca gagagctaag aaaatcaaga actctgtgtt ctttataatt aagacagtcc 169140 atagtggggc attccatctc taaaactatt tgacttagct ttgaactttg atggacacag 169200 tcattgattg atatgaattt ttctgtgtgt tgggcagatt cataggggtc aagttcaaat 169260 ctgtaacttt caaagactct gtttttaagt cctgcacctt tgaggatgta acttcagtga 169320 acacctactt caagaactgc acatttattg acactgtttt taacaacaca ggtaggtgtg 169380 ctacttagta ctgcctctac ctgaggcctc tccaaagggc ccactgtgat aaccatgtga 169440 taatgtgggc agaggcttat gcgggaagtg gatattaaag aataaatgga acaagttctc 169500 taaatcaggt tggatcaaag gttggcttgt attgtttgtc tttgtcagat ttagtgcaaa 169560 tggagcttgc caaagttcaa aagttggcaa caacaacaac aaaaaaatcc tttgatgaat 169620 aaatttttaa agcaaagacc taacctgtag tttatttgtt ttgataatcc tgggttcaaa 169680 atctggtgtt tgtttaaatg cctcaactct atagccaaca gagatagaca acatgtggct 169740 atgctcagat acatatatat tttggccaaa tccatataat ggggtatttt aaactcccca 169800 gggggaaaaa aaaggaaggc aaacaagcat tgattgaaca cctatatact gttttctact 169860 ctaggtcatt agacattcat tatctcattt agttttcaca gtagctgagg cttaggaaag 169920 ttaagtaatt tgtccacaat tatagaacta gtaataacag atgtttgaac ccagatttat 169980 ttgatttcaa agcctgtgcc ttccactttc ccactcccaa acattggtct gtgtgtccta 170040 taaaatgaat ccagcataac agcgtggcat tggcaggagc atcagcattt gccctgttgc 170100 cagacctgcc ttcttagtgc tcagacaagg gctctgccta gctccaaaga agaatgaggt 170160 caggtgtctt tctgtttttt gttttttgag caatgctatc tacctgtctt ctaacccatt 170220 aaacctcaga agacgaacat tttgtcagat cttgatcagc aaggaagata gtttcaattc 170280 tcacagcctt ttagcagaaa ccaccaccat ctgcaactct tcttggatgg tttcaaattc 170340 aagaaaagat tagcaatgaa tgctggtgac cttcagatga ggctgactct tgttcctcag 170400 tagattcatg cacatagact ttaaattata aattcagcct caggaaagac ttgttccttc 170460 tctgtgccct ctagtctcat aaaatggcaa tgataaggct gactcttgca cttttttttc 170520 cttagagaca gggtcttgct ctgtcatcca ggctggagta cagtggcgca atcacagctc 170580 actgcggcct caaataaaaa attattatta ttattattat tattattatt attattatta 170640 ttatttttgt agagatggtg tctcactatg ttgtccaagc tagtcttgaa ctcctggctc 170700 aagcaatcct cctgcctcat cctcccaaag cactgggatt acaggcatga ggcaccacac 170760 ccagcccatg cacattttga aacaaattgt ttttataatt gtagtatgga cactattagt 170820 actataatga tagtctttat gtaacatgat aattaggaca tagtaatatt gtaaaggagt 170880 aagcaagtaa tataattagc aatggccgac cataggaaat cagaagataa ctggacccct 170940 taatttaatg tattccttta ttcagcaaag gtatctcatc attcacctac tttatgatgt 171000 gaagacccag actagatcct atgctttggc cagaagaccc aaaaagtcaa gctagcatag 171060 gcctgaaagc cctcaaggaa tttacaatac tggtttcctc tccgaatcct ccataggctt 171120 tcttatactg gtgcatgcct ggtaagagct tgatgaattg tctttgttgt tgtttctaca 171180 gattttgagc catataaatt cattgacagt gaatttaaaa actgctcgtt ttttcacaac 171240 aagacgggat gtcagattac ctttgatgat gactatagtg cctactggat ttattttgtc 171300 aactttctgg ggacattggc agtattgcca gggaacattg tgtctgctct gctgatggac 171360 agaattgggc gcttaacaat gctaggtatg tactcagttt catgtcaaat aaaagagctg 171420 cccctgcaat ccagagggac cctgtttccc cctgtactcc catctattag ggatttgcat 171480 ccaataagga gaaatccttt agaccttacg ttgggactaa attatagtga aaattatgtc 171540 taattacatt tcatttgagt ggtttccatg agttaacaga ctgaaaaact atgtgtaaaa 171600 tgtatatttt cccactcaag tagctgagta aaagatgata tgcatttcat actttctgtg 171660 ttcagaggca gttagagcct ttcattcagg tgaatgcacc agttcttgag ctttatggag 171720 cccatcctgc agcttccact tagaattcag ttttcttgag caatcccaag gacccaaggg 171780 ttcttgcttt actaagatga caggaaacat tccagccttt tgtctgcatt gttggcaggt 171840 ggctctatgg tgctttcggg gatcagctgt ttcttccttt ggttcggcac cagtgaatcc 171900 atgatgatag gcatgctgtg tctgtacaat ggattgacca tctcagcctg gaactctctt 171960 gacgtggtca ctgtggaact gtaccccaca gaccggaggt atgttgaaat gggcctctag 172020 taagaggcgc tctaagccct gtgagaccac tgaaaaatta ttttaaaaca acccaaactg 172080 gctgggcacg gtagcttatg cctgtaatcc cagtactttg ggaggccaag gtgggtggat 172140 cacctgtggt caggagttcg agaccagcct ggccaatatg gtgaaacctc gtctctactt 172200 aaaatacaaa aattagccgg gtgtggtgcc aggtgcctgt aatcccagtt actcaggagg 172260 ctgaggcagg agaatcactt gaacccagga ggcagaggtt gtggtgagcc aagatcacac 172320 cactgcactc cagcctgggc aacagagtga gacaccatct caaaaaaaaa aaaaaaaaaa 172380 aaaagccaaa ctgtcacttc cccaggaaag taaaaatcca aaaatatctg gcttcacaaa 172440 gttgtaggtt ccactcatat cttttaaaaa gaaaatatag gggaacatat gtgctagaag 172500 gaattaatag ggcaatgaag ccagtgaagg aatttaagtg tgtccttaac ttagtttgta 172560 ggctaacaat gtgaggaagc tgtaatgaag cagtgaggat tagagtttaa tagcctatct 172620 tggcccacat tttaagtggc taaccagtcc ttagagggtc gtttccttag gtttgcagat 172680 gtgtctgcat gagctctgca ttaagcaacc acaaagaggt atctagaaca tatctcttga 172740 ccttcctggt ttgttggagg aaagccaaac acatttctag ttataaaaaa caattgtgct 172800 gggcctagtg ggacatgcct gtaatcccag caatttggga ggccaaggca ggcagatcac 172860 ctgaggtcag gagtttgaga ccagcctggc caacatggtg aaaccccgtc tctactaaaa 172920 atacaaaaaa aaacctagcc aggcatggcg gcgggtgcct gtaatcccag ctactcagga 172980 ggctaaggca cgagaatggc ttgaacctgg gaggcggagg ttgcagttag ccaagatcat 173040 gccactgcac tccagtctgg acaacaagag tgagactcca tctcaaaaaa aaaaaaaaaa 173100 aaattgctcc caatatggaa acacagagac tcatatcaga gaaagggcta gggattttgg 173160 gtatagcttc agcagcttga atagctaaat taaaataaaa gaagctctgc tttaccattt 173220 gatatgagct aagacaaatg gactttgcct tcatgtacca ctcctgtgct atgttgccat 173280 agaagtcctc tgtgccagag ggtggagtca gtattatctg gactttgaaa tgtaggagag 173340 ttttttccta gaactatgcc aaaacatttg ggatatcaat ttgcaactga tcttgagtgc 173400 ttcctctggg ctcattccct ttttttattg accccaaaga ggtgtattct gtgtgtgtcc 173460 tgtcctgtct ctaacttata tttcttcttc aacaacatct attactgagc ttgcagaacc 173520 ctgtaggtac ccctccatcc atctccctgc agaacatcta acactcaacc ctggatgtct 173580 gcttatcaaa tattctagtt cagaccctca gtgacccacc tattaaatat ttaggctctg 173640 aaaggaacca tacaatcttt gtacggcatc tccttcattc attcaacaaa tgttaattct 173700 ctgtctttgt gaacgaggtc ctgccagagt tggtgcggcc aaaagcaaga ttaagactct 173760 gcagcttcat tgagctttct ttcatcaacc tctcattctg caaacagagc ttagatgtgg 173820 attacactat acgtttaaaa tgaacccctc aattgcttga gcccattgtg ccactgcact 173880 ccagcctgag cagcagagag aacctgtctc aaaaaataaa taataaaata aaataacctc 173940 ctcaatgtta aagcaaggct aggtcatagc ctccttcctt tttcttgtat actataagag 174000 tgtgatactg ttttaggaga atcagctgct tatccctctg ccataaggaa tagaaggatg 174060 agtattcacc taggacaaaa atctggctta aaaaaaaaag accctcataa attgaaattg 174120 gtcaggtcct tcctgtgaga agtacagacc tacggtgttt ctgttgtcag aaaggttaaa 174180 tatcaattga aataaaaaca aatgttgctt atttgtatta attcaggtcc tccatgaagc 174240 agatgtcagg atgggattaa acatgcaaaa atgttattag aggaaatgac tgtgagagaa 174300 gatggagagg gagtgaagta tgggagagct gcaatgcaac tctaagcctg agcgaaggag 174360 ggaggaagga gtatttgggt ggaatctccc agactatcca gtaatctaag gaaggtttgg 174420 caaggctgtt ggagacccag ggatcatgac aacatcagcc tcagaggagt cctgtgtgac 174480 tcaggaagca gcctgcagta atagtcctgt caggtccagt cattgtctgg gagcagcatg 174540 tgggaaaaca cctctgtgca caccagcacg ataacacaaa ggtctgccag gtgcattctc 174600 accgcggtca cgctgttgct ggcagacgct ctccagcact taagtagatg tcatcagata 174660 atcacaggaa gtcatttcct ttggtgatct agaagatgac ttgtgctcca gagcagcgtg 174720 accatggcca tggtaaattc gctagtatga gctaattagt gagtatctga aaatgtttca 174780 ggctaaaacc tcttccacca tttccatgtt tctctgggtt gcattaggag gtctccactg 174840 atctctaact cagctattag aggaaaatcc caaccagttg agacactcta tagcttcatc 174900 tccccatgga ggcaggatag aatacaagaa tacaggaata gaacacaaaa attctggcta 174960 gaggctttac ttcattctct tgtgttgctc taacaaagta cctgagacta agtaatttat 175020 aaagaagaga aattcatttt ctcacagttc tggaggctgg caagttcaag atcaaggcag 175080 gtttggtgtc ttgggtgaga gctactgtct gcttccagat gttgctgagt ccttcagagg 175140 agacgaacac tgtgtcctca catggtggaa ggcagaaggg caggaagggc caaacactgt 175200 gtgaagcctc ttttatgagg gccttaatcc cattcaccag ggagaagccc tcatgaccta 175260 atcatctcct aaaggccccc ctctcttaat actgggattt gtattggttt gttcttgcat 175320 tcctataaag aaatacctga gactgggtaa tttataagaa aagaggttta attggctcat 175380 ggttctgcag actatacagg aagcatagga agcatagtgg catctgcttc tggggaggcc 175440 tcaggaagct tccaatcatg gcagaaggta aagggggagc aggcacatca catgtaaaga 175500 gcaggagcaa gagagctcaa ggggaggtgc cacacacttt caaacaacca tagctcacag 175560 gaactcactc accattgcga ggacagtacc aagggcacag tgctaaacca ttcatgagaa 175620 atctgccccc ataattcaat catctcccac caggccccat ctctgacact ggggattaca 175680 ttctaacatg agatttgggt ggggcacaca tccaatctct attaggatta catttgcatt 175740 tactgtgaaa acactaatga ggacgttgtt cattatagga cacatcttct tctactcaga 175800 gtctaccatg aagactgaac atctcagggg tgggttctag gaatcatttt gtattcctcc 175860 acccatgctt tgagaagctg ttatgtgtcc tcacttcata tgataaaaat tgtgtaattc 175920 ttcacatttc cttctctgtt gtgcaatcag gaagcagaga gcctgtgtct ctgctaaatc 175980 tgcatagtcc agatacttgt ttcactcttc cttatcttga tttgccccct cttatgccca 176040 ttttattaat tttgtgtctt ttataagtta tcctacctag atcgtttgat aaagaggcag 176100 gaaattttgg aattaaatct agatgtactt actgaatagc acttttctgt gccaaaaaaa 176160 atgaaacaac catttttcca ttctgtaaat gccatcaaca tcctcctttc ttcaaaacct 176220 cagtatcaaa aatgagcata acaattgcag tcttaaacag atgcatggtg acattctagc 176280 tttttcccca gattagagag cacttacaca ctgactggga catgatgatt taagggacac 176340 aaaaagaggt gggaggggac tgaacattcc aaccctctgg acatatggtt ggtttttctg 176400 gagaccagcc caatccagaa gctatctagg atcccctcag ccaagagtca tctcattagc 176460 ctacaaaaga cactcttttg gtggagcagg gggaaggatg gggtctccct ctgttgccca 176520 gagctacaat ctgtaaccag agctggaatg cagtgattca atcatagctc actacagcct 176580 caaactcctg cgctcaagtg atcctccccc atcagcttac aaagtagctg gaactacaga 176640 tgtgtatcac catgccaggc taatttattt tatttttttt tattttaaga gatggggtct 176700 cactgtgttg cccaggctgg tctcaaactc ctggcctcaa gcaattcttc tgccccagcc 176760 tctgaagtag ctggtattat aggtatgacc cattgtgcca ggctccaaaa gatactctta 176820 tccaagggtt ttaggagctc aatgccggga actggggatg aagaccaaat acttattctt 176880 tacttatacc ttatatttaa ggctggagat tattactaaa gtgcaattta ttatccagag 176940 gatttagcta tgcaaaaggt gggaaactgt acccctggac agcaaatcaa aaacaaatct 177000 acattcccta aaaccttagc tgctagttat aagcatgacc tcaaaaccag aaaccatcag 177060 ggagaaggtt aaaagaattc tctgggtaaa acaaaatgtg aattccacac agcaaaaaat 177120 accataggca catataaatg ataaattgag ggcaggaggg ggttgaaaca gaaatgaatt 177180 aataacctga atatataaat tggaaattca taaagaaatg tcaacaaaca cttagcatat 177240 aaaaatgctc agcttcacta atagtaaaaa ttaattacaa attaaagcaa ttatgacatt 177300 tagctttctc ctattattca gagaagacaa tatttaaaag atagataaga tccagtgttc 177360 tctggggcaa gtgaggggtt actgctgagt ctgttgtggg aattctgaaa acagatacca 177420 catttgtaga gggggatgat ctacagtctt aagtattttt tcccaatagg aataaactat 177480 tgattcctct ggttgctttt caggtttttt ctttgcctgc catggatttc tttgggttta 177540 ctatgtttgg ggtttgctca gcttcttgaa tctgtaggtt tacagctttt gccaaattta 177600 tgaggttttc agccattatt tattttgtca aaaaacaaaa ttacaacaaa tttagtgtaa 177660 agagctagtt gacttttatc tgctattcta gaagcaggca acacctcact ctataaaatg 177720 gggtgagtgc tctaatgagc tgagcagaga aagttggctt cataggcaga aaagctctaa 177780 agagagcaga tacagagaac aaaaagcaga tagccctttc aaagtccctt tctgtaaagg 177840 gctaaaacag aggggacttc cttattatgc tcatttgggt tggccggaat cacctgtttt 177900 ttggaaaact ggctcttttc aaagttcaat ttgattaggt ggcacttagc ctgagtgact 177960 ccattctgct taggactggt ctgctgtggc ctaggtgcag gagactagcc caaaacaatg 178020 gcctcccgta aaatttaact atttgaatac tttttcagtg ctgccctttt tctcttctgc 178080 ctgaactcca gcaacataaa tattagatct tttgttatag ttccgcaggt ccctgattta 178140 gtttgttttc tctgttcttc actttagata gtttccattg ttctgtcttc cagttcactg 178200 gttctttcct ctgtctcctc cattctgctg ttgagcccaa ccactaagct ttttattttg 178260 gttatcatat ttttcagttc taaaattttc atttagttct tctttatttc ttctacttct 178320 ttgctagggc tttctatttt ttccattttt gccgtgatgc tttctaaatt tcatgtgttt 178380 caagtgtgtt catagttgct cattgaagcc ttattatggc tgctttaaaa tattcatcag 178440 ataattctaa catctctgtc atatcagtgt tgccatctat tgattgtctt ttttcattct 178500 gtttgaaatc ttcctgttta tttgtatgac aaacagtttc tgattggaag cagtcattta 178560 catattatgt tatgagaatt tggatcttat ttaagccttt tgcttgaatt ggctttctgt 178620 gactcctctc tggcaagcga aagggaggca ttgccttatt attaccaggt ggacgtagaa 178680 gtccaggtac tccacttaag ctccagtgac acccaagagt gggcattctc attactgctg 178740 ggagtccaca ctcccttcct gatcatggat accatgggag ggagggaagg aggagctcat 178800 taccacccag cagggatgaa agtcccagct ccttacttac ccttctctga tggcatccca 178860 atggtagtgt tgacaggtct tcttagtttc acaagggtgg aagtctaggc taccaccaga 178920 cctttgctga tgtagctggc agttgagcta caattttttc ctggtgtttg gtctgaggag 178980 agaggttatt atctaaaagt tttctatctt gctagactgc cgttttcctg gtccttgggc 179040 tagagagagc aggctttggt tgtggctttt ctttttctgt gcctattggc atttctgggt 179100 tggcaacttg ttcgattcca agtctgagat ctatggagca gaaggcaaac gcagggaact 179160 ccccatcccc aagctcccta gcagttctgc ctttttcctc tttcagtctt gttctgtttg 179220 ttttatatat aatgtgcagg atattttgtt gtacttatca agaagaataa gaaaagtata 179280 tctattccat ctcagtgtga attttaatac tgtttgttta tttactcaac aactattttt 179340 ctgagcacct atgatacaac cgtgagtaaa tcagttgtgg tctttattct cataagcaca 179400 agcaaacttc agttgtaata tagtggtcag ggttattaca tgggaagtgc atgatgctca 179460 gagcatcaga gagggtttct tgaaagaagt accatttaat tgagatctag taggtgataa 179520 ccagtcaaaa agatggaaag aacatttcag ttagagcaaa taccagctaa caagctctgg 179580 aagtaaaaga gttactcaaa gaacaagtca tgaagactct ttcacattgc cttattgtgt 179640 ggggacgggg agactttgag ctgtcttaca gagaatgaca agatcattct tggcctttag 179700 agagctccct ctaatggcca gaaagaaggc agggaggctg gttaagacac ttaagttgta 179760 ggttggaggc cgggcacggt ggctcacgtc tgtaatccca gcactttggg aggccaaggc 179820 aggcggatta cgaggtcagg agatcgagac catcctggct aacaaggtga aaccccgtct 179880 ctactaaaaa tacaaaaaat tagccaggca tcgtggctgg cgcctgtagt cccagccact 179940 cgggaggctg aggcaggaga atggcgtgaa cccgggaggc ggagcttgca gtgagccgag 180000 atcgcaccac tgtgctccag cctgggcgac agagcgaaac tccatctcaa aaaaaaaaaa 180060 aaaaaaaaaa aaaacagaaa gaaagaaaga aaaatgtagg ttggaaaggg gaaaagtcaa 180120 cactcaacat gttcacagat atttagggat acattcaaca aagcttggtg cttgattaga 180180 tgttaggggg aagaaaagag gttgtcaaag aaagatgatt ccagtttctg cgtgtacagt 180240 tgagtgaatg gagatggcag gttttgttta tgttttttaa ttttaaaaac cacctgcagg 180300 agcacaatgg cattttgagg ctgggacaca ggagggacag tgggtctggg ctgtggtcaa 180360 gaaggtaata agttccattt tggacatgtt gagtttttac atcaacctgg ggctgtccga 180420 ttggcatttg aatatttttg tttggatgcc agaaagatac ctgagctggg gatagagagg 180480 tcattcacta tttcattcaa aaaaaaaaaa tgattatgga atgcatatcc tgtgccagga 180540 actattttag tcattgggag acagcagtga acaaaacaga cagaaagctt tgccttgata 180600 gagcttatat tctataatag tttgaggaag acagtccaac aaacagataa gtaaagtaga 180660 tgtagtatat tggatggtgc taagtgctgt agagaaaaat aaagctacgt ggagcataga 180720 gaatgctggg gtaaggagag ttacaactgt aaatagaatg gtctaggggg atgtgactgt 180780 aaagctgaca tttgagcaaa gacccaggca gacatctgga gagagtgtat tcacagcaga 180840 ggcaacagca ggtgcaaagg tcctgaggtg ggagtgtgag agaccagttg gaagagaggc 180900 aaagcttgtc tggctggagc agaatgacca aggtagaggg taagaagcca tggggtcaga 180960 gtggacaata aaagggcttt ggtcttcttg ctgagtaaaa tgaaatccaa tgtggggttt 181020 gggggcagat gagtgacatg aactgactta tgaagtccac agcaagaaat gtggcaagag 181080 gtaatggtgc caatggaggt ggtgagaaag tggttagatt ctgaaaatat gtctaagaga 181140 gagacctgga atagttcaag tcaaaggaaa cagaaaacaa aggcctccat agaaataaca 181200 tgtctgagag gcaagagttg gagtctgggg gtaaatccat caacgtaaga aaatccagag 181260 gtaacacaga tctggactaa gaaataacag acaccagagt taggatacca ggccaggaga 181320 caagagttca gtgagctaga aactcaggct ggcagagtgg aatacaaaaa aactagcctg 181380 gaagcaagtg gtcaaagtat taagatgtct tgatcttcaa aaatggtgta tgacattgag 181440 agaaggcttt gcaagttaga cttccctata acctctgtgc ttaggtcagt agggcagtag 181500 tagtttgcag ctggttctca atgacattca ttggactgac ttgcccaggg ctagaactca 181560 cctcaggtag aggcaagata tcagaactgt ctgcagtgga agttgaaggc acctctctgc 181620 tcatgcactc accttggtca gtcacctcta tcttgatcct aaaccttctt cccaactctg 181680 tagtaatagc ctgagattct tgccaaggcc agcttcccag tgctgtggta cgtctgccac 181740 aatgattgag atgcagtggt cgatgcagtg cccttctccc caaggctctt aattcttagg 181800 cgtagacttg aagaagccag gaatacttgt acttctgcag ggatgagtga ggtgaagcgg 181860 gaagacggaa aagcaaatgt tgcattcagg aatttgtcct tgattgtgag gctggaaaga 181920 caagattgct ttgagaagca ttatgaggaa agaaatgaag agcagggcaa aatgatagac 181980 ctttgaagtt tgagccatta ttgaatatac aagtcattgt atagtctttt aacagtttct 182040 acatcagccc ctcacctgtg tcaaagataa gcagagagcc tattatatgt aatgtagatc 182100 acaaacaatt gatgtggcat gagattcttc taatagacat gaaatatgaa tgtgacatga 182160 gccagacata gaatggattt tccagcttgc cgtaaatctg aaaggagccc caaccatcta 182220 acatactggt gtgggggagc ttccttttcc ctcatttctc cgtccccagt ccctgccacc 182280 agcaaaatta ctttcaaccc tctttgcggt agaactgaga acaccaacag aagatgcatt 182340 tcattttttg ggtttagtct gtgctggtca gcttccctca ataccctaac tgggaaaaat 182400 aagtcaattt tccctcatct cttgaagtct tcaaaatgaa agtatatgta atgacaggtt 182460 gaaatggatt ttttccatcc atcagggtcc ctacttttct ctccctgctt tgtgaaagtt 182520 gtaaatctct caaaagtcat aaatgtagct cccgcggtgt aggcctgttc cagctcattc 182580 cagttctagg ttttggcctt tatgtttctc tgcgtccagg tgttaacttt ctgctgccac 182640 tatgtggctg tggccaagtc ccctgctcac atttcctgtg tgcttatggc tcaggggcca 182700 cttttttttt ttgggggggg ggacagggtc tcactctgtc acccaggctg gagtgcagtg 182760 acgcaatctc cactcactgc aatctctgct tcccagattc aagcaattct cctgcctcgg 182820 cctcctgagt aactgggatt ataggtgcct tccaccactc ccagctaatt tttgtatttt 182880 taatagagac ggggtttcac cgtgttggcc aggctggtct cgaactcctg acctcaagtg 182940 atctgcctgc ctcggcctcc caacgtgctg ggattacaga catgagccag cgtgcccagc 183000 cattaggggc catctttacc cctcctccgt ggcgctcggg gccaggagcg cacaccttgt 183060 gagatgcagc ccacgctctc agggccgtcc tgcaactctg cacagactcc cagggccagg 183120 caagcctgtc tcatggtgtc gtttggccgc atgttcctgc ctttctctgc ctaccttctt 183180 cccttttctt tcccccttat gcccccttat gcatgcacag tttcagacat gaggtcagaa 183240 aggatttgag gatctgattg ttcacacctg ccgatccctg agagtcctgg gcagctgctg 183300 ccatcaccca gtgcagtggg attggtctct tccttgatga gctaaaatca aaacattttc 183360 ccagaattaa gcaacccaag cccttccctt aggactgtcc ttgttttgac agagttctct 183420 tgtttcctcc ccattcttca gttccgaagg tctgtcatct tggatctctt actgaccagc 183480 tgtgcgacct cagggacacc actcagccta actgggcctg gattgtttga gatttaaatg 183540 aggagtatga aataaatagc atctcagggc ctttctagct ctagaattct cttcactgtc 183600 gtcatctggt attctgcaat ggcaatttca ttcattcttt cttgaataca ttctacgtct 183660 aatataaaga taaatatctc acttatttgt gaagattttg gtacagagga atctttcagc 183720 tatcgcgact aaccaaccta tgtaatccac tccatccctg gccctctgct ctgcttgatt 183780 tttccttata tgccttatca ttatattata tatgtttatg ctttggctat ccagcaaaag 183840 tcagattcat tttgtctctg gtagttagaa gtagagggtg aagggggtgg ggaatatatg 183900 cttcctgctc ccacaacctt gccatcaggc aatatattac aatggaagca aatcctgcca 183960 agtgtactag cacaaaaaca gtcacaaagt ttgtttttga gctccaagga agtccatctc 184020 gctgtccttg ggtactgagc aagcccttgc atgaaagggc catgtgcttg atatgctgac 184080 agcagggcca ccagacttgt gggccagcta aacaagggaa tgccccttta attgtggggt 184140 gcaggataag ggggctctag aatctggagt ctagaccagg agtaccattt caccaacttg 184200 aggctgcagg gcttggagga cgcttgtatg ttcattaggc taagggcaga acattctttc 184260 caatcattgc ttatacagaa cagcactcac atcatcagtg aaaaaatcac ctccgtggtc 184320 attaacaaac cttctcatgc agatttctaa aatgatcttt aaagcactaa gtaaccatct 184380 ctggcctgtt gattcttgat taatgtgctt taaattatat gggtatgcat tagagacaat 184440 ttatctatca atcatattgt ttatactgaa gtacttaaag gaaattatca cccagacctt 184500 atgatgagag gatgttaggt tgcatgtgta cttgtttaac tcactagaat gttagctcct 184560 tgagggcaag acattgtctt ccttatttac tgccataact ctaggtgaat gaatgaatga 184620 atgaatgaaa tatgcatttg agaattaatt tggctatgga tgatagtttt cctccccgta 184680 tggggtccag cctcccagct ttccagagtg agcatttggg ccagtgtaag ggagggagca 184740 ctggtcttca gtcctctctg ctgtcctttg ggtccctttc cacttcttcc atcacagcct 184800 gagacctcct gatccttcag ctgtcagctc cccgttcctc ataaagcatt gcctattaga 184860 gatccacctc cccctctccc tctacttcat caacaaagtg gaggaaagca ctgatctagg 184920 ggtcactgaa ttcaggtgca gtcactgcca gaaaaatagc tggataattt ggaaaaggcc 184980 tttcaccctt ctgagccttg gtttcctcat ctgtgtggtg gagggtgggt cgggcatgat 185040 gctggaaatt ttaaatgatc tcaacttatt taattctcat cccaatcctg taagagtagg 185100 tattcttacc ccatgttcag gtgaggaaaa tgcggcacag agaggggaag taacttgtcc 185160 tggatcactt agccttaaac ccagatgtct gactcagtgg tgctgctccc agcacagaca 185220 tatctacctc ccctcccagt aggtgtggga atctattttt aaacagctca cttggctgtt 185280 gggatgcaca gccatgttca tgaccttggc cacagatgaa ctccaagggg tgctcagtca 185340 ttccatcatt atttaccctg gcccaggccc tcactcccta gctaggttaa tcagataaac 185400 aagacataaa ggctcccgtt attaccttcc cagaatattt gatttcaaca ggaataagca 185460 gtcaaaagcc ccttatggtg gtggagatgg gagggagtta aagggctact tgggatccag 185520 ggattcctgt cagctcacaa cttgtaggtg agtccttctc cacttgagtg taaggaaggc 185580 cctgctcgaa ggggtgcagg gtgccatctt gccctcatta aggaacatgg ccaggcgcac 185640 acacacacac acacacacac acacacataa tagatactag ggtaagacag atttttatct 185700 ttctttgaag attagaaaac aattgcattc agtcttttct gacttaatat gaaattcatt 185760 ttttctctct gaaaaaaaaa atatgatagt ctggtatcaa aaaaacaaat atgacccaac 185820 taatttccat taaaatgtct attacaaaaa tcgacttcca acatatttgg aagatctcag 185880 gaaatctgtt agctcagtct ttcatttaat aagtaacata ggtatctgta ccggtcctga 185940 gaagaggtgg tgctgagtga ctacaaaggc tacccagctc cactccccca gaggtgacct 186000 ggacctaaac aaggctaatt ctggacaagg agatgtgcaa gattggtata aaccagccct 186060 tggaggggag tgggaagggt agcgtggagg gaatcaagtt gaggcaactg agtaacagtt 186120 ggaagagaag gaaacaattc aggttaggtg agaagctgcc tctggtgaaa tgaattaagc 186180 tctgtgctgt gagaagcaga ataaacaagg gagtttaata agagcctaag caggaaggga 186240 gaagaaaaaa gggatgggaa gcctttgggt tggatccctc tctctctcct ctccagtgtg 186300 caaagcatac tttgctggcc tcagtatccc ctgcatcaca tcataaacac tttcccttgg 186360 atgttttatg ctgcttgata ttccctaatg aacacacaca aacgaagcag gtcatatatg 186420 tgaaggtgtt aattgcagaa tcacttgtta ccccaaattt ggaaagctcc cattagccaa 186480 tggcatggtt acaagaataa tggttccatg ctaaataaat catttcattt taaacaaaac 186540 atcaacatga gaaaacagta atatgaatga aaaaaaagca gaaagcaaaa ttgtctaaat 186600 cctctgattt cctgtgattt atatgactgg aagtacacta aagggaattc agaaatgaaa 186660 accatttgtc agaaaggtgg agtggatgtt ttgcttttat aatttccttt gtatatgaaa 186720 cccacatcaa ttcaaagaat gcagacatcc ctgggaagac agcaggattc tcaaccctgg 186780 atacaccttc atgtcacgtg gagcttctaa aaaatactaa tgcttgggcc ccaataaagc 186840 aataaattca gaatctctgt gcatgtggcc ctaggcatca gtattgaaca catcactaag 186900 gtttgaaagc tcctccaggt gattctgatg cacccaagct taagcatcat tagttcaggg 186960 catggttata cgtgcacttt ggttcaatca gattgctatc ctaatcctag gttgaaaaca 187020 cctgcttggg agtaggaaat ttatttaggg gctggaggga ggtaagccgt ttagccctca 187080 agaagccttt aggggtgtgt ctaaggcaaa cctgagtggg taccatgagg gaagttaggg 187140 agatgtgaga gtacggggag aggcagagaa cacccctatc cacatgccat ccttctcctg 187200 gcaagctcct actattcctt tcagcacact cctagtcttg tccagccccc ctgagaagct 187260 ttccttcaac tgccactacc ctcagctcga tatcactttc tatgctcctg gctgcataat 187320 actttgtggg tatcttcaac attgcactca gcatgtatgt taccatgatc tcgtctcacc 187380 catcttagtg caaatggagg tttcatctta ttaattttgg ggtctctgga gcttagtgca 187440 gtgtttgaca cttagcagat atttaatgaa tgattatatg aatatgaaaa aaacgcatag 187500 aatatgtaaa ttaggagtgt attaaggaca aatctgcgta ttagacaaat atttaagggt 187560 ttcttgtact tttagcattg taatgtgata gcttagtagg tattcagtgt tacaaaagtt 187620 cttttaggct aacctgatga cctagccatc atttttaata ttttttctct aagataaatg 187680 aaattcattg tgtatttcaa ataaccaact tccagatgaa tctttagatc acaatgtcct 187740 cctgatttaa ggactgccta tacttttcaa aatgccccat gctgagtctt gagtatccct 187800 cttgtaactt ctaccaatta tttaactgag ttgtccagaa aaagcttatt tccaaaaatt 187860 tattctagac aaaaagaacc acccccccca acacacacac acatacacac acacactttt 187920 accctttaac aggtaaattc tatgagctaa gtctggatct acaccatttc aggccttttt 187980 aaaatgtggc acttaggcca ggtgctgtgg ctcatgcctg taaccccaac actttgggag 188040 gccgaggtgg gagaatcact tgagcccagg ggttcaaaac cagcctggga aacatagtgg 188100 gtaccccatc tctacaaaat ttaaaaaatt agctgagctt gctggcacac atttgtggtc 188160 ctagctactc gagaggctga ggaggaggat cacttgggcc tggaaggtcg aggctgcagt 188220 gagctatgat tacaccactg cactccagcc tgggcaacag agcaaggccc tgcctcaaaa 188280 atgtagcact taattgttct tttctaataa tcttatcagt tggtggggta gatattcagc 188340 cagtgtttaa acaatggcta atagtcacat ggtgcctacc acatgccagg cactgcccta 188400 agtgccacca tttttcagtg tccttttctt cttcctagga gcctcaaaaa aaacccatat 188460 ctggccctgc actaaagaca gaaaaatcca caaagaggct ggatgtgagg acaaaattat 188520 cctcatttta tccagaatgg agccagtagt tttcttttcc ccaccaccaa ctaaaacata 188580 aaatgggtca gatgcagtgg ctcatgccgg taatcccagc actttgggag gccgaggcgg 188640 gcagatcatg aggtcaggag ttcgagacca acctggccaa catggtgaaa ccccgtctct 188700 actaaaaaca caaaaattag ctgggcatga tggtgcgcac ctgtaatccc agctacttgg 188760 gaggctgagg caagagaatc atttgaacct gggaggcgga ggttgcagtg agccgagatc 188820 gtgccactgc actccagcct gggcaataga gtgagactcc atctcaaaaa gaaagaaaga 188880 aaaaaaaaac agcatgaaac aatataaata aactagaacc caataaatcg tgaaggcccc 188940 agagagggag ccaccatcca gctgcagagg tggccacatc cccaccaggt gagtctaagt 189000 gctgctgcaa accagctcca tataccagca catgtgtctc agaccaagga agtttatgat 189060 gcagggagga gagccacctt aatggaatga gcaacaaggc agctgcagtc acagggtact 189120 tttgggggaa ataatttaca tttaaaaatt ttaaataagt agattgagta caaattgaga 189180 gcaaaggctt ctggctgaag agatgagaag caaagcatgg agcaatagaa tgacactaga 189240 tcatttcatt ctttcccttc agtgcttgtt gtttaagaga aggcatgaaa acatatttga 189300 tctgaaaaat aatgcattaa ctcattctac agctgagaag tcggcaagtc agggttctgc 189360 aagctctgag ctccccaccc tcaaaggact ttatgagcaa aggtgactcg tactttgggt 189420 gtctgcagaa gcaatacagt ttcctttaag taagaacaga gagtaaatga actaaaagcc 189480 tatttatttt ataaacagat aaccccaagg gagaatcact ttgtgaatgg tttttctaat 189540 acagctgtgt ctctgatgca ttgaaaaatc atgcccagct gggcgtggtg gctcacgcct 189600 gtaatcccag ccctttggga ggccgaagca ggcagattaa ttgggcccag cagttcaaga 189660 acaccctggg caacatggtg aaacctgcat ctctacaaaa aaaaaaacac aaaaaattgc 189720 ccgtgtgcgg cagtgcaagc ttttagtccc agctaccaaa aggctgaggt gggaaaatca 189780 tctgagccca ggaagtcgag gctgcagtga gccatgatcg cgccactgca ctccagcctg 189840 ggccacagag cgagactcca tctcaaaaaa aaagaaaaaa agaaaagaaa aattacacgc 189900 ttcagctgca aatatttgaa agaagaattt acttttccaa ggaaagggta gtcacgtttt 189960 cttgaatgcc gacactaatt tacggaggct gtgtgctttg catccacacc agattttcat 190020 ctaaagaact gcctttgctt ggtaaatgaa aatggagatt cttaagaagg atttgatagt 190080 ctttttcaaa accctcagat accagatgct cctaacgcat ctgcctttag tctgcccagg 190140 aatctcagga agtcatccat tcctcgagta cagtttcagt gatacaagga gatagcagcc 190200 cacattttat acgctattct gagttatacc agaggttcgt cgtttgggaa aatacggtta 190260 ttttaaccta gatgcaaatc ctatctgggt tactctgcag tttcagcatt gagagacttc 190320 ttcatgacag aaaagtggtt tcctttttct tatcagcaac atacactcag gccattttcc 190380 ccggaagaat ccagcttatc ttactactac aacatatatc tggtcattta tgacactaga 190440 atctagaaac taaaatgtta gtttaacaaa gtgaaaagcc aggcacggtg gctcatacct 190500 gtaatcccag cactttggga ggctgaggtg ggaggattgc ttgagcccag gagttcaaca 190560 ctagcctggg caacatagca atagcctcgt ctcgtcttcc tttttttttt ttttttttta 190620 agtgaaagaa gtcagtcttt aaaaagctac atactgtgag gtttcaactc tgtgacatcc 190680 tggaaaaggc agaactatgg aaacagtaaa aagattggtg attgttcagg gttgtgggga 190740 gagaggaatg aatagatgga gcatgggact tttagggcag taaagctatt ctacatgata 190800 ctgtaatgat ggatacatgt cattatacat gtgtcaaaac ccacagaatg tacaacacca 190860 atgtaatgta attgaacttt ggctaataac aacgtgtcaa tattggctca tcagttgtaa 190920 caaatgtacc ataccaaagc aagaagttac tagtggaaac tgagggtata agagaactct 190980 actttttgtt cacttttttt gttgtaaacc taaagctgct caaaaacatc tattcatatt 191040 tctaaaaaac agcaaaaaga cagtttagcc tcatggcata tccatggagg tgggggaggt 191100 ggggacgggg tcattctagt ataattaagt cttctttggt acacaacata taggctcaag 191160 caatttcatt tgaaagtagt tatcagtata ttaaatctcc ttatgttttt agtggttgct 191220 gccattacta gtctcttgct ttaaaaaaag aagaggaaga atatgaaatt agcttaccct 191280 cttctcaaga agtttctttc agtcagagat tctgtcatac tcttcccaca ttccaaacag 191340 ttggcaactt tgcctcactt caggagcctg catactctac tttctaggtg tcttagccaa 191400 aaagaaaatc cgtgcttact gccctttatt aggtgtgatg taacccacaa aatcactggc 191460 tgtgcactaa ctcacagact ggatttgtgc agctcaggga ggagtgttta ttcctttccc 191520 atggtgcaga aaggaggatg gttggataat aaccacaata aatatctcgg ttatgaagag 191580 tccacacgtt atggaaacaa ctatcattag gtacagtaca agtagcgatg agtagttatg 191640 acttgtcaaa gaaaaaccca cacttacaaa tttacaaatc aaacaaagaa agatgtgtgc 191700 taaatgctgc attttgcccc ttataagatg catttccctc ttagggggaa aaaaaaaaca 191760 aggtatccaa aaattgccct gtattcgatg tgcctttaac tcagtgacag ccaaaaaaga 191820 gtaaactggc ataaagtcag aaggaaattc atactctctc atggtgtcag tggtgtatgc 191880 aggctcatga acaaaattaa gtttcagctg ggaatggtgg ctcacacctg taatcccagc 191940 actttgggag gccaaggcag gaggatcact tgagtccagg agtttgagac catcctgggc 192000 aatatgatga aaccccatct ctacaaaaaa atacaataat tagctgggca tggtggtgca 192060 cgcctgtagt cccaactgtt caggaggctg aggtgggagg atcacttgag cccagtagat 192120 cgaggctgca gtgagccaag attccaccac tgcactccag cctgggtgac atgagcgaga 192180 ccctatcatc tctgaaaaaa aaaaaaaaaa attaagtttt ctgcctagaa gatcacttgt 192240 tcatgtgtac taaagataat aatttttttc ttttttaaaa aatgtcttaa gaagtgggat 192300 gtgtcttact tatatacctg cgtatcttaa agagagggaa caagatcttc ctaggcccac 192360 aattgattct cttctgtcat attttcttgg acttgatttc ccaaatgtta cattggctca 192420 ctggtgtgct ccccagctca gtattcggtt tctgcctgct accaaaaata attggcaaga 192480 tagcagagta tttcacctcc atagtatctg aaagatgagg gatataacct caccctccct 192540 aattttctga gaaaattccg tgggctcttc ttatttaaga cctagcatgt taattaataa 192600 aagaaaatag aaagctgata agcaacttca gcaaagtctc aggatacaaa atcaacgtgc 192660 aaaaatcaca agcattcctt tacatcaaca atagacaagt gagagccaac tcatgagtga 192720 actcccattc acaattgcta caaagacagt aaaatacctg gaaatacaat ttacaagggc 192780 tgtgaaggaa ctcttcaagg agaactacaa accactgccc aaggaaataa gagaggatgt 192840 aaacaaatgg aaaaacattc catattcatg gataggaaga atcaatatag tgaaagtggc 192900 catactgccc aaagtaattt atagattcaa tgctattccc atccaactac cattgacatt 192960 cttcacagaa ttagaaaaag ctactttaaa tttcatatgg aaccaaggaa gaccccatat 193020 agccaagaca atcctaagca aaaagaacaa agctagaggc atcatgttac ctgacttcaa 193080 actatactac aaggctacag taaccaaaac agcatggtac tggtaccaaa acagacatat 193140 agaccaatgg cacagaacag agacctccga aataacacta cacatgtaca accatctgat 193200 cttcaacaaa cctcacagaa acaagagatg gggaaaagat ctcctattca aaaaaaggtg 193260 ctgtgaaaac tggctagcca tattcagaaa actgaaactg gaccccttcc ttacacttta 193320 tgcaaaatta actcaagatg gattaaagac ttaaatgtaa agcccaaaac cataaaaacc 193380 ctagaagaaa acctaggcaa taccactcag gacataggca tgggcaaata cttcatgatg 193440 aaaacaccaa aagcaatttc aacaaaagcc aaaattcaca aatgggatgt aattaaacta 193500 aagatcttca gcacagcaaa agaaactgtc atcagagtaa acaggcaacc tacagaatgg 193560 gagaacattt ttgcaatcta cccatctgac aaaggtctaa tatctagaat ttacaaggaa 193620 tgtaaacaaa tttaccagaa aaaaataaac aacttcatca acaagggggc aaaggatatg 193680 aacagatact tctcaaaaga agacatttac atggccaaca aacatatgaa gaaaagctca 193740 acatcactga tcatagagaa atgaaaatca aaactgcaat gagatactat ctcatgccag 193800 tcagaatggc tattattaaa aagtcaagaa acaatagatg ctagcgaggc tgtggagaaa 193860 taggaacact tttacactgt tggtgtgaat gtaaattagt tcaaccactg tggaagacag 193920 tacagcgatt cctcaaggat ctagaaccag aaataccatt tgacccagca atcccattac 193980 tgggtatgta cccaaaggaa tataaatcat tctactataa agacacatac acatgaatgt 194040 ttattgctgc actatttaca atagcaaaga cttggaacca acccaaatgc ctatcaatga 194100 tagactggat aaagcaaatg tggtacatgt acaccatgga atactatgca gccataaaaa 194160 aggaatgaga taatgtcctt tgcagggaca tggatgaagc tagaagccat tatcctcagc 194220 aaactaacac aggaacagaa aaccaaacac cgattgttct cactcataaa tgagagttga 194280 acaatgacaa cacatggaca tagggagggg aacaacacac accagggcct gtttagggga 194340 tggggagtga ggggagggaa cttagaggat gggtcaatag gtgcagcaaa ccaccatggc 194400 acatgtatac ctatgtaaca aacctgcacg ttctgcacat gtatctcgga actgaaagta 194460 aaatacaaaa taaataaaaa ataaaacact tagcatgaac ataattaatt aaagaaaata 194520 gggagtaaca aatctaagta aatatattgt tgcacttaac aacagttttt tttctcttta 194580 cttatctgat agagacaggg tcttgctatg ttgcccaggc tggtctcaaa ctcctaggtt 194640 caagaaatcc tccaacctca gcctcccaaa gtgctgggat tacaggtgta agacaccctg 194700 ctcagcctgc catacatttt aactttggaa tgaaatttaa atttccattt aaacatagat 194760 gtttctgaaa ttatgggaat ttacaaaaca aaataacaag tcactcttgt aaagagatga 194820 gaaactgctc aatttttcta tatcaccctc tccacccttt gcctgggata agaggaggta 194880 acccagcttt tcccacactg agcagaggtg acctgcctct ctctaatgaa ctgcgcttta 194940 tgcctgtttt gttcctgaga gaagagtttg gatagaggag gattaaagtt tgttttaaga 195000 gacccttcct gcagggtata gtggctcatg cctgtaatcc cagcactttg ggaggccaag 195060 acaagaggat tgcttaagcc caggagtttg agaccagcca gggcaacgca ggaggacacc 195120 cccatctcta caaatattta aaaaaaaaaa aatagctgag catggcagta cacacttgta 195180 gtcccaagta gcaactcagg aggctgaggt aggaggatca cttgaacctg cgaagtggag 195240 gctgcagtga gttatcattg taccagtgta ctcacctggg caacatagca agaccctatc 195300 tctacaaaaa taaaaataaa aattagctgt gtatggtggt gtgcacctgt agtcctagct 195360 actcaggagg ctgaggtggg aagattgctt gagcccagga gtttgaggct gcagtgaact 195420 attactatgc cactgcactc cagcctgggc aaccgagcaa gacctaatct ctataaaaat 195480 gaaaaagacc cttccttcca acaacgttaa accactagag atgtttcgta ctccctacaa 195540 ttttttctct ctttctgttc acaatacatt gggacttaca ggcagagtaa gagtcaatgg 195600 aagctatggt aagaaagtca acagagggct ggaggaacaa taccaacagc taggattctc 195660 tactcccaga tttgctaaca agcattgatt tgctccactg atatacaaca caatctgagg 195720 gaagatcatt gaaaatctag gatcttttgg tctaaagttg gaatcatggg gaggcatttt 195780 ctcaaccttg ttcatgtctc tttcctttgc agggcaacag gctttggctt cttaaatgcg 195840 ctatgcaagg cagcagccgt cctgggaaac ttaatatttg gctctctggt cagcatcacc 195900 aaatcaatcc ccatcctgct ggcttctact gtgctcgtgt gtggaggact cgttgggctg 195960 tgcctgcctg acacacgaac ccaggttctg atgtaatggg aaaaaaagcc atccttcctg 196020 cgtttcttcc tcctgccctg ggtcaattct ccttcctgac tcaaggcttc agagttttcc 196080 tatatagaaa ggtgatcaag tatcagaaca taaacacgtg ctgtgactta aaatttagaa 196140 gcatatcatc ttgccccttt gtgattttgc acaggttgtt tgtttgtttt gttttgtttg 196200 aatgcattgt tatctttcca aactgtgatg ctactgcctc ccgcaaatca gtggaagcat 196260 ttctaaaatg ccctaggcag ccaggcttcc ctggggttct gctcattgaa agtttagaag 196320 ctaagaagac tgggctggtg taagaaatag atcctgctcc taatttaaca ttaacaggaa 196380 atataagtcg gcacattatt gcatttgtgc tgagaagagg gattcttttt tttttttttt 196440 ctaacagagt gatatttcct gtttacttag aaaaggacac ccagtaattc tcactgttat 196500 agcagagcct ttcaaagaaa accatgtggc accaaacagg gctgggtggg gctctttggg 196560 gcaagacaat taaggtgacc gctgacaaga aaaataaaag ctctgcaatt agcagcaaaa 196620 atctgagtgt gctgcataag caagatttct gtcagaaagc ccaattaaac ctctgaaaag 196680 tgtgaggaaa aagaactgta acagtatctg tgactgtggc atgtggttca gaatgtttga 196740 aaatcagaga tccgagagag agcttctgcc tccatttacc actcccactc cttcagctgc 196800 ccttggtctt gccagacagc agattcaggg aaagaaagct gcttcttaaa aactgtctgc 196860 ctacctgatt ctgccactta cccacactgt ctctagtcta gagattgcca gtggatagct 196920 aaattgtaaa ttgcaaatta ggaagaaatt cacacccaga gccatttgga aatacaaaat 196980 ggtacctctt tccagtacct ctttgctgtg ggactggccc gccaggaccc ccaccccacc 197040 tggcccgcag ttgacattca gcttcccagc tggcatcagc aaagagggtt tgtccctcta 197100 gcttccccag ggctggccca ctgctcaatg cgctcctggc tccctgtaaa atcacagccc 197160 gcctgcactc ctgagggccc ctctcacaga cagaccatct ccctgtggtt gtttgactgc 197220 tcattttacc tctggaatct gcctgggctt ggaagaaaga ggtcagccag gacattccca 197280 cgggcccgct gtcagctaca tgaccttctc gcctctcagg ctccccttcc cccaggctcg 197340 tcctttttac acctcttctt tgaaatctga accttccttg tagacttgca gccaatgacc 197400 agaagccagg aataaatccc atatttaaac aatattcctt ttttaactgc ctcgatagag 197460 gtttattctt gttacttatt ataggcattt tcaggcaagc catagcttgg ggcctccagg 197520 aagggaaagc ttttgtttcc actgatcatt attttcagag atgtcctaac tccccctgag 197580 gcccccctgc ctccccctcc ccttcctaat gatccctcgc agagctcaga ctaagtgaaa 197640 agcttctttc attaacataa gccattaatc gacttcccct tgaagtaatt caggtctgca 197700 gaggggggaa gactggtgtt gggggcagtg ggaagagggt ggttccctgg gggtctgtga 197760 tcagggctag aacatcagga aaacccgaac caaaaactgc aaaggtggca ggaagaagaa 197820 aaatcactga aggctagaca agatgcattt gaaagatacc aaccgcacag ggaaagaaac 197880 aactttctct cacttctgcc tcctatcatt ttattggaga acaaagacag cagtcaaaca 197940 acccaagaaa gtaccctgta tgtttttctt cttccacatg tacccatttt cccacct 197997 4 727 PRT Rattus norvegicus 4 Met Glu Asp Ser Tyr Lys Asp Arg Thr Ser Leu Met Lys Gly Ala Lys 1 5 10 15 Asp Ile Ala Lys Glu Val Lys Lys Gln Thr Val Lys Lys Val Asn Gln 20 25 30 Ala Val Asp Arg Ala Gln Asp Glu Tyr Thr Gln Arg Ser Tyr Ser Arg 35 40 45 Phe Gln Asp Glu Asp Asp Asp Asp Asp Tyr Tyr Pro Pro Gly Glu Thr 50 55 60 Tyr Ser Gly Glu Ala Asn Asp Asp Glu Gly Ser Ser Glu Ala Thr Glu 65 70 75 80 Gly His Asp Glu Glu Asp Glu Ile Tyr Glu Gly Glu Tyr Gln Gly Ile 85 90 95 Pro Ser Thr Asn Gln Gly Lys Asp Ser Ile Val Ser Val Gly Gln Pro 100 105 110 Lys Gly Asp Glu Tyr Lys Asp Arg Arg Glu Leu Glu Ser Glu Arg Arg 115 120 125 Ala Asp Glu Glu Glu Leu Ala Gln Gln Tyr Glu Leu Ile Ile Gln Glu 130 135 140 Cys Gly His Gly Arg Phe Gln Trp Ala Leu Phe Phe Val Leu Gly Met 145 150 155 160 Ala Leu Met Ala Asp Gly Val Glu Val Phe Val Val Gly Phe Val Leu 165 170 175 Pro Ser Ala Glu Thr Asp Leu Cys Ile Pro Asn Ser Gly Ser Gly Trp 180 185 190 Leu Gly Ser Ile Val Tyr Leu Gly Met Met Val Gly Ala Phe Phe Trp 195 200 205 Gly Gly Leu Ala Asp Lys Val Gly Arg Lys Gln Ser Leu Leu Ile Cys 210 215 220 Met Ser Val Asn Gly Phe Phe Ala Phe Leu Ser Ser Phe Val Gln Gly 225 230 235 240 Tyr Gly Phe Phe Leu Leu Cys Arg Leu Leu Ser Gly Phe Gly Ile Gly 245 250 255 Gly Ala Ile Pro Thr Val Phe Ser Tyr Phe Ala Glu Val Leu Ala Arg 260 265 270 Glu Lys Arg Gly Glu His Leu Ser Trp Leu Cys Met Phe Trp Met Ile 275 280 285 Gly Gly Ile Tyr Ala Ser Ala Met Ala Trp Ala Ile Ile Pro His Tyr 290 295 300 Gly Trp Ser Phe Ser Met Gly Ser Ala Tyr Gln Phe His Ser Trp Arg 305 310 315 320 Val Phe Val Ile Val Cys Ala Leu Pro Cys Val Ser Ser Val Val Ala 325 330 335 Leu Thr Phe Met Pro Glu Ser Pro Arg Phe Leu Leu Glu Val Gly Lys 340 345 350 His Asp Glu Ala Trp Met Ile Leu Lys Leu Ile His Asp Thr Asn Met 355 360 365 Arg Ala Arg Gly Gln Pro Glu Lys Val Phe Thr Val Asn Lys Ile Lys 370 375 380 Thr Pro Lys Gln Ile Asp Glu Leu Ile Glu Ile Glu Ser Asp Thr Gly 385 390 395 400 Thr Trp Tyr Arg Arg Cys Phe Val Arg Ile Arg Thr Glu Leu Tyr Gly 405 410 415 Ile Trp Leu Thr Phe Met Arg Cys Phe Asn Tyr Pro Val Arg Glu Asn 420 425 430 Thr Ile Lys Leu Thr Ile Val Trp Phe Thr Leu Ser Phe Gly Tyr Tyr 435 440 445 Gly Leu Ser Val Trp Phe Pro Asp Val Ile Lys His Leu Gln Ser Asp 450 455 460 Glu Tyr Ala Leu Leu Thr Arg Asn Val Gln Lys Asp Lys Tyr Ala Asn 465 470 475 480 Phe Ser Ile Asn Phe Thr Met Glu Asn Gln Val His Thr Gly Met Glu 485 490 495 Tyr Asp Asn Gly Arg Phe Leu Gly Val Lys Phe Lys Ser Val Thr Phe 500 505 510 Lys Asp Ser Val Phe Lys Ser Cys Thr Phe Asp Asp Val Thr Ser Val 515 520 525 Asn Thr Tyr Phe Lys Asn Cys Thr Phe Ile Asp Thr Leu Phe Glu Asn 530 535 540 Thr Asp Phe Glu Pro Tyr Lys Phe Ile Asp Ser Glu Phe Gln Asn Cys 545 550 555 560 Ser Phe Leu His Asn Lys Thr Gly Cys Gln Ile Thr Phe Asp Asp Asp 565 570 575 Tyr Ser Ala Tyr Trp Ile Tyr Phe Val Asn Phe Leu Gly Thr Leu Ala 580 585 590 Val Leu Pro Gly Asn Ile Val Ser Ala Leu Leu Met Asp Arg Ile Gly 595 600 605 Arg Leu Thr Met Leu Gly Gly Ser Met Val Leu Ser Gly Ile Ser Cys 610 615 620 Phe Phe Leu Trp Phe Gly Thr Ser Glu Ser Met Met Ile Gly Met Leu 625 630 635 640 Cys Leu Tyr Asn Gly Leu Thr Ile Ser Ala Trp Asn Ser Leu Asp Val 645 650 655 Val Thr Val Glu Leu Tyr Pro Thr Asp Arg Arg Ala Thr Gly Phe Gly 660 665 670 Phe Leu Asn Ala Leu Cys Lys Ala Ala Ala Val Leu Gly Asn Leu Ile 675 680 685 Phe Gly Ser Leu Val Ser Ile Thr Lys Ala Ile Pro Ile Leu Leu Ala 690 695 700 Ser Thr Val Leu Val Cys Gly Gly Leu Val Gly Leu Arg Leu Pro Asp 705 710 715 720 Thr Arg Thr Gln Val Leu Met 725 

That which is claimed is:
 1. An isolated peptide consisting of an amino acid sequence selected from the group consisting of: (a) an amino acid sequence shown in SEQ ID NO:2; (b) an amino acid sequence of an allelic variant of an amino acid sequence shown in SEQ ID NO:2, wherein said allelic variant is encoded by a nucleic acid molecule that hybridizes under stringent conditions to the opposite strand of a nucleic acid molecule shown in SEQ ID NOS:1 or 3; (c) an amino acid sequence of an ortholog of an amino acid sequence shown in SEQ ID NO:2, wherein said ortholog is encoded by a nucleic acid molecule that hybridizes under stringent conditions to the opposite strand of a nucleic acid molecule shown in SEQ ID NOS:1 or 3; and (d) a fragment of an amino acid sequence shown in SEQ ID NO:2, wherein said fragment comprises at least 10 contiguous amino acids.
 2. An isolated peptide comprising an amino acid sequence selected from the group consisting of: (a) an amino acid sequence shown in SEQ ID NO:2; (b) an amino acid sequence of an allelic variant of an amino acid sequence shown in SEQ ID NO:2, wherein said allelic variant is encoded by a nucleic acid molecule that hybridizes under stringent conditions to the opposite strand of a nucleic acid molecule shown in SEQ ID NOS:1 or 3; (c) an amino acid sequence of an ortholog of an amino acid sequence shown in SEQ ID NO:2, wherein said ortholog is encoded by a nucleic acid molecule that hybridizes under stringent conditions to the opposite strand of a nucleic acid molecule shown in SEQ ID NOS:1 or 3; and (d) a fragment of an amino acid sequence shown in SEQ ID NO:2, wherein said fragment comprises at least 10 contiguous amino acids.
 3. An isolated antibody that selectively binds to a peptide of claim
 2. 4. An isolated nucleic acid molecule consisting of a nucleotide sequence selected from the group consisting of: (a) a nucleotide sequence that encodes an amino acid sequence shown in SEQ ID NO:2; (b) a nucleotide sequence that encodes of an allelic variant of an amino acid sequence shown in SEQ ID NO:2, wherein said nucleotide sequence hybridizes under stringent conditions to the opposite strand of a nucleic acid molecule shown in SEQ ID NOS:1 or 3; (c) a nucleotide sequence that encodes an ortholog of an amino acid sequence shown in SEQ ID NO:2, wherein said nucleotide sequence hybridizes under stringent conditions to the opposite strand of a nucleic acid molecule shown in SEQ ID NOS:1 or 3; (d) a nucleotide sequence that encodes a fragment of an amino acid sequence shown in SEQ ID NO:2, wherein said fragment comprises at least 10 contiguous amino acids; and (e) a nucleotide sequence that is the complement of a nucleotide sequence of (a)-(d).
 5. An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of: (a) a nucleotide sequence that encodes an amino acid sequence shown in SEQ ID NO:2; (b) a nucleotide sequence that encodes of an allelic variant of an amino acid sequence shown in SEQ ID NO:2, wherein said nucleotide sequence hybridizes under stringent conditions to the opposite strand of a nucleic acid molecule shown in SEQ ID NOS:1 or 3; (c) a nucleotide sequence that encodes an ortholog of an amino acid sequence shown in SEQ ID NO:2, wherein said nucleotide sequence hybridizes under stringent conditions to the opposite strand of a nucleic acid molecule shown in SEQ ID NOS:1 or 3; (d) a nucleotide sequence that encodes a fragment of an amino acid sequence shown in SEQ ID NO:2, wherein said fragment comprises at least 10 contiguous amino acids; and (e) a nucleotide sequence that is the complement of a nucleotide sequence of (a)-(d).
 6. A gene chip comprising a nucleic acid molecule of claim
 5. 7. A transgenic non-human animal comprising a nucleic acid molecule of claim
 5. 8. A nucleic acid vector comprising a nucleic acid molecule of claim
 5. 9. A host cell containing the vector of claim
 8. 10. A method for producing any of the peptides of claim 1 comprising introducing a nucleotide sequence encoding any of the amino acid sequences in (a)-(d) into a host cell, and culturing the host cell under conditions in which the peptides are expressed from the nucleotide sequence.
 11. A method for producing any of the peptides of claim 2 comprising introducing a nucleotide sequence encoding any of the amino acid sequences in (a)-(d) into a host cell, and culturing the host cell under conditions in which the peptides are expressed from the nucleotide sequence.
 12. A method for detecting the presence of any of the peptides of claim 2 in a sample, said method comprising contacting said sample with a detection agent that specifically allows detection of the presence of the peptide in the sample and then detecting the presence of the peptide.
 13. A method for detecting the presence of a nucleic acid molecule of claim 5 in a sample, said method comprising contacting the sample with an oligonucleotide that hybridizes to said nucleic acid molecule under stringent conditions and determining whether the oligonucleotide binds to said nucleic acid molecule in the sample.
 14. A method for identifying a modulator of a peptide of claim 2, said method comprising contacting said peptide with an agent and determining if said agent has modulated the function or activity of said peptide.
 15. The method of claim 14, wherein said agent is administered to a host cell comprising an expression vector that expresses said peptide.
 16. A method for identifying an agent that binds to any of the peptides of claim 2, said method comprising contacting the peptide with an agent and assaying the contacted mixture to determine whether a complex is formed with the agent bound to the peptide.
 17. A pharmaceutical composition comprising an agent identified by the method of claim 16 and a pharmaceutically acceptable carrier therefor.
 18. A method for treating a disease or condition mediated by a human transporter protein, said method comprising administering to a patient a pharmaceutically effective amount of an agent identified by the method of claim
 16. 19. A method for identifying a modulator of the expression of a peptide of claim 2, said method comprising contacting a cell expressing said peptide with an agent, and determining if said agent has modulated the expression of said peptide.
 20. An isolated human transporter peptide having an amino acid sequence that shares at least 70% homology with an amino acid sequence shown in SEQ ID NO:2.
 21. A peptide according to claim 20 that shares at least 90 percent homology with an amino acid sequence shown in SEQ ID NO:2.
 22. An isolated nucleic acid molecule encoding a human transporter peptide, said nucleic acid molecule sharing at least 80 percent homology with a nucleic acid molecule shown in SEQ ID NOS:1 or
 3. 23. A nucleic acid molecule according to claim 22 that shares at least 90 percent homology with a nucleic acid molecule shown in SEQ ID NOS:1 or
 3. 